Correlation between SCN9A gene polymorphism and ropivacaine for postoperative pain in patients with laparoscopic gynecologic surgery

Objective To analyze the effect of single nucleotide polymorphism(SNP)of SCN9 Agene for the patients with laparoscopic gynecologic operation.Methods A total of 200 patients with laparoscopic gynecologic operation were divided into ropivacaine group(group R)and control group(group C)based on the research method of control and double-blind.5min before cutting the skin,the incision site was locally infiltrated with 12 ml 0.75% ropivacaine(group R)and 12 ml normal saline(group C).The awakening situation after anesthesia and the amount of anesthetic during operation were recorded.By VAS,the pain degree of incision site was assessed and the postoperative adverse reactions were recorded.DNA was extracted from the peripheral venous blood of each patient,and PCR and DNA sequencing were applied to analyze the SNP of SCNA9 gene and the correlation with postoperative pain.Results The mutation(A/C)at 2794 in 15th exon of SCN9 Agene had no correlation with the postoperative pain.The mutation(C/T)at 3448 in 18th exon of SCN9 Agene had correlation with the postoperative pain(P0.05).Conclusion SCN9 Agene exon 18 3448(C/T)heterozygous mutation associates with the occurrence and development of postoperative pain.There is no significant correlation between SCN9 Agene 3448(C/T)mutation and ropivacaine for postoperative pain.