Pathogen-specific IgE-reactive cytosolic allergenic epitopes of Aspergillus fumigatus for immunodiagnostic/immunotherapeutic applications against allergic aspergillosis

Pathogen-specific IgE-reactive cytosolic allergenic epitopes of Aspergillus fumigatus for immunodiagnostic/immunotherapeutic applications against allergic aspergillosis | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Pathogen-specific IgE-reactive cytosolic allergenic epitopes of Aspergillus fumigatus for immunodiagnostic/immunotherapeutic applications against allergic aspergillosis Bharat Singh, Priya Koundal, Sunita Manhas, Mehak Dangi, Gainda L. Sharma, and 7 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4526742/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Aspergillus fumigatus is the causative agent of a global infectious respiratory disease (aspergillosis). Its accurate diagnosis is difficult due to overlapping clinical/radiological features with other respiratory infections. Thus, to identify A. fumigatus-specific entities for more comprehensive use in the immunodiagnosis/immunotherapy of aspergillosis, this work was conducted. We recruited aspergillosis patients after clinical diagnosis; the results included immunoreactivity to A. fumigatus crude/recombinant allergens. Furthermore, 2DE immunoblotting of the A. fumigatus cytosolic fraction was performed with the sera of 10 individual patients, and subsequent Q-TOF analysis of the corresponding IgE-reactive spots from the 2DE-gles identified 18 proteins. These 18 allergenic proteins included 2 known (Asp-f12 and Asp-f22) and 3 predicted allergens (sorbitol/xylulose-reductase, Hsp70-chaperone Hsp88, and Hsp70) of A. fumigatus that exhibited cross-reactivity with other fungal allergens. However, 11 other 13 allergenic proteins from A. fumigatus contained significant immunoreactivity to aspergillosis patient sera. Furthermore, in silico analysis of the antigenic epitopes and cross-reactivity predictions revealed A. fumigatus-specific 9/B-cell and 4/T-cell epitopes. Subsequent docking of representative B-cell and T-cell epitopes with the respective B-cell receptor and MHC-II complex also confirmed receptor-specific binding. These identified pathogen-specific allergens and epitopes could be valuable for future immunodiagnostic and immunotherapeutic applications for better management of aspergillosis and for reducing the disease burden globally. Biological sciences/Immunology Health sciences/Biomarkers Health sciences/Diseases Allergens Antibody Aspergillosis Diagnosis Immunoproteomics Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryMaterial.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4526742","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":314604308,"identity":"cc5e8fba-923d-4da6-9fd3-8191b89229ec","order_by":0,"name":"Bharat Singh","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABFElEQVRIiWNgGAWjYBACAwkIzQNCBz4YSMiBeAceENSSANbCeHBGhYUxWEsCEVpAFjEf5jhTkdgAF8ABzKWbn334+aNOxpx/7YHDjG0S6fPDDj8E2mInp9uAXYvlnGPGM3sSDvNYzniXcLiwTSJ34+00A6CWZGOzAzgcdiPBmIEn4QCPwY0zBodngrTMTgBpOZC4DaeW9M+MfxLqIFp4gQ4znJ3+gYCWHGNmngRmHoPzPQaHec5IJMhL5+C3xXJGTjGzTNphoC08BsBAljDcIJ1TcCDBALdfzCXSNzO+samzNzh/xvjDB4M6efnZ6Zs/fKiwk8OlBQEkEqBOBas0IKQcBPihhso3EKN6FIyCUTAKRhIAAK+9Z61E9fEFAAAAAElFTkSuQmCC","orcid":"","institution":"MMEC, Maharishi Markandeshwar (Deemed to be University)","correspondingAuthor":true,"prefix":"","firstName":"Bharat","middleName":"","lastName":"Singh","suffix":""},{"id":314604309,"identity":"216bedfe-80cd-4ca7-bae7-45817ef2ef91","order_by":1,"name":"Priya Koundal","email":"","orcid":"","institution":"MM Institute of Medical Sciences and Research, Maharishi Markandeshwar (Deemed to be University)","correspondingAuthor":false,"prefix":"","firstName":"Priya","middleName":"","lastName":"Koundal","suffix":""},{"id":314604310,"identity":"65a14516-cff4-45b6-bee9-abb0f9af56db","order_by":2,"name":"Sunita Manhas","email":"","orcid":"","institution":"MM Institute of Medical Sciences and Research, Maharishi Markandeshwar (Deemed to be University)","correspondingAuthor":false,"prefix":"","firstName":"Sunita","middleName":"","lastName":"Manhas","suffix":""},{"id":314604311,"identity":"cfc22ba2-bd30-40bb-acb4-f3d8d55ca09c","order_by":3,"name":"Mehak Dangi","email":"","orcid":"","institution":"Maharshi Dayanand University","correspondingAuthor":false,"prefix":"","firstName":"Mehak","middleName":"","lastName":"Dangi","suffix":""},{"id":314604312,"identity":"451c66c4-c058-49d6-a0cc-257450c5c2f8","order_by":4,"name":"Gainda L. 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Its accurate diagnosis is difficult due to overlapping clinical/radiological features with other respiratory infections. Thus, to identify A. fumigatus-specific entities for more comprehensive use in the immunodiagnosis/immunotherapy of aspergillosis, this work was conducted. We recruited aspergillosis patients after clinical diagnosis; the results included immunoreactivity to A. fumigatus crude/recombinant allergens. Furthermore, 2DE immunoblotting of the A. fumigatus cytosolic fraction was performed with the sera of 10 individual patients, and subsequent Q-TOF analysis of the corresponding IgE-reactive spots from the 2DE-gles identified 18 proteins. These 18 allergenic proteins included 2 known (Asp-f12 and Asp-f22) and 3 predicted allergens (sorbitol/xylulose-reductase, Hsp70-chaperone Hsp88, and Hsp70) of A. fumigatus that exhibited cross-reactivity with other fungal allergens. However, 11 other 13 allergenic proteins from A. fumigatus contained significant immunoreactivity to aspergillosis patient sera. Furthermore, in silico analysis of the antigenic epitopes and cross-reactivity predictions revealed A. fumigatus-specific 9/B-cell and 4/T-cell epitopes. Subsequent docking of representative B-cell and T-cell epitopes with the respective B-cell receptor and MHC-II complex also confirmed receptor-specific binding. 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