Effect of Herbs Softening and Resolving Masses on the Adhesion Factors of Endometrium of Endometriesis Model Mice

Objective To evaluate the effect of herbal medicines with the functions to soften and resolve masses of ectopic endometrium of mouse model of endometriosis.Methods The enhanced green fluorescent protein (EGFP) transgenic and wild-type C57BL/6J mice were used to establish the mouse model of endometriosis.For the herb group,the herbal medicine was administered by gavage.For the control and blank groups,the same amount saline was given by gavage.Two weeks later,all model mice were sacrificed and optical density of fluorescence of ectopic endometrium was calculated for comparison.The ectopic endometrium,uterus and ovaries were examined histologically and FCM analysis was performed to access cell apoptosis,Western blot and Real-time PCR was performed to access the expression of ICAM-1 and CD44 in the ectopic endometrium.Results Under fluorescent microscope,the green cake-like or punctiform ectopic endometrium were found between the intestines,under abdominal wall or under hepatic lobules or around spleen in the model mice.The volume and optical density of fluorescence of ectopic endometrium of the herb group were significantly lower than those of the control group(P 0.05),the cell apoptosis of the herb group and the control group was significantly different from that of the blank group(P 0.01),and the herb group was also higher than the control group(P0.05).The expression of CD44,ICAM-1 and mRNA in the herb group was significantly lower than that of the control group(P 0.01).The expression of CD44,ICAM-1 and mRNA of the control and the herb group was 16.34 and 2.75,and 1.53 and 1.19 times respectively of the blank group.Conclusion The herbs with the functions to soften and resolve masses exert a growth-inhibitory effect on ectopic endometrium of model mice.It is may be realized by inhibiting the expression of CD44 and ICAM-1.