Fusarium species variations in commercial onion fields in Northeastern Israel

Fusarium species variations in commercial onion fields in Northeastern Israel | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Fusarium species variations in commercial onion fields in Northeastern Israel Ofir Degani, Elhanan Dimant, Eliyahu Margalit This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3058884/v2 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 06 Apr, 2024 Read the published version in Horticulturae → Version 2 posted You are reading this latest preprint version Show more versions Abstract Fusarium basal rot (FBR) is a significant limitation to Allium production worldwide. The damage of the disease can be observed throughout the entire crop cycle. The current research aims to understand better the impact of Fusarium Basal Rot (FBR) on the cultivation of onions ( Allium cepa ) in northeast Israel. It focuses on studying the composition and incidence of Fusarium species involved in the disease outburst in two representative fields, one in the Galilee (Hula Valley) and the second in the Golan Heights, where the disease incidences reached 8%. Using colony morphology, microscopic taxonomic keys, and molecular methods, a new, unreported Fusarium solani species was discovered as a wildly spread member of the Fusarium pathobiome community. This species appeared to be more generalist in its nature since it was found in all three onion cultivars' samples. It was also less virulent in seed germination (42-52% higher sprout biomass, p < 0.05) and bulb pathogenicity tests (41-45% less necrotic) than Fusarium acutatum . Whereas the Galilee yellow Orlando onion cultivar bulbs sampled are colonized by F. solani (70%) and two other, less abundant species, F. oxysporum , f. sp. cepae , and F. acutatum (15% each), the Golan Heights field's Fusarium community, show host specificity. In the Golan Heights field, F. oxysporum f. sp. cepae inhabited the red H1 onion cultivar bulbs, whereas F. acutatum colonized the yellow H2 cultivar (40% and 50% prevalence along with F. solani ). A better understanding of this disease complexity caused by different Fusarium species with a divergence in host susceptibility and virulence is critical for developing disease management strategies. Since each Fusarium species reacts differently to pest control treatments, the changes in the species composition may require specifically adapted pest control solutions. Agronomy Allium cepa basal rot distribution fungus Fusarium onion pathogenicity assay PCR Full Text Additional Declarations The authors declare no competing interests. Supplementary Files FigureS1.docx FigureS2.docx FigureS3.docx TableS1.docx Cite Share Download PDF Status: Published Journal Publication published 06 Apr, 2024 Read the published version in Horticulturae → Version 2 posted You are reading this latest preprint version Show more versions Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3058884","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":215149194,"identity":"a8bc2ffe-ab3d-482c-85b2-633d5b8f9f13","order_by":0,"name":"Ofir 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Israel\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Allium cepa, basal rot, distribution, fungus, Fusarium, onion, pathogenicity assay, PCR ","lastPublishedDoi":"10.21203/rs.3.rs-3058884/v2","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3058884/v2","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eFusarium basal rot (FBR) is a significant limitation to \u003cem\u003eAllium\u003c/em\u003eproduction worldwide. The damage of the disease can be observed throughout the entire crop cycle. The current research aims to understand better the impact of Fusarium Basal Rot (FBR) on the cultivation of onions (\u003cem\u003eAllium cepa\u003c/em\u003e) in northeast Israel. It focuses on studying the composition\u003cem\u003e \u003c/em\u003eand incidence of \u003cem\u003eFusarium \u003c/em\u003especies\u003cem\u003e \u003c/em\u003einvolved in the disease outburst in two representative fields, one in the Galilee (Hula Valley) and the second in the Golan Heights, where the disease incidences reached 8%. Using colony morphology, microscopic taxonomic keys, and molecular methods, a new, unreported\u003cem\u003e Fusarium solani\u003c/em\u003especies was discovered as a wildly spread member of the \u003cem\u003eFusarium\u003c/em\u003e pathobiome community. This species appeared to be more generalist in its nature since it was found in all three onion cultivars' samples. It was also less virulent in seed germination (42-52% higher sprout biomass, \u003cem\u003ep\u003c/em\u003e \u0026lt; 0.05) and bulb pathogenicity tests (41-45% less necrotic) than \u003cem\u003eFusarium\u003c/em\u003e \u003cem\u003eacutatum\u003c/em\u003e. Whereas the Galilee yellow Orlando onion cultivar bulbs sampled are colonized by \u003cem\u003eF. solani\u003c/em\u003e (70%) and two other, less abundant species, \u003cem\u003eF. oxysporum\u003c/em\u003e, f. sp. \u003cem\u003ecepae\u003c/em\u003e, and \u003cem\u003eF. acutatum \u003c/em\u003e(15% each), the Golan Heights field's \u003cem\u003eFusarium\u003c/em\u003e community, show host specificity. In the Golan Heights field, \u003cem\u003eF. oxysporum\u003c/em\u003e f. sp. \u003cem\u003ecepae\u003c/em\u003e inhabited the red H1 onion cultivar bulbs, whereas \u003cem\u003eF. acutatum\u003c/em\u003e colonized the yellow H2 cultivar (40% and 50% prevalence along with \u003cem\u003eF. solani\u003c/em\u003e). A better understanding of this disease complexity caused by different \u003cem\u003eFusarium\u003c/em\u003e species with a divergence in host susceptibility and virulence is critical for developing disease management strategies. Since each \u003cem\u003eFusarium\u003c/em\u003e species reacts differently to pest control treatments, the changes in the species composition may require specifically adapted pest control solutions.\u003c/p\u003e","manuscriptTitle":"Fusarium species variations in commercial onion fields in Northeastern Israel","msid":"","msnumber":"","nonDraftVersions":[{"code":2,"date":"2024-01-04 19:30:15","doi":"10.21203/rs.3.rs-3058884/v2","editorialEvents":[{"type":"communityComments","content":1}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}},{"code":1,"date":"2023-07-03 17:53:29","doi":"10.21203/rs.3.rs-3058884/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"25e05af0-cc1b-4766-a2b5-bfb6550470d9","owner":[],"postedDate":"January 4th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":27882774,"name":"Agronomy"}],"tags":[],"updatedAt":"2024-04-09T00:54:31+00:00","versionOfRecord":{"articleIdentity":"rs-3058884","link":"https://doi.org/10.3390/horticulturae10040373","journal":{"identity":"horticulturae","isVorOnly":true,"title":"Horticulturae"},"publishedOn":"2024-04-07 00:00:00","publishedOnDateReadable":"April 7th, 2024"},"versionCreatedAt":"2024-01-04 19:30:15","video":"","vorDoi":"10.3390/horticulturae10040373","vorDoiUrl":"https://doi.org/10.3390/horticulturae10040373","workflowStages":[]},"version":"v2","identity":"rs-3058884","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3058884","identity":"rs-3058884","version":["v2"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}