Carbon substrates promotes stress resistance and drug tolerance in clinical isolates of Candida tropicalis

Carbon substrates promotes stress resistance and drug tolerance in clinical isolates of Candida tropicalis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Carbon substrates promotes stress resistance and drug tolerance in clinical isolates of Candida tropicalis Arpita Khamrai, Saikat Paul, Shivaprakash M Rudramurthy, Anup K. Ghosh This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3867261/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 20 May, 2024 Read the published version in Archives of Microbiology → Version 1 posted 7 You are reading this latest preprint version Abstract Candida tropicalis is a human pathogen and one of the most prevalent non- Candida albicans Candida (NCAC) species causing invasive infections. Azole antifungal resistance in C. tropicalis is also gradually increasing with the increasing incidence of infections. The pathogenic success of C. tropicalis depends on its effective response in the host microenvironment. To become a successful pathogen, cellular metabolism, and physiological status determine the ability of the pathogen to counter diverse stresses inside the host. However, to date, limited knowledge is available on the impact of carbon substrate metabolism on stress adaptation and azole resistance in C. tropicalis . In this study, we determined the impact of glucose, fructose, and sucrose as the sole carbon source on the fluconazole resistance and osmotic (NaCl), oxidative (H 2 O 2 ) stress adaptation in C. tropicalis clinical isolates. We confirmed that the abundance of carbon substrates influences or increases drug resistance and osmotic and oxidative stress tolerance in C. tropicalis . Additionally, both azole-resistant and susceptible isolates showed similar stress adaptation phenotypes, confirming the equal efficiency of becoming successful pathogens irrespective of drug susceptibility profile. To the best of our knowledge, our study is the first on C. tropicalis to demonstrate the direct relation between carbon substrate metabolism and stress tolerance or drug resistance. Candida tropicalis glucose fructose sucrose fluconazole oxidative stress osmotic stress Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryFile1Table.docx SupplementaryFile2Figures.docx Cite Share Download PDF Status: Published Journal Publication published 20 May, 2024 Read the published version in Archives of Microbiology → Version 1 posted Editorial decision: Revision requested 05 Feb, 2024 Reviews received at journal 23 Jan, 2024 Reviewers agreed at journal 22 Jan, 2024 Reviewers invited by journal 21 Jan, 2024 Editor assigned by journal 16 Jan, 2024 Submission checks completed at journal 16 Jan, 2024 First submitted to journal 15 Jan, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3867261","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":267528653,"identity":"503f4ce5-3476-4e25-b6dc-74186c9c8963","order_by":0,"name":"Arpita Khamrai","email":"","orcid":"","institution":"Postgraduate Institute of Medical Education and Research (PGIMER)","correspondingAuthor":false,"prefix":"","firstName":"Arpita","middleName":"","lastName":"Khamrai","suffix":""},{"id":267528654,"identity":"0dcdf141-9ea7-4330-8758-12a8410e44ab","order_by":1,"name":"Saikat Paul","email":"","orcid":"","institution":"Postgraduate Institute of Medical Education and Research (PGIMER)","correspondingAuthor":false,"prefix":"","firstName":"Saikat","middleName":"","lastName":"Paul","suffix":""},{"id":267528655,"identity":"7eab93cb-f66b-40eb-b579-c392f4a4918d","order_by":2,"name":"Shivaprakash M Rudramurthy","email":"","orcid":"","institution":"Postgraduate Institute of Medical Education and Research (PGIMER)","correspondingAuthor":false,"prefix":"","firstName":"Shivaprakash","middleName":"M","lastName":"Rudramurthy","suffix":""},{"id":267528656,"identity":"2523b6c8-a8c6-4aa3-ba60-923425dd9049","order_by":3,"name":"Anup K. 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Azole antifungal resistance in \u003cem\u003eC. tropicalis\u003c/em\u003e is also gradually increasing with the increasing incidence of infections. The pathogenic success of \u003cem\u003eC. tropicalis\u003c/em\u003e depends on its effective response in the host microenvironment. To become a successful pathogen, cellular metabolism, and physiological status determine the ability of the pathogen to counter diverse stresses inside the host. However, to date, limited knowledge is available on the impact of carbon substrate metabolism on stress adaptation and azole resistance in \u003cem\u003eC. tropicalis\u003c/em\u003e. In this study, we determined the impact of glucose, fructose, and sucrose as the sole carbon source on the fluconazole resistance and osmotic (NaCl), oxidative (H\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e2\u003c/sub\u003e) stress adaptation in \u003cem\u003eC. tropicalis\u003c/em\u003e clinical isolates. 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