Performance Evaluation Of Bacterial Consortium For Biodegradation Of Total Petroleum Hydrocarbon: A Comparative Strategic Biostimulation Study

Performance Evaluation Of Bacterial Consortium For Biodegradation Of Total Petroleum Hydrocarbon: A Comparative Strategic Biostimulation Study | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Performance Evaluation Of Bacterial Consortium For Biodegradation Of Total Petroleum Hydrocarbon: A Comparative Strategic Biostimulation Study Ipsita Dipamitra Behera, Bhim Charan Meikap, Ramkrishna Sen This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-1227394/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract This research work demonstrated the comparative study of the efficacy of poultry litter extract (PLE) and inorganic fertilizer (NPK) as biostimulating agents for enhancing total petroleum hydrocarbon (TPH) degradation of petroleum refinery sludge (PRS) with bioaugmentation of an indigenously developed bacterial consortium. In this study, six sets of treatments such as natural attenuation, bioaugmentation with the indigenously developed bacterial consortium, and various biostimulation strategies with (MSM100, PLE100, NPK100, MSM50+PLE50, and MSM50+NPK50) were performed to meet 100% nutrient source for bacterial growth to enhance TPH degradation. Among all, the combined sources of MSM50+PLE50 showed the best performance by degrading the TPH up to 91.3 ± 4.1% within 28 d of the incubation period. The GC-FID analysis confirmed the efficacy of TPH degradation of PRS when PLE amendment with MSM. Further, the removal of maltene and asphaltene was also achieved 92 ± 3.7% and 52 ± 2.2% during this treatment. TPH degradation fitted to first-order kinetics with a rate constant 0.09 d -1 and half-life period of 7.7 d for MSM50+PLE50 amendment treatment along with bacterial consortium as bioaugmentation. This study revealed the implementation of the PLE amendment not only preferred as a nutrient source for bacterial growth but also enhanced TPH biodegradation in an eco-friendly strategic way by dropping the practice of inorganic salts. Petroleum sludge Total petroleum hydrocarbon (TPH) Bacterial consortium Biodegradation Amendment. Full Text Supplementary Files Supplementarymaterials.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-1227394","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":82541502,"identity":"fa56a362-a675-4dcf-9f21-b34744a68165","order_by":0,"name":"Ipsita Dipamitra 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