Resveratrol induces proliferation and differentiation of mouse preosteoblast MC3T3-E1 by promoting autophagy

Background: The MC3T3-E1 mouse pre-osteoblast can grow and differentiate more rapidly when resveratrol is present, according to study. Uncertainty exists over whether resveratrol (The following grouping is represented as Res) regulates the impact of autophagy on the proliferation and differentiation of mouse pre-osteoblast, despite the fact that autophagy is crucial in the control of osteoblast development. Purpose: To investigate the involvement of autophagy in the proliferation and differentiation of mice pre-osteoblasts as a result of resveratrol. Methods: : 1） MC3T3-E1 cells were separated into blank control group and various concentrations (0.01, 0.1, 1, 10, 100μmol/L) of group in order to determine the ideal resveratrol concentration. In the resveratrol group, CCK8 was used to measure the proliferation activity of pre-osteoblasts in mice in each group after resveratrol intervention; ALP and alizarin red staining were used to gauge the degree of osteogenic differentiation; and RT-qPCR was used to measure the expression levels of Runx2 and OCN in the osteogenic differentiation ability of the cells. 2）In the experiment, four groups were set up: the control group, 3MA, Res group, and Res +3MA group. To examine cell mineralization, ALP and alizarin red staining were utilized. RT-qPCR and Western blot detection of cell autophagy activity levels and osteogenic differentiation capacity in each group following intervention. Results: : 1) Resveratrol might increase the number of mice pre-osteoblast, with the impact being most pronounced at 10μmol/L (P < 0.05); The nodules developed substantially more often than in the blank control group, and Runx2 and OCN expressions significantly increased (P < 0.05); 2) In contrast to the resveratrol group, after 3MA purine blocked autophagy, the Res+3MA group's alkaline phosphatase staining and the development of mineralized nodules were reduced; Runx2, OCN, LC3II / LC3I expression decreased, p62 expression increased (P <0.05). Conclusion: By increasing autophagy, resveratrol may induce osteogenic differentiation of MC3T3-E1 cells.