Impact of epicatechin supplementation on plasma proteome profiles in obese men and women -- an exploratory approach to sexual dimorphism

Impact of epicatechin supplementation on plasma proteome profiles in obese men and women -- an exploratory approach to sexual dimorphism | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL Clinical Applications This is a preprint and has not been peer reviewed. Data may be preliminary. 14 April 2025 V1 Latest version Share on Impact of epicatechin supplementation on plasma proteome profiles in obese men and women -- an exploratory approach to sexual dimorphism Authors : Celso Pereira Batista Sousa-Filho , Allanis Ferreira Valon , Talita Souza Siqueira , Leo Iwai 0000-0002-1571-7763 , Alison Felipe Alencar Chaves , Victoria Silva 0000-0002-6253-0387 , Guilherme Ribeiro Romualdo , and Rosemari Otton 0000-0001-5503-3967 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.174461959.94166946/v1 Published PROTEOMICS – Clinical Applications Version of record Peer review timeline 362 views 159 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Evidence suggests that consuming epicatechin-rich green tea can increase metabolism in the body, and this metabolic effect might be linked to weight loss in obese subjects. The precise mechanism by which epicatechin influences weight loss is still unclear. Our goal was to identify a specific signature in the plasma proteins of obese individuals, categorized or not by gender (men and women), and to investigate how epicatechin (EC) supplementation affected them. Additionally, we analyzed anthropometric data to assess the potential anti-obesity effects of EC and to identify any gender-related differences that may have emerged. In our clinical trial, we provided pure EC (90%) at a daily dosage of 200 mg, administered before the main meal, for three months. The participants were obese men and women with a body mass index (BMI) of 30 kg/m² or higher. We conducted measurements of body dimensions and performed biochemical blood tests before and after the supplementation with EC, also analysing the proteome in the plasma samples. EC supplementation did not alter anthropometric parameters in obese subjects, but it did cause significant molecular changes in their plasma proteome, which varied between men and women. Key proteins like RPL30 were consistently regulated, indicating that EC might activate translational remodeling to adapt to metabolic stress in obesity. Proteomic profiling reveals early biomarkers of therapeutic efficacy, and future research should examine EC’s time-dependent effects on ribosomal biogenesis and metabolic regulation. Supplementary Material File (sousa-filho et al 2025 proteomic.docx) Download 591.68 KB Information & Authors Information Version history V1 Version 1 14 April 2025 Peer review timeline Published PROTEOMICS – Clinical Applications Version of Record 17 Oct 2025 Published Copyright This work is licensed under a Non Exclusive No Reuse License. Collection Clinical Applications Keywords epicatechin obesity polyphenols proteome Authors Affiliations Celso Pereira Batista Sousa-Filho Cruzeiro do Sul University - Analia Franco Campus View all articles by this author Allanis Ferreira Valon Cruzeiro do Sul University - Analia Franco Campus View all articles by this author Talita Souza Siqueira University of São Paulo View all articles by this author Leo Iwai 0000-0002-1571-7763 Instituto Butantan View all articles by this author Alison Felipe Alencar Chaves Instituto Butantan View all articles by this author Victoria Silva 0000-0002-6253-0387 Cruzeiro do Sul University - Analia Franco Campus View all articles by this author Guilherme Ribeiro Romualdo São Paulo State University (UNESP) View all articles by this author Rosemari Otton 0000-0001-5503-3967 [email protected] Cruzeiro do Sul University - Analia Franco Campus View all articles by this author Metrics & Citations Metrics Article Usage 362 views 159 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Celso Pereira Batista Sousa-Filho, Allanis Ferreira Valon, Talita Souza Siqueira, et al. Impact of epicatechin supplementation on plasma proteome profiles in obese men and women -- an exploratory approach to sexual dimorphism. Authorea . 14 April 2025. DOI: https://doi.org/10.22541/au.174461959.94166946/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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