Drp1-JNKknockdown mitigates Scribble loss induced cell proliferation, metastasis and lethality phenotypes inDrosophila

Drp1-JNK knockdown mitigates Scribble loss induced cell proliferation, metastasis and lethality phenotypes in Drosophila | bioRxiv /* */ /* */ <!-- <!-- /*! * yepnope1.5.4 * (c) WTFPL, GPLv2 */ (function(a,b,c){function d(a){return"[object Function]"==o.call(a)}function e(a){return"string"==typeof a}function f(){}function g(a){return!a||"loaded"==a||"complete"==a||"uninitialized"==a}function h(){var a=p.shift();q=1,a?a.t?m(function(){("c"==a.t?B.injectCss:B.injectJs)(a.s,0,a.a,a.x,a.e,1)},0):(a(),h()):q=0}function i(a,c,d,e,f,i,j){function k(b){if(!o&&g(l.readyState)&&(u.r=o=1,!q&&h(),l.onload=l.onreadystatechange=null,b)){"img"!=a&&m(function(){t.removeChild(l)},50);for(var d in y[c])y[c].hasOwnProperty(d)&&y[c][d].onload()}}var j=j||B.errorTimeout,l=b.createElement(a),o=0,r=0,u={t:d,s:c,e:f,a:i,x:j};1===y[c]&&(r=1,y[c]=[]),"object"==a?l.data=c:(l.src=c,l.type=a),l.width=l.height="0",l.onerror=l.onload=l.onreadystatechange=function(){k.call(this,r)},p.splice(e,0,u),"img"!=a&&(r||2===y[c]?(t.insertBefore(l,s?null:n),m(k,j)):y[c].push(l))}function j(a,b,c,d,f){return q=0,b=b||"j",e(a)?i("c"==b?v:u,a,b,this.i++,c,d,f):(p.splice(this.i++,0,a),1==p.length&&h()),this}function k(){var a=B;return a.loader={load:j,i:0},a}var l=b.documentElement,m=a.setTimeout,n=b.getElementsByTagName("script")[0],o={}.toString,p=[],q=0,r="MozAppearance"in l.style,s=r&&!!b.createRange().compareNode,t=s?l:n.parentNode,l=a.opera&&"[object Opera]"==o.call(a.opera),l=!!b.attachEvent&&!l,u=r?"object":l?"script":"img",v=l?"script":u,w=Array.isArray||function(a){return"[object Array]"==o.call(a)},x=[],y={},z={timeout:function(a,b){return b.length&&(a.timeout=b[0]),a}},A,B;B=function(a){function b(a){var a=a.split("!"),b=x.length,c=a.pop(),d=a.length,c={url:c,origUrl:c,prefixes:a},e,f,g;for(f=0;f<d;f++)g=a[f].split("="),(e=z[g.shift()])&&(c=e(c,g));for(f=0;f<b;f++)c=x[f](c);return c}function g(a,e,f,g,h){var i=b(a),j=i.autoCallback;i.url.split(".").pop().split("?").shift(),i.bypass||(e&&(e=d(e)?e:e[a]||e[g]||e[a.split("/").pop().split("?")[0]]),i.instead?i.instead(a,e,f,g,h):(y[i.url]?i.noexec=!0:y[i.url]=1,f.load(i.url,i.forceCSS||!i.forceJS&&"css"==i.url.split(".").pop().split("?").shift()?"c":c,i.noexec,i.attrs,i.timeout),(d(e)||d(j))&&f.load(function(){k(),e&&e(i.origUrl,h,g),j&&j(i.origUrl,h,g),y[i.url]=2})))}function h(a,b){function c(a,c){if(a){if(e(a))c||(j=function(){var a=[].slice.call(arguments);k.apply(this,a),l()}),g(a,j,b,0,h);else if(Object(a)===a)for(n in m=function(){var b=0,c;for(c in a)a.hasOwnProperty(c)&&b++;return b}(),a)a.hasOwnProperty(n)&&(!c&&!--m&&(d(j)?j=function(){var a=[].slice.call(arguments);k.apply(this,a),l()}:j[n]=function(a){return function(){var b=[].slice.call(arguments);a&&a.apply(this,b),l()}}(k[n])),g(a[n],j,b,n,h))}else!c&&l()}var h=!!a.test,i=a.load||a.both,j=a.callback||f,k=j,l=a.complete||f,m,n;c(h?a.yep:a.nope,!!i),i&&c(i)}var i,j,l=this.yepnope.loader;if(e(a))g(a,0,l,0);else if(w(a))for(i=0;i (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0];var j=d.createElement(s);var dl=l!='dataLayer'?'&l='+l:'';j.src='//www.googletagmanager.com/gtm.js?id='+i+dl;j.type='text/javascript';j.async=true;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-M677548'); Skip to main content Home About Submit ALERTS / RSS Search for this keyword Advanced Search New Results Drp1-JNK knockdown mitigates Scribble loss induced cell proliferation, metastasis and lethality phenotypes in Drosophila Jyotsna Singh , Saripella Srikrishna doi: https://doi.org/10.1101/2024.04.10.588977 Jyotsna Singh 1 Cancer and Neurobiology Laboratory, Institute of Science, Banaras Hindu University , Varanasi- 221005, India Find this author on Google Scholar Find this author on PubMed Search for this author on this site Saripella Srikrishna 1 Cancer and Neurobiology Laboratory, Institute of Science, Banaras Hindu University , Varanasi- 221005, India Find this author on Google Scholar Find this author on PubMed Search for this author on this site For correspondence: skrishna{at}bhu.ac.in Abstract Full Text Info/History Metrics Preview PDF Abstract Mitochondrial dynamics are emerging as master regulators for targeting several types of cancers, including breast cancer, cervical cancer, and hepatocellular carcinoma, for therapeutic intervention. Mitochondrial morphology, size, position and activity within cells is regulated by dynamic fission and fusion events. Dynamin-related protein 1 ( Drp1 ) promotes mitochondrial fission and maintains mitochondrial homeostasis. Loss of Scrib is implicated in several human cancers wherein mitochondrial dysfunction leads to excessive cell proliferation and metastasis. However, the exact molecular mechanisms behind the Scrib loss induced dysregulation of mitochondrial dynamics in cancer progression remains obscure. Although the role of mitochondrial dynamics are being investigated in several types of cancers, but the role of Drp1 - mediated fission event in regulating the maintenance of polarity of cells upon loss of Scrib function is elusive. In this study, for the first time, we blocked the function of Drp1 activity in Scrib knockdown induced metastasis cancer model by two ways, firstly, through genetic ablation of Drp1, and secondly by using mdivi-1, a Drp1 specific inhibitor. Genetic depletion of Drp1 expression ( Drp1 RNAi ) in Scrib knockdown cells inhibits Metalloproteinase MMP1 , reduces ROS production, restores apico-basal (A/B) cell polarity and enhances ATP production. Further to confirm role of Drp1 in regulation of cell polarity, we employed mdivi, a Drp1 specific inhibitor which has dose dependent effect in cell polarity regulation. This study also reveals that JNK inhibition ( JNK RNAi ) in Scrib abrogated cells mitigates the Drp1 expression and controls cell proliferation leading to restoration of mitochondrial morphology and epithelial cellpolarity. Our results highlight Drp1 as a key regulator in maintaining the apico-basal polarity of cells which gets affected upon loss of Scrib but Drp1-JNK downregulation effectively mitigates Scrib RNAi associated cell proliferation, metastasis and pupal lethality phenotypes. Introduction Scribble ( Scrib ), is a tumor suppressor gene and apico-basal (A/B) polarity regulator in epithelial cells [ 1 ]. Many cancers of epithelial origin like cervical cancer [ 2 ], breast cancer [ 3 , 4 ], ovarian cancer, colorectal cancer, pituitary tumor etc., havebeen reported to exhibit mitochondrial dysfunction [ 5 ] but the underlying mechanismremains elusive. Mitochondrial disruption is the major source of increasedreactive oxygen species(ROS)production [ 6 ]. Loss of Scrib is associated with ROS enhancement in cancer cells, however, the regulatory mechanisms are largely unknown [ 7 ]. According to several studies, abnormal cell proliferation needs excessive production of ROS in order to improve cancer cell growth by an oncogenic cooperative signal [ 3 , 8 ]. In normal cells, the dynamic behavior of mitochondria isimportant for maintenance of cellular homeostasis [ 9 ]. The dynamics of mitochondrialmembrane machinery maintain the balance between the mitochondrial fission and fusion transition events [ 10 ]. The fusion of outer mitochondrial membrane (OMM) is regulated by mitofusin/Marf (Mitochondrial Assembly Regulatory Factor) in Drosophila , and Mfn (mitofusin) in mammals while inner mitochondrial membrane (IMM) fusion is regulated by optic atrophy 1 (OPA1) and mitochondrial fission is regulated by dynamin-related protein 1 ( Drp1 ). These two mitochondrial regulators encode a dynamin-family GTPase, to maintain the mitochondrial morphology and activity [ 11 ]. The Drp1 migrates from cytosol to mitochondrial outer membrane (MOM) and forms anoligomeric collar like structure around the mitochondrion and hydrolyzes GTP to execute Drp1 -mediated mitochondrial fission event [ 12 , 13 ]. In the current study, we have explored the role of Drp1 in tumor progression upon knockdown of Scrib in Drosophila . Our previous studies have shown that upregulation of Drp1 expression leads to mitochondrial dysfunction in Scrib knockdown tumor cells [ 14 ]. However, so far, there are no reports available, that demonstrate that in vivo blocking of Drp1 expressionin the background of Scrib loss of function are associated with cancer cell proliferation in Drosophila . For the first time we show that knockdown of Drp1 expression in Scrib knockdown cells results in restoration of cell polarity and rescues the pupal death phenotype. Moreover, to further determine the role of Drp1 in cancer cell migration and invasion upon Scrib knockdown, we employed Mdivi (Mitochondrial division inhibitor), a known inhibitor of Drp1, which inhibits its GTPase activity [ 15 ]. Our results shows Mdivi-1 induces a dose-dependent inhibition on cancer cell migration and restores the cell polarity upon loss of Scrib . In Drosophila , well conserved JNK (c-Jun N-terminal kinase) signaling pathway activation promotes cell proliferation upon Ras activation and cooperative association with cell polarity regulator genes [ 16 , 17 ]. JNK pathway’s activation leads to mitochondrial dysfunction and excess ROS production due to the translocation of JNK protein on to the outer membrane of mitochondria in cancer cells [ 18 , 19 ]. Previous studies report that JNK mediates Drp1 translocation to regulate mitochondrial fission event [ 20 ]. In this context, our studies show that Scrib regulates Drp1/JNK activity to maintain polarity of cells and regulates mitochondrial dynamics. To the best of our understanding, this study reveals a new insight into the mechanism for maintaining mitochondrial morphology and A/B cell polarity via the Drp1 dependent mitochondrial fission in Scrib abrogated tumor cells. Materials and Methods Fly stocks and culture conditions We used Oregon-R +/+ as a Wild type, UAS- Scrib RNAi (#35748),UAS-GFP (#1521), UAS- Drp1 RNAi (#51483), UAS- Hep RNAi (#2190-R2), Sp/Cyo; dCO2/TM6B, mitocherry Red OMM; Ptc-Gal4 and Ptc-GAL4 (#2017) fly strains for this study. The Ptc-gal4 driver linedrives the expression of the transgene at the anterior/posterior (A/P) boundary region of Drosophila wing imaginal discs. Flies strain were reared on corn agar medium that contained in 1000ml water: 5.6g of agar, 47.2g of Maize powder, 41.67g of sugar, 16.7g of yeast, 6.94ml absolute ethanol solution used to dissolve 2.8g of nepagin (anti-fungal) and 2.8ml of propionic acid (anti- bacterial). Fly stocks were obtained from the Bloomington Drosophila Stock Center ( https://flybase.bio.india na.edu). All crosses were carried out at 24 0 C in standard corn-meal agar media and fly stocks were maintained in B.O.D incubator. Wing disc area quantification The roaming third instar larvae of Wild type, Scribble knockdown ( Scribble RNAi ) and rescued genotype ( UAS-Drp1 RNAi ; UAS-Scrib RNAi and UAS-Hep RNAi ; UAS-Scrib RNAi ), were dissected in 1X PBS on cavity slide and separated wing imaginal discs which were transferred to the maximo cavity slide. Then the discs were fixed in 4% paraformaldehyde for 15 minutes at room temperature, followed by washing it with 1XPBST (0.1%), 3 times for 5 min each. The discs were mounted on glass slides with DABCO respectively. Bright flied images were obtained by confocal microscope (Zeiss LSM-510 Meta). To measure wing disc area using open free shape curve drawing mode in overlay option in offline LSM software (indicate by yellow line). The histogram represents average wing imaginal area disc for each genotype. Generation of transgenic flies Different crosses were set to bring Drp1 RNAi and Hep RNAi in genetic combination (introgression) together with Scrib RNAi . Also, to monitor tumor progression in wing imaginal disc, we genetically labeled Scrib knockdown cells using wing discs specific Ptc-Gal4 with a visible marker such as green fluorescent protein (GFP) (Supplementary Information, Scheme 1-4). Wing Venation pattern analysis Rescued adult flies were anaesthetized and the wings from the thorax region were carefully detached with the help of sharp dissection needles. The isolated wing was placed on the top of slide, protected with a coverslip, the edges of which were completely sealed with a transparent nail polish. The images for wing venation pattern were captured under bright-field filter using fluorescence Nikon-NiU microscope. The length of L2 to ACV, length of ACV that bridge L3- L4 and PCV that bridge L4-L5 is measured with offline NIS-element BR 4.3 software for alteration analysis. Lifespan Assay The lifespan of adult rescue flies of UAS-Drp1 RNAi ; UAS-Scrib RNAi and UAS-Hep RNAi ; UAS- Scrib RNAi genotypes were measured from the day of eclosion at 24°C. A minimum of 100 flies were taken (10 flies/vial) for each genotype. The total number of surviving flies were checked and counted every day. The flies were transferred in fresh food vials every 2 days, the number of dead flies was scored for each genotypes and survival curve generated by using Graph pad prism 5 software. Drug screening in Cancer bearing larvae To test whether Drp1 inhibition positively regulates the polarity of cells, we tested the effect of small chemical molecule Drp1 inhibitor, mdivi on Scrib knockdown tumor bearing larvae. The mdivi was obtained from Sigma-Aldrich (M0199), pre-dissolved in DMSO (dimethylsulfoxide) and mixed in corn agar food medium. 100% DMSO shows toxic effect on flies, so, we prepared drug solution in 0.3 % DMSO considered as no observed adverse effect level (NOAEL), non- toxic concentration used for the in-vivo drug screening in Drosophila melanogaster [ 21 , 22 ]. Control flies treated with DMSO and without DMSO were used for the experiment. The cancer bearing larvae were fed with drug treated food from higher to lower concentration. Drug treatment was analyzed at six different concentrations, 15µM, 30µM, 60µM, 120µM, 250µM and 500µM to test dose dependent effect tumor development. As 15µM and 30µM drug treated larvae, did not show any phenotypic variation in tumor growth, so further experimental analysis where performed at above 30µM drug doses. Immunostaining of larval wing imaginal discs The Drosophila third instar larval wing imaginal discs were isolated from the desired genotypes. Dissection was performed in 1X PBS (phosphate buffer saline) solution. Tissue were fixed in 4% paraformaldehyde (PFA) for 15 min at RT. The wing discs were washed in 0.2% PBST (1X PBS, 0.2% Triton-X) three times, 5 min each and tissue were blocked in blocking solution (3% bovine serum albumin (BSA) solution in 1X PBS) for 1hr at RT followed by incubation with the required primary antibodyin blocking solution for overnight at 4°C. A cocktail of three mouse anti-MMP1 (matrix metalloproteinases-1) catD monoclonal antibodies raised against the catalytic domain (14A3D2, 3B8D12, 5H7B11 used in 1:1:1 dilution) was obtained from the developmental study hybridoma bank (DSHB), Iowa. Rabbit anti-Drp1 (FITC-DRP1 used 1:100 dilution) was obtained from the FABGENIX, Rabbit anti-ACTIVE JNK pAb, (v7931 used in 1:1000 dilution) from Promega and Rabbit anti-Ph3 was obtained from Merck Millipore (06-570 used in 1:1000 dilution). The tissues were washed with 0.2% PBST three times, 5 min each, followed by incubation with the desired secondary antibodyin 1X PBS for 3hrs at RT. Secondary antibodies used were, goat anti-mouse IgG, Alexa Fluor 594 (1:1000 dilution, Cat# A-11005) and goat anti-rabbit IgG, Alexa Fluor 488 (1:1000 dilution, Cat# A-11008). Tissues were washed with 0.1% PBST three times, 5 min each following the 2 0 incubation, further incubated with DAPI (1µg/ml in 1X PBS) for 10min then again washed with 0.1% 1X PBST (1X PBS, 0.1% Triton-X) three times, 5 min each and then sample were mounted in DABCO. Immunofluorescence images were taken using confocal microscope (Zeiss LSM-510 Meta) and processed using LSM software and arranged in Adobe Photoshop 7.0. RNA isolation and cDNA synthesis Total RNA was extracted from desired genotype (Wild type (Oregon R +) , Scribble RNAi and rescued genotype ( UAS-Drp1 RNAi ; UAS-Scrib RNAi and UAS-Hep RNAi ; UAS-Scrib RNAi ), using TRI reagent (TAKARA) as per manufacturer’s protocol. The quantity of RNA was checked on a 1% agarose gel and documented using Gel Doc (BIO-RAD). High quantity RNA from both samples estimated by absorbance A260/280 ratio was reverse transcribed to cDNA. For cDNA preparation 1µg of RNA, 1µl random hexamer (Applied Biosystems) and 11µl (0.1%) DEPC treated water was run for single cycle at 70 0 C for 5min. in a thermal cycler (BIO-RAD). To this 2µl 10mM dNTPs (Invitrogen) and 0.5µl (200U/µl) RNase inhibitor (Applied Biosystems) were added and run for single cycle at 25 0 C for 5 min. Finally, 1µl (100U/µl) reverse transcriptase (Ambion) was added and whole mixture was run at 37 0 C for 1h in a thermal cycler (Sure Cycler 8800, Agilent). Qualitative Real-time PCR (qRT-PCR) Prepared cDNA of desired genotype was used as a template for quantification of differentially expressed genes, normalized against GAPDH with specific primers (Table.1) using SYBR (R) GREEN JUMPSTART TAQ Ready mix (Thermo Fisher Scientific) using Real-time thermal cycler analysis (ΔCT values). View this table: View inline View popup Download powerpoint Table. 1. Specific primers (shown in 5’-3’ direction) for different genes. Western blotting Total protein was extracted using lysis buffer and protein quantification was done with Bradford assay. Equal amount of protein from defined experimental groups were loaded and resolved on the 10% SDS-PAGE,wet transferred onto PVDF membrane at 4°C. Blot was probed with primary antibodies with overnight incubation. The antibodies used are, a cocktail of three monoclonal mouse antibodies for MMP1 (1:100, DSHB, Cat No. 3A6B4, 3B8D12 and 5H7B11), polyclonal rabbit antibody for Drp1 (1:500, Cat No. DRP1 FITC), and monoclonal mouse antibody for β-tubulin (1:125, Cat No.DSHB-S1-810-(DSHB)-E7 anti beta tubulin supernatant 1ml).Proteins were detected using Goat Anti-Mouse IgG (H+L) peroxidase Conjugates (1;2500, Thermo scientific, Cat No. 31430) and Goat Anti-Rabbit IgG-HRP (1;1000, Merck, Cat No. 032102). The specific bands were detected using the ECL (Enhanced chemiluminescence detection, Cat No. 1705060 (Genetix)-Clarity Western ECL Subs, 200ml) system. Gene expression was analyzed after normalizing β-tubulin expression using image J softwere. In silico Analysis Protein-Protein interaction signaling pathways were analyzed by STRING 9.0 web software. STRING is a functional protein connection networks analysis ( https://string-db.org ) using protein accession number to analyzed the connecting link between molecular signaling pathways and interaction associated between the differential expressed proteins [ 23 ]. ROS estimation To detect in-vivo intracellular ROS production in desired wing imaginal disc, we used H2DCFDA (2’,7’-dichlorodihydro fluorescein diacetate, Sigma, D6883) staining. H2DCFDA is a cell membrane permeable indicator used for detection of ROS. Oxidation of H2DCFDA, when it reacts with H2O2, generates highly green fluorescent 2’,7’-dichlorofluorescein (DCF). The acetate group H2DCFDA hydrolyzed by intracellular esterase converts non-fluorescent H2DCFDA into highly green fluorescent DCF [ 24 ]. Third instar larval wing imaginal discs of desired genotype were isolated in 1X PBS (pH7.2) and discs were incubated in H2DCFDA (working concentration 5µg/ml) for 30minute at 37°C, washed with 1X PBS and images were captured in fluorescence microscope and processed using software NIS-Elements (BR). Biochemical Assays SOD Assay by NBT method SOD assay was used for measuring the superoxide dismutase (SOD) enzyme inhibition activity. The samples of desired genotype were homogenized in 50µl extraction buffer (100mM K2HPO4, 100mM KH2PO4 and 1mM EDTA) and centrifuged at 12,000 rpm for 10 minute at 4°C. The supernatants were transferred to fresh 1.5ml micro centrifuge tubes and concentration of protein was estimated by Bradford method [ 25 ]. Further, in a clean glass tube 900µl distilled water, 200µl L-methionine (20mM) and 100µl NBT (2.25mM) (nitro blue tetrazolium),(which produce light blue color formazan after reducing the superoxide anion), and 1.5ml of extraction buffer were added for 100µg protein sample. In dark condition, sample was incubated for 30 minute under yellow light after adding 100µl riboflavin (60µM). Absorbance were taken at 560nm using multimode plate reader Synergy H1. Then to measure 50% inhibition of NBT reduction by superoxide dismutase (SOD) by subtraction the sample OD with blank OD then divide with blank OD (Blank OD-Sample OD/ Blank OD*100). 50% SOD inhibition is equal to 1 SOD unit then 1% equal to 1/50 multiple by 50% inhibition of NBT reduction. The SOD activity was represented by units per min per mg protein sample. H2O2 (Hydrogen peroxide) Assay The sample of desired genotype were homogenized in sodium phosphate buffer (50mM, pH 6.6) containing Na2HPO4 and NaH2PO4, and centrifuged at 10,000rpm for 10 minute at 4°C. The supernatants were transferred to fresh 1.5ml micro centrifuge tube and concentration of protein is estimated by Bradford method. In 100µg sample protein added 1ml TiSO4 (0.1% in 20% H2SO4) and incubated for 10 minute at room temperature. Further, it was centrifuged at 1000rpm for 20 minute at 4°C. Absorbance were taken at 410nm using multimode plate reader Synergy H1. Catalase (CAT) Assay The sample of desired genotype were homogenized in catalase extraction buffer (pH 8.0) containing Tris-HCl (50mM), EDTA (0.5mM) and 2% (w/v) PVP (polyvinyl pyrrolidone). The homogenized sample were centrifuge 10,000rpm for 10 minute at 4°C. The supernatants were transferred to fresh 1.5ml micro centrifuge tube and concentration of protein was estimated by Bradford method. The assay mixture contains 100µl crude total protein, 1ml potassium buffer (contain 100mM K2HPO4, 100mM KH2PO4) and 20mM of 8.8M H2O2 solution. The H2O2 consumption was measured 10times at 5 second interval and absorbance was recorded at 240nm. The catalase activity was calculated by subtracting highest value of absorbance to lowest value of absorbance 240nm divided by time log (5 sec) multiply by extinction coefficient 0.036. TBARS (Lipid peroxidation) Assay The Thiobarbituric acid reactive substance (TBARS) assay also known as lipid peroxidation assay is used for detection of lipid oxidation by measuring end product, the malondialdehyde (MDA). The sample of desired genotype were homogenized in homogenizing buffer (1XPBS (1ml), 10µl PMSF) and centrifuged at 10,000 rpm for 20 minute at 4°C. The supernatants were transferred to a fresh 1.5ml micro centrifuge tube and concentration of protein was estimated by Bradford method. In total extracted protein sample,1.6ml d.H2O, and 100µl SDS (10%) were added and after 5-minute incubation at RT, 20% acetic acid (600µl) was added and further incubated at RT for 2 minute. 0.8% TBA (Thiobarbituric acid) was added to the sample, mixed properly and placed in 90°C in water bath for 1hour at. Subsequently, the samples were cooled and centrifuged at 10,000rpm for 10minute at 4°C. Absorbance were taken at 532nm and 600nm using multimode plate reader Synergy H1. The MDA concentration calculation can be done by subtracting absorbance reading at 532nm to absorbance reading at 600nm divided by 155, which is extinction coefficient of MDA. Analysis of Mitochondrial structural morphology Mitochondrial structure was analyzed by employing Ptc-Gal4 driver line and UAS-mitocherry red (Standard genetic cross schemes shown in Scheme. 2). Wing imaginal discs from roaming third instar larvae of UAS-mitocherry; Ptc-Gal4, Scribble RNAi (UAS-mitocherry; Ptc- Gal4< Scribble RNAi ) and rescued genotype (UAS-mitocherry; Ptc-Gal4< UAS-Drp1 RNAi ; UAS- Scrib RNAi and UAS-mitocherry; Ptc-Gal4< UAS-Hep RNAi ; UAS-Scrib RNAi ), were dissected in IX PBS and were fixed in 4% paraformaldehyde for 15 minutes at room temperature, followed by washing with 1XPBST (0.1%), 3 times for 5 min each. The discs were mounted on glass slides with DABCO. Images were obtained by confocal microscope (Zeiss LSM-510 Meta). In-situ cell death detection (TUNEL) Assay Detection of cell death was carried out using In-situ cell death detection kit, TMR red (Roche Diagnostics, REF 12156792910). Wing imaginal discs from roaming third instar larvae of wild type, cancer Scribble RNAi and rescued genotype (UAS- Drp1 RNAi ; UAS-Scrib RNAi and UAS- Hep RNAi ; UAS-Scrib RNAi ), were dissected in 1X PBS and were fixed in 4% paraformaldehyde for 15 minutes, followed by washing it with 1XPBST, 3 times for 5 min each for permeabilization and washed three times with 1X PBS, 5 min each followed by incubation with TUNEL (TdT- mediated dUTP-X nick end labelling) reaction mixture at 37°C for 2 hrs followed by washing with 1XPBS for two times 5 min each. The working solution of TUNEL reaction mixture were prepared immediately just before use by mixing the 50µl of enzyme solution (TdT) in 450µl of label solution (TMR-dUTP). The discs were mounted on glass slides with DABCO. Images were obtained by confocal microscope (Zeiss LSM-510 Meta). ATP Consumption Assay The sample of desired genotype were homogenized in 50µl ATP assay buffer and centrifuged at 12,000 rpm for 15mintute at 4°C. The total ATP production was measure in nm/µg of protein by the ATP colorimetric assay kit (Sigma, MAK190-1KT) and experiment was performed as per provided manufacturer’s procedure. The developed pink color is stable for 2 hours. The absorbance was taken at 570nm in multimode microplate reader. The protein concentration of the sample was measure by the Bradford method and ATP level was normalized with protein content. Statistical Analysis The statistical analysis was performed using Graph Pad Prism 5.0 Software Inc. All data results with error bar represented as mean ± standard error mean (SEM) used for statistical analysis for three independent experiments. The data were analyzed by using one-way ANOVA (analysis of variances) followed by Bonferroni multiple comparison test and two-tailed student’s t test for real-time PCR shows statistical difference between wildtype and tested group of indicated genotypes. Survival assay or Life span assay was performed by Kaplan-Meier method and significant was calculated by Log-rank (Mantel-Cox) test. P<0.05 was considered a statistically significance difference for data analysis. Results Knockdown of Drp1 and JNK signaling in Scrib RNAi cells inhibit metastasis, polarity loss and absolute pupal lethality In previous reports, loss of Scrib is shown to exacerbate the cell polarity defects and disrupt cell- cell junction integrity [ 26 ]. We used the wing specific patched-Gal4 (Ptc-Gal4) to knockdown the expression of scrib through UAS-Scrib RNAi , specifically in anterior posterior boundary region in wing disc. We found that scrib knockdown leads to development of giant tumor bearing larvae (Ptc-Gal4>UAS- Scrib RNAi ) ( Fig.1 A and B). For a First, we show the rescue of scrib knockdown induced larval phenotypes upon Drp1 depletion (Ptc-Gal4>UAS- Drp1 RNAi ; UAS- Scrib RNAi ) ( Fig. 1 . C). Further, JNK signaling upstream regulator Hemipterous ( Hep ), also known as Jun Kinase Kinsae (JNKK), knockdown in Scrib abrogated cells (Ptc-Gal4> UAS-Hep RNAi ; UAS-Scrib RNAi ) suppresses tumor development ( Fig. 1 . D). The wing disc area from the Scrib knockdown larvae shows that the tumorous wing disc is smaller in size area wise with an increased volume compared to wild type ( Fig.1I ) and this phenotype is rescued upon knockdown of Drp1 and JNK signaling (UAS- Drp1 RNAi , UAS- Scrib RNAi and UAS-Hep RNAi; UAS-Scrib RNAi ) as shown in Fig.1 . (E-H). The wing disc area ( Fig.1 .I). Download figure Open in new tab Figure 1. Rescue of Scrib loss induced phenotype by knockdown of Drp1 and JNK . Mature 3 rd instar larvae from Wild type (A), Scrib RNAi (B), Drp1 RNAi ; Scrib RNAi (C) and Hep RNAi ;Scrib RNAi (D). Note the enlarged larval size of Scrib RNAi (B) in comparison to wild type (A) and rescued phenotype (C-D). E-H: DIC + GFP images of normal WT wing discs (E) compared to Scrib RNAi (F) show neoplastic overgrowth whereas recused wing discs in Drp1 RNAi ;Scrib RNAi (G) and Hep RNAi ;Scrib RNAi (H) show organized monolayer imaginal discs. Comparison of wing disc area in indicated genotype is shown (I, n=8). GFP-labeled Ptc-Gal4 expression (green) specifically in anterior to posterior region (J) of wing discs (UAS-GFP;Ptc-Gal4) whereas in Scrib RNAi wing discs (UAS-GFP;Ptc-Gal4>UAS- Scrib RNAi ) show spreading of GFP labelled cells (K) and rescued wing discs UAS-GFP;Ptc-Gal4> UAS-Drp1 RNAi ;UAS-Scrib RNAi and UAS-GFP;Ptc- Gal4>UAS- Hep RNAi ;UAS-Scrib RNAi show restoration of GFP expression pattern (L and M) as wild type. Wild type pupae show normal development inside the pupal case (N) while Scrib RNAi leads to 100% pupal death (O). The rescued pupae upon downregulation of Drp1 ( Drp1 RNAi ; Scrib RNAi ) and JNK (Hep RNAi ; Scrib RNAi ) in Scrib RNAi background show complete fly development inside the pupal case (P and Q) as wild type. Histograms (R) represents percent of dead and live/ rescued pupae for indicated genotypes. Loss of Scrib cell polarity regulator strongly promotes metastasis behavior of cancer cells [ 27 ]. To examine the contribution of Drp1 mitochondrial fission protein and involvement of JNK signaling to prevent metastatic behavior in Scrib abrogated cells, we tagged Ptc-Gal4 driver line with GFP. The genetic crossing schemes are provided in supplementary data (Supplementary Information). In the control wing disc, the GFP expression pattern in Ptc-Gal4 is specifically in anterior posterior boundary region ( Fig.1 . J), whereas in Scrib knockdown there is a migration of cancer cells throughout the disc ( Fig.1 . K). In contrast, genetic knockdown of Drp1 and Hep in Scrib RNAi background block cancer cell migration and restore the GFP expression pattern comparable to control wing disc ( Fig.1 .L and M). Taken together, these result indicate that inhibition of Drp1 and hep in background of Scrib RNAi prevents cancer cell migration, clearly indicating the pioneer role of Drp1 and JNK in prevention of metastasis. Moreover, we found that loss of Drp1/Hep function suppresses pupal death upon Scrib loss and adult flies develop inside the pupal case. Scrib knockdown pupae exhibit 100% lethality at mid pupal stage ( Fig.1 O) unlike wild type pupae that show 98% survival ( Fig.1 N). Drp1 knockdown in Scrib RNAi background rescued 63% pupal lethality ( Fig.1 P). However, Hep knockdown rescued 72% pupal lethality ( Fig.1 Q). Histogram reveals percentage of rescue verses pupal death of developing pupae compared to wildtype ( Fig.1 R). Knockdown of Drp1 and JNK signaling in Scrib RNAi background in wing imaginal discs restores to normal fly development and lifespan The rescued flies of UAS-Drp1 RNAi ; UAS-Scrib RNAi and UAS-Hep RNAi ; UAS-Scrib RNAi genotypes ( Fig.2B and C, respectively) showed phenotypically normal morphology, wing development and reproduction comparable to wild type (A). Albeit, a slight difference was found in life span and wing venation pattern. The wildtype flies exhibited a mean lifespan of 84 days, the Drp1 RNAi and Hep RNAi rescued flies showed a mean lifespan of 76 days, and 72 days, respectively. Download figure Open in new tab Figure 2. Representing healthy escaper flies and adult wings venation pattern. Panel A-C show mature healthy flies of Wild type, Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi . Survival assay (D) showed life span of rescued flies (n=50). Examination of wing phenotype in wild type (E) showed normal wing morphology with regular arrangement of ACV, PCV and bristles while, Drp1RNAi; ScribRNAi (F) and HepRNAi; ScribRNAi (G) showed deformed wing vein pattern. Schematic diagram represents wildtype wing showing the location of longitudinal veins (L0-L6) and anterior cross vein (ACV) and posterior cross vein (PCV) (H). Quantification of wing morphology through measuring the length of L2-ACV (I), ACV (J) and PCV (K) between wild type and escaper flies (n=30) showed statistically significant difference in were analyzed by using one-way ANOVA (analysis of variances) followed by Bonferroni multiple comparison test (*p<0.05 **p<0.01 ***p0.005). Drosophila adult wing venation is composed of two different types of veins, longitudinal veins (LV0-LV6) and the cross veins (ACV and PCV-anterior and posterior cross veins) [ 28 ]. According to latest research, Scrib protein regulates PCV in Drosophila wing to maintain epithelial morphogenesis [ 29 , 30 ]. Our findings suggested that upon genetic manipulation in Scrib RNAi , the length of different veins of rescued flies shortened ( Fig. 2E-H ). We report here significant changes in the length of L2-ACV ( Fig. 2I ), length of ACV that bridge L3-L4 ( Fig. 2J ) and PCV that bridge L4-L5 ( Fig. 2K ) upon loss of function of Scrib in combination of knockdown of Drp1 and Hep gene in the wings of rescue flies compared to wildtype. Drp1 inhibitor, Mdivi-1 induces dose dependent inhibition of cancer cell migration A small molecule quinazolinone derivative Drp1 inhibitor , mdivi-1 blocks the mitochondrial division by inhibiting the GTPase activity of the mitochondrial fission regulator, Drp1 [ 31 ]. We firstly show the effect of mdivi-1 ( M itochondrial div ision i nhibitor-1) on the maintenance of apico-basal polarity of cells upon loss of function of Scrib . In order to assess whether the inhibition of Drp1 maintains the cell polarity, we screened a range of low to high concentrations (15µM to 500µM) of mdivi-1 treatment on Scrib knockdown cancer bearing larvae (GFP; Ptc- GAL4>UAS- Scrib RNAi ). Knockdown of Scrib in the wing discs induces disruption of cell shape and polarity, monolayer arrangement and patterning of wing disc leading to three dimensional overgrowth resulting in a deformed, tumorous disc showing a decrease in the wing disc area ( Fig. 3B ). Low doses (15µM and 30µM) of mdivi-1 fed-orally to tumor-bearing larvae do not show any phenotypic change on the tumor size (data not shown), whereas 60µM and 120µM mdivi treatment showed an increase in the area of the disc ( Fig. 3C and D ). However, at 250µM and 500µM concentrations of mdivi, the monolayer epithelial wing imaginal disc morphology is restored ( Fig. 3E and F ). Download figure Open in new tab Fig 3. Effect of different doses of Mdivi-1 treatment on Scrib RNAi cell migration. DIC images showing the untreated control wing imaginal discs (A) in comparison to the deformed, tumorous disc (B), and gradual restoration of normal morphology upon increase in the dosage of Mdivi-1 treatment (C-F). The control wing disc shows GFP expression pattern specifically in the anterior to posterior boundary (G) whereas Scrib RNAi shows the spreading of GFP positive cells (H). Mdivi-1 treated Scrib RNAi larvae (I-L) show gradual restoration of GFP expression pattern. Rhodamine Phalloidin staining of wing discs representing proper F-actin arrangement in WT (M) as compared to disruption of actin in Scrib RNAi wing disc (N), while restoration of actin cytoskeleton and inhibition of neoplastic overgrowth can be seen in mdivi-1 treated wing imaginal discs (O-R). Further, the mdivi-1, was also effective against cancer cell migration and minimized the metastasis in Scrib knockdown wing imaginal disc. In third instar wildtype larvae (GFP; Ptc- GAL4), GFP expression pattern is confined specifically to a single strip in the mid region of the monolayer epithelial wing disc ( Fig. 3G ). This pattern is lost in the Ptc-Gal4 driven Scrib RNAi (GFP; Ptc-GAL4>UAS- Scrib RNAi ), and the GFP-tagged cancer cell migration is found beyond the Ptc expressing domain in the tumorous disc ( Fig. 3H ). Oral administration of low doses (15µM and 30µM) of mdivi-1,does not show alteration in the GFP expression pattern compared to the untreated tumorous wing disc (data not shown). At 60µM, there is an increase in the number of GFP expressing cells, but no change is found in the expression pattern of GFP ( Fig. 3I ). 120µM mdivi concentration shows effective response and partially restores the expression pattern of GFP ( Fig. 3J ). 250µM mdivi-1 furtherinhibited the migration of GFP tagged Scrib RNAi cells ( Fig. 3K ). 500µM of mdivi-1 blocked the migration of GFP-tagged Scrib RNAi cells into the surrounding area and completely restored the GFP expression pattern comparable to wildtype, establishing it to be the effective concentration required for the rescue ( Fig. 3L ). Moreover, we also observed the effect of mdivi-1 on the cytoskeleton arrangement of F-actin counterstained with rhodamine phallodin ( Fig. 3M-S ). The wildtype disc shows a proper arrangement of actin ( Fig. 3M ), while loss of function of Scrib alters the distribution of actin filaments in the tumorous wing disc ( Fig. 3N ). Low doses (15µM and 30µM) of mdivi-1 treatment showed no changes in actin arrangement. However, 60µM and 120µM mdivi-1 treatment partially rescued the arrangement of actin ( Fig. 3O-P ). Further, 250µM mdivi-1 treatment shows significant improvement in the cytoskeleton arrangement of F-actin ( Fig. 3Q ). Mdivi-1 high dose (500µM) restored the proper arrangement of phallodin labeled actin, confirming it to be the effective dose for the rescue of Scrib knockdown tumorous wing disc ( Fig. 3R ). Collectively, the above data revealed that a high dose (500µM) of mdivi-1 treatment decreases cell proliferation, inhibits cell migration, restores F-actin cytoskeleton arrangement and maintains apico-basal cell polarity. We show here that the dose-dependent effect of mdivi-1 to inhibits cell proliferation and cell migration in the tumorous wing disc. Scrib regulates polarity of cells through the Drp1-JNK signaling pathway The downregulation of Scrib activity contributed to elevated Drp1-JNK activation, which leads to exacerbation of the apico-basal cell polarity defects in Drosophila . We and others demonstrated that loss of Scrib function leads to loss of cell polarity with absolute pupal death during early stage of fly pupal development [ 14 ]. Our novel findings suggested that, loss of Drp1 and JNK upstream regulator, Hep gene function in Scrib knockdown background results in restoration of cells polarity and recovery from early death of Drosophila pupae. To explore the molecular mechanistic action of Drp1-JNK signaling in regulation of cell polarity and metastasis upon loss of Scrib , we measured the mRNA expression level using qRT-PCR and protein expression level using immunostaining and western blotting analysis. Immunostaining revealed that Scrib knockdown tumorous disc shows upregulation of Drp1, PJNK and MMP1 expression as compared to wildtype and rescued wing discs ( Fig. 4A-L ). Download figure Open in new tab Fig. 4. Panel shows immunostaining, Real-time PCR and Western blot analysis for Scrib, Drp1, pJNK and MMP1 in Drosophila . A-L Wing disc from Wild type, Scrib RNAi , and Drp1RNAi; Scrib RNAi and Hep RNAi ; Scrib RNAi rescue genotypes showing Drp1 (A-D), pJNK (E-H) and MMP1 (I-L) staining. Wing disc from Scrib RNAi showed significant upregulation of Drp1 (green, B), phospho-JNK (red, F) and MMP1 (red, J) expression as compared to wild type and rescued wing discs ( Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi ). Real-time PCR (M-O, R) and western blot (P and Q) for Drp1, JNK and MMP1 also revealed the upregulation of Drp1, JNK and MMP1 expression in Scrib knockdown as compared to wild type (A) and rescue genotypes. Lane 1, 2, 3 and 4 represents the protein band intensity for Wild type, Scrib RNAi , Drp1RNAi; Scrib RNAi and Hep RNAi ; Scrib RNAi respectively (S) A schematic model showing Scrib inhibits the gene expression of Drp1 and JNK to retained the polarity of the cells and also to regulate cell proliferation, apoptosis and maintenance of ROS levels. (T) PPI network of Drp1 with Scrib, JNK and MMP1. Statistically significant differences were analyzed between wildtype and tested group of indicated genotype by using one-way ANOVA (analysis of variances) followed by Bonferroni multiple comparison test and two-tailed student’s t test for real-time PCR (*p<0.05 **p<0.01 ***p0.005). Further, to validate the immunostaining results, we performed qRT-PCR to measure gene expression level of Drp1, JNK and MMP1 in Scrib knockdown compared to wildtype and rescue pupae. The mRNA transcript level of Drp1 is 2.97 fold upregulate in Scrib knockdown while 0.73 and 0.81 fold downregulated in Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi , respectively ( Fig. 4M ). Further, the mRNA transcript level of JNK is 16.87 fold upregulated in Scrib knockdown while downregulation in rescue pupae shows 0.64 fold in Drp1 RNAi ; Scrib RNAi and 1.37 fold in Hep RNAi ; Scrib RNAi ( Fig. 4N ). Whilst, the mRNA transcript level of MMP1 is 1.99 fold upregulated in Scrib knockdown while 0.16 and 0.50 fold downregulated in Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi in rescue genotype ( Fig. 4O ). No significant difference was found in Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi compared to wildtype. A similar result was found in western blot assays where protein expression of Drp1 ( Fig. 4P ) and MMP1 ( Fig. 4Q ) was upregulated in Scrib knockdown while in the rescue pupae, Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi the protein level is downregulated. Further, to confirm the knockdown efficiency of Ptc-gal4> Scrib RNAi upon co-induction of Drp1 RNAi and Hep RNAi , being driven by the same Gal4, we performed RT-PCR for the Scrib gene. The RT-PCR gene expression analysis revealed that Ptc-Gal4>UAS- Scrib RNAi line could efficiently silence the Scrib gene despite the co-expression of Drp1 RNAi and Hep RNAi transgenes. The mRNA transcript level of Scrib is 0.48 fold downregulated in Scrib knockdown and 0.17 fold and 0.13 fold downregulated the rescue genotypes, Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi , respectively compared to wild type ( Fig. 4R ). The schematic model represents the summary of molecular studies. It shows that Scrib positively regulates the expression of Drp1 and, Hep genes to maintain the polarity of cell and suppresses the cancer cell migration by depletion of MMP1 gene expression ( Fig. 4S ). Using STRING database, a protein-protein interaction (PPI) network diagram was automatically generated for Scrib, Drp1, Hep and MMP1 proteins.The protein Scrib, has an experimental link with JNK upstream regulator, Hep and metastasis marker, MMP1 protein whereas, Drp1 protein, did not show any interaction. The Scrib protein is co-expressed with Hep protein. So, this in-silico study clearly reveals that Drp1 has no previous experimental connection with polarity regulator Scrib protein, metastasis regulator MMP1 protein and JNK upstream regulator, Hep protein. Our results for the first time show the experimental connection between Drp1 with Scrib protein and also with JNK and MMP1protein, to maintain the polarity of the cells ( Fig. 4T ). Knockdown of Drp1 and Hep in Scrib RNAi background reduces ROS production and oxidative stress markers ROS plays a vital role in regulation of neoplastic tumor growth upon loss of Scrib [ 32 ]. Therefore, we examined the effect of Drp1 depletion and JNK inactivation on ROS production upon loss of Scrib . To detect in-vivo intracellular ROS production in Scrib knockdown wing imaginal disc, we used H2DCFDA (2’,7’-dichlorodihydro fluorescein diacetate) staining, a cell membrane permeable indicator used for detection of ROS. In-vivo cellular ROS production in wildtype shows weak signals ( Fig.5A ) whereas in Scrib KD wing imaginal discs show very strong green fluorescent signal suggested excess ROS production in tumor bearing wing imaginal discs ( Fig.5B ). Furthermore, rescued wing imaginal discs Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi show inhibition of ROS production as revealed by faint green fluorescent signal as compared to wildtype ( Fig.5C and 5D). Download figure Open in new tab Fig 5. H2DCFDA staining and biochemical assays to detect in situ ROS and oxidative stress. In situ ROS detection using 2,7dichlorodihydrofluorescein diacetate (H2DCFDA) staining (green), and imaging by the fluorescence microscope (A-D). The wild type (A) wing discs show low level in situ cellular ROS as compared to tumorous wing imaginal discs (B) while in rescued wing discs ( Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi ) ROS production is comparable to control (C and D). The Intensity line graphs showed total ROS production in wild type, Scrib RNAi and rescued wing discs along the white arrow drawn by NIS-Elements BR 4.3 software. SOD activity (E), H2O2 production (F), Catalase activity (G) and TBARS level (H) measured in Scrib RNAi and rescued group compare to wild type. The statistical significance was analyzed using one-way ANOVA (analysis of variances) followed by Bonferroni multiple comparison test (*p<0.05 **p<0.01 ***p0.005). Further, biochemical assays such as SOD (superoxide dismutase) activity assay, H2O2 (hydrogen peroxide) production assay, Catalase activity assay and LPO (lipid peroxidation) assay, were also performed for detection of ROS production. In tumor cells, elevated mitochondrial ROS production is altered by aberrant expression of antioxidant enzymes, like superoxide dismutase, catalase and excess production of lipid peroxidation (LPO) and hydrogen peroxide (H2O2), a characteristic marker for oxidative stress [ 33 ]. We determined the activities of antioxidant enzymes in wildtype compared to Scrib knockdown and genetic rescue pupae to check the status of oxidative stress (OS). The Scrib KD showed 2.49-fold higher SOD activity while the rescued genotypes Drp1 RNAi ; Scrib RNAi and Hep RNAi ; Scrib RNAi showed reduced SOD activity,1.04 and 0.95 fold respectively, almost comparable ( Fig.5E ). Superoxide dismutase, catalyzes the superoxide radical to hydrogen peroxide, H2O2. So, we further evaluated the status of H2O2 production. Scrib RNAi , displayed significant upregulation of H2O2 compared to wildtype and rescue pupae ( Fig.5F ). The excess H2O2 production is further catalyzed by antioxidant catalase enzymes, which converts H2O2 to water and molecular oxygen [ 34 ]. Scrib RNAi pupae displayed reduced catalase activity compare to wildtype and rescued pupae ( Fig.5G ). Moreover, to determine the effect of excessive ROS production in the context of lipid peroxidation, TBARS assays was performed. The pink color end product was measured using multimode plate reader at absorbance of 532nm and 600nm [ 35 ]. The lipid peroxidation marker, TBRAS level shows significant elevation in Scrib KD cells whereas, in rescued pupae it showed reduction of LPO in form of reduce in TBRAS levels as compare to wildtype ( Fig.5H ). Knockdown of Drp1 and JNK signaling in loss of Scrib RNAi regulates the balance between cell proliferation and apoptosis to maintain the mitochondrial morphology To investigate the role of Drp1 in maintenance of apico-basal cell polarity by regulating the Drp1- mediated mitochondrial fission in Scrib knockdown cells, we examined mitochondrial distribution in control, Scrib knockdown, Drp-1 knockdown and JNK knockdown under Scrib knockdown backgrounds in wing imaginal discs. We used an outer mitochondrial membrane tagged mitocherrythat showed healthy and defective mitochondria in wildtype and Scrib RNAi respectively ( Fig. 6A and B ). Scrib knockdown wing disc contained severely defective, aggregated, fragmented and randomly distributed mitochondrial throughout the tumorous wing disc, unlike the wild type where an orderly mitochondrial arrangement is seen across anterior to posterior region of wing discs. Strikingly, when Drp1 and Hep were knockdown in Scrib RNAi cells, UAS-Drp1 RNAi ; UAS-Scrib RNAi and UAS-Hep RNAi ; UAS-Scrib RNAi , the mitochondrial structure and arrangement was restored as wildtype, specifically in anterior posterior boundary region ( Fig.6 .C and D; Cross scheme provided in supplementary data). The magnified images are shown in Fig.6 A’-D’. Download figure Open in new tab Fig 6. Confocal images representing mitochondrial distribution (A-D’), anti-Ser10 phosphorylated histone (PH3) staining (E-H) and Tunel assay (J-M) in wing imaginal discs. (A, A’) Wild type discs (genotype mitocherry Ptc-Gal4;+) showed well-arranged mitochondria, specifically in anterior and posterior region of wing imaginal discs whereas Scrib RNAi discs showed distorted and aggregated arrangement of mitochondria throughout the tumorous wing discs (B, B’). The rescued wing discs (C, C’ and D, D’) showed similar arrangement as Wildtype. The magnified confocal projection showed rescued flies wing imaginal disc shows maintenance of similar mitochondrial morphology as wild type compared with Scrib RNAi tumorous discs ( Fig.5 .A’ and D’). Anti-PH3 staining in wild type (E) wing imaginal discs showed less PH3 positive cells across anterior to posterior boundary whereas in Scrib RNAi cells (F) shows overlap and aggregate PH3 stained + cells while in rescued wing discs shows less PH3 positive cells in A/P boundary region of wing imaginal discs (G and H). A, indicate anterior region and P, indicate posterior region of Drosophila wing imaginal disc. The histogram shows the significant increasein PH3 positive cells in tumor disc as compare to wildtype and rescued wing disc (I). Panel (J-M) shows tunel assay of wild type (J) and Scrib RNAi wing imaginal discs (K). Number of tunel positive cell (red) are greater in Scrib RNAi cells compared to wild type while the rescued wing imaginal discs (Drp-1 RNAi and Hep RNAi ) show less tunel positive cells (L and M). Histogram showing the reduction of ATP levels in Scrib RNAi while significant restoration of ATP levelswere observed in rescued pupae as compared to Scrib RNAi pupae (N). Earlier well documented studies show that loss of Scrib promotes excessive cell proliferation and evades apoptosis [ 26 , 36 ]. Here, our results show that upon loss of Drp1 and hep in background of Scrib RNAi , control the cell proliferation and regulates the apoptosis as revealed by PH3 staining and tunel assays respectively. The abnormal and uncontrolled growth of Scrib knockdown cells induces excessive cell proliferation. Therefore, we firstly assess the cell proliferation status across the anterior to posterior boundary upon knockdown of Drp1 in Scrib RNAi background by assessing expression of Phospho-histone PH3 staining, marker for proliferating cells (indicated by red color) ( Fig. 6 .E-H). In the wildtype wing imaginal discs, very few PH3 positive cells were observed in anterior-posterior (A/P) boundary region of the discs ( Fig.6 .E). However, in tumorous disc larger number of PH3 positive cells enrichedaround apparent A/P region which is encircled in red and spread all over the wing disc ( Fig.6 .F) while lesser number of PH3 positive cells present in A/P boundary region of rescued wing imaginal disc upon depletion of Drp1 and JNK inactivation in background of Scrib knockdown ( Fig.6 .G and H). Further, quantification of PH3 positive cells also shows significant larger number of proliferating cells in Scrib RNAi as compared to wildtype while rescued wing imaginal discs showed less number of PH3 positive cells ( Fig.6 .I). These results suggested that depletion of Drp1 positively regulates the over proliferation of the cancer cells upon knockdown of Scrib . Similarly, upon knockdown of JNKupstream regulator, Hep gene also positively control the cell proliferation in background of Scrib RNAi for maintaining the apico-basal polarity of the cells. In cancer, there is a competition between cell proliferation and apoptosis which plays major role in survival of cancer cells [ 37 ]. So, to determine whether loss of cell polarity upon knockdown of Scrib induces apoptosis, we examined third instar larval wing imaginal discs which are subjected to the tunel assay for marking the apoptotic cells. There is a striking increase in the number of apoptotic cells in tumorous wing disc as compared to wildtype ( Fig.6 .J and K). Furthermore, the knockdown of Drp1 and Hep gene in background of Scrib RNAi shows decrease in number of tunel positive cells in rescued wing imaginal discs ( Fig.6 .L and M), suggesting that Drp1-mediated mitochondrial fission contributes in apoptosis regulation upon loss of Scrib gene to maintain the cell polarity. Mitochondria are double membrane bound dynamic cell organelles that are power house for energy production and also prime source for ROS production [ 38 ]. In several diseases, the overproduction of ROS is associated with a decrease in ATP synthesis due to mitochondrial dysfunction which fail to maintain cellular energy [ 39 ]. Therefore, it is important to measure the ATP levels upon Drp1 depletion in background of loss of Scrib . Notably, our results clearly show that unlike wild type, overproduction of ROS leads to significantly reduced the ATP production in Scrib knockdown suggesting mitochondria dysfunction ( Fig.6 .N). Furthermore, the depletion of Drp1 and Hep significantly restores the ATP production ( Fig.6 .N). Discussion Drosophila third instar larval wing imaginal discs are monolayered epithelial cells that develop and differentiate into adult appendages, the wings, and most frequently used for modeling cancer [ 8 ], including our current study. Scrib , the apico-basal polarity regulator gene of epithelial cells is necessary for maintenance of epithelial homeostasis [ 40 ]. According to earlier research findings, the Scrib, a lateral membrane associated protein found at cell-cell junction (adherens junction-AJs). Scrib is mislocalized to the cytoplasm of cancer cells upon loss of function mutation in Scrib complex proteins [ 41 – 43 ]. The loss of function mutation of Scrib leads to development of neoplastic tumor [ 27 , 44 ]. Earlier reports from our laboratory showed larval wing tissue specific knockdown of Scrib developed neoplastic tumors with increased larval size and die as giant pupae during early stages of pupal development without differentiation [ 14 ]. The tumorous wing imaginal discs have three dimensional multilayered proliferation of cells rather than highly organized monolayered flattened epithelial cells as in wild type discs. The molecular mechanisms of polarity regulation upon loss of Scrib remains elusive. In recent years, the role of mitochondrial-dynamics regulators has been well investigated in cancer progression and metastasis [ 14 , 45 ]. Cancer cells show differential expression of mitochondrial dynamics regulator genes leading to enhanced fission-fusion events, causing disruption of mitochondrial morphology. The fusion-fission events are critical for maintenance of mitochondrial dynamics and polarity aspects of cells. The mitochondrial fission and fusion events are critically affected in several diseases including cancer and metastasis conditions. The Drp1 is emerging as key regulator of fission events and JNK is also emerging as key component in Drp1 mediated regulation of mitochondrial dynamics. Although the role of mitochondria in several types of cancer and neurodegenerative diseases have been well investigated, but the role of Drp1-JNK mediated mitochondrial fission event in regulation of cell polarity maintenance remains unexplored. To further explore the molecular mechanistic action of Scrib on mitochondria dynamics, the double transgenic fly lines ( UAS-Drp1 RNAi ; UAS-Scrib RNAi and UAS-Hep RNAi ; UAS-Scrib RNAi ) were created to investigate the role of Drp1 and JNK in regulation of cancer progression and metastasis. To test our hypothesis that the upregulation Drp1 in Scrib knockdown tumorous tissues is responsible for neoplastic tumor development, we genetically downregulated Drp1 expression levels using UAS-Drp1 RNAi , in the background of UAS-Scrib RNAi <Ptc-Gal4 (referred as a UAS-Drp1 RNAi ; UAS-Scrib RNAi ), which resulted in significant reduction in tumor growth and complete rescue leading to development of healthy Drosophila flies. The same phenotype was also observed by downregulation of JNK upstream regulator, Hep, in the background of UAS- Scrib RNAi <Ptc-Gal4 (referred as a UAS-Hep RNAi ; UAS-Scrib RNAi ). We genetically modulated Drp1/Hep expression in Scrib knockdown background using RNAi approach and show the rescue of cancer phenotypes, resulting in healthy adult escaper flies ( Fig. 1 ). We further show for the first time that the Drp1 depletion in Scrib knockdown cells rescue from extended larval development and larval overgrowth phenotypes. To further confirm the effect of Drp1 RNAi and Hep RNAi in cancer bearing larvae, we allowed third instar larvae to mature into adult flies and examined the morphological alteration, if any, and reproductive aspects compared to wild type counterparts. The escaper flies ( UAS-Drp1 RNAi ; UAS- Scrib RNAi and UAS-Hep RNAi ; UAS-Scrib RNAi ) were healthy except exhibiting a minor differences in wing venation pattern of wing but the reproduction and lifespan were similar to wild type ( Fig. 2 ). The phenotypic, biochemical and molecular changes upon knockdown of Drp1 expression encourages us to exploit the chemical approach and examine the effect of mdivi-1, a Drp1 inhibitor on the Drp1 mediated mitochondrial division. For this, we assessed the dose dependent effect of mdivi-1 on cancer bearing scrib knockdown larvae. The mechanistic action of mdivi-1 on the GTPase activity of Drp1 suggest that it inhibits the mitochondrial fission and positively regulates the polarity of scrib knockdown cells. We observed the restoration of wing imaginal disc morphology by mdivi-1 to maximum extent but it could not rescue from pupal lethality phenotype. The fact that mdivi-1 restores wing disc morphology of Scrib RNAi suggests Drp-1role in cancer progression and metastasis. Mdivi-1 could not rescue from metastasis at pupal development. Inhibition of Drp1 mediated restoration of mitochondrial fission is considered as an efficacious therapeutic targets for the prevention of cancer. Further studies are required to evaluate how exactly mdivi-1 and Drp-1 play role in regulation of mitochondrial division and regulating the cell polarity in the absence of Scibble ( Fig. 3 ). Our molecular studies suggest that Scrib regulates polarity of cells through the JNK-Drp1 mediated mitochondrial fission event. The downregulation of Scrib contributed to Drp1 activation, which led to migration of Drp1 from the cytoplasm onto the surface of the mitochondria, indicative of mitochondrial fission activation. Earlier reports from our laboratory clearly showed that the Scrib RNAi knockdown leads to upregulation of Drp1 which was rescued through HepRNAi [ 14 ]. In order to check whether Drp-1 downregulation under Scrib RNAi knockdown background can rescue the loss of cell polarity and pupal lethal phenotype, we further genetically modified the expression of Drp1 in Scrib RNAi background using Drp1 RNAi lines, which restored cell polarity as evident by the restoration of wing discs morphology and eventually rescued the pupal death phenotype. Wevalidated the downregulation of Drp1 by Immunostaining, qRT-PCR, western blotting analysis. Although it is clear that Drp1 regulate JNK pathway [ 46 , 47 ] but its role in Scrib mediated neoplastic tumor development is completely unknown. We went onto show that Drp1 is also key target of JNK downstream regulator, Hep gene that contributes to neoplastic tumor growth. To test this hypothesis, we genetically knocked-down the Hep gene using Hep RNAi line in Scrib RNAi background. The loss of Drp1 - mediated fission event downregulates the JNK expression while loss of Hep gene downregulates the Drp1 expression in Scrib knockdown cells. Immunostaining, RT-PCR and western blotting data revealed that genetic manipulation using RNAi lines leads to silencing of the Drp1/Hep gene expression in Scrib RNAi background. Overall, our results for the first time provide evidence that Drp1 and JNK activation leads to tumor progression and Drp1-JNK inhibition by genetic and chemical inhibition means reverse cancer progression and metastasis as a downstream mechanism of Scrib knockdown cells gives new insight into the mechanistic action of Scrib in cancer cells. In summary, it is concluded from these studies that reduced Drp1 - JNK mediated fission events in tumor cells help maintain the apico-basal polarity of the cells. Drp1 knockdown inhibits mitochondrial fission event and maintain mitochondrial homeostasis in Scrib knockdown cells. Moreover, down regulation of Drp1/Hep genes under Scrib RNAi background can also suppress malignant behavior of metastatic secondary tumors which is a major causative event of cancer related deaths [ 48 ]. The elevated levels of MMP1 expression during metastasis window period and its rescue by down regulation of MMP1 were evaluated by using qRT-PCR for RNA expression, immunostaining for in situ MMP1 localization and western bolt analysis for protein quantification clearly demonstrated that the downregulation of Drp1 and JNK play role in altering MMP1 to restore normalcy. Our data clear shows, Scrib regulates mitochondrial fission via the JNK-Drp1 mediated pathways to control cancer progression and metastasis. This novel involvement of Scrib-JNK-Drp1 pathway in regulating the cell proliferation, maintaining the polarity of cells through modulating the dynamics of mitochondria in cancer cells can be considered as strategic therapeutic target for several advance stage cancers ( Fig. 4 ). Several types of human cancer have been reported to be associated with mitochondrial dysfunction and ROS production [ 47 ]. Mitochondrial dysfunction is a prime source of overproduction of ROS resulting in oxidative stress [ 49 ]. The imbalance between ROS production and impaired enzymatic activity of antioxidant agents leads to mitochondrial dysfunction in cancer. Scrib knockdown elevated activity of SOD, H2O2 and LPO, reduced catalase activity while Drp1 and JNK downregulation under Scrib knockdown background showed reduced activity of ROS. The reduced oxidative stress by Drp1/JNK downregulation in Scrib knockdown background indicate the role of Drp1 and JNK in activating the oxidative stress while Scribble counteract Drp-1 and JNK to maintains the oxidative parameters and polarity of the cells in an optimal manner ( Fig. 5 ). It is intriguing to note that downregulation of Drp-1 and JNK restoring the cell polarity and rescuing lethality phenotype despite loss of Scribble could be possibly due to blocking mitochondrial fission events, which require further studies. Overall, Scrib plays a role in maintaining the cell polarity and cell proliferation regulation by blocking the Drp1 and JNK which regulate mitochondrial dynamics, via maintaining the mitochondrial membrane polarity, morphology and oxidative stress. Mitochondria, a dynamic double membrane-bound cell organelles, play crucial role in ATP production, recently emerged as hub for Drp-1 mediated mitochondrial fission protein interactome connective pathways for various diseases [ 50 ]. Our results clearly show that upon knockdown of Scrib leads to enhanced Drp-1 mediated disruption of mitochondrial dynamics, which induces enhanced ROS production and cause reduced ATP production leading to increased cell proliferation and metastasis. On other hand, Drp1 and JNK downregulation together with Scrib RNAi suppress ROS production, enhances ATP production, maintains cell polarity and restores normal fly development. These findings suggest that mitochondrial fission regulator, Drp1is key modulator of altered mitochondrial dynamics upon loss of Scrib through altering the ROS production, ATP generation, excess cell proliferation and metastasis. It is well documented that Drp-1 plays is a central role in triggering the mitochondrial fission mediated metastasis and apoptosis [ 51 ]. Our findings also show Drp1 and JNK depletion enhance cell death phenomena in Scrib abrogated cells thereby restoring normal development. More has to be probed into the mechanistic aspects of Drp-1-JNK-Scrib related cell regulation in normal development versus cancer progression and metastasis. Conclusions The Scrib-JNK-Drp1 pathway play unique role in regulating planer cell polarity (PCP) of epithelial cells. Dysfunction of above pathway is observed in loss of Scrib . Our findings demonstrated for the first time that Drp1 -knockdown and mdivi treatment markedly suppressed excessive cell proliferation and regulates the apico-basal cell polarity loss induced by knockdown of the Scrib . We also found that Hep inactivation attenuates the Drp1 mediated mitochondrial dysfunction and regulate the PCP in the Scrib knockdown induced tumor cells. The alteration in mitochondrial fission regulator, Drp1 provide important clues towards prevention and therapeutic intervention for the cancer drug discovery. Conflict of interest None Supplementary Information View this table: View inline View popup Acknowledgments This work was supported by a research grant (P07/598) awarded to SS by the Science and Engineering Research Board, India and ICMR-Senior Research Fellowship (ICMR-SRF) to JS. Authors are highly grateful to IoE (Institution of Eminence) for financial support under incentive grant and SATHI for central facility. The authors would like to acknowledge ISLS-BHU and for Confocal Microscopy, Nanodrop, Real time PCR and other routine facilities. References 1. ↵ Kapil S , Sharma BK , Patil M , Elattar S , Yuan J , Hou SX , Kolhe R , Satyanarayana A . The cell polarity protein Scrib functions as a tumor suppressor in liver cancer . Oncotarget . 2017 Apr 4; 8 ( 16 ): 26515 . 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Share Drp1-JNK knockdown mitigates Scribble loss induced cell proliferation, metastasis and lethality phenotypes in Drosophila Jyotsna Singh , Saripella Srikrishna bioRxiv 2024.04.10.588977; doi: https://doi.org/10.1101/2024.04.10.588977 Share This Article: Copy Citation Tools Drp1-JNK knockdown mitigates Scribble loss induced cell proliferation, metastasis and lethality phenotypes in Drosophila Jyotsna Singh , Saripella Srikrishna bioRxiv 2024.04.10.588977; doi: https://doi.org/10.1101/2024.04.10.588977 Citation Manager Formats BibTeX Bookends EasyBib EndNote (tagged) EndNote 8 (xml) Medlars Mendeley Papers RefWorks Tagged Ref Manager RIS Zotero Tweet Widget Facebook Like Google Plus One Subject Area Cancer Biology Subject Areas All Articles Animal Behavior and Cognition (8025) Biochemistry (18805) Bioengineering (14932) Bioinformatics (44522) Biophysics (22648) Cancer Biology (19785) Cell Biology (26964) Clinical Trials (138) Developmental Biology (14000) Ecology (21057) Epidemiology (2067) Evolutionary Biology (25503) Genetics (16189) Genomics (23542) Immunology (18738) Microbiology (42587) Molecular Biology (18116) Neuroscience (93667) Paleontology (701) Pathology (2989) Pharmacology and Toxicology (5106) Physiology (8134) Plant Biology (16035) Scientific Communication and Education (2098) Synthetic Biology (4575) Systems Biology (10258) Zoology (2393) window.__CF$cv$params={r:'a403e2f7b8667885',t:'MTc5MDI3NDkwMQ==',u:'01a0d4b31fb670b2880a3c9ca6259b95',ut:'deozxqNInsEc5Y1EqeK5ueRvzJcr5MahBuZmY4gH3oU-1790274903-1.2.1.1-j3cXpcNX1Ml4y4rOdI_WQ1kwvszRNcLpUQtde6UaKYDhcd9wcNpfgv3D2sPRV.RUjTlhSW.7YGcnxZmxaLMbotQpA2qmCkSX2Mt2U0izpNM',i:60};(function(){if(!document.body)return;var s=document.createElement('script');s.src='/cdn-cgi/challenge-platform/scripts/precursor/main.js';document.head.appendChild(s);})();