Production of Iron-enriched Yeast and it’s application in the treatment of iron-deficiency anemia

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After the model was established, IDA mice were randomly divided into 5 groups: normal control group, IDA group, organic iron (Fe-Gly) group, inorganic iron (FeSO4) group and yeast iron (Fe-F8) group.Mice in the experimental group were given different kinds of iron by intragastric administration once a day for 4w.The results showed that Fe-F8 had an effective recovery function, and the body weight and hematological parameters of IDA mice returned to normal levels. The activities of malondialdehyde, superoxide dismutase, glutathione peroxidase and total antioxidant capacity in serum were increased.In addition, Fe-F8 was more effective in alleviating IDA and improving organ indices with fewer side effects compared to ferrous glycinate and ferrous sulfate groups. This study suggests that the iron-rich strain F8 may play an important role in improving IDA mice and may be developed as a new iron supplement. Iron deficiency anemia Mice Ferriferous yeast Hematological parameters Antioxidant activity Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 1. Introduction Iron is an essential trace element for most life forms and is involved in many biological processes [1] . Iron deficiency is one of the most common nutritional deficiencies worldwide. Severe iron deficiency can cause iron deficiency anemia (IDA), and iron deficiency can impair immune response [2] , physical work ability and intellectual function [3] . Iron ion is the second most abundant metal element in the Earth's crust, but iron deficiency, especially iron deficiency anemia, is a common nutrient deficiency in humans, affecting about 1.6 billion people worldwide [4] . Iron plays a crucial role in the normal development of the central nervous system, which can affect myelin formation, neuronal and glial energy metabolism, and neurotransmitter production [5] . Iron deficiency during the critical period of development will have irreversible harmful effects on the brain. Lead to cognitive and behavioral abnormalities [6] . To improve iron deficiency anemia, several forms of iron supplements have been developed over the past two decades. At present, the research on iron supplements is more concerned about the blood-replenishing activity of iron supplements, and the side effects of iron supplements such as intestinal inflammation are still lacking. At present, oral iron supplements used in clinical practice are mostly traditional iron supplements, such as FeSO 4 , ferrous succinate, ferrous fumarate, ferrous gluconate, ferrous glycine, etc., but they all have iron odor, instability, low bioavailability, and are prone to dietary taboo, free iron toxicity, gastrointestinal discomfort and other symptoms [7] . FeSO 4 was first used in clinical treatment, easily soluble in water, good bioavailability, low cost, is the most widely used oral iron agent. However, its bioavailability is low, accompanied by side effects such as stomach pain and diarrhea, and it is easy to cause lipid peroxidation and even DNA damage [8–9] FeSO 4 has high iron content and good absorption effect, which may help alleviate the symptoms of iron deficiency and anemia to a certain extent. But it can cause many adverse reactions, such as diarrhea and constipation [10] . Other common inorganic iron agents include FeCl 3 ·6H 2 O, FeCO 3 , FeNaPO 4 and Fe 2 P 2 O 7 . Organic ferric agents are mainly some small molecular organic acid ferric salts, such as ferrous fumarate (43.4%), ferric albuminate (7%) and Fe-Gly [11] . Studies have shown that Fe-Gly iron is better absorbed than FeSO4 iron [12] .However, recently, many new iron supplements have appeared, such as the chelation of iron with peptides derived from proteins in hydrolyzed foods, and the conversion of iron into organic iron by cells [13–14] . There are also some new macromolecular complexes such as polypeptide iron chelates, heme iron, polysaccharide iron, iron-rich yeast, etc. These have caused great concern. Among them, iron-rich yeast has the characteristics of high absorption rate, low irritation and rich nutrition. Inorganic iron can be converted into organic form through the yeast reproduction process, which makes iron more easily absorbed by the body. It is a good new iron supplement for preventing and treating IDA.Studies have shown that iron-rich bread yeast can improve the levels of red blood cells (RBC) and HGB in anemic mice, and can alleviate the damage caused by anemia to various organs of the body [15] . The form of iron in iron-rich yeast is similar to that in human body, so iron-rich yeast does not have iron toxicity caused by free iron ion enrichment. Studies have shown that compared with FeSO4 and amino acid chelated iron, iron-rich yeast has a higher bioavailability, showing a good effect on IDA treatment [16] . In conclusion, the treatment of IDA should increase iron intake and iron absorption and availability, reduce intestinal adverse reactions as much as possible, and thus improve the effectiveness of treatment and patient compliance. Therefore, the aim of this study was to investigate the improvement of yeast iron (Fe-F8) on iron deficiency anemia mice. The effects of yeast iron(Fe-F8) on hematological parameters and organs of mice were studied. In addition, the antioxidant effects of Fe-F8 on anemic mice were evaluated to investigate whether Fe-F8 is promising for development as a new iron supplement. 2. Materials and Methods Preparation of iron-rich yeast, F8 and cell iron content evaluation (1) Test tube culture seed solution Strain Fe-F8 was immersed in a test tube containing 10 mL YPD medium (2% glucose, 1% yeast powder, 2% tryptone) at 30℃ and 200 rpm for 18 h. Then, it was inoculated in 250 mL flask of 50 mL YPD medium at a ratio of 2% (v/v) at 30℃, 200 rpm, and oscillated for 18 h as seed liquid for reserve. (2) Flask culture The strain Fe-F8 seed liquid was inoculated in 250 mL shaking bottle with 50 mL YPD medium at 30℃ and 200 rpm at a ratio of 2% (v/v), and oscillated for 18 h as secondary seed liquid. (3) Fermentation culture The secondary seed solution was added to the fermentation medium (molasses 2%, (NH4) 2SO4 0.6%, KH2PO4 1.0%, pH 5.1) at 10% (v/v). Add iron salt before inoculation, take 36 mL of 1.5L fermentation solution and add it into culture solution. The fermentation time was 28 h. Fermentation temperature 30℃, rotational speed: 0–2 h: 200 rpm; 2–5 h: 300 rpm; 5–8 h: 400 rpm; 8–10 h: 500 rpm; 10–12 h: 600 rpm; After 12 hours: 700 rpm; If the dissolved oxygen is less than 30%, the speed is adjusted to 800 rpm. Maintain fermentation pH at 4.4 with 20% NaOH solution. The dissolved oxygen is controlled at 50%-60%. After fermentation was completed, the ferrified yeast cells with a dry weight of about 0.1g were weighed into a digestion bottle, and 5 mL of digestion liquid (HNO3:HClO4 = 5:1) was added to the heating plate at 200℃ for digestion until the liquid was clear and transparent. Then, the cells were removed and cooled, and the volume of distilled water was fixed to 25 mL, and 1 mL of constant volume liquid was absorbed and 4 mL of distilled water was added. After mixing well, absorb 200 µL into 7 ml EP tube, add 3.0 mL distilled water, add 0.1 mL 1M hydrochloric acid solution, 0.1 mL 10% hydroxylamine hydrochloride, 0.1 mL 0.12% o-diazophene (1, 10-phenanthroline), add 0.5 mL 10% sodium acetate, shake well. The content of cell iron in ferriferous yeast was calculated at 510 nm by using the absorbance value of the blank solution without ferriferous agent as reference solution. Animals and experimental design Animals The 3-week-old healthy male KM mice were from Beijing Weitonglihua Laboratory Animal Technology Co., LTD. According to the requirements of the National Act on the use of laboratory animals (People's Republic of China), all animals are used in the current research after the evaluation and accreditation of the Association for the Care of Laboratory Animals (AAALAC) and the protocol for Animal testing is approved by the Animal Ethics Committee. All mice were housed in stainless steel cages with sawdust pads and kept in an environmentally controlled room (24 ± 2°C and 50% ± 10% relative humidity) for a 12-hour light/dark cycle. The sawdust mat is renewed every 3 days and the rats have free access to food and water. Experimental design After 3 days of adaptation, 60 3-week-old male KM mice were randomly divided into normal control group (n = 12) and model group (n = 48). The normal control group was fed the standard diet of AIN-93G throughout the experiment. The model group was fed AIN-93G diet, containing 8 mg iron per kg diet, TROPHIC Animal Feed. High-tech Co., Ltd,Nantong, China) induced IDA model. The whole experiment process was strictly controlled, iron staining was avoided, and hemoglobin (Hb) levels were analyzed weekly. After 4 weeks, IDA with Hb content less than 90 g/L was taken as IDA, and then recovery experiment was carried out. After IDA mouse model was established, IDA mice (n = 48) were randomly divided into 4 groups with 12 mice in each group. Hb concentration was balanced. IDA iron deficiency model group was given the above iron deficiency diet. Organic iron group was given 3.0mg Fe/kg BWFe-Gly; The inorganic iron group was given 3.0mg Fe/kg BW FeSO4, and the sample group was given 3.0mg Fe/kg BW yeast iron Fe-F8. Fe-Gly, FeSO4 and Fe-F8 were dissolved in distilled water, and the mice were given the above dosage by intragastric administration. The normal control group and IDA model group were given the same volume of deionized water. All supplements were prepared fresh before use and administered by gavage at 10 am daily for 4 weeks. During this time, weigh yourself once a week. Sample collection At the end of the whole experiment, the weight of the mice was measured after fasting for 12 hours, the mice were dissected, and blood was collected and placed in the heparin sodium anticoagulant tube. The collected samples were centrifuged to obtain plasma and serum and frozen at − 20°C for further analysis. All mice, heart, liver, stomach, kidney, spleen, and colon, were rinsed with normal saline, weighed, and stored at − 80°C for further analysis. Hematological test The hemoglobin content (Hb), red blood cell count (RBC) and hematocrit (HCT) in the blood were measured by the kit. HCT is expressed as the percentage of red blood cells in total blood volume. Serum iron (SI) concentration was measured using the SI test kit (Beijing Solebol Technology Co., LTD., Beijing, China) according to the manufacturer's instructions. Organ coefficient Pathological examination of mice was performed by naked eye during dissection. All mice had their hearts, livers, stomachs, kidneys, spleens and colons removed and weighed. The relative weight of each organ is calculated based on the final weight measured that day. The organ coefficient is calculated as follows: Determination of antioxidant enzyme activity and malondialdehyde level and total antioxidant capacity Serum glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) were measured using a diagnostic kit (Beijing Solaibo Technology Co., LTD., Beijing, China) according to the manufacturer's instructions. Statistical analysis Data are expressed as mean ± SD and analyzed by one-way analysis of variance (ANOVA) and Duncan’s multiple range tests using SPSS 17.0 software. P < 0.05 was considered statistically significant. 3. Results 3.1. Preparation and determination of iron content in yeast Table 1 Screening results of iron-rich yeast. Strain CDM medium Molasses culture medium Biomass (g/L) Cell iron content (mg/g) Biomass (g/L) Cell iron content (mg/g) F1 8.6 ± 0.1 1.05 ± 0.03 9.1 ± 0.1 0.99 ± 0.05 F2 9.0 ± 0.1 1.21 ± 0.04 10.1 ± 0.1 1.06 ± 0.04 F3 8.4 ± 0.1 1.46 ± 0.03 9.7 ± 0.4 1.31 ± 0.05 F4 8.7 ± 0.1 1.10 ± 0.03 9.9 ± 0.3 1.03 ± 0.05 F5 7.8 ± 0.1 1.35 ± 0.03 9.6 ± 0.1 1.30 ± 0.06 F6 9.6 ± 0.2 1.15 ± 0.05 11.8 ± 0.3 0.99 ± 0.03 F7 6.8 ± 0.1 1.32 ± 0.04 8.5 ± 0.1 1.27 ± 0.05 F8 11.2 ± 0.3 1.62 ± 0.06 13.0 ± 0.3 1.49 ± 0.06 F9 7.1 ± 0.1 1.16 ± 0.05 8.9 ± 0.1 1.09 ± 0.04 * Strain no. F1-F9 was obtained from lab-preserved strains. Molasses medium: molasses 2%, (NH 4 ) 2 SO 4 0.6%, KH 2 PO 4 1.0%, FeSO 4 ·7H 2 O 5mM, pH 5.1; CDM medium: Pancreatic digest of casein 1.7%, Papaic digest of soybean meal 0.3%, Sodium choride 0.5%, Dipotassium hydrogen phosphate 0.25%, C 6 H 12 O 6 ·H 2 O 0.25%, pH 7.3. Strain no. F8 was selected according to its biomass and enrichment rate of iron ions. Studies have shown that iron ion can inhibit the growth of microorganisms[ 17 ], so in order to obtain strains with high iron and high biomass, it is necessary to select the best concentration of iron ion in the medium. As shown in Table 1 , Strain no. F8 has its unique growth characteristics, with high biomass and cell iron content in molasses medium. Although the iron-rich strains in CDM medium had higher cell iron content, their biomass was low, and CDM medium was not suitable for large-scale fermentation due to its complex composition, cumbersome configuration and high cost, so molasses medium was used for further optimization. Furthermore, the effects of adding FeSO4·7H2O or triferric citrate in molasses medium with different concentration gradients on cell growth and iron enrichment of Fe-rich strain F8 were investigated. Table 2 Iron enrichment results of iron-rich yeast F8 in molasses medium with different iron concentrations. Iron concentration(mM) Biomass (g/L) Cell iron content (mg/g) FeSO4 Ferric citrate FeSO4 Ferric citrate 2 9.53 ± 0.46 10.39 ± 0.19 1.83 ± 1.75 0.78 ± 0.29 4 10.56 ± 0.17 10.99 ± 0.14 6.69 ± 5.61 2.80 ± 0.08 6 11.87 ± 0.24 10.64 ± 0.14 12.75 ± 3.66 6.82 ± 0.07 8 11.69 ± 0.37 10.84 ± 0.02 16.64 ± 0.61 9.50 ± 0.16 10 11.90 ± 0.14 11.30 ± 0.24 19.96 ± 4.05 12.37 ± 0.65 12 12.31 ± 0.53 12.30 ± 0.39 21.10 ± 7.20 16.35 ± 0.46 The experimental results in Table 2 show that the biomass of iron-rich strains supplemented with FeSO4·7H2O and triferric citrate has little difference, but the iron content of cells supplemented with FeSO4·7H2O is higher than that of cells supplemented with triferric citrate. Strain no. F8 can grow in a high iron environment. Finally, The highest biomass and cell iron content were obtained in the medium with 12 mM FeSO4·7H2O concentration. In this study, the genome of iron-rich Strain no. F8 was extracted, ITS sequence was amplified, and the strain was identified as Saccharomyces cerevisiae by re-comparison in the database. Yeast is a good carrier of trace elements, which can enrich many trace elements including iron. By means of fermentation technology, adding an appropriate amount of iron ion to the medium can make yeast cells absorb a large amount of iron ion during the proliferation process and bind it to the organic parts of the cells, thus obtaining iron-rich yeast[ 18 ]. Relevant studies on iron-rich yeasts have been carried out both at home and abroad, and most of them used Saccharomyces cerevisiae as the carrier of iron enrichment[ 19 ],In addition, other researchers have used Rhodotorula glutinis[ 20 ]、Candida intermedia、Kluyveromyces marxianus[ 21 ] and so on. Iron-rich yeast can convert inorganic sources of iron into low-toxicity, bioavailable organic iron, which is more beneficial to the human body and has higher biological activity, and is suitable as a new safe iron supplement for the prevention and treatment of iron deficiency anemia[ 19 ]. In addition, yeast is rich in amino acids, proteins, vitamins and other nutrients[ 21 ], and can also be used as a nutritional element supplement, energy and immune enhancer in addition to iron supplementation[ 22 ]. 3.2. Body weight change in mice Table 3 Effects of different treatments on survival rate of mice. Normal control group IDA group Fe-Gly group FeSO4 group Fe-F8 group Death 0 0 1 5 0 Survival rate(%) 100% 100% 92% 58% 100% * The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO 4 group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively. Figure 1 shows the growth of mice in each group. There was a large difference in weight gain. The feed intake of the model group was high but the weight of the model group increased more slowly than that of the normal control group, indicating that iron deficiency affected the normal growth of mice. Zheng's study also obtained similar results[ 23 ].The yeast group was able to gain weight significantly, which was comparable to the normal control group. However, the overall weight gain trend decreased, which may be due to the small stress response of mice in the early stage, and the weight of mice in the late stage also tended to be saturated. Among them, Fe-Gly group and FeSO4 group lost weight in the later period, and the ratio of feed to gain was significantly higher than other groups.Table 3 survival rate showed that the FeSO4 group died the most, 5 mice in total, and more lesions were found in their organs after dissection. Other studies have also shown that FeSO4 has certain toxicity, which may be detrimental to mouse growth[ 10 ]. Many studies on IDA have shown that IDA affects growth in rats, so our results show that these findings are consistent with previous reports[ 24 ]. 3.3. Changes of organ coefficient in mice Table 4 Organ coefficient in the heart, liver, stomach, kidney and spleen of mice in different groups. Group Organ coefficient Heart Liver Stomach Kidney Spleen Normal control group 0.53 ± 0.06 d 5.31 ± 0.41 a 1.57 ± 0.48 c 1.66 ± 0.29 a 0.29 ± 0.07 ab IDA group 0.59 ± 0.08 a 5.03 ± 0.42 a 1.83 ± 0.13 b 1.64 ± 0.18 a 0.31 ± 0.08 a Fe-Gly group 0.56 ± 0.01 c 5.01 ± 0.39 c 2.15 ± 0.06 a 1.51 ± 0.19 a 0.26 ± 0.06 c FeSO4 group 0.55 ± 0.08 c 4.79 ± 0.40 d 2.13 ± 0.36 a 1.51 ± 0.09 a 0.19 ± 0.05 d Fe-F8 group 0.57 ± 0.11 b 5.19 ± 0.59 b 1.70 ± 0.69 bc 1.58 ± 0.21 a 0.28 ± 0.06 bc * The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p < 0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO 4 group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively. As shown in Table 4 , the heart coefficient in IDA group was significantly higher than that in Normal control group (P < 0.05). In addition, after supplementation of Fe-Gly, FeSO4 and Fe-F8, the heart coefficient showed some improvement, This result was also observed by Zhang et al[ 25 ]. In iron-deficient organisms, liver mass and volume are significantly reduced, which is typical of iron deficiency. The organ coefficients of the livers in iron enriched supplemented animals were lower than that of iron-deficient animals. Table 4 Organ index showed that spleen weight of mice in iron deficiency group was significantly higher than that in other groups (P < 0.05). This result was also observed by Yun et al[ 26 ].Kidney index showed no significant difference, and some studies showed that iron deficiency had no significant effect on spleen weight. 3.4. HE section of liver and kidney tissue The intestinal barrier plays an important role in the health of the body as it is the main immune, digestive and nutrient absorbing tissue. Colon tissue sections were obtained by H&E staining to further study the effect of iron supplementation on the intestinal tract of IDA mice.As shown in Fig. 2 , the mucosal epithelial cells of the colon tissue of mice in the normal control group were closely arranged and orderly, the lamina propria was intact, and the submucosal tissue was intact, with a large number of well-structured, long and clear crypts and villi, and the muscle fibers were neatly arranged and no lesions occurred.Compared with the normal control group, the colonic tissue of mice in IDA group had obvious changes in the overall structure, including mucosal epithelial cell arrangement defect, lamina propria atrophy, crypt structure damage, and epithelial barrier deformation. Wang et al. showed similar results in the colons of IDA mice[ 27 ].IDA reduces the antioxidant capacity of the body, leading to structural abnormalities and dysfunction of the organism biofilm, causing damage and inflammation of colon epithelial cells. After 4w of intragastric administration, part of the acines in the colon tissue of the FeSO4 group showed necrosis and defect and lamina propria atrophy, but the submucosal tissue was intact, the muscle fibers were arranged neatly and no lesions occurred.The colonic tissue structure of mice in Fe-Gly group and Fe-F8 group was orderly, the mucosa tended to be normal, the lamina propria was intact, the crypt structure basically returned to normal, inflammatory cell infiltration basically disappeared, the gap between the mucosa and the lower layer was significantly reduced, and the muscle fibers were neatly arranged without any lesions.These results indicate that supplementation of a certain dose of iron can effectively improve the intestinal mucosal barrier damage and inflammatory cell infiltration caused by IDA, among which Fe-Gly and Fe-F8 have better improvement effects than FeSO4 at the same dose. Wang's studies on other peptide iron chelates have also obtained similar results[ 28 ], In the FeSO4 group, intestinal tissue was damaged, a large number of mucosal epithelial cells were shed, villi erosion was observed, intestinal mucosa in the Marine fish skin peptide iron group, casein peptide iron group and whey protein group was improved, and the villi were intact and the glands were normal. Figure 3 The results of liver morphology of mice in each group showed that, compared with the normal control group, the hepatocyte cords around the central vein of the liver in IDA group were not clearly demarcated and arranged in a disorderly manner.After 4w of intragastric administration, the liver color of Fe-F8 group gradually turned red, and the lines of hepatocyte cords around the central vein were clear, and the arrangement tended to be neat. However, the cells in Fe-Gly group and FeSO4 group were improved to a certain extent, but the cord boundaries of hepatocytes were still unclear and the arrangement was chaotic.In conclusion, supplementation with a certain dose of Fe-F8 can significantly improve the damage of intestinal mucosal barrier and inflammatory cell infiltration caused by IDA, as well as the arrangement of liver cells, and the effect is better than Fe-Gly and FeSO4. 3.5. Hemoglobin level Hemoglobin levels before and after the trial are shown in Fig. 4 . Hemoglobin is a red blood cell protein responsible for transporting oxygen into tissues, where iron has a central place in the hemoglobin structure, IDA can cause hemoglobin to decline[ 29 ]. Due to iron deficiency diet, the hemoglobin content of the model group was the lowest, which was significantly lower than that of the normal control group (P < 0.05), Hb 83 ~ 84 g/L, belonging to the anemia level (less than 90g/L).After feeding iron supplement (Fe-Gly, FeSO4 and Fe-F8) for 4w, the hemoglobin level of the three iron supplement groups recovered, and the Hb content of the three iron supplement groups had no significant difference compared with the normal control group at the beginning of the experiment (P > 0.05).Among them, the hemoglobin content of Fe-F8 group can be restored to the original level of normal control group, and the results confirmed that yeast iron can promote the recovery of hemoglobin content. 3.6. Red blood cell count(RBC) and hematocrit level(HCT) Table 5 Hematological parameters of mice in different groups. Group Hb(g/L) RBC(*10 12 *L − 1 ) HCT(%) MCV(fL) Normal control group 139.93 ± 5.56 a 7.03 ± 0.61 bc 40.24 ± 2.13 b 70.12 ± 5.19 c IDA group 84.91 ± 3.89 e 6.43 ± 0.47 c 30.12 ± 1.94 e 64.42 ± 4.38 d Fe-Gly group 111.57 ± 3.81 c 7.91 ± 0.37 ab 36.27 ± 2.21 c 72.36 ± 6.01 b FeSO4 group 102.55 ± 4.13 d 7.01 ± 0.24 c 34.23 ± 2.72 d 69.19 ± 4.51 c Fe-F8 group 120.21 ± 6.74 b 8.04 ± 0.53 a 43.88 ± 3.10 a 75.27 ± 6.18 a * The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p < 0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO 4 group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively. Hemoglobin is a pigment protein present in red blood cells. The main physiological function of RBC is realized through hemoglobin, transporting oxygen to the body's tissue cells to produce energy for metabolism. Hematocrit (HCT) reflects the proportion of red blood cells in the whole blood[ 30 ].When iron intake is insufficient to meet daily needs, the body's iron stores drop too low to support normal red blood cell production, resulting in IDA, followed by low HCT, MCV, MCH, or MCHC[ 31 ]. The data in Table 5 show that after 4 weeks of recovery experiment, Hb of the anemia mice supplemented with iron-rich yeast recovers to 120.21 g/L, which is significantly different from that of the other two iron supplement groups, while the hemoglobin content of the Fe-Gly and FeSO4 groups is still lower than that of the normal control group.In addition, at the end of this experiment, RBC, HCT and MCV parameters of Fe-Gly group, FeSO4 group and Fe-F8 group were significantly higher than those of IDA group (P < 0.05). It is worth noting that RBC, HCT and MCV levels in Fe-F8 group were significantly higher than those in Fe-Gly group and FeSO4 group, indicating that Fe-F8 was more effective in improving anemia symptoms. 3.7. Serum iron level The changes of serum iron were shown in Fig. 5 . The serum iron concentration in IDA group was the lowest and the highest in Fe-F8 group, which was significantly different from Fe-Gly group and FeSO4 group (P < 0.05). In this study, the SI level in the IDA group was significantly lower than that in the normal control group (P < 0.05), which was similar to the result observed by C. Xiao et al[ 31 ]. The results showed that yeast iron could promote the recovery of serum iron content. 3.8. Serum iron level Table 6 The changes of serum immunoglobulin content in mice. Group IgG (g/L) IgM (g/L) Normal control group 17.59 ± 0.48 c 3.16 ± 0.12 d IDA group 11.76 ± 0.62 e 2.65 ± 0.08 e Fe-Gly group 22.55 ± 2.12 d 4.72 ± 0.19 c FeSO4 group 14.93 ± 0.31 b 5.01 ± 0.23 b Fe-F8 group 24.68 ± 2.37 a 6.06 ± 0.29 a * The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p < 0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO 4 group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively. Immunoglobulins are major players in humoral immunity. IgG is the most abundant immunoglobulin in the body, which is mainly responsible for neutralizing toxins and regulating immune cell function, while IgM is mainly distributed in the blood and is the first antibody to appear in the initial immune response[ 32 ]. After 4 weeks of iron supplementation, the contents of immunoglobulin IgG and IgM in the blood of mice were measured by anatomic blood collection, and the results were shown in Table 6 . The IgG and IgM contents of mice in IDA group were significantly lower than those in Normal control group (P < 0.05). The IgG and IgM contents in iron supplementation group were significantly different from those in Normal control group, indicating that supplementation with a certain dose of iron could improve the immune status of IDA mice, and it was noteworthy that Fe-F8 group was the highest (P < 0.05). 3.9. Antioxidant enzyme activity and MDA level and total antioxidant capacity The total antioxidant activity and MDA levels of mice in each group were shown in Fig. 6 and Table 6 . The results showed that compared with the model group, Fe-rich yeast could improve the total antioxidant capacity in serum of mice. The total antioxidant capacity of yeast group was 53.31%, which was higher than that of other experimental groups, but slightly lower than that of Normal control group. Table 7 The activities of antioxidant enzymes and the MDA level of mice in different groups. Group SOD (U/ml) GSH-PX (U/ml) MDA (umol/ml) Normal control group 151.90 ± 3.53 d 598.84 ± 24.49 c 24.98 ± 2.45 b IDA group 100.55 ± 2.34 e 464.77 ± 23.23 d 16.07 ± 1.91 c Fe-Gly group 187.90 ± 4.25 b 662.74 ± 28.13 b 17.55 ± 1.77 c FeSO4 group 157.80 ± 3.57 c 599.94 ± 29.08 c 16.87 ± 1.19 c Fe-F8 group 198.06 ± 8.61 a 669.90 ± 30.17 a 35.51 ± 3.07 a * The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p < 0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO 4 group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively. Compared with normal control group or iron supplement group, the activities of SOD and GSH-Px in IDA group were significantly decreased (P < 0.05), and the level of MDA in IDA group was also the lowest. In addition, the activities of SOD and GSH-Px in Fe-F8 group were higher than those in other iron supplement groups (P < 0.05), and the activities of SOD and GSH-Px in all groups were the highest. However, there was no significant difference in MDA concentration between FeSO4 group and IDA group. The experimental results show that the improvement effect of Fe-F8 group is more significant than that of Fe-Gly and FeSO4.Tang's research results show that compared with FeSO4, heme iron-enriching peptides can enhance the activity of antioxidant. 4. Discussion In this study, a strain F8 with iron-rich potential was selected. On this basis, the improvement effect of iron-rich yeast on iron-deficiency anemia was studied by using the iron deficiency anemia model of mice.Our results show that Fe-F8 can improve IDA in mice by weight, hematological parameters, organ parameters and antioxidant activity in vivo, We found that Fe-F8 has a more significant effect on IDA, with higher bioavailability and fewer side effects compared to Fe-Gly and FeSO4.This finding suggests that iron-rich yeast has great potential as an effective source of iron supplementation in IDA mice, and the effects of Strain no. F8 on other animals or humans need to be further studied. Declarations Funding: This research was funded by the National Key R&D Program of China (no.2021YFD1301000). Conflicts of Interest: All the authors declare no conflict of interest. Ethics approval：In accordance with the requirements of the National Act on the use of laboratory animals (People's Republic of China), animal use in current research concerns after evaluation and accreditation of the Association for the Care of Laboratory Animals (AAALAC) and Animal testing protocols are approved by the Animal Ethics Committee. Author Contributions: Conceptualization, Ying Chen; methodology, Hongbinig Wan; software, Yuanxiang Pang; validation and formal analysis, Hongbing Wan, Yuanxiang Pang; investigation, Xinyi Zhou; resources, Mingli Wan,Shengshuo Li; data curation, Ying Chen; writing—original draft preparation, Ying Chen; writing—review and editing, Ying Chen; visualization, Xuelian Liu; supervision, Xuelian Liu; project administration, Xuelian Liu. All authors have read and agreed to the published version of the manuscript. References Dallman P R. Biochemical Basis for the Manifestations of Iron Deficiency. Ann Rev Nutr, 2003, 6(1):13-40 . Hassan T H; Badr M A; Karam N A; et al. Impact of iron deficiency anemia on the function of the immune system in children. Medicine，2016，95(47): e5395 . Duque X; Martinez H; Vilchis-Gil J; et al. Effect of supplementation with ferrous sulfate or iron bis-glycinate chelate on ferritin concentration in Mexican schoolchildren: a randomized controlled trial. Nutrition Journal, 2014, 13(1):71 . Benoist B; McLean E; Egll I; et al. Worldwide prevalence of anaemia 1993-2005: WHO global database on anaemia. World Health Organization, 2008 . Lozoff B; Beard J; Connor J; et al.Long-lasting neural and behavioral effects of iron deficiency in infancy. Nutrition Reviews, 2006(5 Pt.2):64 . Georgieff M K. long-term brain and behavioral consequences of early iron deficiencyn. 48.[2023-11-22] . Wang F; Zhao W; Chen J; et al. Research progress of iron supplement. Advances in Pharmacy, 2016,40 (09): 680-688 . Dewey K G; Domello F M; Cohen R J; et al. Iron supplementation affects growth and morbidity of breast-fed infants: results of a randomized trial in Sweden and Honduras. Journal of Nutrition, 2002, 132(11):3249-3255. Hyder S M; Persson L A; Chowdhury A M; et al. Do side-effects reduce compliance to iron supplementation? A study of daily- and weekly-dose regimens in pregnancy. Journal of Health Population & Nutrition, 2002, 20(2):175-179 . A. I. Souza; M. B. Filho; C. C. Bresani; L. O. C; Ferreira and J. N. Figueiroa, Adherence and side effects of three ferrous sulfate treatment regimens on anemic pregnant women in clinical trials, Cad. Saude Publica , 2009, 25, 1225–1233 . Cancelo-Hidalgo; María Jesús; Castelo-Branco C; Palacios S; et al. Tolerability of different oral iron supplements: a systematic review. Current Medical Research & Opinion, 2013, 29(4):291-303 . Bovellbenjamin A C; Viteri F E; Allen L H. Iron absorption from ferrous bisglycinate and ferric trisglycinate in whole maize is regulated by iron status. American Journal of Clinical Nutrition, 2000, 71(6):1563 . Chaud M V; Izumi C; Nahaal Z; et al. Iron derivatives from casein hydrolysates as a potential source in the treatment of iron deficiency. Journal of Agricultural & Food Chemistry, 2002, 50(4):871 . Miquel E; Rosaura Farré. Effects and future trends of casein phosphopeptides on zinc bioavailability. Trends in Food Science & Technology, 2007, 18(3):139-143 . Kyyaly M A; Powell C; Ramadan E. Preparation of iron-enriched baker's yeast and its efficiency in recovery of rats from dietary iron deficiency. Journal of Nutrition,2015,31(9): 1155-1164 . Geng Q. Culture of iron-rich yeast cells and its application in the treatment of iron-deficiency anemia. Ocean University of China, China, 2014. Zhang X G; Peng Y N; Li X R; et al. Screening of iron-enriched fungus from natural environment and evaluation of organically bound iron bioavailability in rats. Food Science and Technology, 2015, 35(1):58-65 . Zhang X; Hu Z; Luo Y; et al. Pharmacokinetics and bioavailability of iron-rich yeast. Chinese Journal of Veterinary Medicine, China, 2004, 23(2):4. Yuan Y; Guo X; He X; et al. Construction of a high-biomass, iron-enriched yeast strain and study on distribution of iron in the cells of Saccharomyces cerevisiae. Biotechnology Letters, 2004, 26(4):311-315. Xue D; Zhang H; Zhao X. Fermentation culture and nutritional evaluation of ferric nutritive yeast . Food Science, 2003, 24(9):4. Paš M; Piškur B; Šuštarič M; et al. Iron enriched yeast biomass–A promising mineral feed supplement. Bioresource technology, 2007, 98(8): 1622-1628 . Zhang X G; Wang N; Ma G D; et al. Preparation of S-iron-enriched yeast using siderophores and its effect on iron deficiency anemia in rats. Food Chemistry, 2021, 365(2):130508 . Zheng Y; Li X; Xie Y; et al. Effect of iron deficiency anemia on the reproduction of female rats and the development of their offspring. Wei Sheng Yan Jiu, 2001, 30(3):163-165 . Fu-Rong; Wang; Zhong-Guo; et al. Effectiveness of treatment of iron deficiency anemia in rats with squid ink melanin-Fe. Food & function, 2014 . Zhang X G; Wei G X; Wang W N; Ma G D; Tang P; Chen X Q. Effects of Fe-YM1504 on iron deficiency anemia in rats. Food Funct. 2016 Jul 13;7(7):3184-92 . Yun S; Zhang T; Li M; et al. Proanthocyanidins Inhibit Iron Absorption from Soybean (Glycine max) Seed Ferritin in Rats with Iron Deficiency Anemia. Plant Foods for Human Nutrition, 2011, 66(3):212-217 . Wang M; Wan P; Zhu L; et al. Colon injury induced by iron deficiency anemia (IDA) in mice. Natural Science Journal of Heilongjiang University, China, 2019, 36(2):7. Wang M. Effect of dietary oligopeptide iron on iron deficiency anemia in rats [D]. Zhejiang University, China, 2013. Linberg R; Conover C D; Shum K L; et al. Hemoglobin based oxygen carriers: how much methemoglobin is too much? Taylor & Francis, 1998(2) . Chen C; Zhou Y; Dong Y. Diagnostic value of MCV,RDW,MCH and MCHC combined with trace elements iron, zinc and copper in children with iron deficiency anemia. Experimental and Laboratory Medicine, 2021 . Xiao C; Lei X; Wang Q; et al. Effects of a Tripeptide Iron on Iron-Deficiency Anemia in Rats. Biological trace element research, 2015, 169(2):211-217. Wang X; Sun L. Effect of iron supplementation on immune function in patients with iron deficiency anemia. Shandong Medicine, 2014, 000(020):68-69. Tang N; Chen L Q; Zhuang H. Effects of heme iron enriched peptide on iron deficiency anemia in rats. Food & Function, 2014, 5(2):390-399. Additional Declarations No competing interests reported. Cite Share Download PDF Status: Published Journal Publication published 28 Mar, 2024 Read the published version in BioMetals → Version 1 posted Editorial decision: Revision requested 19 Jan, 2024 Reviews received at journal 14 Jan, 2024 Reviewers agreed at journal 31 Dec, 2023 Reviewers agreed at journal 31 Dec, 2023 Reviewers invited by journal 30 Dec, 2023 Editor assigned by journal 27 Dec, 2023 Submission checks completed at journal 27 Dec, 2023 First submitted to journal 17 Dec, 2023 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3769317","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":264539902,"identity":"5d0e2c9b-6e30-4f4f-b2db-2145282eae24","order_by":0,"name":"Ying Chen","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA2UlEQVRIie3PIQvCQBTA8ScHz3J49YZDv8LEYhD8KncIa4JxYWGguODEr2I0PhGWTqzGiWDWIosOtCnbbIb75/fjvQdgs/1h2D3eSOUhRwY6U0FYTVoSenROUlc04eJlJq0mHQn9XYZs6KwbV+c8ZzUOa0eKFEfu7Zkf6AhBxEtVTlwiUgO3IOif9NYFaQ6bcgI6em/hBTEInpxUkTGQQlYQ8ZjqBatBpP8izgx8qEe4AdJJygWDsVQm5ZW/dOOE3fM8HKEgfc+DsCPiVTn5iP82brPZbLavPQGu9Uhw915aIgAAAABJRU5ErkJggg==","orcid":"","institution":"","correspondingAuthor":true,"prefix":"","firstName":"Ying","middleName":"","lastName":"Chen","suffix":""},{"id":264539903,"identity":"0b6e3882-e229-4b8b-b07d-5cdd488af3b8","order_by":1,"name":"Yuanxiang Pang","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Yuanxiang","middleName":"","lastName":"Pang","suffix":""},{"id":264539904,"identity":"01248f20-ab23-4553-a233-52097f704abc","order_by":2,"name":"Hongbing Wan","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Hongbing","middleName":"","lastName":"Wan","suffix":""},{"id":264539905,"identity":"840abda5-cf87-4ddd-80ea-fd396c908f58","order_by":3,"name":"Xinyi Zhou","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Xinyi","middleName":"","lastName":"Zhou","suffix":""},{"id":264539906,"identity":"9a413b56-24ca-4a87-a9cd-70b8cc947e06","order_by":4,"name":"Mingli Wan","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Mingli","middleName":"","lastName":"Wan","suffix":""},{"id":264539907,"identity":"07fc8b31-19f7-40ad-8a20-174fd215b1a7","order_by":5,"name":"Shengshuo Li","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Shengshuo","middleName":"","lastName":"Li","suffix":""},{"id":264539908,"identity":"9923bf10-4ead-407a-935e-ecbd17990766","order_by":6,"name":"Xuelian Liu","email":"","orcid":"","institution":"","correspondingAuthor":false,"prefix":"","firstName":"Xuelian","middleName":"","lastName":"Liu","suffix":""}],"badges":[],"createdAt":"2023-12-18 00:59:14","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-3769317/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3769317/v1","draftVersion":[],"editorialEvents":[{"content":"https://doi.org/10.1007/s10534-024-00592-3","type":"published","date":"2024-03-28T15:01:47+00:00"}],"editorialNote":"","failedWorkflow":false,"files":[{"id":49020607,"identity":"da444e47-9011-4bfc-979d-22c3462b0532","added_by":"auto","created_at":"2024-01-01 08:50:55","extension":"png","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":9594,"visible":true,"origin":"","legend":"\u003cp\u003eChanges of body weight in different groups of mice. The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO4 group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e","description":"","filename":"1.png","url":"https://assets-eu.researchsquare.com/files/rs-3769317/v1/15e81922cc74179be429ac2f.png"},{"id":49020468,"identity":"3d05b7bc-bc26-4fd9-8a63-ff7f4809fa5a","added_by":"auto","created_at":"2024-01-01 08:42:55","extension":"png","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":230101,"visible":true,"origin":"","legend":"\u003cp\u003eColon tissue morphology of mice in each group（H\u0026amp;E staining, 200x magnification).\u003c/p\u003e","description":"","filename":"2.png","url":"https://assets-eu.researchsquare.com/files/rs-3769317/v1/7c6710433a679a3c5012f408.png"},{"id":49020471,"identity":"e608fea2-2aca-4a32-b08f-5940de369fc8","added_by":"auto","created_at":"2024-01-01 08:42:55","extension":"png","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":257582,"visible":true,"origin":"","legend":"\u003cp\u003eLiver morphology of mice in each group（H\u0026amp;E staining, 200x magnification).\u003c/p\u003e","description":"","filename":"3.png","url":"https://assets-eu.researchsquare.com/files/rs-3769317/v1/33d5c39df3278efb6ae126f4.png"},{"id":49020466,"identity":"84286d6f-0a46-4acd-8b54-9209c69a627d","added_by":"auto","created_at":"2024-01-01 08:42:55","extension":"png","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":9722,"visible":true,"origin":"","legend":"\u003cp\u003eThe hemoglobin changes of mice in different groups before and after the recovery experiment.The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO\u003csub\u003e4\u003c/sub\u003e group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e","description":"","filename":"4.png","url":"https://assets-eu.researchsquare.com/files/rs-3769317/v1/fa9a13fe5801df142c8e29fe.png"},{"id":49020690,"identity":"714468ff-0bfc-4f44-b18a-23eb6b394483","added_by":"auto","created_at":"2024-01-01 08:58:55","extension":"png","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":9746,"visible":true,"origin":"","legend":"\u003cp\u003eSerum iron levels in different groups.Data are presented as mean ± SD . Different letters show statistically significant differences (p \u0026lt; 0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO\u003csub\u003e4\u003c/sub\u003e group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e","description":"","filename":"5.png","url":"https://assets-eu.researchsquare.com/files/rs-3769317/v1/8f46b730452231a45ed4777f.png"},{"id":49020469,"identity":"4c861264-3672-4079-9d70-295085f5975c","added_by":"auto","created_at":"2024-01-01 08:42:55","extension":"png","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":9201,"visible":true,"origin":"","legend":"\u003cp\u003eEffects of different treatments on total antioxidant capacity of mice.IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO4 group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e","description":"","filename":"6.png","url":"https://assets-eu.researchsquare.com/files/rs-3769317/v1/96af5505934e7528b50c937d.png"},{"id":53869783,"identity":"d2898424-2159-4bd9-b5fb-db96cd5c60a9","added_by":"auto","created_at":"2024-04-01 15:11:37","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1033192,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3769317/v1/edef4054-05c2-4a38-bf72-91a503fecb7e.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Production of Iron-enriched Yeast and it’s application in the treatment of iron-deficiency anemia","fulltext":[{"header":"1. Introduction","content":"\u003cp\u003eIron is an essential trace element for most life forms and is involved in many biological processes\u003csup\u003e[1]\u003c/sup\u003e. Iron deficiency is one of the most common nutritional deficiencies worldwide. Severe iron deficiency can cause iron deficiency anemia (IDA), and iron deficiency can impair immune response\u003csup\u003e[2]\u003c/sup\u003e, physical work ability and intellectual function\u003csup\u003e[3]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eIron ion is the second most abundant metal element in the Earth's crust, but iron deficiency, especially iron deficiency anemia, is a common nutrient deficiency in humans, affecting about 1.6\u0026nbsp;billion people worldwide\u003csup\u003e[4]\u003c/sup\u003e. Iron plays a crucial role in the normal development of the central nervous system, which can affect myelin formation, neuronal and glial energy metabolism, and neurotransmitter production \u003csup\u003e[5]\u003c/sup\u003e. Iron deficiency during the critical period of development will have irreversible harmful effects on the brain. Lead to cognitive and behavioral abnormalities\u003csup\u003e[6]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eTo improve iron deficiency anemia, several forms of iron supplements have been developed over the past two decades. At present, the research on iron supplements is more concerned about the blood-replenishing activity of iron supplements, and the side effects of iron supplements such as intestinal inflammation are still lacking.\u003c/p\u003e \u003cp\u003eAt present, oral iron supplements used in clinical practice are mostly traditional iron supplements, such as FeSO\u003csub\u003e4\u003c/sub\u003e, ferrous succinate, ferrous fumarate, ferrous gluconate, ferrous glycine, etc., but they all have iron odor, instability, low bioavailability, and are prone to dietary taboo, free iron toxicity, gastrointestinal discomfort and other symptoms\u003csup\u003e[7]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eFeSO\u003csub\u003e4\u003c/sub\u003e was first used in clinical treatment, easily soluble in water, good bioavailability, low cost, is the most widely used oral iron agent. However, its bioavailability is low, accompanied by side effects such as stomach pain and diarrhea, and it is easy to cause lipid peroxidation and even DNA damage \u003csup\u003e[8\u0026ndash;9]\u003c/sup\u003e FeSO\u003csub\u003e4\u003c/sub\u003e has high iron content and good absorption effect, which may help alleviate the symptoms of iron deficiency and anemia to a certain extent. But it can cause many adverse reactions, such as diarrhea and constipation\u003csup\u003e[10]\u003c/sup\u003e. Other common inorganic iron agents include FeCl\u003csub\u003e3\u003c/sub\u003e\u0026middot;6H\u003csub\u003e2\u003c/sub\u003eO, FeCO\u003csub\u003e3\u003c/sub\u003e, FeNaPO\u003csub\u003e4\u003c/sub\u003e and Fe\u003csub\u003e2\u003c/sub\u003eP\u003csub\u003e2\u003c/sub\u003eO\u003csub\u003e7\u003c/sub\u003e.\u003c/p\u003e \u003cp\u003eOrganic ferric agents are mainly some small molecular organic acid ferric salts, such as ferrous fumarate (43.4%), ferric albuminate (7%) and Fe-Gly \u003csup\u003e[11]\u003c/sup\u003e. Studies have shown that Fe-Gly iron is better absorbed than FeSO4 iron\u003csup\u003e[12]\u003c/sup\u003e.However, recently, many new iron supplements have appeared, such as the chelation of iron with peptides derived from proteins in hydrolyzed foods, and the conversion of iron into organic iron by cells\u003csup\u003e[13\u0026ndash;14]\u003c/sup\u003e. There are also some new macromolecular complexes such as polypeptide iron chelates, heme iron, polysaccharide iron, iron-rich yeast, etc. These have caused great concern.\u003c/p\u003e \u003cp\u003eAmong them, iron-rich yeast has the characteristics of high absorption rate, low irritation and rich nutrition. Inorganic iron can be converted into organic form through the yeast reproduction process, which makes iron more easily absorbed by the body. It is a good new iron supplement for preventing and treating IDA.Studies have shown that iron-rich bread yeast can improve the levels of red blood cells (RBC) and HGB in anemic mice, and can alleviate the damage caused by anemia to various organs of the body\u003csup\u003e[15]\u003c/sup\u003e. The form of iron in iron-rich yeast is similar to that in human body, so iron-rich yeast does not have iron toxicity caused by free iron ion enrichment. Studies have shown that compared with FeSO4 and amino acid chelated iron, iron-rich yeast has a higher bioavailability, showing a good effect on IDA treatment\u003csup\u003e[16]\u003c/sup\u003e.\u003c/p\u003e \u003cp\u003eIn conclusion, the treatment of IDA should increase iron intake and iron absorption and availability, reduce intestinal adverse reactions as much as possible, and thus improve the effectiveness of treatment and patient compliance. Therefore, the aim of this study was to investigate the improvement of yeast iron (Fe-F8) on iron deficiency anemia mice. The effects of yeast iron(Fe-F8) on hematological parameters and organs of mice were studied. In addition, the antioxidant effects of Fe-F8 on anemic mice were evaluated to investigate whether Fe-F8 is promising for development as a new iron supplement.\u003c/p\u003e"},{"header":"2. Materials and Methods","content":"\u003cp\u003e\u003cstrong\u003ePreparation of iron-rich yeast, F8 and cell iron content evaluation\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e(1) Test tube culture seed solution\u003c/p\u003e\n\u003cp\u003eStrain Fe-F8 was immersed in a test tube containing 10 mL YPD medium (2% glucose, 1% yeast powder, 2% tryptone) at 30℃ and 200 rpm for 18 h. Then, it was inoculated in 250 mL flask of 50 mL YPD medium at a ratio of 2% (v/v) at 30℃, 200 rpm, and oscillated for 18 h as seed liquid for reserve.\u003c/p\u003e\n\u003cp\u003e(2) Flask culture\u003c/p\u003e\n\u003cp\u003eThe strain Fe-F8 seed liquid was inoculated in 250 mL shaking bottle with 50 mL YPD medium at 30℃ and 200 rpm at a ratio of 2% (v/v), and oscillated for 18 h as secondary seed liquid.\u003c/p\u003e\n\u003cp\u003e(3) Fermentation culture\u003c/p\u003e\n\u003cp\u003eThe secondary seed solution was added to the fermentation medium (molasses 2%, (NH4) 2SO4 0.6%, KH2PO4 1.0%, pH 5.1) at 10% (v/v). Add iron salt before inoculation, take 36 mL of 1.5L fermentation solution and add it into culture solution. The fermentation time was 28 h. Fermentation temperature 30℃, rotational speed: 0\u0026ndash;2 h: 200 rpm; 2\u0026ndash;5 h: 300 rpm; 5\u0026ndash;8 h: 400 rpm; 8\u0026ndash;10 h: 500 rpm; 10\u0026ndash;12 h: 600 rpm; After 12 hours: 700 rpm; If the dissolved oxygen is less than 30%, the speed is adjusted to 800 rpm. Maintain fermentation pH at 4.4 with 20% NaOH solution. The dissolved oxygen is controlled at 50%-60%.\u003c/p\u003e\n\u003cp\u003eAfter fermentation was completed, the ferrified yeast cells with a dry weight of about 0.1g were weighed into a digestion bottle, and 5 mL of digestion liquid (HNO3:HClO4\u0026thinsp;=\u0026thinsp;5:1) was added to the heating plate at 200℃ for digestion until the liquid was clear and transparent. Then, the cells were removed and cooled, and the volume of distilled water was fixed to 25 mL, and 1 mL of constant volume liquid was absorbed and 4 mL of distilled water was added. After mixing well, absorb 200 \u0026micro;L into 7 ml EP tube, add 3.0 mL distilled water, add 0.1 mL 1M hydrochloric acid solution, 0.1 mL 10% hydroxylamine hydrochloride, 0.1 mL 0.12% o-diazophene (1, 10-phenanthroline), add 0.5 mL 10% sodium acetate, shake well. The content of cell iron in ferriferous yeast was calculated at 510 nm by using the absorbance value of the blank solution without ferriferous agent as reference solution.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnimals and experimental design\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAnimals\u003c/strong\u003e\u003c/p\u003e\n\u003cdiv class=\"BlockQuote\"\u003e\n \u003cp\u003eThe 3-week-old healthy male KM mice were from Beijing Weitonglihua Laboratory Animal Technology Co., LTD. According to the requirements of the National Act on the use of laboratory animals (People\u0026apos;s Republic of China), all animals are used in the current research after the evaluation and accreditation of the Association for the Care of Laboratory Animals (AAALAC) and the protocol for Animal testing is approved by the Animal Ethics Committee. All mice were housed in stainless steel cages with sawdust pads and kept in an environmentally controlled room (24\u0026thinsp;\u0026plusmn;\u0026thinsp;2\u0026deg;C and 50% \u0026plusmn; 10% relative humidity) for a 12-hour light/dark cycle. The sawdust mat is renewed every 3 days and the rats have free access to food and water.\u003c/p\u003e\n\u003c/div\u003e\n\u003cp\u003e\u003cstrong\u003eExperimental design\u003c/strong\u003e\u003c/p\u003e\n\u003cdiv class=\"BlockQuote\"\u003e\n \u003cp\u003eAfter 3 days of adaptation, 60 3-week-old male KM mice were randomly divided into normal control group (n\u0026thinsp;=\u0026thinsp;12) and model group (n\u0026thinsp;=\u0026thinsp;48). The normal control group was fed the standard diet of AIN-93G throughout the experiment. The model group was fed AIN-93G diet, containing 8 mg iron per kg diet, TROPHIC Animal Feed. High-tech Co., Ltd,Nantong, China) induced IDA model. The whole experiment process was strictly controlled, iron staining was avoided, and hemoglobin (Hb) levels were analyzed weekly. After 4 weeks, IDA with Hb content less than 90 g/L was taken as IDA, and then recovery experiment was carried out.\u003c/p\u003e\n \u003cp\u003eAfter IDA mouse model was established, IDA mice (n\u0026thinsp;=\u0026thinsp;48) were randomly divided into 4 groups with 12 mice in each group. Hb concentration was balanced. IDA iron deficiency model group was given the above iron deficiency diet. Organic iron group was given 3.0mg Fe/kg BWFe-Gly; The inorganic iron group was given 3.0mg Fe/kg BW FeSO4, and the sample group was given 3.0mg Fe/kg BW yeast iron Fe-F8. Fe-Gly, FeSO4 and Fe-F8 were dissolved in distilled water, and the mice were given the above dosage by intragastric administration. The normal control group and IDA model group were given the same volume of deionized water. All supplements were prepared fresh before use and administered by gavage at 10 am daily for 4 weeks. During this time, weigh yourself once a week.\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003eSample collection\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003eAt the end of the whole experiment, the weight of the mice was measured after fasting for 12 hours, the mice were dissected, and blood was collected and placed in the heparin sodium anticoagulant tube. The collected samples were centrifuged to obtain plasma and serum and frozen at \u0026minus;\u0026thinsp;20\u0026deg;C for further analysis. All mice, heart, liver, stomach, kidney, spleen, and colon, were rinsed with normal saline, weighed, and stored at \u0026minus;\u0026thinsp;80\u0026deg;C for further analysis.\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003eHematological test\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003eThe hemoglobin content (Hb), red blood cell count (RBC) and hematocrit (HCT) in the blood were measured by the kit. HCT is expressed as the percentage of red blood cells in total blood volume. Serum iron (SI) concentration was measured using the SI test kit (Beijing Solebol Technology Co., LTD., Beijing, China) according to the manufacturer\u0026apos;s instructions.\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003eOrgan coefficient\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003ePathological examination of mice was performed by naked eye during dissection. All mice had their hearts, livers, stomachs, kidneys, spleens and colons removed and weighed. The relative weight of each organ is calculated based on the final weight measured that day. The organ coefficient is calculated as follows:\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv class=\"gridtable\"\u003e\n \u003cdiv align=\"left\" class=\"colspec\"\u003e\u003cbr\u003e\u003c/div\u003e\u0026nbsp;\u003cimg src=\"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAYMAAAAdCAYAAABIfwCVAAAAAXNSR0IArs4c6QAAAARnQU1BAACxjwv8YQUAAAAJcEhZcwAADsMAAA7DAcdvqGQAAABfaVRYdFNuaXBNZXRhZGF0YQAAAAAAeyJjbGlwUG9pbnRzIjpbeyJ4IjowLCJ5IjowfSx7IngiOjM4NywieSI6MH0seyJ4IjozODcsInkiOjI5fSx7IngiOjAsInkiOjI5fV19JzJbNgAACKFJREFUeF7tnb3OTU0Ux/fzXoaICK5BISgUuAAFKpWEWmiUGqImUalQuAAUCkThGhARcRve/Rv7f/wtsz/OOfvk+Tjrl0z2nlnzsWat+dgz5xE7v1qaJEmSZKv5r3smSZIkW0xuBkmSJMlmN4MXL140Ozs7f4Vt5OLFi4v+YxO4efPmIu3BgweL5xRk169fv3YpyW6CL0+cONHFxiE/Y2IVKLsp6MPUMTgF+rhqP+dmWV3Iu4qtP3z4UOZmH5q75KuBjNCn65h8LfjNYBNcuHCB3yK62G/u379f0p4/f96lHHxu3LhR+q3348ePlzjvIJsk2wF+x9/Mj2X58uXLYizNDeMSveaqX/N/lX7uNtJdc3QKPo+x5fv370ucp1C9MV14OvVF243J12Ujq5AGPIM3IqPVZAeRmuMZLHtpQ9yPE3Y/w/wYs3lNztxZZ96MtakPlbmgvf06ttB7aDPAD1GutY2yPGtznLWgtibUFnfPNyafg9mvibi6ePz4cdMaqjl27FiX+odLly6V58OHD8vzINN3jdMOpO5t9+HY+vr16y6W7AX6rie+fftWnVNTSD/PS81H+AdWsfPbt2+bc+fOdbHftIt/8/Hjx/I+Jp+D2TeDnz9/lufRo0fLM8Jgbr9Amjdv3pS47th0b667V92txaC7NvJ5ui+8xCmPw2ryCDLlU1nHZXEQxLLSj/7QTzhz5kyRqY9w5cqVRX7V4e0O6eT1CO+r3yfKjhDl1IMeQHptgKtMX6COPtxH8Y5T99OSux1iUBveR4LbhPqRq79R7rhetOn1xrZkE6+X4NB2rX+enxDzaOwT1C55+JhiQSFdfSDv2bNnyzuov2qHfF4fQe1R95ifHcqpDuzjDPkUJHdZ9Ct6QrSx4/nBdZJNlCbb8VQe9HCIx3aU10PM435Xu9SFf/AT6fSHtonzoce8b7/Yi83V1zGo78iRI13sD77BDMlnoTshzAZHI6odOm5yHCXoyETwI5fS/YhEXEdk4pQXvKu86iPomObyCHXW2hIuU17VRdz1UN+lZ6xbkCbdlKeWVtNJ74oD+qgsqL/Sx/NTJ++qW3nmBh1cJ9rQMReZ6ySI0z+QnqpDcdmW/sn21Kv65BuX10CuvBDtQDvSBZnn9brVtvoGvHvc9QLevQztuK7IvTyQpr4jozxBaUAd0X7L+JnyXkbtCOR9PoVa+31yQHe3S4Synj/aiTakDzLPS1m1rX55W6Qpjh7IY3kvw9P7wntNd/W7D8llY0Ga2xa8jTH5HAyPjhVQZ92wERwhpyq/D+o4cOWsaEAgneBGIe6GGzIa6X0y+uADAKQbOiFX+x7Udp/engeUT2lDOoHaFbzHIPtKXxF1ivI5oE61L+RntYvcx0jNVjGPwCfk9TaijykXdXBivxXnqTg6gdqLweU+TqLe6OXyGI+6RDnE8YA8pgnVR1jGz30+oSwh2tN9WpOj41A/aUs2rBF9SNz75LqSD1kMArnbCxn6iGjPGI+6DNl/CLeZE/UBb2NMPgezXxMdOnSoPLnjqsGRsR0Azfnz57uUf7l8+XJ56lj26dOn8jx9+nR5go5ubR/K3dmqxGOwUzuCHT58uDy5DkPeDpKigwfpvypDOkWUN+rw+fPnkr4usnNf0BHd+f79e/f2B42LHz9+lGeE60P8+OzZsxLXODl16lSJg64F7t69W+y+DnGMoXM7sZqnT5+W+Lt37xb389iynbz/2Ljv/v769evNkydPulhTrkTjfe8yxCuiPnSlge7Ybl28f2M+rckj2LxdUBdjhvnTZ0Pg90X64VctPkZ8fpKPEH3Uh/sauIaZYuNNQb+iDRl3um4fk8/B7JsBzsXQGLe2qL18+bI8b926VZ59tLvg4l6dJ5NRsCjQxpCzp4K+Q4sv/ajBRMARfZveOozp5GgyTb2bXJY4uWK4fft2l/NvmJi1PmgzrfHo0aPFPSyLBgu+PgBYQBj8tOkfBeugBQHbcR979erVxbj1ScYd8TI/1Mkm9INA+T47TYHFb+wDA501T7DjHMh/J0+eLM8xnyIf4969e82dO3cmbXCMbS3+bHR8GFy7dq2MEfTw8owXfTROARvha/mIsbDuR9w68LEQ1xLsqT+4GZPPwUb+0RmGxjkEhwnNQGCh1yJWA0czSX3RiQuAD8q+BXsKWgD8C5eBx2DVhuU/hjEwGTjojyNiWcrpa3NVhnSqgT78SO2M/UhYw9tbBy18fvrjI4CJPbSQk999HhdQX2zm2IRlZxZ6FgJ0Y8yih59IWIC0gAlsVVsYAdv7+H316lUnWZ5lfaKT19DCOFSn25V+aKyP+VSLksYdtsG2BJ8/Oh0wXqcsvtiekxVfxbSjMuihTQo4jbEZuk+G5gA6MZ7ko1U3UNpbd74D9sVWGmNuexiTz0JriI3BPRtNeHC4A3OZ7tF4erpCO4iq8tYoC7mn655P8XbglvKRWn2iHTC9Mohl1UbsO3rE/hJi/boX7NMp1ito19MpH/PG9ol7+5SZE2/Lbe/pbk9P94COUS6fE6KPXUYYAjl2EhozkWhLlXG7q4/RFwrY22W0E+ulr+4n3qNf+sa099vfl/Gz1+12EZIRvG3wMUs9yGMeQJ9a3TWkN2UEfavVG/2uMt4n8sQ0D/Qh2jfWC/IbeacS65Euwn1U69+YfF02uhmsiiZEBEcNDeRkf9O3QExdOPYKB6UfmwI7MMd3k/TRv2zkmmhd/CjqcN84dM2Q7F849tZ+sCd9znvRTcOVgf94LEiv/Z34NsJV1KzXG0vCNVntmpF0vx7cOrpNYU/hxyEPu/01kWyW2tG9dkLc68TrAEK8Etg2uNaQLfYCro/Ctp/c8j+3SZIkSfL/M0iSJElyM0iSJElacjNIkiRJcjNIkiRJcjNIkiRJWnIzSJIkSXIzSJIkSXIzSJIkSVpyM0iSJElyM0iSJElyM0iSJEmapvkfgQqqmM2yR58AAAAASUVORK5CYII=\"\u003e\n\u003c/div\u003e\n\u003cp\u003e\u003cstrong\u003eDetermination of antioxidant enzyme activity and malondialdehyde level and total antioxidant capacity\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eSerum glutathione peroxidase (GSH-PX) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content and total antioxidant capacity (T-AOC) were measured using a diagnostic kit (Beijing Solaibo Technology Co., LTD., Beijing, China) according to the manufacturer\u0026apos;s instructions.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eStatistical analysis\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eData are expressed as mean\u0026thinsp;\u0026plusmn;\u0026thinsp;SD and analyzed by one-way analysis of variance (ANOVA) and Duncan\u0026rsquo;s multiple range tests using SPSS 17.0 software. P\u0026thinsp;\u0026lt;\u0026thinsp;0.05 was considered statistically significant.\u003c/p\u003e"},{"header":"3. Results","content":"\u003cdiv id=\"Sec4\" class=\"Section2\"\u003e \u003ch2\u003e3.1. Preparation and determination of iron content in yeast\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab1\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 1\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eScreening results of iron-rich yeast.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003eStrain\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"2\" nameend=\"c3\" namest=\"c2\"\u003e \u003cp\u003eCDM medium\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"2\" nameend=\"c5\" namest=\"c4\"\u003e \u003cp\u003eMolasses culture medium\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003e\u003cb\u003eBiomass (g/L)\u003c/b\u003e\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003e\u003cb\u003eCell iron content (mg/g)\u003c/b\u003e\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003e\u003cb\u003eBiomass (g/L)\u003c/b\u003e\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003e\u003cb\u003eCell iron content (mg/g)\u003c/b\u003e\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e8.6\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.05\u0026thinsp;\u0026plusmn;\u0026thinsp;0.03\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e9.1\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e0.99\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e9.0\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.21\u0026thinsp;\u0026plusmn;\u0026thinsp;0.04\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e10.1\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e1.06\u0026thinsp;\u0026plusmn;\u0026thinsp;0.04\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e8.4\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.46\u0026thinsp;\u0026plusmn;\u0026thinsp;0.03\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e9.7\u0026thinsp;\u0026plusmn;\u0026thinsp;0.4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e1.31\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e8.7\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.10\u0026thinsp;\u0026plusmn;\u0026thinsp;0.03\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e9.9\u0026thinsp;\u0026plusmn;\u0026thinsp;0.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e1.03\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e7.8\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.35\u0026thinsp;\u0026plusmn;\u0026thinsp;0.03\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e9.6\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e1.30\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e9.6\u0026thinsp;\u0026plusmn;\u0026thinsp;0.2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.15\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e11.8\u0026thinsp;\u0026plusmn;\u0026thinsp;0.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e0.99\u0026thinsp;\u0026plusmn;\u0026thinsp;0.03\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF7\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e6.8\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.32\u0026thinsp;\u0026plusmn;\u0026thinsp;0.04\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e8.5\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e1.27\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e11.2\u0026thinsp;\u0026plusmn;\u0026thinsp;0.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.62\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e13.0\u0026thinsp;\u0026plusmn;\u0026thinsp;0.3\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e1.49\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003eF9\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e7.1\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e1.16\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e8.9\u0026thinsp;\u0026plusmn;\u0026thinsp;0.1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e1.09\u0026thinsp;\u0026plusmn;\u0026thinsp;0.04\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003e \u003csup\u003e*\u003c/sup\u003e Strain no. F1-F9 was obtained from lab-preserved strains. Molasses medium: molasses 2%, (NH\u003csub\u003e4\u003c/sub\u003e)\u003csub\u003e2\u003c/sub\u003eSO\u003csub\u003e4\u003c/sub\u003e 0.6%, KH\u003csub\u003e2\u003c/sub\u003ePO\u003csub\u003e4\u003c/sub\u003e 1.0%, FeSO\u003csub\u003e4\u003c/sub\u003e\u0026middot;7H\u003csub\u003e2\u003c/sub\u003eO 5mM, pH 5.1; CDM medium: Pancreatic digest of casein 1.7%, Papaic digest of soybean meal 0.3%, Sodium choride 0.5%, Dipotassium hydrogen phosphate 0.25%, C\u003csub\u003e6\u003c/sub\u003eH\u003csub\u003e12\u003c/sub\u003eO\u003csub\u003e6\u003c/sub\u003e\u0026middot;H\u003csub\u003e2\u003c/sub\u003eO 0.25%, pH 7.3.\u003c/p\u003e \u003cp\u003eStrain no. F8 was selected according to its biomass and enrichment rate of iron ions. Studies have shown that iron ion can inhibit the growth of microorganisms[\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e], so in order to obtain strains with high iron and high biomass, it is necessary to select the best concentration of iron ion in the medium.\u003c/p\u003e \u003cp\u003eAs shown in Table\u0026nbsp;\u003cspan refid=\"Tab1\" class=\"InternalRef\"\u003e1\u003c/span\u003e, Strain no. F8 has its unique growth characteristics, with high biomass and cell iron content in molasses medium. Although the iron-rich strains in CDM medium had higher cell iron content, their biomass was low, and CDM medium was not suitable for large-scale fermentation due to its complex composition, cumbersome configuration and high cost, so molasses medium was used for further optimization. Furthermore, the effects of adding FeSO4\u0026middot;7H2O or triferric citrate in molasses medium with different concentration gradients on cell growth and iron enrichment of Fe-rich strain F8 were investigated.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab2\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 2\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eIron enrichment results of iron-rich yeast F8 in molasses medium with different iron concentrations.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"char\" char=\"\u0026plusmn;\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003eIron concentration(mM)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"2\" nameend=\"c3\" namest=\"c2\"\u003e \u003cp\u003eBiomass (g/L)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"2\" nameend=\"c5\" namest=\"c4\"\u003e \u003cp\u003eCell iron content (mg/g)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eFeSO4\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eFerric citrate\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eFeSO4\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eFerric citrate\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e2\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e9.53\u0026thinsp;\u0026plusmn;\u0026thinsp;0.46\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e10.39\u0026thinsp;\u0026plusmn;\u0026thinsp;0.19\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e1.83\u0026thinsp;\u0026plusmn;\u0026thinsp;1.75\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e0.78\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e4\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e10.56\u0026thinsp;\u0026plusmn;\u0026thinsp;0.17\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e10.99\u0026thinsp;\u0026plusmn;\u0026thinsp;0.14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e6.69\u0026thinsp;\u0026plusmn;\u0026thinsp;5.61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e2.80\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e6\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e11.87\u0026thinsp;\u0026plusmn;\u0026thinsp;0.24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e10.64\u0026thinsp;\u0026plusmn;\u0026thinsp;0.14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e12.75\u0026thinsp;\u0026plusmn;\u0026thinsp;3.66\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e6.82\u0026thinsp;\u0026plusmn;\u0026thinsp;0.07\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e8\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e11.69\u0026thinsp;\u0026plusmn;\u0026thinsp;0.37\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e10.84\u0026thinsp;\u0026plusmn;\u0026thinsp;0.02\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e16.64\u0026thinsp;\u0026plusmn;\u0026thinsp;0.61\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e9.50\u0026thinsp;\u0026plusmn;\u0026thinsp;0.16\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e10\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e11.90\u0026thinsp;\u0026plusmn;\u0026thinsp;0.14\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e11.30\u0026thinsp;\u0026plusmn;\u0026thinsp;0.24\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e19.96\u0026thinsp;\u0026plusmn;\u0026thinsp;4.05\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e12.37\u0026thinsp;\u0026plusmn;\u0026thinsp;0.65\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e12\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c2\"\u003e \u003cp\u003e12.31\u0026thinsp;\u0026plusmn;\u0026thinsp;0.53\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c3\"\u003e \u003cp\u003e12.30\u0026thinsp;\u0026plusmn;\u0026thinsp;0.39\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c4\"\u003e \u003cp\u003e21.10\u0026thinsp;\u0026plusmn;\u0026thinsp;7.20\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"char\" char=\"\u0026plusmn;\" colname=\"c5\"\u003e \u003cp\u003e16.35\u0026thinsp;\u0026plusmn;\u0026thinsp;0.46\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003eThe experimental results in Table\u0026nbsp;\u003cspan refid=\"Tab2\" class=\"InternalRef\"\u003e2\u003c/span\u003e show that the biomass of iron-rich strains supplemented with FeSO4\u0026middot;7H2O and triferric citrate has little difference, but the iron content of cells supplemented with FeSO4\u0026middot;7H2O is higher than that of cells supplemented with triferric citrate. Strain no. F8 can grow in a high iron environment. Finally, The highest biomass and cell iron content were obtained in the medium with 12 mM FeSO4\u0026middot;7H2O concentration.\u003c/p\u003e \u003cp\u003eIn this study, the genome of iron-rich Strain no. F8 was extracted, ITS sequence was amplified, and the strain was identified as Saccharomyces cerevisiae by re-comparison in the database.\u003c/p\u003e \u003cp\u003eYeast is a good carrier of trace elements, which can enrich many trace elements including iron. By means of fermentation technology, adding an appropriate amount of iron ion to the medium can make yeast cells absorb a large amount of iron ion during the proliferation process and bind it to the organic parts of the cells, thus obtaining iron-rich yeast[\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. Relevant studies on iron-rich yeasts have been carried out both at home and abroad, and most of them used Saccharomyces cerevisiae as the carrier of iron enrichment[\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e],In addition, other researchers have used Rhodotorula glutinis[\u003cspan citationid=\"CR20\" class=\"CitationRef\"\u003e20\u003c/span\u003e]、Candida intermedia、Kluyveromyces marxianus[\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e] and so on. Iron-rich yeast can convert inorganic sources of iron into low-toxicity, bioavailable organic iron, which is more beneficial to the human body and has higher biological activity, and is suitable as a new safe iron supplement for the prevention and treatment of iron deficiency anemia[\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e]. In addition, yeast is rich in amino acids, proteins, vitamins and other nutrients[\u003cspan citationid=\"CR21\" class=\"CitationRef\"\u003e21\u003c/span\u003e], and can also be used as a nutritional element supplement, energy and immune enhancer in addition to iron supplementation[\u003cspan citationid=\"CR22\" class=\"CitationRef\"\u003e22\u003c/span\u003e].\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec5\" class=\"Section2\"\u003e \u003ch2\u003e3.2. Body weight change in mice\u003c/h2\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab3\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 3\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eEffects of different treatments on survival rate of mice.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e\u0026nbsp;\u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eNormal control group\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eIDA group\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eFe-Gly group\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eFeSO4 group\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eFe-F8 group\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eDeath\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e0\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e5\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eSurvival rate(%)\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e100%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e100%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e92%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e58%\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e100%\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003e* The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO\u003csub\u003e4\u003c/sub\u003e group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e \u003cp\u003eFigure \u003cspan refid=\"Fig1\" class=\"InternalRef\"\u003e1\u003c/span\u003e shows the growth of mice in each group. There was a large difference in weight gain. The feed intake of the model group was high but the weight of the model group increased more slowly than that of the normal control group, indicating that iron deficiency affected the normal growth of mice. Zheng's study also obtained similar results[\u003cspan citationid=\"CR23\" class=\"CitationRef\"\u003e23\u003c/span\u003e].The yeast group was able to gain weight significantly, which was comparable to the normal control group. However, the overall weight gain trend decreased, which may be due to the small stress response of mice in the early stage, and the weight of mice in the late stage also tended to be saturated. Among them, Fe-Gly group and FeSO4 group lost weight in the later period, and the ratio of feed to gain was significantly higher than other groups.Table\u0026nbsp;\u003cspan refid=\"Tab3\" class=\"InternalRef\"\u003e3\u003c/span\u003e survival rate showed that the FeSO4 group died the most, 5 mice in total, and more lesions were found in their organs after dissection. Other studies have also shown that FeSO4 has certain toxicity, which may be detrimental to mouse growth[\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. Many studies on IDA have shown that IDA affects growth in rats, so our results show that these findings are consistent with previous reports[\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e].\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec6\" class=\"Section2\"\u003e \u003ch2\u003e3.3. Changes of organ coefficient in mice\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab4\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 4\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eOrgan coefficient in the heart, liver, stomach, kidney and spleen of mice in different groups.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"6\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c6\" colnum=\"6\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\" morerows=\"1\" rowspan=\"2\"\u003e \u003cp\u003eGroup\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colspan=\"5\" nameend=\"c6\" namest=\"c2\"\u003e \u003cp\u003eOrgan coefficient\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eHeart\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eLiver\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eStomach\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eKidney\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c6\"\u003e \u003cp\u003eSpleen\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eNormal control group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.53\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e5.31\u0026thinsp;\u0026plusmn;\u0026thinsp;0.41\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.57\u0026thinsp;\u0026plusmn;\u0026thinsp;0.48\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.66\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.29\u0026thinsp;\u0026plusmn;\u0026thinsp;0.07\u003csup\u003eab\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eIDA group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.59\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e5.03\u0026thinsp;\u0026plusmn;\u0026thinsp;0.42\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.83\u0026thinsp;\u0026plusmn;\u0026thinsp;0.13\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.64\u0026thinsp;\u0026plusmn;\u0026thinsp;0.18\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.31\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-Gly group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.56\u0026thinsp;\u0026plusmn;\u0026thinsp;0.01\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e5.01\u0026thinsp;\u0026plusmn;\u0026thinsp;0.39\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2.15\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.51\u0026thinsp;\u0026plusmn;\u0026thinsp;0.19\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.26\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFeSO4 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.55\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e4.79\u0026thinsp;\u0026plusmn;\u0026thinsp;0.40\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e2.13\u0026thinsp;\u0026plusmn;\u0026thinsp;0.36\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.51\u0026thinsp;\u0026plusmn;\u0026thinsp;0.09\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.19\u0026thinsp;\u0026plusmn;\u0026thinsp;0.05\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-F8 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e0.57\u0026thinsp;\u0026plusmn;\u0026thinsp;0.11\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e5.19\u0026thinsp;\u0026plusmn;\u0026thinsp;0.59\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e1.70\u0026thinsp;\u0026plusmn;\u0026thinsp;0.69\u003csup\u003ebc\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e1.58\u0026thinsp;\u0026plusmn;\u0026thinsp;0.21\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c6\"\u003e \u003cp\u003e0.28\u0026thinsp;\u0026plusmn;\u0026thinsp;0.06\u003csup\u003ebc\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003e \u003csup\u003e*\u003c/sup\u003e The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO\u003csub\u003e4\u003c/sub\u003e group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e \u003cp\u003eAs shown in Table\u0026nbsp;\u003cspan refid=\"Tab4\" class=\"InternalRef\"\u003e4\u003c/span\u003e, the heart coefficient in IDA group was significantly higher than that in Normal control group (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). In addition, after supplementation of Fe-Gly, FeSO4 and Fe-F8, the heart coefficient showed some improvement, This result was also observed by Zhang et al[\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e]. In iron-deficient organisms, liver mass and volume are significantly reduced, which is typical of iron deficiency. The organ coefficients of the livers in iron enriched supplemented animals were lower than that of iron-deficient animals. Table\u0026nbsp;\u003cspan refid=\"Tab4\" class=\"InternalRef\"\u003e4\u003c/span\u003e Organ index showed that spleen weight of mice in iron deficiency group was significantly higher than that in other groups (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). This result was also observed by Yun et al[\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e].Kidney index showed no significant difference, and some studies showed that iron deficiency had no significant effect on spleen weight.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec7\" class=\"Section2\"\u003e \u003ch2\u003e3.4. HE section of liver and kidney tissue\u003c/h2\u003e \u003cp\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003eThe intestinal barrier plays an important role in the health of the body as it is the main immune, digestive and nutrient absorbing tissue. Colon tissue sections were obtained by H\u0026amp;E staining to further study the effect of iron supplementation on the intestinal tract of IDA mice.As shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig2\" class=\"InternalRef\"\u003e2\u003c/span\u003e, the mucosal epithelial cells of the colon tissue of mice in the normal control group were closely arranged and orderly, the lamina propria was intact, and the submucosal tissue was intact, with a large number of well-structured, long and clear crypts and villi, and the muscle fibers were neatly arranged and no lesions occurred.Compared with the normal control group, the colonic tissue of mice in IDA group had obvious changes in the overall structure, including mucosal epithelial cell arrangement defect, lamina propria atrophy, crypt structure damage, and epithelial barrier deformation. Wang et al. showed similar results in the colons of IDA mice[\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e].IDA reduces the antioxidant capacity of the body, leading to structural abnormalities and dysfunction of the organism biofilm, causing damage and inflammation of colon epithelial cells.\u003c/p\u003e \u003cp\u003eAfter 4w of intragastric administration, part of the acines in the colon tissue of the FeSO4 group showed necrosis and defect and lamina propria atrophy, but the submucosal tissue was intact, the muscle fibers were arranged neatly and no lesions occurred.The colonic tissue structure of mice in Fe-Gly group and Fe-F8 group was orderly, the mucosa tended to be normal, the lamina propria was intact, the crypt structure basically returned to normal, inflammatory cell infiltration basically disappeared, the gap between the mucosa and the lower layer was significantly reduced, and the muscle fibers were neatly arranged without any lesions.These results indicate that supplementation of a certain dose of iron can effectively improve the intestinal mucosal barrier damage and inflammatory cell infiltration caused by IDA, among which Fe-Gly and Fe-F8 have better improvement effects than FeSO4 at the same dose. Wang's studies on other peptide iron chelates have also obtained similar results[\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e], In the FeSO4 group, intestinal tissue was damaged, a large number of mucosal epithelial cells were shed, villi erosion was observed, intestinal mucosa in the Marine fish skin peptide iron group, casein peptide iron group and whey protein group was improved, and the villi were intact and the glands were normal.\u003c/p\u003e \u003cp\u003eFigure \u003cspan refid=\"Fig3\" class=\"InternalRef\"\u003e3\u003c/span\u003e The results of liver morphology of mice in each group showed that, compared with the normal control group, the hepatocyte cords around the central vein of the liver in IDA group were not clearly demarcated and arranged in a disorderly manner.After 4w of intragastric administration, the liver color of Fe-F8 group gradually turned red, and the lines of hepatocyte cords around the central vein were clear, and the arrangement tended to be neat. However, the cells in Fe-Gly group and FeSO4 group were improved to a certain extent, but the cord boundaries of hepatocytes were still unclear and the arrangement was chaotic.In conclusion, supplementation with a certain dose of Fe-F8 can significantly improve the damage of intestinal mucosal barrier and inflammatory cell infiltration caused by IDA, as well as the arrangement of liver cells, and the effect is better than Fe-Gly and FeSO4.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec8\" class=\"Section2\"\u003e \u003ch2\u003e3.5. Hemoglobin level\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003eHemoglobin levels before and after the trial are shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig4\" class=\"InternalRef\"\u003e4\u003c/span\u003e. Hemoglobin is a red blood cell protein responsible for transporting oxygen into tissues, where iron has a central place in the hemoglobin structure, IDA can cause hemoglobin to decline[\u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eDue to iron deficiency diet, the hemoglobin content of the model group was the lowest, which was significantly lower than that of the normal control group (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), Hb 83\u0026thinsp;~\u0026thinsp;84 g/L, belonging to the anemia level (less than 90g/L).After feeding iron supplement (Fe-Gly, FeSO4 and Fe-F8) for 4w, the hemoglobin level of the three iron supplement groups recovered, and the Hb content of the three iron supplement groups had no significant difference compared with the normal control group at the beginning of the experiment (P\u0026thinsp;\u0026gt;\u0026thinsp;0.05).Among them, the hemoglobin content of Fe-F8 group can be restored to the original level of normal control group, and the results confirmed that yeast iron can promote the recovery of hemoglobin content.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec9\" class=\"Section2\"\u003e \u003ch2\u003e3.6. Red blood cell count(RBC) and hematocrit level(HCT)\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab5\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 5\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eHematological parameters of mice in different groups.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"5\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c5\" colnum=\"5\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eGroup\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eHb(g/L)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eRBC(*10\u003csup\u003e12\u003c/sup\u003e*L\u003csup\u003e\u0026minus;\u0026thinsp;1\u003c/sup\u003e)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eHCT(%)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c5\"\u003e \u003cp\u003eMCV(fL)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eNormal control group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e139.93\u0026thinsp;\u0026plusmn;\u0026thinsp;5.56\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e7.03\u0026thinsp;\u0026plusmn;\u0026thinsp;0.61\u003csup\u003ebc\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e40.24\u0026thinsp;\u0026plusmn;\u0026thinsp;2.13\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e70.12\u0026thinsp;\u0026plusmn;\u0026thinsp;5.19\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eIDA group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e84.91\u0026thinsp;\u0026plusmn;\u0026thinsp;3.89\u003csup\u003ee\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e6.43\u0026thinsp;\u0026plusmn;\u0026thinsp;0.47\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e30.12\u0026thinsp;\u0026plusmn;\u0026thinsp;1.94\u003csup\u003ee\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e64.42\u0026thinsp;\u0026plusmn;\u0026thinsp;4.38\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-Gly group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e111.57\u0026thinsp;\u0026plusmn;\u0026thinsp;3.81\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e7.91\u0026thinsp;\u0026plusmn;\u0026thinsp;0.37\u003csup\u003eab\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e36.27\u0026thinsp;\u0026plusmn;\u0026thinsp;2.21\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e72.36\u0026thinsp;\u0026plusmn;\u0026thinsp;6.01\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFeSO4 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e102.55\u0026thinsp;\u0026plusmn;\u0026thinsp;4.13\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e7.01\u0026thinsp;\u0026plusmn;\u0026thinsp;0.24\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e34.23\u0026thinsp;\u0026plusmn;\u0026thinsp;2.72\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e69.19\u0026thinsp;\u0026plusmn;\u0026thinsp;4.51\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-F8 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e120.21\u0026thinsp;\u0026plusmn;\u0026thinsp;6.74\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e8.04\u0026thinsp;\u0026plusmn;\u0026thinsp;0.53\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e43.88\u0026thinsp;\u0026plusmn;\u0026thinsp;3.10\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c5\"\u003e \u003cp\u003e75.27\u0026thinsp;\u0026plusmn;\u0026thinsp;6.18\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003e \u003csup\u003e*\u003c/sup\u003e The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO\u003csub\u003e4\u003c/sub\u003e group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e \u003cp\u003eHemoglobin is a pigment protein present in red blood cells. The main physiological function of RBC is realized through hemoglobin, transporting oxygen to the body's tissue cells to produce energy for metabolism. Hematocrit (HCT) reflects the proportion of red blood cells in the whole blood[\u003cspan citationid=\"CR30\" class=\"CitationRef\"\u003e30\u003c/span\u003e].When iron intake is insufficient to meet daily needs, the body's iron stores drop too low to support normal red blood cell production, resulting in IDA, followed by low HCT, MCV, MCH, or MCHC[\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e].\u003c/p\u003e \u003cp\u003eThe data in Table\u0026nbsp;\u003cspan refid=\"Tab5\" class=\"InternalRef\"\u003e5\u003c/span\u003e show that after 4 weeks of recovery experiment, Hb of the anemia mice supplemented with iron-rich yeast recovers to 120.21 g/L, which is significantly different from that of the other two iron supplement groups, while the hemoglobin content of the Fe-Gly and FeSO4 groups is still lower than that of the normal control group.In addition, at the end of this experiment, RBC, HCT and MCV parameters of Fe-Gly group, FeSO4 group and Fe-F8 group were significantly higher than those of IDA group (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). It is worth noting that RBC, HCT and MCV levels in Fe-F8 group were significantly higher than those in Fe-Gly group and FeSO4 group, indicating that Fe-F8 was more effective in improving anemia symptoms.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec10\" class=\"Section2\"\u003e \u003ch2\u003e3.7. Serum iron level\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003eThe changes of serum iron were shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig5\" class=\"InternalRef\"\u003e5\u003c/span\u003e. The serum iron concentration in IDA group was the lowest and the highest in Fe-F8 group, which was significantly different from Fe-Gly group and FeSO4 group (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). In this study, the SI level in the IDA group was significantly lower than that in the normal control group (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), which was similar to the result observed by C. Xiao et al[\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e]. The results showed that yeast iron could promote the recovery of serum iron content.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec11\" class=\"Section2\"\u003e \u003ch2\u003e3.8. Serum iron level\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab6\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 6\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eThe changes of serum immunoglobulin content in mice.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"3\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eGroup\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eIgG (g/L)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eIgM (g/L)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eNormal control group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e17.59\u0026thinsp;\u0026plusmn;\u0026thinsp;0.48\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e3.16\u0026thinsp;\u0026plusmn;\u0026thinsp;0.12\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eIDA group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e11.76\u0026thinsp;\u0026plusmn;\u0026thinsp;0.62\u003csup\u003ee\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e2.65\u0026thinsp;\u0026plusmn;\u0026thinsp;0.08\u003csup\u003ee\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-Gly group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e22.55\u0026thinsp;\u0026plusmn;\u0026thinsp;2.12\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e4.72\u0026thinsp;\u0026plusmn;\u0026thinsp;0.19\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFeSO4 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e14.93\u0026thinsp;\u0026plusmn;\u0026thinsp;0.31\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e5.01\u0026thinsp;\u0026plusmn;\u0026thinsp;0.23\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-F8 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e24.68\u0026thinsp;\u0026plusmn;\u0026thinsp;2.37\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e6.06\u0026thinsp;\u0026plusmn;\u0026thinsp;0.29\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003e \u003csup\u003e*\u003c/sup\u003e The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO\u003csub\u003e4\u003c/sub\u003e group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e \u003cp\u003eImmunoglobulins are major players in humoral immunity. IgG is the most abundant immunoglobulin in the body, which is mainly responsible for neutralizing toxins and regulating immune cell function, while IgM is mainly distributed in the blood and is the first antibody to appear in the initial immune response[\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e]. After 4 weeks of iron supplementation, the contents of immunoglobulin IgG and IgM in the blood of mice were measured by anatomic blood collection, and the results were shown in Table\u0026nbsp;\u003cspan refid=\"Tab6\" class=\"InternalRef\"\u003e6\u003c/span\u003e.\u003c/p\u003e \u003cp\u003eThe IgG and IgM contents of mice in IDA group were significantly lower than those in Normal control group (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05). The IgG and IgM contents in iron supplementation group were significantly different from those in Normal control group, indicating that supplementation with a certain dose of iron could improve the immune status of IDA mice, and it was noteworthy that Fe-F8 group was the highest (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05).\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec12\" class=\"Section2\"\u003e \u003ch2\u003e3.9. Antioxidant enzyme activity and MDA level and total antioxidant capacity\u003c/h2\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003eThe total antioxidant activity and MDA levels of mice in each group were shown in Fig.\u0026nbsp;\u003cspan refid=\"Fig6\" class=\"InternalRef\"\u003e6\u003c/span\u003e and Table\u0026nbsp;\u003cspan refid=\"Tab6\" class=\"InternalRef\"\u003e6\u003c/span\u003e. The results showed that compared with the model group, Fe-rich yeast could improve the total antioxidant capacity in serum of mice. The total antioxidant capacity of yeast group was 53.31%, which was higher than that of other experimental groups, but slightly lower than that of Normal control group.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"gridtable\"\u003e\u003ctable float=\"Yes\" id=\"Tab7\" border=\"1\"\u003e \u003ccaption language=\"En\"\u003e \u003cdiv class=\"CaptionNumber\"\u003eTable 7\u003c/div\u003e \u003cdiv class=\"CaptionContent\"\u003e \u003cp\u003eThe activities of antioxidant enzymes and the MDA level of mice in different groups.\u003c/p\u003e \u003c/div\u003e \u003c/caption\u003e \u003ccolgroup cols=\"4\"\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c1\" colnum=\"1\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c2\" colnum=\"2\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c3\" colnum=\"3\"\u003e\u003c/div\u003e \u003cdiv align=\"left\" class=\"colspec\" colname=\"c4\" colnum=\"4\"\u003e\u003c/div\u003e \u003cthead\u003e \u003ctr\u003e \u003cth align=\"left\" colname=\"c1\"\u003e \u003cp\u003eGroup\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c2\"\u003e \u003cp\u003eSOD (U/ml)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c3\"\u003e \u003cp\u003eGSH-PX (U/ml)\u003c/p\u003e \u003c/th\u003e \u003cth align=\"left\" colname=\"c4\"\u003e \u003cp\u003eMDA (umol/ml)\u003c/p\u003e \u003c/th\u003e \u003c/tr\u003e \u003c/thead\u003e \u003ctbody\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eNormal control group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e151.90\u0026thinsp;\u0026plusmn;\u0026thinsp;3.53\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e598.84\u0026thinsp;\u0026plusmn;\u0026thinsp;24.49\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e24.98\u0026thinsp;\u0026plusmn;\u0026thinsp;2.45\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eIDA group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e100.55\u0026thinsp;\u0026plusmn;\u0026thinsp;2.34\u003csup\u003ee\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e464.77\u0026thinsp;\u0026plusmn;\u0026thinsp;23.23\u003csup\u003ed\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e16.07\u0026thinsp;\u0026plusmn;\u0026thinsp;1.91\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-Gly group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e187.90\u0026thinsp;\u0026plusmn;\u0026thinsp;4.25\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e662.74\u0026thinsp;\u0026plusmn;\u0026thinsp;28.13\u003csup\u003eb\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e17.55\u0026thinsp;\u0026plusmn;\u0026thinsp;1.77\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFeSO4 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e157.80\u0026thinsp;\u0026plusmn;\u0026thinsp;3.57\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e599.94\u0026thinsp;\u0026plusmn;\u0026thinsp;29.08\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e16.87\u0026thinsp;\u0026plusmn;\u0026thinsp;1.19\u003csup\u003ec\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003ctr\u003e \u003ctd align=\"left\" colname=\"c1\"\u003e \u003cp\u003e\u003cb\u003eFe-F8 group\u003c/b\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c2\"\u003e \u003cp\u003e198.06\u0026thinsp;\u0026plusmn;\u0026thinsp;8.61\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c3\"\u003e \u003cp\u003e669.90\u0026thinsp;\u0026plusmn;\u0026thinsp;30.17\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003ctd align=\"left\" colname=\"c4\"\u003e \u003cp\u003e35.51\u0026thinsp;\u0026plusmn;\u0026thinsp;3.07\u003csup\u003ea\u003c/sup\u003e\u003c/p\u003e \u003c/td\u003e \u003c/tr\u003e \u003c/tbody\u003e \u003c/colgroup\u003e \u003c/table\u003e\u003c/div\u003e \u003c/p\u003e \u003cp\u003e \u003cdiv class=\"BlockQuote\"\u003e \u003cp\u003e \u003csup\u003e*\u003c/sup\u003e The superscript of the data in the table was obtained through ANOVA test analysis, and different letter superscripts indicated significant differences in the measured substance content (p\u0026thinsp;\u0026lt;\u0026thinsp;0.05).The IDA group was not treated, normal control group was treated with standard granule diet, Fe-Gly group was treated with ferrous glycine (3 mg iron), FeSO\u003csub\u003e4\u003c/sub\u003e group was treated with ferrous sulfate (3 mg iron), and Fe-F8 group was treated with yeast iron (3 mg iron), respectively.\u003c/p\u003e \u003cp\u003eCompared with normal control group or iron supplement group, the activities of SOD and GSH-Px in IDA group were significantly decreased (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), and the level of MDA in IDA group was also the lowest. In addition, the activities of SOD and GSH-Px in Fe-F8 group were higher than those in other iron supplement groups (P\u0026thinsp;\u0026lt;\u0026thinsp;0.05), and the activities of SOD and GSH-Px in all groups were the highest. However, there was no significant difference in MDA concentration between FeSO4 group and IDA group. The experimental results show that the improvement effect of Fe-F8 group is more significant than that of Fe-Gly and FeSO4.Tang's research results show that compared with FeSO4, heme iron-enriching peptides can enhance the activity of antioxidant.\u003c/p\u003e \u003c/div\u003e \u003c/p\u003e \u003c/div\u003e"},{"header":"4. Discussion","content":"\u003cp\u003eIn this study, a strain F8 with iron-rich potential was selected. On this basis, the improvement effect of iron-rich yeast on iron-deficiency anemia was studied by using the iron deficiency anemia model of mice.Our results show that Fe-F8 can improve IDA in mice by weight, hematological parameters, organ parameters and antioxidant activity in vivo, We found that Fe-F8 has a more significant effect on IDA, with higher bioavailability and fewer side effects compared to Fe-Gly and FeSO4.This finding suggests that iron-rich yeast has great potential as an effective source of iron supplementation in IDA mice, and the effects of Strain no. F8 on other animals or humans need to be further studied.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cul\u003e\n \u003cli\u003eFunding: This research was funded by the National Key R\u0026amp;D Program of China (no.2021YFD1301000).\u003c/li\u003e\n \u003cli\u003eConflicts of Interest:\u0026nbsp;All the authors declare no conflict of interest.\u003c/li\u003e\n \u003cli\u003eEthics approval：In accordance with the requirements of the National Act on the use of laboratory animals (People\u0026apos;s Republic of China), animal use in current research concerns after evaluation and accreditation of the Association for the Care of Laboratory Animals (AAALAC) and Animal testing protocols are approved by the Animal Ethics Committee.\u003c/li\u003e\n \u003cli\u003eAuthor Contributions: Conceptualization, Ying Chen; methodology, Hongbinig Wan; software, Yuanxiang Pang; validation and formal analysis, Hongbing Wan, Yuanxiang Pang; investigation, Xinyi Zhou; resources, Mingli Wan,Shengshuo Li; data curation, Ying Chen; writing\u0026mdash;original draft preparation, Ying Chen; writing\u0026mdash;review and editing, Ying Chen; visualization, Xuelian Liu; supervision, Xuelian Liu; project administration, Xuelian Liu. All authors have read and agreed to the published version of the manuscript.\u003c/li\u003e\n\u003c/ul\u003e"},{"header":"References","content":"\u003col\u003e\n \u003cli\u003eDallman P R. Biochemical Basis for the Manifestations of Iron Deficiency.\u003cem\u003eAnn Rev Nutr, 2003, 6(1):13-40\u003c/em\u003e.\u003c/li\u003e\n \u003cli\u003eHassan T H;\u0026nbsp;Badr M A; Karam N A; et al. Impact of iron deficiency anemia on the function of the immune system in children. \u003cem\u003eMedicine，2016，95(47): e5395\u003c/em\u003e.\u003c/li\u003e\n \u003cli\u003eDuque X; Martinez H; Vilchis-Gil J; et al. Effect of supplementation with ferrous sulfate or iron bis-glycinate chelate on ferritin concentration in Mexican schoolchildren: a randomized controlled trial.\u003cem\u003eNutrition Journal, 2014, 13(1):71\u003c/em\u003e.\u003c/li\u003e\n \u003cli\u003eBenoist B; McLean E; Egll I; et al. 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Effect of iron supplementation on immune function in patients with iron deficiency anemia. Shandong Medicine, 2014, 000(020):68-69.\u003c/li\u003e\n \u003cli\u003eTang N; Chen L Q; Zhuang H. Effects of heme iron enriched peptide on iron deficiency anemia in rats. Food \u0026amp; Function, 2014, 5(2):390-399. \u0026nbsp; \u0026nbsp;\u003c/li\u003e\n\u003c/ol\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"biometals","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"biom","sideBox":"Learn more about [BioMetals](http://link.springer.com/journal/10534)","snPcode":"10534","submissionUrl":"https://submission.nature.com/new-submission/10534/3","title":"BioMetals","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Iron deficiency anemia, Mice, Ferriferous yeast, Hematological parameters, Antioxidant activity","lastPublishedDoi":"10.21203/rs.3.rs-3769317/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3769317/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003eIron deficiency anemia (IDA) is one of the most serious forms of malnutrition.Wild type strains of Saccharomyces cerevisiae have higher tolerance to inorganic iron and higher iron conversion and accumulation capacity.The aim of this study was to investigate the effect of yeast iron as a potential organic iron supplement on mice with iron deficiency anemia.60 male KM mice were randomly divided into normal control group and iron deficiency diet model group to establish IDA model. After the model was established, IDA mice were randomly divided into 5 groups: normal control group, IDA group, organic iron (Fe-Gly) group, inorganic iron (FeSO4) group and yeast iron (Fe-F8) group.Mice in the experimental group were given different kinds of iron by intragastric administration once a day for 4w.The results showed that Fe-F8 had an effective recovery function, and the body weight and hematological parameters of IDA mice returned to normal levels. The activities of malondialdehyde, superoxide dismutase, glutathione peroxidase and total antioxidant capacity in serum were increased.In addition, Fe-F8 was more effective in alleviating IDA and improving organ indices with fewer side effects compared to ferrous glycinate and ferrous sulfate groups. This study suggests that the iron-rich strain F8 may play an important role in improving IDA mice and may be developed as a new iron supplement.\u003c/p\u003e","manuscriptTitle":"Production of Iron-enriched Yeast and it’s application in the treatment of iron-deficiency anemia","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-01-01 08:42:50","doi":"10.21203/rs.3.rs-3769317/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Revision requested","date":"2024-01-19T15:49:47+00:00","index":"","fulltext":""},{"type":"editorInvitedReview","content":"","date":"2024-01-14T09:48:34+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"0d220ac4-0f66-41ea-917e-96eaf22d583c","date":"2023-12-31T09:30:56+00:00","index":"hide","fulltext":""},{"type":"reviewerAgreed","content":"fe300264-5742-40a9-a477-2b79ff862f33","date":"2023-12-31T08:02:32+00:00","index":"hide","fulltext":""},{"type":"reviewersInvited","content":"","date":"2023-12-31T00:31:16+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2023-12-27T12:25:07+00:00","index":"","fulltext":""},{"type":"checksComplete","content":"","date":"2023-12-27T12:25:07+00:00","index":"","fulltext":""},{"type":"submitted","content":"BioMetals","date":"2023-12-18T00:53:06+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"biometals","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"biom","sideBox":"Learn more about [BioMetals](http://link.springer.com/journal/10534)","snPcode":"10534","submissionUrl":"https://submission.nature.com/new-submission/10534/3","title":"BioMetals","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"db66d405-9b18-49cd-869b-dc30014ce1b4","owner":[],"postedDate":"January 1st, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-04-01T15:07:30+00:00","versionOfRecord":{"articleIdentity":"rs-3769317","link":"https://doi.org/10.1007/s10534-024-00592-3","journal":{"identity":"biometals","isVorOnly":false,"title":"BioMetals"},"publishedOn":"2024-03-28 15:01:47","publishedOnDateReadable":"March 28th, 2024"},"versionCreatedAt":"2024-01-01 08:42:50","video":"","vorDoi":"10.1007/s10534-024-00592-3","vorDoiUrl":"https://doi.org/10.1007/s10534-024-00592-3","workflowStages":[]},"version":"v1","identity":"rs-3769317","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3769317","identity":"rs-3769317","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}