Expression and significance of Ki-67 mRNA and caspase-3 mRNA in endometrial glandular epithelial cells of adenomyosis cultured in vitro

Objective To explore the proliferation and apoptosis of the endometrial glandular epithelial cells from adenomyosis and uterine leiomyoma cultured in vitro. Methods The expression of Caspase-3 mRNA and Ki-67 mRNA in the endometrial glandular epithelial cells from patients with adenomyosis(adenomyosis group)and uterine leiomyoma(control group)was detected by semiquantitative RT-PCR. Results The expression of Ki-67 mRNA of the glandular epithelial cells in adenomyosis group was higher than in control group(P0.05,P0.001).In proliferative phase,the expression of Caspase-3 mRNA of the glandular epithelial cells in adenomyosis group was lower than in control group(P0.01).But in the secretory phase,the expression of Caspase-3 mRNA of the glandular epithelial cell showed no significant difference between 2 groups. Conclusion The unbalance of apoptosis and proliferation in the endometrial glandular epithelial cell may be one of pathogenesis of adenomyosis.