Doubled haploidy methodology for three forage grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Bromus riparius x B. inermis), and meadow bromegrass (Bromus riparius Rehm.)]

Doubled haploidy methodology for three forage grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Bromus riparius x B. inermis), and meadow bromegrass (Bromus riparius Rehm.)] | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Doubled haploidy methodology for three forage grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Bromus riparius x B. inermis), and meadow bromegrass (Bromus riparius Rehm.)] Alison M.R. Ferrie, Kim Nelson, Bill Biligetu This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3917360/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 02 Mar, 2024 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted 4 You are reading this latest preprint version Abstract Doubled haploidy (DH) methodology is used in many plant species to accelerate crop improvement and cultivar development; however not all species are amenable to the tissue culture technique. Experiments were undertaken to develop DH protocols for three perennial grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Bromus riparius x B. inermis), and meadow bromegrass (Bromus riparius Rehm.)]. The initial experiment screened these forage grass species to established wheat (Triticum aestivum L.) microspore culture protocols. Following the initial screen, several factors influencing microspore embryogenesis were evaluated. These included genotype, donor plant conditions, developmental stage of the microspore, pretreatments, media composition, and culture conditions. For regeneration of the embryos to plants, media composition and culture conditions were assessed. Microspore-derived embryos/calli as well as green haploid/doubled haploid plants were regenerated from all three forage grasses. Differences were observed between species and genotypes within species in terms of embryogenic response. Modifications to the initial wheat DH protocol included the donor plant conditions, developmental stage of the microspore to late uninucleate to early binucleate and media composition. Regenerated plants were grown in the greenhouse. androgenesis crested wheatgrass doubled haploidy hybrid bromegrass meadow bromegrass microspore culture Full Text Cite Share Download PDF Status: Published Journal Publication published 02 Mar, 2024 Read the published version in Plant Cell, Tissue and Organ Culture (PCTOC) → Version 1 posted Reviewers agreed at journal 06 Feb, 2024 Reviewers invited by journal 01 Feb, 2024 Editor assigned by journal 31 Jan, 2024 First submitted to journal 30 Jan, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3917360","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":270455204,"identity":"fbbec9d7-8231-47a8-9906-74d93b6994b6","order_by":0,"name":"Alison M.R. Ferrie","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABEUlEQVRIie3QsUrEMBjA8S8E4tIja8INvkJEOBWO66tEbnB0vaFopJBbDl0tiI9xOPYI1KXuDi5H4SaHFpdbhEt6oIMp1E0k/6EJoT++EIBQ6A9GDpBy67X75jDrQSjeEwktKXsQnu7XlgDSPYgwOK2bJ5B0+LLOm8e3S6rwppolMLlTeF17CdI8K+GK316IVbbcnN3n5OSoLGBqN8esg+CBBilKAmawNEIcvhN+o2AqIAIfiQ1KPxyJHfl8sASiL4K3/ilq2E6JLEHqm0zcputiPNNMMjtltSiMYDkZcVXYE0NGpz7yPK/qRo8lXRBcbxMj3ItxlYxjOk+r1+63/nkBdq5w9//+4t+CUCgU+rftALe+WvN9FueOAAAAAElFTkSuQmCC","orcid":"https://orcid.org/0000-0002-1597-142X","institution":"National Research Council Canada","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Alison","middleName":"M.R.","lastName":"Ferrie","suffix":""},{"id":270455205,"identity":"6c4ec460-ef52-4b1c-af32-5406fc16e644","order_by":1,"name":"Kim Nelson","email":"","orcid":"","institution":"National Research Council Canada","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Kim","middleName":"","lastName":"Nelson","suffix":""},{"id":270455206,"identity":"1ea4aed2-9672-4d7c-94d3-799fcac51d31","order_by":2,"name":"Bill Biligetu","email":"","orcid":"","institution":"University of Saskatchewan","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Bill","middleName":"","lastName":"Biligetu","suffix":""}],"badges":[],"createdAt":"2024-02-01 12:33:34","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3917360/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3917360/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s11240-024-02718-4","type":"published","date":"2024-03-02T15:01:25+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":51958287,"identity":"396a4621-caad-4e32-a14e-b166b509fd76","added_by":"auto","created_at":"2024-03-04 15:15:01","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":815328,"visible":true,"origin":"","legend":"","description":"","filename":"ForageDHJan302024.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3917360/v1_covered_c86fc30f-31c3-4f79-b214-2d9347eeed4c.pdf"}],"financialInterests":"","formattedTitle":"Doubled haploidy methodology for three forage grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Bromus riparius x B. inermis), and meadow bromegrass (Bromus riparius Rehm.)]","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"plant-cell-tissue-and-organ-culture-pctoc","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pcto","sideBox":"Learn more about [Plant Cell, Tissue and Organ Culture (PCTOC)](https://www.springer.com/journal/11240)","snPcode":"11240","submissionUrl":"https://submission.nature.com/new-submission/11240/3","title":"Plant Cell, Tissue and Organ Culture (PCTOC)","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"androgenesis, crested wheatgrass, doubled haploidy, hybrid bromegrass, meadow bromegrass, microspore culture","lastPublishedDoi":"10.21203/rs.3.rs-3917360/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3917360/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Doubled haploidy (DH) methodology is used in many plant species to accelerate crop improvement and cultivar development; however not all species are amenable to the tissue culture technique. Experiments were undertaken to develop DH protocols for three perennial grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Bromus riparius x B. inermis), and meadow bromegrass (Bromus riparius Rehm.)]. The initial experiment screened these forage grass species to established wheat (Triticum aestivum L.) microspore culture protocols. Following the initial screen, several factors influencing microspore embryogenesis were evaluated. These included genotype, donor plant conditions, developmental stage of the microspore, pretreatments, media composition, and culture conditions. For regeneration of the embryos to plants, media composition and culture conditions were assessed. Microspore-derived embryos/calli as well as green haploid/doubled haploid plants were regenerated from all three forage grasses. Differences were observed between species and genotypes within species in terms of embryogenic response. Modifications to the initial wheat DH protocol included the donor plant conditions, developmental stage of the microspore to late uninucleate to early binucleate and media composition. Regenerated plants were grown in the greenhouse.","manuscriptTitle":"Doubled haploidy methodology for three forage grasses [crested wheatgrass (Agropyron cristatum (L.) Gaertn.), hybrid bromegrass (Bromus riparius x B. inermis), and meadow bromegrass (Bromus riparius Rehm.)]","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-02-06 18:53:49","doi":"10.21203/rs.3.rs-3917360/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"reviewerAgreed","content":"","date":"2024-02-06T08:40:47+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-02-01T11:42:03+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-01-31T08:45:18+00:00","index":"","fulltext":""},{"type":"submitted","content":"Plant Cell, Tissue and Organ Culture (PCTOC)","date":"2024-01-30T12:09:43+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"plant-cell-tissue-and-organ-culture-pctoc","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"pcto","sideBox":"Learn more about [Plant Cell, Tissue and Organ Culture (PCTOC)](https://www.springer.com/journal/11240)","snPcode":"11240","submissionUrl":"https://submission.nature.com/new-submission/11240/3","title":"Plant Cell, Tissue and Organ Culture (PCTOC)","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"db23a029-84b3-452f-aa37-43eec22aed05","owner":[],"postedDate":"February 6th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-03-04T15:06:59+00:00","versionOfRecord":{"articleIdentity":"rs-3917360","link":"https://doi.org/10.1007/s11240-024-02718-4","journal":{"identity":"plant-cell-tissue-and-organ-culture-pctoc","isVorOnly":false,"title":"Plant Cell, Tissue and Organ Culture (PCTOC)"},"publishedOn":"2024-03-02 15:01:25","publishedOnDateReadable":"March 2nd, 2024"},"versionCreatedAt":"2024-02-06 18:53:49","video":"","vorDoi":"10.1007/s11240-024-02718-4","vorDoiUrl":"https://doi.org/10.1007/s11240-024-02718-4","workflowStages":[]},"version":"v1","identity":"rs-3917360","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3917360","identity":"rs-3917360","version":["v1"]},"buildId":"GqpaHPwrfC8PjnIFayRh5","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}