Development of experimental endometriosis is associated with the level of implant miR-451 expression.

Experimental endometriosis was induced and development assessed as described under “Materials and Methods”. A. The number of implants of each genotype that developed in wild-type host mice (N = 8/genotype). Data are presented as the mean ± SEM and were analyzed by one-way ANOVA followed by post-hoc analysis. Different letters indicate statistical significance (P<0.05). B. Elevated miR-451 expression is associated with wild-type “implant” tissue that develops ectopically. Uterine fragments were obtained from wild-type mice at the time (0 h) of PMSG administration or 44 h later (the time of endometrial fragment harvest). miR-451 and miR-144 expression was determined by qRT-PCR. Different letters indicate statistical significance (P<0.05) between groups (by unpaired t-test). ND indicates that miR-144 levels were not detectable by qRT-PCR.