RE07 : Effect of particulate matter on endometriosis

Objective: Endometriosis is a common gynecologic disorder that is characterized by chronic pelvic pain, dysmenorrhea, and infertility. Although the adverse effects of particulate matter on the human body and many diseases are proven, studies of the effects on endometriosis and related infertility are insignificant not only in Korea but around the world. The object of this study is to evaluate the effect of the particulate matter on the endometrium of the endometriosis compared to controls. Methods: During the surgery, endometrial tissues in patients with and without endometriosis were obtained under informed consent. Benign ovarian cysts without endometriosis were served as controls. Each endometrium of the endometriosis and control cells were treated with 400ug of the urban particulate matter(SRM 1648a). Cell Counting Kit-8 (CCK-8) was used to determinate the cell viability. After protein extractions, expression levels of four cell signaling molecules related to apoptosis, caspase3, BAX, Bcl-2, and p53 were evaluated by using western blot. Results: After treating the particulate matter, cell viability was decreased in endometrium of both endometriosis and controls, with significanlty higher decrease rate in endometriosis group. Also, Expression of Caspase3, BAX, p53 was lower in eutopic endometrium of endometriosis compared to controls while Bcl-2 was higher in endometriosis. When particulate matter was applied, all protein levels in eutopic endometrium of the controls were decreased, while the decrease tendency was not definite in the endometrium of the endometriosis group. Conclusion: Particulate matter affects the cell proliferation and apoptosis of the endometrium cells by altering the cell signaling molecules. The effect of particulate matter was different in endometriosis group compared with control group and endometriosis might have reseistance in particulate matter.