Fufang HuangQi Granule Alleviates Experimental Autoimmune Myasthenia Gravis via IL-17 Signaling Pathway

Fufang HuangQi Granule Alleviates Experimental Autoimmune Myasthenia Gravis via IL-17 Signaling Pathway | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 23 June 2025 V1 Latest version Share on Fufang HuangQi Granule Alleviates Experimental Autoimmune Myasthenia Gravis via IL-17 Signaling Pathway Authors : Shaowen Ye , Junjiu Xu , Han Chen , Yashan Wu , Rencai , Yongqi Liu , Lu Lu , Jing Chen , Wenyi Wu , Ying Tian , Jianhua Sun , and Likun Gong [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.175064984.48479971/v1 147 views 74 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Background: Fufang HuangQi granule (FFHQ), a traditional Chinese medicine formula, has shown clinical efficacy in treating myasthenia gravis; however, the underlying mechanisms remain unclear. This study aimed to explore the therapeutic effects of FFHQ in an experimental autoimmune myasthenia gravis (EAMG) rat model and to elucidate its therapeutic mechanisms. Methods: The EAMG rat model was established, and the rats were treated with FFHQ. The therapeutic effects were evaluated through several indicators, including grip strength, clinical scoring, serum anti-AChR antibody levels, and muscle histopathology. RNA sequencing analysis was subsequently performed on muscle tissues, and a network pharmacology approach was applied to FFHQ. Finally, the mechanisms were further validated by quantitative real-time PCR and immunohistochemistry. Results: FFHQ ameliorated the decrease in muscle grip strength and the increases in clinical scores and serum anti-AChR antibody levels while simultaneously alleviating muscle inflammation symptoms in EAMG rats. As suggested by the RNA-seq and network pharmacology analyses, its efficacy may be associated with the regulation of the IL-17 signaling pathway. RT-qPCR studies revealed that the levels of Tgfb , Il6 , Il1a , Il17a , Nfkb1 , Rela , Cxcl2 , Cxcl6 , and Ccl17 were significantly increased in EAMG rats but decreased after treatment. Immunohistochemical analysis of muscle sections further confirmed a significant reduction in IL-17A expression in the FFHQ administration group compared to the control group. Conclusion: FFHQ improved symptoms in EAMG rats, exerting beneficial effects by modulating the IL-17 signaling pathway. These findings offer a theoretical basis for its utilization. Supplementary Material File (manuscript.docx) Download 72.25 KB Information & Authors Information Version history V1 Version 1 23 June 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Authors Affiliations Shaowen Ye Guangzhou University of Chinese Medicine View all articles by this author Junjiu Xu Nanjing University of Chinese Medicine View all articles by this author Han Chen Chinese Academy of Sciences View all articles by this author Yashan Wu Guangzhou University of Chinese Medicine View all articles by this author Rencai Zhejiang Jiuru Pharmaceutical Technology Co Ltd View all articles by this author Yongqi Liu Chinese Academy of Sciences View all articles by this author Lu Lu Chinese Academy of Sciences View all articles by this author Jing Chen Shanghai Institute of Materia Medica Chinese Academy of Sciences View all articles by this author Wenyi Wu Chinese Academy of Sciences View all articles by this author Ying Tian Chinese Academy of Sciences View all articles by this author Jianhua Sun Chinese Academy of Sciences View all articles by this author Likun Gong [email protected] Guangzhou University of Chinese Medicine View all articles by this author Metrics & Citations Metrics Article Usage 147 views 74 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Shaowen Ye, Junjiu Xu, Han Chen, et al. Fufang HuangQi Granule Alleviates Experimental Autoimmune Myasthenia Gravis via IL-17 Signaling Pathway. Authorea . 23 June 2025. DOI: https://doi.org/10.22541/au.175064984.48479971/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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