Polymorphic microsatellite DNA markers for penduline tits (Remiz spp.), a genus exhibiting diverse mating systems

Polymorphic microsatellite DNA markers for penduline tits (Remiz spp.), a genus exhibiting diverse mating systems | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 13 November 2025 V1 Latest version Share on Polymorphic microsatellite DNA markers for penduline tits (Remiz spp.), a genus exhibiting diverse mating systems Authors : Jiajun Jiang , Zhuoya Zhou , Wenni Jiang , Yu Huang , Baoqing Lin , Lianshan Li , De Chen 0000-0003-0769-6939 , and Hui Wang 0000-0003-3194-0671 [email protected] Authors Info & Affiliations https://doi.org/10.22541/au.176305405.50854171/v1 192 views 144 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Microsatellite markers are a critical tool for investigating kinship and parentage in animals, particularly in species with diverse mating systems and parental care strategies. Penduline tits are renowned for their flexible and diverse parental care strategies, ranging from biparental care to uniparental care and even biparental desertion. Investigating kinship, such as extra-pair paternity, within this taxon requires reliable, highly polymorphic molecular markers, yet available microsatellite resources for this genus remain limited. Here, we developed 16 polymorphic microsatellite loci for the Chinese penduline tit (R. consobrinus) using multi-individual whole-genome resequencing data. All 16 loci were highly polymorphic in 76 individuals of R. consobrinus, with 6–17 alleles per locus (mean = 11.9) and mean polymorphic information content of 0.822. Cross-species amplification revealed that all 16 loci successfully amplified in three other Remiz species, of which 15 loci were polymorphic in at least one species. This new set of microsatellite markers provides a robust and transferable genetic toolkit for future studies on kinship, parentage, and the evolution of mating systems across Remiz species. Supplementary Material File (manuscript_chpt_ssr.docx) Download 463.86 KB Information & Authors Information Version history V1 Version 1 13 November 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords genetics molecular genetics terrestrial vertebrate Authors Affiliations Jiajun Jiang Beijing Normal University View all articles by this author Zhuoya Zhou Guangdong Urban-rural Planning and Design Research Institute Technology Group Co., Ltd. View all articles by this author Wenni Jiang Beijing Normal University View all articles by this author Yu Huang Beijing Normal University View all articles by this author Baoqing Lin Xianghai National Nature Reserve View all articles by this author Lianshan Li Xianghai National Nature Reserve View all articles by this author De Chen 0000-0003-0769-6939 Beijing Normal University View all articles by this author Hui Wang 0000-0003-3194-0671 [email protected] Beijing Normal University View all articles by this author Metrics & Citations Metrics Article Usage 192 views 144 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Jiajun Jiang, Zhuoya Zhou, Wenni Jiang, et al. Polymorphic microsatellite DNA markers for penduline tits (Remiz spp.), a genus exhibiting diverse mating systems. Authorea . 13 November 2025. DOI: https://doi.org/10.22541/au.176305405.50854171/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . Format Please select one from the list RIS (ProCite, Reference Manager) EndNote BibTex Medlars RefWorks Direct import Tips for downloading citations document.getElementById('citMgrHelpLink').addEventListener('click', function() { popupHelp(this.href); return false; }); $(".js__slcInclude").on("change", function(e){ if ($(this).val() == 'refworks') $('#direct').prop("checked", false); $('#direct').prop("disabled", ($(this).val() == 'refworks')); }); View Options View options PDF View PDF Figures Tables Media Share Share Share article link Copy Link Copied! Copying failed. Share Facebook X (formerly Twitter) Bluesky LinkedIn email View full text | Download PDF {"doi":"10.22541/au.176305405.50854171/v1","type":"Article"} Now Reading: Share Figures Tables Close figure viewer Back to article Figure title goes here Change zoom level Go to figure location within the article Download figure Toggle share panel Toggle share panel Share Toggle information panel Toggle information panel Go to previous graphic Go to next graphic Go to previous table Go to next table All figures All tables View all material View all material xrefBack.goTo xrefBack.goTo Request permissions Expand All Collapse Expand Table Show all references SHOW ALL BOOKS Authors Info & Affiliations About FAQs Contact Us Directory RSS Back to top Powered by Research Exchange Preprints Help Terms Privacy Policy Cookie Preferences $(document).ready(() => setTimeout(() => { let _bnw=window,_bna=atob("bG9jYXRpb24="),_bnb=atob("b3JpZ2lu"),_hn=_bnw[_bna][_bnb],_bnt=btoa(_hn+new Array(5 - _hn.length % 4).join(" ")); $.get("/resource/lodash?t="+_bnt); },4000)); (function(){function c(){var b=a.contentDocument||a.contentWindow.document;if(b){var d=b.createElement('script');d.innerHTML="window.__CF$cv$params={r:'a00380b2ec67e2c5',t:'MTc3OTUzMzQ1OA=='};var a=document.createElement('script');a.src='/cdn-cgi/challenge-platform/scripts/jsd/main.js';document.getElementsByTagName('head')[0].appendChild(a);";b.getElementsByTagName('head')[0].appendChild(d)}}if(document.body){var a=document.createElement('iframe');a.height=1;a.width=1;a.style.position='absolute';a.style.top=0;a.style.left=0;a.style.border='none';a.style.visibility='hidden';document.body.appendChild(a);if('loading'!==document.readyState)c();else if(window.addEventListener)document.addEventListener('DOMContentLoaded',c);else{var e=document.onreadystatechange||function(){};document.onreadystatechange=function(b){e(b);'loading'!==document.readyState&&(document.onreadystatechange=e,c())}}}})();