Anticancer Potential of Portunus sanguinolentus Extract Against Cervical Cancer Cells: A Biochemical and Molecular Docking Investigation

Anticancer Potential of Portunus sanguinolentus Extract Against Cervical Cancer Cells: A Biochemical and Molecular Docking Investigation | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Anticancer Potential of Portunus sanguinolentus Extract Against Cervical Cancer Cells: A Biochemical and Molecular Docking Investigation Rakeshwaran Anbarasu, Deepalakshmi K, Kavitha S This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-7995748/v1 This work is licensed under a CC BY 4.0 License Status: Under Revision Version 1 posted 10 You are reading this latest preprint version Abstract Introduction Cervical cancer, primarily caused by human papillomavirus (HPV), remains a significant health burden, with squamous cell carcinoma (SCC) accounting for 70–80% of cases, often linked to high-risk HPV types 16 and 18. Current treatments like surgery, radiation, and chemotherapy have limitations, driving the search for targeted therapies. This study explores the anticancer potential of Portunus sanguinolentus (crab shell) extract on HeLa cells, focusing on its bioactive compounds, antioxidant properties, and ability to inhibit cancer cell growth. These findings provide a foundation for developing novel therapeutic strategies for cervical cancer. Material and Methods In the study crab shells were collected and powdered for ethanol extraction. Bioactive compounds were screened via biochemical analysis & GC-MS. Molecular docking was done to reveal γ-sitosterol binding with RPA receptors. Antioxidant properties were evaluated using a DPPH assay. Cytotoxic effects on HeLa and liver cells were assessed through MTT assays, demonstrating concentration-dependent HeLa suppression with minimal toxicity to normal liver cells. Result Analysis identified glycosides, alkaloids, and steroids, with γ-sitosterol showing the longest retention time. γ-Sitosterol exhibited significant anticancer properties. Molecular docking revealed its strong binding affinity with the RPA receptor. Cytotoxicity studies showed moderate HeLa cell suppression in a concentration-dependent manner through the MTT assay, while exhibiting minimal toxicity to liver cells, highlighting the extract’s potential as an anticancer agent. Conclusion The findings highlight the biochemical makeup and molecular interactions of Portunus sanguinolentus extract's remarkable therapeutic potential against cervical cancer, indicating that it should be further studied for cancer therapy procedures. Cervical cancer γ-sitosterol Hela cell Crab shell extract Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Under Revision Version 1 posted Editorial decision: Revision requested 21 Nov, 2025 Reviews received at journal 13 Nov, 2025 Reviews received at journal 12 Nov, 2025 Reviewers agreed at journal 06 Nov, 2025 Reviewers agreed at journal 04 Nov, 2025 Reviewers agreed at journal 04 Nov, 2025 Reviewers invited by journal 04 Nov, 2025 Editor assigned by journal 31 Oct, 2025 Submission checks completed at journal 31 Oct, 2025 First submitted to journal 31 Oct, 2025 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-7995748","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":544758051,"identity":"1abcc470-3884-40ee-8541-e2e3adcd0307","order_by":0,"name":"Rakeshwaran Anbarasu","email":"","orcid":"","institution":"Indian Institute of Technology","correspondingAuthor":false,"prefix":"","firstName":"Rakeshwaran","middleName":"","lastName":"Anbarasu","suffix":""},{"id":544758053,"identity":"6a97b106-464d-4835-9905-0c57400243a3","order_by":1,"name":"Deepalakshmi K","email":"","orcid":"","institution":"Prince Shri Venkateshwara Arts and Science 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Current treatments like surgery, radiation, and chemotherapy have limitations, driving the search for targeted therapies. This study explores the anticancer potential of \u003cem\u003ePortunus sanguinolentus\u003c/em\u003e (crab shell) extract on HeLa cells, focusing on its bioactive compounds, antioxidant properties, and ability to inhibit cancer cell growth. These findings provide a foundation for developing novel therapeutic strategies for cervical cancer.\u003c/p\u003e\u003ch2\u003eMaterial and Methods\u003c/h2\u003e\u003cp\u003eIn the study crab shells were collected and powdered for ethanol extraction. Bioactive compounds were screened via biochemical analysis \u0026amp; GC-MS. Molecular docking was done to reveal γ-sitosterol binding with RPA receptors. Antioxidant properties were evaluated using a DPPH assay. Cytotoxic effects on HeLa and liver cells were assessed through MTT assays, demonstrating concentration-dependent HeLa suppression with minimal toxicity to normal liver cells.\u003c/p\u003e\u003ch2\u003eResult\u003c/h2\u003e\u003cp\u003eAnalysis identified glycosides, alkaloids, and steroids, with γ-sitosterol showing the longest retention time. γ-Sitosterol exhibited significant anticancer properties. Molecular docking revealed its strong binding affinity with the RPA receptor. Cytotoxicity studies showed moderate HeLa cell suppression in a concentration-dependent manner through the MTT assay, while exhibiting minimal toxicity to liver cells, highlighting the extract\u0026rsquo;s potential as an anticancer agent.\u003c/p\u003e\u003ch2\u003eConclusion\u003c/h2\u003e\u003cp\u003eThe findings highlight the biochemical makeup and molecular interactions of \u003cem\u003ePortunus sanguinolentus\u003c/em\u003e extract's remarkable therapeutic potential against cervical cancer, indicating that it should be further studied for cancer therapy procedures.\u003c/p\u003e","manuscriptTitle":"Anticancer Potential of Portunus sanguinolentus Extract Against Cervical Cancer Cells: A Biochemical and Molecular Docking Investigation","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-11-14 12:26:38","doi":"10.21203/rs.3.rs-7995748/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2025-11-21T19:34:07+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-13T21:55:58+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2025-11-12T15:35:50+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"105413309587873178126516698196040917532","date":"2025-11-06T15:12:51+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"324705903904476076308544599427402001455","date":"2025-11-05T03:22:55+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"182290908216465658348489228362412091269","date":"2025-11-05T00:31:37+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2025-11-04T22:58:27+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2025-10-31T08:58:26+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2025-10-31T08:57:36+00:00","index":"","fulltext":""},{"type":"submitted","content":"In Silico Pharmacology","date":"2025-10-31T07:27:15+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"in-silico-pharmacology","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"insp","sideBox":"Learn more about [In Silico Pharmacology](https://link.springer.com/journal/40203)","snPcode":"40203","submissionUrl":"https://submission.nature.com/new-submission/40203/3","title":"In Silico Pharmacology","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"4581bfd6-830c-45e2-9a16-cf3cdae0497f","owner":[],"postedDate":"November 14th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"in-revision","subjectAreas":[],"tags":[],"updatedAt":"2026-03-27T14:56:36+00:00","versionOfRecord":[],"versionCreatedAt":"2025-11-14 12:26:38","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-7995748","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-7995748","identity":"rs-7995748","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}