Experiment Study on Treating Infertility Patient of Endometriosis with Bushenhuoxue Fang

Objective To observe the effects of Bushenhuoxue Fang on expressions of integrin subunitsβ3 associated with endometrial receptivity and tumor necrotic factor alpha (TNF-α) in endometrial epithelial cells in infertility patient with endometriosis and clinic effects to the infertility patient with endometriosis. Methods Fifty patients were randomly distributed to two groups. Bushenhuoxue Fang were observed (30 cases) and compared with danazol (20 cases). Using immunohistochemical method we detected the expression of integrin subunitsβ3 and TNF-α in endometrial epithelial cells before and after administration of during implantation phase. The results were analysed semi-quantitatively using HSCORE. The significance was determined by Wilcoxon signed rank test. Results During implantation phase, the expressions of integrin subunits β3 in endometrial epithelial cells increased significantly in two group after administration. There were significant different between two groups. The expressions of TNF-α significantly reduced after treatment and no significant difference taken place in the two groups. Conclusion Bushenhuoxue Fang was able to reduce the expression of TNF-α and increase the expressions of integrin subunits β 3 in endometrial epithelial cells of infertility patient with endometriosis during implantation phase. Therefore, they can increse the pregnancy rate of infertility patient with endometriosis through regulating the uteriner receptivity.