Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide

Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide Xiao-Li Wang, Ru-Nan Zhang, Yu-Lin Pan, Zhi-Ming Li, Hong-Qiu Li, and 10 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3932508/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract The impact of acrylamide (ACR) on learning and memory has garnered considerable attention. However, the targets and mechanisms are still unclear. We used proteomics technology to analyze the serum of the ACR occupationally exposed population and screen out target proteins related to learning and memory. Through in vivo , in vitro experiments, and transgenic mouse models, we investigated the selected target protein to elucidate. Elongation factor 2 (eEF2) was significantly upregulated in the results of serum proteomic. Results from in vitro and in vivo experiments indicated a notable upregulation of Eukaryotic elongation factor 2 kinase (eEF2K), the sole kinase responsible for eEF2 phosphorylation, following exposure to ACR ( P < 0.05). Subsequent in vitro experiments using eEF2K siRNA and in vivo experiments with eEF2K-knockout mice demonstrated significant improvements in abnormal indicators related to ACR-induced learning and memory deficits ( P < 0.05). Proteomic analysis of the hippocampus revealed Lpcat1 as a crucial downstream protein regulated by eEF2K. KEGG analysis indicated that eEF2K may play a role in the process of ACR-induced learning and memory impairment by affecting ether lipid metabolism. In summary, eEF2K as a pivotal target in the mechanisms underlying ACR-induced learning and memory impairment, providing robust evidence for potential clinical interventions targeting. Acrylamide Occupational exposure eEF2K Learning and memory Ether lipid metabolism Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryFigure.docx SupplementaryMethod1.docx SupplementaryMethod2.docx SupplementaryTable.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3932508","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":271274587,"identity":"5795c681-cbfe-4c9f-a2d4-475fe464748d","order_by":0,"name":"Xiao-Li Wang","email":"","orcid":"","institution":"Harbin Medical University","correspondingAuthor":false,"prefix":"","firstName":"Xiao-Li","middleName":"","lastName":"Wang","suffix":""},{"id":271274588,"identity":"010f5f46-6a5a-4a90-97f6-dca434e3499e","order_by":1,"name":"Ru-Nan Zhang","email":"","orcid":"","institution":"Harbin Medical 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metabolism","lastPublishedDoi":"10.21203/rs.3.rs-3932508/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3932508/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eThe impact of acrylamide (ACR) on learning and memory has garnered considerable attention. However, the targets and mechanisms are still unclear. We used proteomics technology to analyze the serum of the ACR occupationally exposed population and screen out target proteins related to learning and memory. Through \u003cem\u003ein vivo\u003c/em\u003e, \u003cem\u003ein vitro\u003c/em\u003e experiments, and transgenic mouse models, we investigated the selected target protein to elucidate. Elongation factor 2 (eEF2) was significantly upregulated in the results of serum proteomic. Results from \u003cem\u003ein vitro\u003c/em\u003e and \u003cem\u003ein vivo\u003c/em\u003e experiments indicated a notable upregulation of Eukaryotic elongation factor 2 kinase (eEF2K), the sole kinase responsible for eEF2 phosphorylation, following exposure to ACR (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05). Subsequent \u003cem\u003ein vitro\u003c/em\u003e experiments using eEF2K siRNA and \u003cem\u003ein vivo\u003c/em\u003e experiments with eEF2K-knockout mice demonstrated significant improvements in abnormal indicators related to ACR-induced learning and memory deficits (\u003cem\u003eP\u003c/em\u003e\u0026thinsp;\u0026lt;\u0026thinsp;0.05). Proteomic analysis of the hippocampus revealed Lpcat1 as a crucial downstream protein regulated by eEF2K. KEGG analysis indicated that eEF2K may play a role in the process of ACR-induced learning and memory impairment by affecting ether lipid metabolism. In summary, eEF2K as a pivotal target in the mechanisms underlying ACR-induced learning and memory impairment, providing robust evidence for potential clinical interventions targeting.\u003c/p\u003e","manuscriptTitle":"Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-02-07 20:45:36","doi":"10.21203/rs.3.rs-3932508/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"ee567e23-ef04-4f29-8b01-c51319160b3c","owner":[],"postedDate":"February 7th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2024-03-19T15:14:59+00:00","versionOfRecord":[],"versionCreatedAt":"2024-02-07 20:45:36","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3932508","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3932508","identity":"rs-3932508","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}