Development Of A Novel Light-Up Probe For Detection Of G-Quadruplexes In Stress Granules

Development Of A Novel Light-Up Probe For Detection Of G-Quadruplexes In Stress Granules | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Development Of A Novel Light-Up Probe For Detection Of G-Quadruplexes In Stress Granules Keisuke Iida, Ayano Sasaki, Shunsuke Ishida, Takayoshi Arai This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1341045/v1 This work is licensed under a CC BY 4.0 License Status: Under Review Version 1 posted 8 You are reading this latest preprint version Abstract G-quadruplexes (G4s) regulate various biological processes in cells. However, cellular imaging of dynamically forming G4s in biomolecular condensates using small molecules has been poorly investigated. Herein, we present a fluorescent light-up probe with the ability to selectively stabilize G4s and enhance fluorescence upon G4 binding. The foci of the probe were mainly observed in the nucleoli. These were co-localized with anti-fibrillarin antibodies and anti-G4 antibodies (BG4). Moreover, we tested detection of G4 in stress granules using the developed probe. Stress granules were induced through treatment with not only thapsigargin, but also known G4 ligands (pyridostatin, RHPS4, and BRACO-19). In the stress granules, co-localization between the probe, BG4, and stress granule markers (TIA1 and G3BP1) was detected. We present a practical light-up probe for G4s in stress granules, providing potential targets for G4 ligands. Full Text Additional Declarations No competing interests reported. Supplementary Files 220214supprementaryimformation.pdf Cite Share Download PDF Status: Under Review Version 1 posted Editorial decision: Major revision 17 Mar, 2022 Reviews received at journal 04 Mar, 2022 Reviewers agreed at journal 22 Feb, 2022 Reviewers invited by journal 22 Feb, 2022 Editor assigned by journal 22 Feb, 2022 Editor invited by journal 22 Feb, 2022 Submission checks completed at journal 22 Feb, 2022 First submitted to journal 08 Feb, 2022 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1341045","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":85694644,"identity":"7b70ddc1-f642-4ac8-8a11-92ea8e0d218a","order_by":0,"name":"Keisuke Iida","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAAvUlEQVRIiWNgGAWjYBACCWbGBgmGCoYENgkwn41YLWdI0gLCjG0MCQwSxDpMsp258cbPeXZ5fNLND5h5GPjyCGqRZmZstuzdllzMJnPMAKiFrZigFjlmxjYJ3m0HEtskEsx/A7UkNhCjRfLvHJCW9A/MRGkBOqxNmrcBpCXHgDgtks2MzdYyx5IT22TOFDDOMSDCLxLnjz+8+abGLnH+7PYNDG8qjhEOMTRgcCyBVC0MNaRrGQWjYBSMgmEPAJ73M2gkFfMsAAAAAElFTkSuQmCC","orcid":"","institution":"Chiba Iodine Resource Innovation Center (CIRIC), Chiba University","correspondingAuthor":true,"prefix":"","firstName":"Keisuke","middleName":"","lastName":"Iida","suffix":""},{"id":85694646,"identity":"cc9e9eeb-522a-4151-bab1-a39aea8cc25a","order_by":1,"name":"Ayano Sasaki","email":"","orcid":"","institution":"Chiba Iodine Resource Innovation Center (CIRIC), Chiba University","correspondingAuthor":false,"prefix":"","firstName":"Ayano","middleName":"","lastName":"Sasaki","suffix":""},{"id":85694647,"identity":"22644110-04a6-4c36-b945-9f93d7ccaa01","order_by":2,"name":"Shunsuke Ishida","email":"","orcid":"","institution":"Chiba Iodine Resource Innovation Center (CIRIC), Chiba University","correspondingAuthor":false,"prefix":"","firstName":"Shunsuke","middleName":"","lastName":"Ishida","suffix":""},{"id":85694648,"identity":"eb17f443-90c7-4ab5-8987-18ff64b9672b","order_by":3,"name":"Takayoshi Arai","email":"","orcid":"","institution":"Chiba Iodine Resource Innovation Center (CIRIC), Chiba University","correspondingAuthor":false,"prefix":"","firstName":"Takayoshi","middleName":"","lastName":"Arai","suffix":""}],"badges":[],"createdAt":"2022-02-09 04:14:05","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-1341045/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-1341045/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":18530062,"identity":"52a398ac-5ef8-4ffb-98a0-1910bdb549b2","added_by":"auto","created_at":"2022-02-23 16:00:15","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":557064,"visible":true,"origin":"","legend":"","description":"","filename":"220222manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1341045/v1_covered.pdf"},{"id":18530058,"identity":"81d7d036-9376-475e-8518-b95fd832cb96","added_by":"auto","created_at":"2022-02-23 16:00:03","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"supplement","size":7239589,"visible":true,"origin":"","legend":"","description":"","filename":"220214supprementaryimformation.pdf","url":"https://assets-eu.researchsquare.com/files/rs-1341045/v1/a401910b3e338d8e18760b8c.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"\u003cp\u003eDevelopment Of A Novel Light-Up Probe For Detection Of G-Quadruplexes In Stress Granules\u003c/p\u003e","fulltext":[{"header":"Full Text","content":"This preprint is available for \u003ca href='/article/rs-1341045/latest.pdf' target='_blank'\u003edownload as a PDF\u003c/a\u003e."}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"scientific-reports","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"scirep","sideBox":"Learn more about [Scientific Reports](http://www.nature.com/srep/)","snPcode":"","submissionUrl":"","title":"Scientific Reports","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"stoa","reportingPortfolio":"Scientific Reports","inReviewEnabled":true,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-1341045/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-1341045/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"G-quadruplexes (G4s) regulate various biological processes in cells. 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