Moringa Dried Leaf Extract as Bio-Foliar Fertilizer for Revitalizing Performance and Nutritional Status of Soybean

preprint OA: closed
Full text JSON View at publisher

Abstract

Abstract Naturally occurring plant based biostimulants can be used to improve crop productivity and quality as an eco-friendly approach. Moringa oleifera, known as the miracle tree, is a rich source of essential nutrients and beneficial compounds that can act as potent growth enhancer. The current work aimed to appraise whether moringa dried leaf extract (MDLE) could improve growth, physiology, seed nutritional and quality attributes of soybean. The designed study followed a completely randomized design (CRD) with factorial arrangement having four replications examining various MDLE concentrations [water spray as control, 0.5%, 1.0%, and 1.5% (w/v)] and growth stages (vegetative and reproductive) of soybean. The results revealed that foliar application of 1.5% MDLE during the reproductive stage of soybean showcased remarkable enhancements. Notably, seedling growth metrics, photosynthetic pigments, leaf area, gas exchange parameters, yield attributes, seed nutritional and quality attributes were significantly boosted. Overall, a noteworthy increase in seedling growth metrics (22%), carotenoids (8.33%), gas exchange attributes (18%), yield components (37%) and seed nutritional attributes (18%) was observed with 1.5% MDLE application during the reproductive stage. This underscores the potential of MDLE foliar application during reproductive stage in improving soybean growth, quality and seed nutritional contents.
Full text 15,375 characters · extracted from preprint-html · click to expand
Moringa Dried Leaf Extract as Bio-Foliar Fertilizer for Revitalizing Performance and Nutritional Status of Soybean | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Article Moringa Dried Leaf Extract as Bio-Foliar Fertilizer for Revitalizing Performance and Nutritional Status of Soybean Sohail Irshad, Amar Matloob, Kashf Mehmood, Muhammad Nawaz, Shahid Iqbal, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4862579/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 01 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted 10 You are reading this latest preprint version Abstract Naturally occurring plant based biostimulants can be used to improve crop productivity and quality as an eco-friendly approach. Moringa oleifera, known as the miracle tree, is a rich source of essential nutrients and beneficial compounds that can act as potent growth enhancer. The current work aimed to appraise whether moringa dried leaf extract (MDLE) could improve growth, physiology, seed nutritional and quality attributes of soybean. The designed study followed a completely randomized design (CRD) with factorial arrangement having four replications examining various MDLE concentrations [water spray as control, 0.5%, 1.0%, and 1.5% (w/v)] and growth stages (vegetative and reproductive) of soybean. The results revealed that foliar application of 1.5% MDLE during the reproductive stage of soybean showcased remarkable enhancements. Notably, seedling growth metrics, photosynthetic pigments, leaf area, gas exchange parameters, yield attributes, seed nutritional and quality attributes were significantly boosted. Overall, a noteworthy increase in seedling growth metrics (22%), carotenoids (8.33%), gas exchange attributes (18%), yield components (37%) and seed nutritional attributes (18%) was observed with 1.5% MDLE application during the reproductive stage. This underscores the potential of MDLE foliar application during reproductive stage in improving soybean growth, quality and seed nutritional contents. Biological sciences/Plant sciences Biological sciences/Plant sciences/Plant physiology Biological sciences/Plant sciences/Plant stress responses Biostimulants gas exchange attributes growth stages moringa dried leaf extract seed nutritional attributes Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 01 Jul, 2025 Read the published version in Scientific Reports → Version 1 posted Editorial decision: Revision requested 03 Oct, 2024 Reviews received at journal 18 Sep, 2024 Reviewers agreed at journal 16 Sep, 2024 Reviews received at journal 23 Aug, 2024 Reviewers agreed at journal 22 Aug, 2024 Reviewers invited by journal 20 Aug, 2024 Editor assigned by journal 20 Aug, 2024 Editor invited by journal 05 Aug, 2024 Submission checks completed at journal 05 Aug, 2024 First submitted to journal 05 Aug, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4862579","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Article","associatedPublications":[],"authors":[{"id":346686463,"identity":"85f9e5b8-bf97-47bf-abe1-c5d94329e890","order_by":0,"name":"Sohail Irshad","email":"","orcid":"","institution":"MNS-University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Sohail","middleName":"","lastName":"Irshad","suffix":""},{"id":346686466,"identity":"5042c1ba-be14-4742-8038-45d9a802f2a3","order_by":1,"name":"Amar Matloob","email":"","orcid":"","institution":"MNS-University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Amar","middleName":"","lastName":"Matloob","suffix":""},{"id":346686469,"identity":"2701d651-f658-49a0-aebf-23aeeb65858f","order_by":2,"name":"Kashf Mehmood","email":"","orcid":"","institution":"Superior University","correspondingAuthor":false,"prefix":"","firstName":"Kashf","middleName":"","lastName":"Mehmood","suffix":""},{"id":346686470,"identity":"af89e3dd-6773-4416-84b7-0cb011b2a928","order_by":3,"name":"Muhammad Nawaz","email":"","orcid":"","institution":"Khwaja Fareed University of Engineering and Information Technology","correspondingAuthor":false,"prefix":"","firstName":"Muhammad","middleName":"","lastName":"Nawaz","suffix":""},{"id":346686473,"identity":"ef555df5-1ac1-4820-a8bf-3ca80c15c491","order_by":4,"name":"Shahid Iqbal","email":"","orcid":"","institution":"MNS-University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Shahid","middleName":"","lastName":"Iqbal","suffix":""},{"id":346686474,"identity":"1d31a316-7726-4caf-b2e1-6ec957db7380","order_by":5,"name":"Muhammad Ashfaq Wahid","email":"","orcid":"","institution":"University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Muhammad","middleName":"Ashfaq","lastName":"Wahid","suffix":""},{"id":346686475,"identity":"6a186125-a4d7-407d-8330-1d45a3c914b2","order_by":6,"name":"Rao Muhammad Ikram","email":"","orcid":"","institution":"MNS-University of Agriculture","correspondingAuthor":false,"prefix":"","firstName":"Rao","middleName":"Muhammad","lastName":"Ikram","suffix":""},{"id":346686476,"identity":"20409d70-8353-4c73-89fc-6f8948202189","order_by":7,"name":"Muhammad Atif Ghafoor","email":"","orcid":"","institution":"Soil and Water Testing Laboratory","correspondingAuthor":false,"prefix":"","firstName":"Muhammad","middleName":"Atif","lastName":"Ghafoor","suffix":""},{"id":346686477,"identity":"771ffffa-697c-4dc2-bc04-03e7af860de9","order_by":8,"name":"Saud Alamri","email":"","orcid":"","institution":"King Saud University","correspondingAuthor":false,"prefix":"","firstName":"Saud","middleName":"","lastName":"Alamri","suffix":""},{"id":346686478,"identity":"725f0315-9ff9-4ac9-8c45-1e916c9aa390","order_by":9,"name":"Shahbaz Khan","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAABCklEQVRIie2PMWvCQBiGXznIdDXrF06Sv5AiRAv5MSlCXVJwEodghYJTcY74Jzo55xDSJeB6Q4eC0FkoiJP00gqF0pOMHe4Z7l7uvgfeD7BY/iMEh33dpI890Pn5KhoorRzgADtPN1EYb6IEq8f33SibwqtDnMXcdbfyYz+B31bJn0r4Wva6eelAdHRIyzvu5QNGRYWuZ1IoiQR3OPw63M82PFQMJOe4fTYoQT48CH4irehwUyvbDTvKEx5MClQaiat5CEE6tGqlGDgkZ0hCUzGVjsVykegV0rH39L1L1K9Kul5Wb6ZiazE6TH1SwzUds9h3XblTkywO2i+GYmf47we6OG6xWCyWy3wCyhxYBrsXRa0AAAAASUVORK5CYII=","orcid":"","institution":"Colorado State University","correspondingAuthor":true,"prefix":"","firstName":"Shahbaz","middleName":"","lastName":"Khan","suffix":""}],"badges":[],"createdAt":"2024-08-05 14:09:06","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4862579/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4862579/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1038/s41598-025-95404-0","type":"published","date":"2025-07-01T15:58:27+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":86180289,"identity":"7bd7fdd9-37f9-4c05-8244-701a21e34a10","added_by":"auto","created_at":"2025-07-07 16:21:58","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":552713,"visible":true,"origin":"","legend":"","description":"","filename":"ManuscriptSR.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4862579/v1_covered_241b26a1-0067-4f76-9ccd-77aaa4e68824.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Moringa Dried Leaf Extract as Bio-Foliar Fertilizer for Revitalizing Performance and Nutritional Status of Soybean","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"Biostimulants, gas exchange attributes, growth stages, moringa dried leaf extract, seed nutritional attributes","lastPublishedDoi":"10.21203/rs.3.rs-4862579/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4862579/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Naturally occurring plant based biostimulants can be used to improve crop productivity and quality as an eco-friendly approach. Moringa oleifera, known as the miracle tree, is a rich source of essential nutrients and beneficial compounds that can act as potent growth enhancer. The current work aimed to appraise whether moringa dried leaf extract (MDLE) could improve growth, physiology, seed nutritional and quality attributes of soybean. The designed study followed a completely randomized design (CRD) with factorial arrangement having four replications examining various MDLE concentrations [water spray as control, 0.5%, 1.0%, and 1.5% (w/v)] and growth stages (vegetative and reproductive) of soybean. The results revealed that foliar application of 1.5% MDLE during the reproductive stage of soybean showcased remarkable enhancements. Notably, seedling growth metrics, photosynthetic pigments, leaf area, gas exchange parameters, yield attributes, seed nutritional and quality attributes were significantly boosted. Overall, a noteworthy increase in seedling growth metrics (22%), carotenoids (8.33%), gas exchange attributes (18%), yield components (37%) and seed nutritional attributes (18%) was observed with 1.5% MDLE application during the reproductive stage. This underscores the potential of MDLE foliar application during reproductive stage in improving soybean growth, quality and seed nutritional contents.","manuscriptTitle":"Moringa Dried Leaf Extract as Bio-Foliar Fertilizer for Revitalizing Performance and Nutritional Status of Soybean","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-08-30 05:33:57","doi":"10.21203/rs.3.rs-4862579/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-10-03T17:33:10+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-09-18T10:54:31+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"42706839861233866969291955876000090297","date":"2024-09-16T06:54:34+00:00","index":"hide","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-08-23T12:16:45+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"5265701220372325339023922671935844789","date":"2024-08-22T16:28:32+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-08-20T14:17:25+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-08-20T13:54:24+00:00","index":"","fulltext":""},{"type":"editorInvited","content":"","date":"2024-08-05T14:30:09+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2024-08-05T14:25:48+00:00","index":"","fulltext":""},{"type":"submitted","content":"Scientific Reports","date":"2024-08-05T14:07:48+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"1762b908-61c4-417b-91ac-b7da674900a1","owner":[],"postedDate":"August 30th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[{"id":36763780,"name":"Biological sciences/Plant sciences"},{"id":36763781,"name":"Biological sciences/Plant sciences/Plant physiology"},{"id":36763782,"name":"Biological sciences/Plant sciences/Plant stress responses"}],"tags":[],"updatedAt":"2025-07-07T16:17:19+00:00","versionOfRecord":{"articleIdentity":"rs-4862579","link":"https://doi.org/10.1038/s41598-025-95404-0","journal":{"identity":"scientific-reports","isVorOnly":false,"title":"Scientific Reports"},"publishedOn":"2025-07-01 15:58:27","publishedOnDateReadable":"July 1st, 2025"},"versionCreatedAt":"2024-08-30 05:33:57","video":"","vorDoi":"10.1038/s41598-025-95404-0","vorDoiUrl":"https://doi.org/10.1038/s41598-025-95404-0","workflowStages":[]},"version":"v1","identity":"rs-4862579","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-4862579","identity":"rs-4862579","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}

Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.

My notes (saved in your browser only)

Ask this paper AI returns verbatim quotes from the full text · source: preprint-html

Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works

Citation neighborhood (no data yet)

We don't have any in-corpus citations linked to this paper yet. This is a recent paper (2024) — citers typically take a year or two to land, and the OpenAlex reference graph may still be filling in.

Source provenance

europepmc
last seen: 2026-05-20T01:45:00.602351+00:00