Trichoderma and agrochemical induced defensive metabolites for suppressing rust disease of faba bean in Bangladesh

Trichoderma and agrochemical induced defensive metabolites for suppressing rust disease of faba bean in Bangladesh | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Trichoderma and agrochemical induced defensive metabolites for suppressing rust disease of faba bean in Bangladesh Faria Noshin, Zaid Bin Hossain, Tawseef Al AFF, Sadia Afrin Shila, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-9187735/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Research investigations were conducted both in in-vitro and in-vivo with three potential strains of Trichoderma isolates ( Trichoderma harzianum AB935952, T. asperellum AB935955, and T. viride, Tv6 ); three agrochemicals viz. , Bion, Chitosan and Amistar Top (as positive control) during two cropping season (2019–2021) to manage rust disease of faba bean in Mymensingh district of Bangladesh. In vitro assay clearly revealed that 60% culture filtrate of T. viride; bion and Amistar top @ 60 mM inhibited 100% inhibition of uredial germination of Uromyces viciae fabae followed by T. harzianum and T. asperellum . Chitosan showed significantly the lowest inhibition of urediospore germination. Spraying of each Tricho-suspension (@10 8 cfu/ml) and agrochemicals on faba bean plants in the greenhouse significantly decreased rusty spot severity where, Amistar top, T. viride and Bion were found to be the most effective treatments followed by T. harzianum and T. asperellum . Five treatments were found significantly effective in reducing the rust disease severity of faba bean comparing with control treatment under field conditions. In both cropping seasons, field experiments revealed that spraying of Trichoderma spp., Bion and Amistar Top on faba bean plants significantly reduced the rust disease severity and increased the agronomic parameters viz ., plant height (cm), no. of pods /plant, fresh wt (g), dry weight (g) and seed yield (kg/ha) compared with the control treatment. Among the three Trichoderma spp., T. viride showed the best performance in reducing rust disease severity (8.20% and 10.20%) with increasing faba bean yield (3240 and 3331 kg/ha) followed by Bion. Analysis of induced biochemicals, Bion resulted the highest activity of peroxidase (POX), polyphenoloxidase (PPO), and phenylalanine ammonia lyase (PAL), whereas, T. viride resulted the highest amount of total phenol and flavonoid. T. harzianum resulted the highest amount of β, 1–3 glucanase and total chlorophyll content. The least amount of bio-chemicals with higher rust severity was recorded in case of Chitosan. Thus, the increments of POX activity, total phenols, total flavonoids and seed yield revealed that T. viride is a promising antagonistic species of fungi that may be the alternate of chemical fungicides for management of rust disease in Bangladesh. Trichoderma Defensive metabolites Faba bean Rust disease Control Yield Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-9187735","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":615796417,"identity":"6fd05864-ecf0-4e05-b283-1698e5eab2d5","order_by":0,"name":"Faria Noshin","email":"","orcid":"","institution":"Bangladesh Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Faria","middleName":"","lastName":"Noshin","suffix":""},{"id":615796418,"identity":"fee1e7a2-c234-40bf-8741-bd16f065fb7d","order_by":1,"name":"Zaid Bin Hossain","email":"","orcid":"","institution":"Rajshahi University of Engineering and Technology","correspondingAuthor":false,"prefix":"","firstName":"Zaid","middleName":"Bin","lastName":"Hossain","suffix":""},{"id":615796419,"identity":"bb342201-0b6c-4e04-88f0-925156126fc5","order_by":2,"name":"Tawseef Al AFF","email":"","orcid":"","institution":"Bangladesh Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Tawseef","middleName":"Al","lastName":"AFF","suffix":""},{"id":615796420,"identity":"bb358aae-dba0-4a8e-8e83-f07d23fda2de","order_by":3,"name":"Sadia Afrin Shila","email":"","orcid":"","institution":"Bangladesh Agricultural University","correspondingAuthor":false,"prefix":"","firstName":"Sadia","middleName":"Afrin","lastName":"Shila","suffix":""},{"id":615796421,"identity":"480af4bc-d76f-4698-8f1e-9a5e5ccc5a14","order_by":4,"name":"Mona H. 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Chitosan showed significantly the lowest inhibition of urediospore germination. Spraying of each Tricho-suspension (@10\u003csup\u003e8\u003c/sup\u003ecfu/ml) and agrochemicals on faba bean plants in the greenhouse significantly decreased rusty spot severity where, Amistar top, \u003cem\u003eT. viride\u003c/em\u003e and Bion were found to be the most effective treatments followed by \u003cem\u003eT. harzianum\u003c/em\u003e and \u003cem\u003eT. asperellum\u003c/em\u003e. Five treatments were found significantly effective in reducing the rust disease severity of faba bean comparing with control treatment under field conditions. In both cropping seasons, field experiments revealed that spraying of \u003cem\u003eTrichoderma\u003c/em\u003e spp., Bion and Amistar Top on faba bean plants significantly reduced the rust disease severity and increased the agronomic parameters \u003cem\u003eviz\u003c/em\u003e., plant height (cm), no. of pods /plant, fresh wt (g), dry weight (g) and seed yield (kg/ha) compared with the control treatment. Among the three \u003cem\u003eTrichoderma\u003c/em\u003e spp., \u003cem\u003eT. viride\u003c/em\u003e showed the best performance in reducing rust disease severity (8.20% and 10.20%) with increasing faba bean yield (3240 and 3331 kg/ha) followed by Bion. Analysis of induced biochemicals, Bion resulted the highest activity of peroxidase (POX), polyphenoloxidase (PPO), and phenylalanine ammonia lyase (PAL), whereas, \u003cem\u003eT. viride\u003c/em\u003e resulted the highest amount of total phenol and flavonoid. \u003cem\u003eT. harzianum\u003c/em\u003e resulted the highest amount of β, 1\u0026ndash;3 glucanase and total chlorophyll content. The least amount of bio-chemicals with higher rust severity was recorded in case of Chitosan. Thus, the increments of POX activity, total phenols, total flavonoids and seed yield revealed that \u003cem\u003eT. viride\u003c/em\u003e is a promising antagonistic species of fungi that may be the alternate of chemical fungicides for management of rust disease in Bangladesh.\u003c/p\u003e","manuscriptTitle":"Trichoderma and agrochemical induced defensive metabolites for suppressing rust disease of faba bean in Bangladesh","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-04-06 15:54:17","doi":"10.21203/rs.3.rs-9187735/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"04d43860-4c64-43c6-94d6-d9ff3e94a559","owner":[],"postedDate":"April 6th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-04-28T14:08:24+00:00","versionOfRecord":[],"versionCreatedAt":"2026-04-06 15:54:17","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-9187735","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-9187735","identity":"rs-9187735","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}