Expression of microRNA-26b-5p is related to the changes of random blood glucose after primary percutaneous coronary interventions

Abstract Background: Stress hyperglycemia （SH） is prevalent in non-diabetic patients with ST-segment elevation myocardial infarction (STEMI), and it can significantly increase the risk of adverse cardiovascular events. The study aims to determine the changes of and correlation between circulating microRNA-26b-5p levels and random blood glucose levels after primary percutaneous coronary interventions (p-PCI). Methods: INS-1 cells were used to establish model of insulin regulation. Overexpression of miR-26b was performed with lentiviral transfection, while downregulation of miR-26b was accomplished by using shRNA sequences. Insulin was quantitatively detected by an enzyme-linked immunosorbent assay kit (ELISA).we enrolled 48 patients with acute anterior wall myocardial infarction who successfully underwent p-PCI from June 2017 to November 2017 in beijing anzhen hospital and beijing friendship hospital, capital medical university. Plasma samples were collected before p-PCI, 24h and 72h after operation. The level of circulating microRNA-26b-5p were measured by Real Time PCR. The association between circulating microRNA-26b-5p and random blood glucose was calculated by Spearman correlation coefficient. Results: The results of in vitro test showed i nhibition of miR-26b leads to a decrease in insulin secretion while overexpression of miR-26b increases insulin secretion in INS-1 cells. 34 male patients accounting for 71% were included in this study. The circulating microRNA-26b-5p levels were 14.41±8.52 and 19.63±9.42 at 24h and 72h after operation respectively, as compared with 10.32±6.62 before p-PCI ( P <0.001). Significantly decreased random blood glucose level were found at 24h and 72h after operation compared with that before p-PCI（7.35±1.17 VS. 7.12±1.17 VS. 8.28±1.35, P< 0.001. Spearman correlation coefficient revealed that there was an inverse correlation between circulating microRNA-26b-5p levels and random blood glucose levels（ r =-0.425 ， P <0.001). Conclusion: MicroRNA-26b-5p may be involved in the regulation of blood glucose in non-diabetic patients with STEMI after p-PCI.