Summary: Chemical Modulation of Circadian Rhythms and Assessment of Cellular Behavior via Indirubin and Derivatives

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Abstract

We present a summary of our protocol for employment of small-molecule circadian rhythm modulators. The abilities of compounds to affect oscillations have received significant attention. We outline assessments of circadian changes using indirubin-3’-oxime (IO), 5-iodo-indirubin-3’-oxime (5I-IO), and 5-sulfonic acid-indirubin-3’-oxime (5SA-IO), analyzing effects on Bmal1 and Per2 oscillations and oncogenic features.

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europepmc
last seen: 2026-05-19T01:45:01.086888+00:00