Integrative Multi-Omics Framework Reveals Microbial and Chemical Biomarkers for Diagnosing Copper Contamination in False Yellowhead–Colonized Soils.

Integrative Multi-Omics Framework Reveals Microbial and Chemical Biomarkers for Diagnosing Copper Contamination in False Yellowhead–Colonized Soils. | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Integrative Multi-Omics Framework Reveals Microbial and Chemical Biomarkers for Diagnosing Copper Contamination in False Yellowhead–Colonized Soils. Quentin Lemoine, Mohamed Bouarfa, Delphine Raviglione, Eve Toulza, and 7 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9208968/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 4 You are reading this latest preprint version Abstract Long-term use of Bordeaux mixture in French vineyards has led to elevated copper levels in soils, many of which are now colonized by false yellowhead after vineyard abandonment. This species provides a relevant model to study plant–soil interactions under copper stress. This study investigated how soil copper contamination affects plant growth in microcosms, focusing on rhizosphere microbial communities and plant metabolomic responses. A multi-omics approach combining metabarcoding and untargeted metabolomics was used to identify biomarkers of copper contamination. Copper levels were quantified in soils, roots, and leaves. The results highlighted altered plant morphology, particularly reduced root growth and copper sequestration in roots. Microbial analyses showed an enrichment of Actinobacteria and Thermoleophilia in highly contaminated soils, while Deltaproteobacteria dominated uncontaminated soils. Metabolomic profiling highlighted flavonoids, quinic acid derivatives, and sesquiterpenes as key chemical markers of copper stress. By integrating microbial and chemical indicators, this study proposes a new framework for diagnosing copper contamination in soil, demonstrating the added value of multi-omic approaches to point out metal-specific stress signatures in soil-plant ecosystems. This study provides new, field-applicable biomarkers for environmental monitoring and open perspectives for assessing biosolution effects on copper-induced abiotic stress in agriculture practices. Copper stress Dittrichia viscosa metabolomics 16S metabarcodings risk Full Text Supplementary Files Supplementarymaterial.docx Cite Share Download PDF Status: Under Review Version 1 posted Reviewers invited by journal 19 Apr, 2026 Editor invited by journal 13 Apr, 2026 Editor assigned by journal 31 Mar, 2026 First submitted to journal 26 Mar, 2026 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. 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