Chemical composition and antibacterial properties of curcuma longa volatile oil and solvents extracts against selected clinical bacteria

Chemical composition and antibacterial properties of curcuma longa volatile oil and solvents extracts against selected clinical bacteria | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Chemical composition and antibacterial properties of curcuma longa volatile oil and solvents extracts against selected clinical bacteria Kafayat kemi saliu, Adebisi Olonisakin, Olusegun RIchard Adeoyo, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8941794/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Turmeric ( Curcuma Longa ) rhizome is a natural product of plant that is usually consumed. Its bioactive compounds can be explored to control bacterial growth. This study evaluated the chemical composition of turmeric volatile oil and extracts against clinical bacteria. Bioactive compounds of turmeric rhizome were subjected to hydrodistillation using Clevenger apparatus. The non volatile extracts were carried out using Hexane and Methanol. The oil and the extracts were subjected to gas chromatography-mass spectrometry (GC-MS) and were evaluated for their antibacterial activity by agar well diffusion assay. Minimum inhibitory concentration (MIC) was determined using the standard method. The results showed that eighty-four (84) compounds were identified in turmeric volatile oil with ar-turmerone (19.01%), cedr-8-ene (10.13%), and β-terpinene (10.01%) being predominant. The main constituents of turmeric n-hexane were turmerone (13.39%) and14-beta-H-pregna (9.05%). Methanol extracts contained turmerone (12.46%) and 14-beta-H-pregna (8.72%) respectively. Findings revealed that volatile oil and the extracts have antibacterial properties against S. aureus, K. ornithinolytica , and C. gillenii , turmeric n-hexane extract had the highest zone of inhibition (28 ± 1.03 mm) against C. gillenii followed by K. ornithinolytica (26 ± 1.63 mm), and S. aureus (25 ± 0.33 mm). The MIC results showed that turmeric volatile oil showed considerable activity against K. ornithinolytica at concentration of 6.25 mg/ml than for all other bacteria. In conclusion, the volatile oils of turmeric oil and extracts have potentials for use as a natural agent against some clinical bacterial species. Organic Chemistry Turmeric Chemical composition Antibacterial agents Clinical bacterial isolates Full Text Additional Declarations The authors declare no competing interests. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8941794","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":595268936,"identity":"9493282c-f3e6-450b-a489-e56db21d1504","order_by":0,"name":"Kafayat kemi 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interests.","formattedTitle":"\u003cp\u003eChemical composition and antibacterial properties of curcuma longa volatile oil and solvents extracts against selected clinical bacteria\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Adekunle Ajasin University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Turmeric, Chemical composition, Antibacterial agents, Clinical bacterial isolates","lastPublishedDoi":"10.21203/rs.3.rs-8941794/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-8941794/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eTurmeric (\u003cem\u003eCurcuma Longa\u003c/em\u003e) rhizome is a natural product of plant that is usually consumed. Its bioactive compounds can be explored to control bacterial growth. This study evaluated the chemical composition of turmeric volatile oil and extracts against clinical bacteria.\u003c/p\u003e \u003cp\u003eBioactive compounds of turmeric rhizome were subjected to hydrodistillation using Clevenger apparatus. The non volatile extracts were carried out using Hexane and Methanol. The oil and the extracts were subjected to gas chromatography-mass spectrometry (GC-MS) and were evaluated for their antibacterial activity by agar well diffusion assay. Minimum inhibitory concentration (MIC) was determined using the standard method.\u003c/p\u003e \u003cp\u003eThe results showed that eighty-four (84) compounds were identified in turmeric volatile oil with ar-turmerone (19.01%), cedr-8-ene (10.13%), and β-terpinene (10.01%) being predominant. The main constituents of turmeric n-hexane were turmerone (13.39%) and14-beta-H-pregna (9.05%). Methanol extracts contained turmerone (12.46%) and 14-beta-H-pregna (8.72%) respectively. Findings revealed that volatile oil and the extracts have antibacterial properties against \u003cem\u003eS. aureus, K. ornithinolytica\u003c/em\u003e, and \u003cem\u003eC. gillenii\u003c/em\u003e, turmeric n-hexane extract had the highest zone of inhibition (28\u0026thinsp;\u0026plusmn;\u0026thinsp;1.03 mm) against \u003cem\u003eC. gillenii\u003c/em\u003e followed by \u003cem\u003eK. ornithinolytica\u003c/em\u003e (26\u0026thinsp;\u0026plusmn;\u0026thinsp;1.63 mm), and \u003cem\u003eS. aureus\u003c/em\u003e (25\u0026thinsp;\u0026plusmn;\u0026thinsp;0.33 mm). The MIC results showed that turmeric volatile oil showed considerable activity against \u003cem\u003eK. ornithinolytica\u003c/em\u003e at concentration of 6.25 mg/ml than for all other bacteria.\u003c/p\u003e \u003cp\u003eIn conclusion, the volatile oils of turmeric oil and extracts have potentials for use as a natural agent against some clinical bacterial species.\u003c/p\u003e","manuscriptTitle":"Chemical composition and antibacterial properties of curcuma longa volatile oil and solvents extracts against selected clinical bacteria","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2026-02-24 16:04:59","doi":"10.21203/rs.3.rs-8941794/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"0d46907d-5e2b-4bfc-85a5-cb9a72ec26cd","owner":[],"postedDate":"February 24th, 2026","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":63341209,"name":"Organic Chemistry"}],"tags":[],"updatedAt":"2026-02-24T16:04:59+00:00","versionOfRecord":[],"versionCreatedAt":"2026-02-24 16:04:59","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8941794","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8941794","identity":"rs-8941794","version":["v1"]},"buildId":"XKTyCvWXoU3ODBz1xrDgd","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}