Generation of maternally semi-cloned mice

Generation of maternally semi-cloned mice | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Generation of maternally semi-cloned mice Jiangwei Lin, Guowei Zou, Yanyan Zhang, Shu Wei, Dingrui Guo, and 6 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-6431826/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Semi-cloning technology has made a series of advances in studying gene function and building disease models. Maternally semi-cloned mice ( MSCM ) have completely identical maternal genome. MSCM are typically produced by injecting sperm into oocytes whose spindles have been replaced by parthenogenetic haploid embryonic stem cells ( PG-haESCs ), resulting in an extremely low birth rate. However, injecting parthenogenetic double haploid ESCs ( PG-DhESCs ) into Prdm14 knockout embryos generates chimeric mice whose germ cells originate from the PG-DhESCs and produce genetically identical oocytes. The offsprings of those chimeric mice, named MSCM, have completely identical maternal genome, mitochondrial genome of the same origin, and an equal proportions of male and female, Biological sciences/Developmental biology/Stem cells/Embryonic stem cells Biological sciences/Developmental biology/Disease model semi cloned mice double haploid embryonic stem cells blastocyst complementation cloned oocytes Full Text Additional Declarations There is NO Competing Interest. Supplementary Files Methods250411.pdf The methods of "Generation of maternally semi-cloned mice " Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-6431826","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":452616360,"identity":"8bebcbcb-a9bc-4672-a778-0038b2332e10","order_by":0,"name":"Jiangwei 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