Comparison of different effective parts of Panax Ginseng in enhancing immune function of mice

The present study focused on comparing the effects of different active parts of five-year-old ginseng on the immunological activity of cyclo-phosphamide (CTX)-induced immunosuppressed mice. Preparation of ginseng water extract, total ginsenoside complex, ginseng glycoprotein complex, ginseng glycopeptide complex, by water extraction and alcohol precipitation method and enzymolysis method. The immunomodulatory effects of different effective parts of ginseng were determined by carbon clearance test, immune organ index, delayed type hypersensitivity (DTH) response, leukocyte count in peripheral blood, spleen lymphocyte transformation experiment, splenic NK cell activity, serum hemolysin and Experiment on the determination of cytokines. The results showed that ginseng water extract, total ginsenoside complex, ginseng glycoprotein complex and ginseng glycopeptide complex improved immune organs indices, DTH response, leukocyte count, NK cell activity, cytokine content, hemolysin content and the phagocytosis of macrophages in immunosuppressive mice. Ginseng glycoprotein complex and ginseng glycopeptide complex had more effectiveness on cellular immunity. Total ginsenoside complex and ginseng glycoprotein complex can better enhance the humoral immune function of mice. Total ginsenoside complex, ginseng glycoprotein complex and ginseng glycopeptide complex had a better effect on improving the function of macrophages phagocytosis. The enhancing effect of ginseng glycoprotein on NK cell activity was better than other extracts. Total ginsenoside complex, ginseng glycoprotein complex and ginseng glycopeptide complex can significantly increase the content of TNF- α in mice, ginseng water extract can significantly in-crease the content of IL-2, ginseng water extract and total ginsenoside complex are more effective in enhancing IL-6 levels in mice. Different effective parts of ginseng can improve the immunity of mice, and the effect of ginseng glycoprotein is more obvious. It is speculated that ginseng glycoprotein is the main active site of ginseng against CTX immunosuppression. This study laid a theoretical foundation for further study on the pharmacodynamic material basis and mechanism of ginseng to improve immunity.