TheDrosophilaCircadian Clock GeneCycleControls the Development of Clock Neurons

Daily behavioral and physiological rhythms are controlled by the brain’s circadian timekeeping system, a synchronized network of neurons that maintains endogenous molecular oscillations. These oscillations are based on transcriptional feedback loops of clock genes, which in Drosophila include the transcriptional activators Clock (Clk) and cycle (cyc) . While the mechanisms underlying this molecular clock are very well characterized, the roles that the core clock genes play in neuronal physiology and development are much less understood. The Drosophila timekeeping center is composed of ∼150 clock neurons, among which the four small ventral lateral neurons (sLN v s) are the most dominant pacemakers under constant conditions. Here, we show that downregulating the clock gene cyc specifically in the Pdf -expressing neurons prevents leads to decreased fasciculation both in larval and adult brains. This effect is due to a developmental role of cyc , as both knocking down cyc or expressing a dominant negative form of cyc exclusively during development lead to defasciculation phenotypes in adult clock neurons. Clk downregulation also leads to developmental effects on sLNv morphology, although cyc and Clk manipulations produce distinct phenotypes. Our results reveal a non-circadian role for cyc , shedding light on the additional functions of circadian clock genes in the development of the nervous system.