ERGA-BGE Reference Genome of the Western Montpellier Snake (Malpolon monspessulanus), a Key Species for Evolutionary and Venom Studies

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Abstract

Malpolon monspessulanus is a large, rear-fanged snake widely distributed across Mediterranean habitats, where it plays an essential ecological role by controlling populations of small vertebrates. The reference genome of this species offers a crucial resource for uncovering the genomic foundations underlying venom evolution in rear-fanged snakes. A total of 23 contiguous chromosomal pseudomolecules (21 autosomes and two sex chromosomes) were assembled from the genome sequence. This chromosome-level assembly encompasses 1.8 Gb, composed of 140 contigs and 60 scaffolds, with contig and scaffold N50 values of 32.7 Mb and 121.9 Mb, respectively.
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Preprint ARPHA Preprints https://doi.org/10.3897/arphapreprints.e155085 (08 Apr 2025) https://doi.org/10.3897/arphapreprints.e155085 (08 Apr 2025) Submitted to Research Ideas and Outcomes Other versions: - Preprint InfoPreprint Info - CiteCite - MetricsMetrics - CommentComment - RelatedRelated - CitedCited ARPHA Preprints doi: 10.3897/arphapreprints.e155085 First posted 08 Apr 2025 Authors Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Barcelona, Spain CREAC, Centre de Recerca i Educació Ambiental de Calafell (GRENP-Ajuntament de Calafell), C/ Dinamarca 8, 43882, Segur de Calafell, Tarragona, Spain CREAC, Centre de Recerca i Educació Ambiental de Calafell (GRENP-Ajuntament de Calafell), C/ Dinamarca 8, 43882, Segur de Calafell, Tarragona, Spain Institute of Evolutionary Biology (CSIC-Universitat Pompeu Fabra), Barcelona, Spain Institute of Evolutionary Biology (CSIC - Universitat Pompeu Fabra), Barcelona, Spain Leibniz Institute for the Analysis of Biodiversity Change, Museum Koenig Bonn, Adenauerallee 127, 53113, Bonn, Germany Leibniz Institute for the Analysis of Biodiversity Change, Museum Koenig Bonn, Adenauerallee 127, 53113, Bonn, Germany Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac, 4, 08028, Barcelona, Spain Universitat de Barcelona (UB), Barcelona, Spain Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac, 4, 08028, Barcelona, Spain Universitat de Barcelona (UB), 08028, Barcelona, Spain Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac, 4, 08028, Barcelona, Spain Universitat de Barcelona (UB), 08028, Barcelona, Spain Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac, 4, 08028, Barcelona, Spain Universitat de Barcelona (UB), 08028, Barcelona, Spain Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac, 4, 08028, Barcelona, Spain Universitat de Barcelona (UB), 08028, Barcelona, Spain Centro Nacional de Análisis Genómico (CNAG), C/Baldiri Reixac, 4, 08028, Barcelona, Spain Universitat de Barcelona (UB), 08028, Barcelona, Spain European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom Diego De Panis - Corresponding author Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str 17, 10315, Berlin, Germany Berlin Center for Genomics in Biodiversity Research (BeGenDiv), Königin-Luise-Str 6-8, 14195, Berlin, Germany Conflict of interest The authors have declared that no competing interests exist. Supporting agencies Biodiversity Genomics Europe (Grant no.101059492) is funded by Horizon Europe under the Biodiversity, Circular Economy and Environment call (REA.B.3); co-funded by the Swiss State Secretariat for Education, Research and Innovation (SERI) under contract numbers 22.00173 and 24.00054; and by the UK Research and Innovation (UKRI) under the Department for Business, Energy and Industrial Strategy’s Horizon Europe Guarantee Scheme. This research was also made possible by funding from grant PID2021-128901NB-100 MCIN/AEI/10.13039/501100011033 and by ERDF, a way of making Europe. This is an open access preprint distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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