BeamMap: Beam Enabled Accurate Mapping and Molecular Analyte Profiling for topography and biochemistry spatial correlation

BeamMap: Beam Enabled Accurate Mapping and Molecular Analyte Profiling for topography and biochemistry spatial correlation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article BeamMap: Beam Enabled Accurate Mapping and Molecular Analyte Profiling for topography and biochemistry spatial correlation Andrei Fedorov, John Sentmanat, Kisurb Choe This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8832802/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted You are reading this latest preprint version Abstract BeamMap is a multimodal bioimaging instrument that integrates scanning electron microscopy with vacuum electrospray-enabled mass spectrometry to enable in situ correlation of biosample structure and untargeted chemistry. It has potential to achieve subcellular topographical imaging alongside chemical mapping via vacuum nanoelectrospray soft ionization with tunable energy for broad range of analyte detection. Demonstrated on a model imaging system, the workflow links morphology and molecular analysis concurrently within the same analytical environment. The BeamMap approach aims to expand discovery-driven single-cell and multiomics investigations across diverse biological samples and tissues. Biological sciences/Biological techniques/Imaging Biological sciences/Biological techniques/Mass spectrometry Full Text Additional Declarations There is NO Competing Interest. Cite Share Download PDF Status: Under Review Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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