Unveiling the Antidiabetic Potential of Achyranthes aspera In Vitro and In Silico Efficacy Insights

Unveiling the Antidiabetic Potential of Achyranthes aspera In Vitro and In Silico Efficacy Insights | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Unveiling the Antidiabetic Potential of Achyranthes aspera In Vitro and In Silico Efficacy Insights Jing Zhao, Zhang Yu Ming, Ibrahim Mahmood, Shazrul Fazry, Muhammad Ismail, and 4 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-5777722/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Diabetes mellitus (DM) is a prevalent metabolic disorder with increasing global incidence. Current pharmaceutical treatments, such as insulin and oral hypoglycemic agents, are often associated with side effects and drug resistance, this underscores the need for safer alternatives. Achyranthes aspera , a plant with a long history of use in traditional medicine, has demonstrated potential in managing diabetes. This study employs both in vitro and in silico approaches to assess the antidiabetic efficacy of A. aspera . In vitro assays were conducted to evaluate the effects of the plant on key enzymes involved in glycemic control and carbohydrate metabolism, namely DPP-4, α-amylase, and α-glucosidase. Significant inhibition of these enzymes was observed, indicating the potential of A. aspera to regulate blood glucose levels effectively. Additionally, cytotoxicity assays using L6 cell lines demonstrated that A. aspera extract enhances glucose uptake and remains nontoxic at therapeutic concentrations, suggesting an improvement in insulin sensitivity. Molecular docking studies further supported these findings, which revealed strong interactions between bioactive compounds in A. aspera and target proteins involved in glucose metabolism. In summary, these results underscore the therapeutic potential of A. aspera as a natural antidiabetic agent. However, further in vivo studies are recommended to validate its clinical efficacy and explore its potential for use in diabetes treatment. Biological sciences/Computational biology and bioinformatics/Computational models Physical sciences/Chemistry/Photochemistry Biological sciences/Chemical biology/Natural products Biological sciences/Biological techniques Biological sciences/Cell biology Achyranthes aspera Antidiabetic activity In vitro analysis Diabetes mellitus management human health Full Text Additional Declarations No competing interests reported. Supplementary Files SupplementaryMateria1.xlsx SupplementaryMaterial2.xlsx SupplementaryMaterial3A.docx SupplementaryMaterial3B.docx SupplementaryMaterial4A.docx SupplementaryMaterial4B.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-5777722","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":449324264,"identity":"7abdaeda-1978-41be-9763-cc2db9d46a23","order_by":0,"name":"Jing Zhao","email":"","orcid":"","institution":"Tarim University","correspondingAuthor":false,"prefix":"","firstName":"Jing","middleName":"","lastName":"Zhao","suffix":""},{"id":449324266,"identity":"4bff8965-edfc-4eff-b392-27888176411e","order_by":1,"name":"Zhang Yu Ming","email":"","orcid":"","institution":"INTI International 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Current pharmaceutical treatments, such as insulin and oral hypoglycemic agents, are often associated with side effects and drug resistance, this underscores the need for safer alternatives. \u003cem\u003eAchyranthes aspera\u003c/em\u003e, a plant with a long history of use in traditional medicine, has demonstrated potential in managing diabetes. This study employs both \u003cem\u003ein vitro\u003c/em\u003e and \u003cem\u003ein silico\u003c/em\u003e approaches to assess the antidiabetic efficacy of \u003cem\u003eA. aspera\u003c/em\u003e. In vitro assays were conducted to evaluate the effects of the plant on key enzymes involved in glycemic control and carbohydrate metabolism, namely DPP-4, α-amylase, and α-glucosidase. Significant inhibition of these enzymes was observed, indicating the potential of \u003cem\u003eA. aspera\u003c/em\u003e to regulate blood glucose levels effectively. Additionally, cytotoxicity assays using L6 cell lines demonstrated that \u003cem\u003eA. aspera\u003c/em\u003e extract enhances glucose uptake and remains nontoxic at therapeutic concentrations, suggesting an improvement in insulin sensitivity. Molecular docking studies further supported these findings, which revealed strong interactions between bioactive compounds in \u003cem\u003eA. aspera\u003c/em\u003e and target proteins involved in glucose metabolism. In summary, these results underscore the therapeutic potential of \u003cem\u003eA. aspera\u003c/em\u003e as a natural antidiabetic agent. 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