VP64.14: Comparing 3D and 2D antral follicle count in patients with endometriosis

This study intends to verify whether 3D ultrasound semiautomatic antral follicle count (AFC) is equivalent to 2D real-time AFC to evaluate patients presenting with deep endometriosis and/or endometrioma and to establish the correlation between each method and the number of oocytes retrieved after ovarian stimulation. This was a retrospective cohort study assessing all women with documented diagnosis of deep endometriosis who underwent ovarian stimulation for in vitro fertilisation at a private reproductive medicine centre, between January 2016 and December 2019, who had the AFC exam 3D volume data set stored. The first objective was to compare de differences between the number of oocytes retrieved and the AFC by semiautomatic 3D follicle count and real-time 2D ultrasound count using a t-test. Therefore, we calculated the angular coefficients by Pearson's product-moment to identify the correlation of each AFC with the number of oocytes retrieved. The 3D AFC was obtained using sonography-based automated volume calculation (SonoAVC®), performed by an experienced professional, and the 2D AFC data was collected from the electronic medical record. A total of 36 women had deep endometriosis, documented by magnetic resonance imaging (MRI), laparoscopy or ultrasonography and 3D ovarian volume data set stored from their first exam. The differences between the 2D and 3D AFC and the number of oocytes retrieved at the end of the stimulation were compared, showing no significant statistical difference between both methods (P = 0.59). The angular coefficients were similar using both methods when compared to the number of oocytes retrieved (2D [r = 0.83, CI = 0.68–0.9, P < 0.001]); (3D [r = 0.81, CI = 0.46–0.83, P < 0.001]). This study provides evidence that the 3D semiautomatic AFC possibly can be used to access the ovarian reserve in patients with endometriosis. We believe that the 3D AFC count can be a good alternative to evaluate patients with this condition since it provides a faster image capture, reducing the patient's discomfort.