OpenMebius2: GUI-based software for 13 C-metabolic flux analysis with tracer labeling pattern suggestions for accurate flux predictions

Summary 13 C-metabolic flux analysis ( 13 C-MFA) is a crucial technique that experimentally determines metabolic flux distribution. Although precision of each flux strongly depends on tracer labeling pattern, its optimization remains challenging. We developed an integrated platform, OpenMebius2, a graphical user interface (GUI)-based software for 13 C-MFA that includes a tracer labeling pattern suggestion function to support subsequent experiments. The proposed function leverages metabolic flux distributions and their 95 % confidence intervals obtained using low-cost 13 C-labeled substrates to evaluate hypothetical parallel labeling scenarios and predict improvements in flux estimation precision. Availability and implementation This software runs on Linux, macOS, and Windows. The source code and binary files are available at https://github.com/metabolic-engineering/OpenMebius2 under the PolyForm Noncommercial License 1.0.0.