Quantitative evaluation of zinc phthalocyanine photosensitized production by singlet oxygen probe

Quantitative evaluation of zinc phthalocyanine photosensitized production by singlet oxygen probe | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Quantitative evaluation of zinc phthalocyanine photosensitized production by singlet oxygen probe Hailong Feng, Yongliang Li, Jiahang Li, Ning Wang, Jiawei Shangguan This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5211692/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract With the widespread application and development of photodynamic therapy (PDT) in cancer treatment, how to accurately quantify photodynamic dose for real-time adjustment and optimization of dose has become a hot topic in PDT research. This study utilized singlet oxygen probes to investigate the photophysical process of zinc phthalocyanine (ZnPc) generating singlet oxygen ( 1 O 2 ) under light excitation conditions. We analyzed the absorbance changes of the mixed solution of ZnPc and 1,3-diphenylisobenzofuran (DPBF) over time under varying light fluence rates. The experimental results indicated that after 90 s irradiations at light fluence rates of 40 mW/cm², 30 mW/cm², 20 mW/cm², and 10 mW/cm², the consumptions of DPBF were 77.5%, 62.7%, 50.2%, and 32.4%, respectively. The total productions of 1 O 2 were calculated to be 1375.8 μM, 882.5 μM, 631.7 μM, and 364.1 μM, respectively. This successfully linked the concentration changes of the singlet oxygen probe with the total production of singlet oxygen, enhancing the accuracy of photodynamic dose research. Photodynamic therapy singlet oxygen production light dose study DPBF Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5211692","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":364970811,"identity":"fbcc1536-8c20-4c15-83d3-f868195a25f7","order_by":0,"name":"Hailong Feng","email":"","orcid":"","institution":"Changchun University of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Hailong","middleName":"","lastName":"Feng","suffix":""},{"id":364970812,"identity":"89b6ce82-e172-4422-b40d-86e6b5851516","order_by":1,"name":"Yongliang Li","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA/UlEQVRIiWNgGAWjYBACPmYwZcEDJBgfQAUN8Gphg2iRAGlhBimVIKwFQkmA2RLEaWHnMXxc8EtChn92+7WKn211dQzszdskGGru4HEYj7HxzD4JHok7Z8pu9rYdlmDgOVYmwXDsGT4tZtK8PUC/3MhJu83YdkCCQSLHTIKx4TBhLfJALcWMbXUSDPJviNDC80OCx+BG+jFmxjZmoC08hLSwFRvzNkjwGN7IYZbsOXdYso0nrdgi4RhuLfz8hzc+5vljYy93I/3hhx9ldfz87Ic33vhQg1sLAwOHAQNjG4jBA4kOcEwl4NHAwMD+gIHhD4wxCkbBKBgFowALAAAjYUXB85cpWgAAAABJRU5ErkJggg==","orcid":"","institution":"Changchun University of Science and Technology","correspondingAuthor":true,"prefix":"","firstName":"Yongliang","middleName":"","lastName":"Li","suffix":""},{"id":364970813,"identity":"7d199bd0-baf1-46b2-8945-2b9ef7ad7456","order_by":2,"name":"Jiahang Li","email":"","orcid":"","institution":"Changchun University of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Jiahang","middleName":"","lastName":"Li","suffix":""},{"id":364970814,"identity":"fc7cff31-1532-40a1-a964-c5ba688817a9","order_by":3,"name":"Ning Wang","email":"","orcid":"","institution":"Changchun University of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Ning","middleName":"","lastName":"Wang","suffix":""},{"id":364970815,"identity":"0aefbe9f-d711-4a2c-a5b8-84734adeda36","order_by":4,"name":"Jiawei Shangguan","email":"","orcid":"","institution":"Changchun University of Science and Technology","correspondingAuthor":false,"prefix":"","firstName":"Jiawei","middleName":"","lastName":"Shangguan","suffix":""}],"badges":[],"createdAt":"2024-10-06 07:23:10","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-5211692/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-5211692/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":70710524,"identity":"a8da0c67-b083-477f-8a76-c15c8e5a546a","added_by":"auto","created_at":"2024-12-05 23:46:29","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":666739,"visible":true,"origin":"","legend":"","description":"","filename":"manuscriptfordoubleanonymouspeer.pdf","url":"https://assets-eu.researchsquare.com/files/rs-5211692/v1_covered_a504aae0-2997-4c86-9983-4193c5d74630.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Quantitative evaluation of zinc phthalocyanine photosensitized production by singlet oxygen probe","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Photodynamic therapy, singlet oxygen production, light dose study, DPBF","lastPublishedDoi":"10.21203/rs.3.rs-5211692/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-5211692/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eWith the widespread application and development of photodynamic therapy (PDT) in cancer treatment, how to accurately quantify photodynamic dose for real-time adjustment and optimization of dose has become a hot topic in PDT research. This study utilized singlet oxygen probes to investigate the photophysical process of zinc phthalocyanine (ZnPc) generating singlet oxygen (\u003csup\u003e1\u003c/sup\u003eO\u003csub\u003e2\u003c/sub\u003e) under light excitation conditions. We analyzed the absorbance changes of the mixed solution of ZnPc and 1,3-diphenylisobenzofuran (DPBF) over time under varying light fluence rates. The experimental results indicated that after 90 s irradiations at light fluence rates of 40 mW/cm², 30 mW/cm², 20 mW/cm², and 10 mW/cm², the consumptions of DPBF were 77.5%, 62.7%, 50.2%, and 32.4%, respectively. The total productions of \u003csup\u003e1\u003c/sup\u003eO\u003csub\u003e2\u003c/sub\u003e were calculated to be 1375.8 μM, 882.5 μM, 631.7 μM, and 364.1 μM, respectively. 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