Isolation and characterization of forty-two polymorphic simple sequence repeat markers from Mastacembelus armatus

Abstract A total of 42 polymorphic simple sequence repeat (SSR) markers have been developed and characterized from genome sequences of Mastacembelus armatus to safeguard its natural resources. Polymorphisms of these SSR markers were evaluated in a natural population of 35 individuals. The number of alleles per locus ranged from two to fourteen, observed heterozygosity and expected heterozygosity varied from 0.031 to 0.813, and from 0.091 to 0.852, respectively. Three SSR loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These loci are the first time to be characterized and will be useful for genetic conservation and marker-assisted breeding for M. armatus.