Change of dendritic cells in endometrial tissue of endometriosis and its correlation with protease expression and immune response

Objective: To study the change of dendritic cells (DCs) in endometrial tissue of endometriosis \n(EMs) and its correlation with protease expression and immune response. Methods: The \npatients with endometriosis who underwent surgical resection in Xining First People's Hospital \nbetween March 2015 and December 2017 were selected as the EMs group of the research to \ncollected the ectopic endometrial lesion tissues, the patients who underwent total hysterectomy \ndue to benign diseases in Xining First People's Hospital during the same period were selected \nas the control group to collected the eutopic endometrial lesion tissues, and the number of DCs, \nthe contents of proteases and cytokines as well as the expression of transcription factors were \ndetected. Results: The number of immature CD1α+DCs in ectopic endometrial lesion tissues \nof EMs group was higher than that of control group whereas the number of mature CD83+DCs \nwas lower than that of control group; uPA, MMP2, MMP9, IL-4, IL-17 and TGF-β contents \nas well as GATA-3, RORγt and FOXP3 expression intensity were higher than those of \ncontrol group, positively correlated with the number of immature CD1α+DCs, and negatively \ncorrelated with the number of mature CD83+DCs; TIMP1, TIMP2 and IFN-γ contents as \nwell as T-bet expression intensity were lower than those of control group, negatively correlated \nwith the number of immature CD1α+DCs, and positively correlated with the number of mature \nCD83+DCs. Conclusion: The disorder of mature and immature DCs in endometrial tissue of \nendometriosis can increase the protease activity and change the Th cellular immune response.