Untapped Potential of Moorochloa eruciformis (Sm.) Veldkamp: Metabolomic Insights into its Antioxidant and in vitro Enzyme-Inhibiting Properties

Untapped Potential of Moorochloa eruciformis (Sm.) Veldkamp: Metabolomic Insights into its Antioxidant and in vitro Enzyme-Inhibiting Properties | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Untapped Potential of Moorochloa eruciformis (Sm.) Veldkamp: Metabolomic Insights into its Antioxidant and in vitro Enzyme-Inhibiting Properties Alia Y. Ragheb, Mona El Shabrawy, Amal G. Hussien, Mona E. S. Kassem, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7790634/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 7 You are reading this latest preprint version Abstract Although unintentionally grown weeds are often seen as a problematic issue because they may compete with desired plants for resources, they can also possess positive and beneficial qualities. In many Poaceae crops, Moorochloa eruciformis (Sm.) Veldkamp is an invasive weed and is often discarded, wasting its potential. To utilize the M. eruciformis , extracts from petroleum ether, 70% aqueous methanol, and defatted aqueous methanol were screened in vitro for their ability to scavenge radicals and inhibit certain diagnostic enzymes. The defatted aqueous methanol exhibited the most significant antioxidant, anti-Alzheimer, and antidiabetic attributes as well as moderate anti-inflammatory effect. Additionally, the same extract was found to contain the major phenolic content as determined spectrophotometrically. Therefore, it was further characterized through LC-ESI-MS in conjunction with GNPS-based molecular networking, aiming to discover the metabolic profile responsible for these impacts. As a result, 102 metabolites were annotated for the first time for the species, encompassing unique derivatives of hydroxycinnamic and hydroxybenzoic acids as well as flavonolignans. M. eruciformis could be recycled and validated as a valuable source of natural phenolic acids and flavonoids, supporting its consideration in clinical research on natural antioxidant and anti-inflammatory agents for arthritis, diabetes, and Alzheimer’s diseases. Biological sciences/Biochemistry Biological sciences/Chemical biology Physical sciences/Chemistry Biological sciences/Drug discovery Biological sciences/Plant sciences Acetylcholinesterase Brachiaria eruciformis Flavonolignans Hydroxybenzoic acid derivatives Molecular networking Weeds Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Review Version 1 posted Reviews received at journal 10 Nov, 2025 Reviewers agreed at journal 10 Nov, 2025 Reviewers invited by journal 10 Nov, 2025 Editor assigned by journal 31 Oct, 2025 Editor invited by journal 24 Oct, 2025 Submission checks completed at journal 22 Oct, 2025 First submitted to journal 22 Oct, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7790634","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":543947756,"identity":"9a183eb8-d284-4f77-bd7e-2e9bbb3c18c6","order_by":0,"name":"Alia Y. 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In many Poaceae crops, \u003cem\u003eMoorochloa eruciformis\u003c/em\u003e (Sm.) Veldkamp is an invasive weed and is often discarded, wasting its potential. To utilize the \u003cem\u003eM. eruciformis\u003c/em\u003e, extracts from petroleum ether, 70% aqueous methanol, and defatted aqueous methanol were screened \u003cem\u003ein vitro\u003c/em\u003e for their ability to scavenge radicals and inhibit certain diagnostic enzymes. The defatted aqueous methanol exhibited the most significant antioxidant, anti-Alzheimer, and antidiabetic attributes as well as moderate anti-inflammatory effect. Additionally, the same extract was found to contain the major phenolic content as determined spectrophotometrically. Therefore, it was further characterized through LC-ESI-MS in conjunction with GNPS-based molecular networking, aiming to discover the metabolic profile responsible for these impacts. 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