Differential proteomics analysis of peritoneal macrophages in endometriosis rat treated by Esculentoside A

Objective To evaluate the differential proteomics of the peritoneal macrophages in experimental endometriosis rats treated by EsA.Methods Experimental endometriosis was induced by auto-transplantation of endometrium.Two-dimensional polyacrylamide gel electrophoresis(2D-PAGE) combined with MALDI-TOF/TOF identification were used to investigate the effects of EsA on the proteome of macrophages of experimental endometriosis rats.Results Compared with normal controls,pyruvate kinase3(PKM2).phosphatidylinositol transfer protein(PITPN),and heat shock protein 8(HSP8) had higher expression in peritoneal macrophages of experimental endometriosis,but glyceraldehyde phosphate dehydrogenase(GAPDH) expressed lower.However,these aberrant expression could be inhibited by EsA.Conclusion It was revealed a potential mechanism of the pathogenesis of endometriosis,which gave a new sight to understand the anti-inflammatory effects of EsA and its underlying immunoregulation mechanisms.