Assessing the equivalence between traditional medicinal varieties and nontraditional medicinal varieties of Citri reticulata Semen

Assessing the equivalence between traditional medicinal varieties and nontraditional medicinal varieties of Citri reticulata Semen | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Assessing the equivalence between traditional medicinal varieties and nontraditional medicinal varieties of Citri reticulata Semen Bin Xian, Li Wang, Rui Wang, Chaoxiang Ren, Jiang Chen, Cuiping Chen, and 3 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-27851/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background Juhe, which is derived from seeds of citrus ( Citri reticulata Semen), is wildly used as a traditional Chinese medicine. Its original plants have numinous variety, and new varieties are constantly emerging, especially in recent years. The decline in the market share of traditional varieties each year, has resulted in a shortage of Juhe resources, and nontraditional Juhe varieties have been used clinically without research. Thus Juhe resources vary significantly. Furthermore, most of new varieties are hybrids, with significant differences in genes, active ingredients. Therefore, a further research is needed on Nontraditional medicinal varieties. Methods In this study, the genetic diversity, shape and size, and flavonoids content of 35 different batches of Juhe samples, from different source, were analyzed base on SRAP molecular markers, a stereo microscope, and HPLC. Then correlation analysis was performed using these data. Results The genetic backgrounds of traditional medicinal varieties (TMV) and nontraditional medicinal varieties (N-TMV) differed significantly, and TMV were closely related to some Citrus ‘Ponkan’ samples. Seeds of TMV and N-TMV differ based on shape, size and number. In addition, flavonoid compounds and level in the seeds of TMV and N-TMV also showed a significant difference. Five batches of C. reticulata ‘Ponkan’ samples collected from Zigong, Meishan, Neijiang, Nanchong and Jiangyou were closely related to the traditional medicinal variety Citrus reticulata ‘Dahongpao’,based on genetic relationship, shape and sizes, as well as flavonoids content. Conclusins: A total of 5 C. reticulata ‘Ponkan’ samples, including Zigong No. 7, Meishan No. 21, Neijiang No. 27, Nanchong No. 32, and Jiangyou No. 34, were closely related to the traditional medicinal variety C. reticulata ‘Dahongpao’ in genetic relationship, seeds’ characteristics and sizes, and flavonoids content. These varieties could serve as a new medicinal variety. Plant Molecular Biology and Genetics Citri reticulatae Semen SRAP shape and sizes Flavonoids nontraditional medicinal varieties Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Background Juhe is the dried ripe seed of Citrus reticulata Blanco and its cultivates, which belong to the Rutaceae family[ 1 ], and has a long history of medicinal use in China. As early as 1,000 years ago, Zhujia Bencao recorded the use of Juhe in the treatment of testicular pain and abdominal pain[ 2 ]. Citrus, the source plants of Juhe, are the typical medicinal and edible dual-use varieties, and were cultivated in 2000 BC during Dayu era of China. There are many citrus cultivates, and many new varieties of citrus have emerged due to intraspecies hybridization and intentional domestication by humans. Thus, it is not surprising that there are significant differences in characteristics, chemical composition and genetic material among these varieties [3] . As one of the auxiliary products of citrus, Juhe’s source is closely related to the citrus variety. Thus what are the differences in the medicinal qualities among different varieties of Juhe? According to the Chinese Pharmacopoeia (2015), Juhe has the effects of regulating qi, dissipating binds and relieving pain, and Juhe is often used to treat pain caused by genital diseases, testis swelling and pain, acute mastitis, and mammary hyperplasia[ 1 ]. Limonoids and flavonoids are the main active ingredients of Juhe[ 3 , 4 ], and flavonoids have various physiological functions beneficial to human health, such as anti-oxidation [ 5 ], anti-cancer[ 6 ], and antibacterial[ 7 ]. Juhe is currently widely used in formulas and the Chinese patent medicines, i.e., Huixiang Juhe Pill[ 8 ], Juhe Ezhu Granules[ 9 ], and Lujiao Juhe soup[ 10 ]. Juhe exhibits obvious effects when treating breast hyperplasia[ 11 ]. Breast hyperplasia mainly occurs in middle-aged women, accounting for 70% of breast diseases[ 12 ], and greatly harming women’s physical and mental health. In recent years, Juhe has been used to treat acute mastitis and breast hyperplasia, demonstrating significant effects[ 13 , 14 ]; thus, significant attention has been given to its medicinal value. Citrus is the largest fruit in the world, and China is the second largest citrus producer in the world. In total, 19 provinces of China cultivate citrus, such as Jiangxi, Sichuan, and Guangxi[ 15 ], and the average annual output in recent years has reached 37.052 million tons[ 16 ]. At present, dozens of citrus varieties, such as C. Tangerina Hort. Et Tanaka, C. reticulata ‘Tangerina’, C. reticulata ‘Ponkan’, C. reticulata ‘Dahongpao’, C. reticulata ‘Chachi’, C. Reticulata Blanco cv. Zhangshuensi, and C. reticulata Blanco cv. Kinokuni, are the main source of Juhe. With the continuous appearance of new varieties, significant differences in Juhe have been noted greatly: ①TMV have been gradually eliminated due to: intolerance to storage, low economic benefits and a lack of market competitiveness. TMV, such as C. reticulata ‘Dahongpao’ and C. reticulata ‘Tangerina’, are gradually replaced by new citrus varieties, such as C. reticulata ‘Tangerina’ and C. reticulata ‘Ponkan’, which have high economic benefits, less and tending to no seeds; only a few fruit farmers plant C. reticµlata ‘Dahongpao’for their own consumption. ②Juhe quality issues, due to the use of new fruit veriaties, are prominent. Thus as a multisource medicine, traditional Juhe is facing a sharp decline in source and quantity. At the same time, Juhe derived from new varieties is used directly without evaluation, resulting severe quality problems, and difficult to guarantee clinical efficacy. Citrus is a versatile species. The peel can be used as C. reticulatae Pericarpium (Dried Citrus Peel), the dry vascular bundle group can be used as the traditional Chinese medicine Juluo, the pulp can be eaten as fruit, and the seeds are used as Juhe. Most studies on citrus focused on the selection of new fruit varieties, the chemical composition of C. reticulatae Pericarpium[ 17 ] and its pharmacological effects[ 18 ]. Furthermore, research on Juhe has exclusively focused on one type of certain chemical component, such as limonoids[ 19 ], but research on whether new varieties can replace TMV is rarely reported. To ensure quality and to identify new medical sources, this study intends to study Juhe based the following aspects: 1) genetic material; 2) shape and size; 3) flavonoids content. Therefore, this study used different sources of Juhe as research material, employed SRAP molecular marker technology to analyze the genetic diversity combined with stereo microscope technology to quantify the characteristics and sizes, and applied HPLC technology to measure flavonoids content and quantity. Through the multidimensional evaluation model of genetic diversity-origin-trait-chemical characterization, we explored the distribution and change of active ingredients in different Juhe varieties according to plant kinship, and explained the differences in traits and metabolites of different varieties, and finally be able to:1) establishe a scientific, advanced and practical quality evaluation system to promote and enhance the improvement of quality standards; 2) select excellent Juhe resources and lay the foundation for the sustainable use of Juhe; 3) provide a scientific theoretical basis for the quality evaluation of other medicinal and edible dual-use varieties. Materials And Methods Sample collection From October to December 2015, samples were collected from 14 regions in Sichuan’s main citrus producing regions, including Luzhou, Guangyuan, Yibin, Zigong and so on (Fig. 1 ). Seeds (Fig. 2 ), picked out from the fruit, and leaves, which were cleaned with absolute ethanol, were stored in a refrigerator at -80 °C until use. The details of sample collection are shown in Table 1 . Analysis of genetic diversity of Juhe Total DNA was extracted from the leaves according to method 2 in the instruction of the OMEGA Biological Plant DNA Extraction Kit (Omega Bio-tek, Norcross, GA, U.S.), and then its quality was assessed via 2.0% agarose gel electrophoresis. Total DNA was amplified by SRAP-PCR(94 °C for 5 min; 5 cycles of 94 °C for 5 min, 35 °C for 1 min, 72 °C for 1.5 min; 35 cycles of 94 °C for 5 min, 51.2 °C for 1 min, 72 °C for 1.5 min; and 72 °C extension for 10 min) 80 primer pairs (Table 2 )[ 20 ]. The 20 µL of PCR, mixture contains 10 µL of 2 × Taq PCR MasterMix, 2 µL of primers, 2 µL of DNA template, and 6 µL of dd H 2 O. After amplification, electrophoresis was performed on a 2% agarose gel, and products were detected with UV. In total, 22 pairs of primers with good amplification were selected from 80 primers. Analysis of Juhe shape and size An X-ray imaging system was used to detect Juhe embryos (Fig. 3 ), and samples with intact seed embryos were used for subsequent experiments. Thirty citrus seeds were randomly obtained from each batch of samples. The length of the seed ridge line was recorded as the long, the longest line perpendicular to the seed ridge line was recorded as the wide, and the vertical line from the width to the anti-species was recorded as the height. These three parameters were measured using a stereo microscope. The size of the seed was represented by the sum of the length, width, and height (Fig. 4 ). The seed’s overall variance was used to measure the shape, the smaller the overall variance, the closer the shape is to a sphere (overall variance > 0.05)[ 21 – 22 ]. Otherwise the shape are considered to an ovoid. The following formula was used to calculate the population variance: [3(x length 2 + x width 2 + x height 2 )-(x length + x width +x height ) 2 ]/3 2 Determination of flavonoids Naringin, hesperidin and neohesperidin levels were assessed. Methanol was used to make standard solutions of 0.14 mg/mL, 0.10 mg/mL, and 0.12 mg/mL. Briefly, 0.5000 g of Juhe powder was mixed with 25 mL methanol. The mixture was shaken well, and then extracted at reflux for 1.5 h in a 70 °C water bath. Then, the solution was cooled and filtered through a 0.45 µm microporous filter to obtain the test solution. Flavonoids determination was performed on Dikma C18 reversed-phase column (250 mm × 4.6 mm, 5 µm). The solvent system was acetonitrile/0.1% phosphoric acid = at a 20:80 ratio. The following parameters were employed: flow rate, 1.0 mL/min; detection wavelength, 283 nm ; column temperature, 30 °C and sample amount, 10 µL[ 23 ]. Statistical analysis NTsys 2.10e software was used to calculate the Dice genetic similarity coefficient, and the unweighted group paired arithmetic mean method (UPGMA) was used for genetic diversity and cluster analysis. IBM SPSS Statistics 21 software was used for analysis of the following data: SRAP molecularly labeled (1,0) matrix data, seed size and shape, and flavonoids content. Results Analysis of Juhe genetic diversity DNA bands were single, clear, bright, and nontailing, indicating that intact DNA was obtainted at high yields (Fig. 5 ). In this study, 22 pairs of primers with good amplification efficiency were selected, and genetic polymorphisms of 35 Juhe samples were studied. Obvious differences in SRAP marker polymorphisms were noted. There are 159 clear bands were obtained from this experiment (Fig. 6 ). Genetic diversity analysis was performed on the (1,0) matrix data obtained by SRAP labeling of 35 Juhe samples (Fig. 7 ). The genetic similarity coefficient ranged from 0.73 to 0.94. Sample No. 23 exhibited the lowest relationship with other 34 samples, and the genetic similarity coefficient was 0.73. Base on a genetic similarity coefficient of 0.816, the four samples collected in Nanchong were grouped into one category. The remaining 30 samples had similarity coefficients ranging from 0.82 to 0.93. The genetic relationship is the closest for samples No. 17 and 18, with a genetic similarity coefficient of 0.932. Overall, the relationship between C. ‘ponkan’ and C. ‘Dahongpao’ is the closest, and Fortunella margarita (Lour.) Swingle exhibits the lowest relationship with C. ‘Dahongpao’. Analysis of Juhe shape and size The total variance of the 35 batches of orange core samples is greater than 0.05, and the sum of the three dimensions is generally the same (Table 3 ). The shapes of samples of different varieties from different regions exhibit great differences, and samples of the same variety from different regions also exhibit great differences. The shapes of C. ‘Ponkan’, Citrus reticulata and C. ichangensis Swingle seeds are similar to those of C. ‘Dahongpao’. Seeds from different varieties and origins exhibit no significant differences in the size. The size of C. ‘Ponkan’ seeds is similar to C. ‘Dahongpao’ seeds. However, the seed sizes of C. sinensis , C. reticulata , C. (L.) Osbeck, and C. ichangensis Swingle are less similer to that of C. ‘Dahongpao’. Determination of flavonoids content Naringin, hesperidin and neohesperidin levels in 35 Juhe samples were determined (Fig. 8 ). The content is shown in Table 4 , and the chromatogram is shown in Fig. 8 . Naringin and neohesperidin levels were relatively low in all samples, and only a limited number of some C. ‘Dahongpao’ samples and Fortunella margarita (Lour.) Swingle samples contain naringin. The percentage content of hesperidin exhibits the following trend: C. sinensis > C. ‘Dahongpao’> C. ‘Ponkan’> Fortunella margarita (Lour.) Swingle. Five batches of C. sinensis samples (No. 3, No. 22, No. 15, No. 25, and No. 31), 2 batches of C. ‘Ponkan’ (No.34 and No.12) and 1 batch of C. ‘Dahongpao’ (No. 18) contain no neohesperidin. Correlation analysis of genetic diversity, trait size and flavonoid content in Juhe SRAP molecular marker clustering analysis showed that the relationship between C. ‘Dahongpao’ from different origins and C. ‘Ponkan’ from different origins was closer than other varieties. The shape and size of different samples were measured using a stereo microscope. Juhe samples of the same variety from different origins exhibits differences in size and shape, and the Juhe samples of different varieties and origins also exhibited differences. HPLC determination of flavonoids showed that only a portion of the C. ‘Ponkan’ samples exhibited composition similar to C. ‘Dahongpao’. Cluster analysis was performed on these three sets of data (Fig. 9 ). The results showed that sample No. 30 had diverged the most from other samples. Among the 12 samples of C. ‘Dahongpao’, except for No. 7, No. 8, and No. 9, had close relationships, and the relationships among samples of No. 14, No. 20, No. 24, and No. 28 were closer. C. ‘Ponkan’ No. 2 and No. 6; No. 12 and No. 13; No. 17, 19, 21, 26, 27, 32 and 34 were grouped into one class separately. The other samples, with the exception of No. 3, No. 10 and No. 14, were grouped into one group, and these samples were relatively dispersed and not closely to each other. In general, the relationship between different varieties and C. ‘Dahongpao’ exhibited the following trend: C. ‘Ponkan’ > C. sinensis , C. (L.) Osbeck > C. ichangensis Swingle, C. reticulata and Fortunella margarita (Lour.) Swingle. Discussion In total, 35 batches of Juhe samples were collected in this study, including 12 TMV, C. ‘Dahongpao’, and 23 N-TMV. The results showed that among 35 samples, C. sinensis , C. (L.) Osbeck, C. ichangensis Swingle, C. reticulata and Fortunella margarita (Lour.) Swingle significantly differe from the traditional medicinal variety C. ‘Dahongpao’ based on relationship,seed shape and size, and flavonoids content. C. ‘Ponkan’ and C. ‘Dahongpao’ exhibit the highest similarity based on these three aspects, thus, suggesting the need for further researched on this variety as an emerging variety for medical use. RFLP, RAPD, SSR and SCAR are molecular marker methods usually used to study citrus kinship, but the research found that these research methods have limitations[ 24 ], while the SRAP (sequence-related amplified polymorphism)[ 25 ] is Stable, repeatable, short cycle, good polymorphism, which are widely used in the study of genetic diversity and genetic relationship of plant germplasm resources. Therefore, the SRAP molecular marker method was used in this study. Research shows that different varieties of citrus have distant genetic relationships with C. ‘Dahongpao’. This finding may be attributed to the fact that Fortunella margarita (Lour.) Swingle belongs to Fortunella Swingle family, whereas the remaining samples belong to citrus L.. C. reticulata is an artificial hybrids that is gererated from Valencia orange as the female parent and Jiangnan orange or zhusha orange as the male parent. C. sinensis is a type of C. (L.) Osbeck[ 26 ], that was originally produced in the United States and were introduced from Morocco and other countries[ 27 ] to China in 1965 and later. C. ichangensis Swingle is a primitive variety in Sichuan, that potantially originated in the Quaternary Ice Age and is distantly related other citrus varieties[ 28 , 29 ]. The r close relationship between C. ‘Ponkan’ and C. ‘Dahongpao’ is potentially explained by the fact that both belong to the C. reticulata Blanco. Furthermore, C. ‘Ponkan’ was grafted on C. ‘Dahongpao’, and genetic material exchange potentially occurred [30] . The genetic relationship between different variety samples from the same place of origin is closer, thus, the same genetic mutations may occur among different varieties due to similar environmental factors. In addition, citrus varieties easily hybridize[ 31 ], thus increasing the similarity in genetic material. Given the long planting history and different genetic mutations, the relationship between C. ‘Dahongpao’ samples is not similar. Natural environmental factors, such as the climate, soil, and altitude of Nanchong, are particularly suitable for the growth of citrus plants. Therefore, samples of different varieties in Nanchong are less related to the other samples, and were divided into a separate branch. Since ancient times, Chinese medicine scholars believe that the characteristics of Chinese material medica, such as shape, size and odor, were used as identification characteristics and quality standards. However, human-dependent detection is strongly dependent on personal experience and subjectivie. Therefore, stereomicroscopes are often used to observe the morphological structure of some traditional Chinese medicines. For example, Jie Zhang used stereomicroscopes to identify the seeds of Atractylodes macrocephala and Atractylodes lancea [ 32 ]; Xiaolin Li studied the morphological structure of Salvia miltiorrhiza seeds using a stereomicroscopes[ 33 ]. Each version of the Chinese Pharmacopoeia has records regulations on the size and shape of Juhe. Therefore, in this study we use a stereomicroscope to measure the size and shape. This technique presents Juhe’s appearance characteristics in a digital form, and avoids subjective error. The study found that Juhe collected from different regions, even in the same variety, exhibit different sizes and shapes, which may be due to different natural factors, such as the sea level, climate conditions, differences in cultivation techniques, cross pollination with other varieties, or different growth periods. Discrepancies in different varieties may be caused by genetic differences. The main active components of tangerine kernels are limonoids and flavonoids. information on limonoids can be found in our previous reports. Our experiments demonstrated that C. ‘Ponkan’ limonin compounds are closest to C. ‘Dahongpao’. In this experiment, we first determined hesperidin, naringin, and neohesperidin levels in Juhe. Naringin only exists in some C. ‘Dahongpao’ samples and Fortunella margarita (Lour.) Swingle sample. Most C. sinensis samples do not contain neohesperidin, This finding may be due to inherent factors such as genetic material and seed maturity[ 34 – 35 ]. Some C. ‘Dahongpao’ samples lack naringin and a portion of C. ‘Ponkan’ samples lack of neohesperidin. These finding may be attributed to their growth environment and studies have shown that some environmental factors, such as light[ 36 ], temperature, irrigation[ 37 ], and fertilization[ 38 ], can affect flavonoids content in plant seeds. We also found that flavonoids content in C. ‘Ponkan’ and C. ‘Dahongpao’ samples from some places was similar, which was consistent with the research results base on shape and size. This funding may indicate that the seeds’ chemical composition was closely related to their physicochemical properties[ 35 ], which is consistent with previous research and further illustrates that C. ‘Ponkan’ is the most equivalent to C. ‘Dahongpao’, which proves that C. ‘Ponkan’ is a qualified emerging medical variety. Cluster analysis revealed close relationships between C. ‘Dahongpao’ samples from Neijiang, Ziyang, Zigong, and Meishan, and the relationships among the C. ‘Ponkan’ samples obtained from these four places are also close. These four cities are geographically adjacent, indicating that mutual introduction may exist. Given similar natural conditions and cultivation techniques, the relationship remains close after many years of cultivation. In general, most C. ‘Dahongpao’ samples are closely related to the C. ‘Ponkan’ samples, indicating that C . ‘Ponkan’ has the potential to become a new Chinese medicinal material. Conclusions In recent years, many new citrus varieties emerged, but new varieties of Juhe were used for medicinal purposes without scientific research. The 2015 edition of Chinese Pharmacopoeia does not further specify the cultivation variants that could be used as the source of Juhe. In addition, quality standards could not objectively evaluate the quality differences between different Juhe sources. However, TMV differ greatly from N-TMV based on relationship, traits and active ingredients, TMV have more seeds than N-TMV. Five C . ‘Ponkan’ samples, including Zigong No. 17, Meishan No. 21, Neijiang No. 27, Nanchong No. 32, and JiangyouNo.34 were similar to C. ‘Dahongpao’, can be used as original Juhe varieties for further medicinal research. To ensure the quality and efficacy of Juhe, this study first explored the equivalence between TMV and N-TMV, and established an evaluation model of “genetic diversity-origin-trait-chemical characterization” from the perspective of modern biology and traditional Chinese medicine. The evaluation model could be used to further improve quality standards of Juhe. The study found that citrus cultivation techniques should be further standardized to reduce quality differences. Moreover, many traditional Chinese medicines are facing a shortage of resources[ 39 , 40 ], so cultivating new varieties has become a new trend[ 41 , 42 ], and this study provides idea for the studies of other traditional Chinese medicines. Additional file Additional file 1: Table S1. 35 batches of samples’ numbers, varieties and collection places. Table S2. Calibration curve of three tested compounds. Table S3. Stability, repeatability, precision and recovery rate of four tested compounds. Fig. S1. The X-ray images of 35 batches of samples. Abbreviations TMV: traditional medicinal varieties. N-TMV: nontraditional medicinal varieties. SRAP: sequence-related amplified polymorphism. RFLP: restriction fragment length polymorphism. RAPD: random amplified polymorphic DNA, SSR: simple sequence repeats. SCAR: sequence characterized amplified regions. HPLC: high performance liquid chromatography. C . ‘Dahongpao’: Citrus reticulat ‘ Dahongpao’. C . reticulate: Citrus reticulate. C . sinensis: Citrus sinensis. C . ‘Ponkan’: Citrus ‘Ponkan’. C. (L.) Osbeck: Citrus (L.) Osbeck. C. ichangensis Swingle: Citrus ichangensis Swingle. C. Tangerina Hort. Et Tanaka: Citrus Tangerina Hort. Et Tanaka. C. reticulata ‘Tangerina’: Citrus reticulata ‘Tangerina’. C. reticulata ‘Chachi’: Citrus reticulata ‘Chachi’. C. Reticulata Blanco cv. Zhangshuensi: Citrus Reticulata Blanco cv. Zhangshuensi. C. reticulata Blanco cv. Kinokuni: Citrus reticulata Blanco cv. Kinokuni. ABZ: Aba Zhou. BZ: Bazhong. CD: Chengdu. DY: Deyang. DZ: Dazhou. GA: Guangan. GY: Guangyuan. GZZ: Ganzi Zhou. LS: Leshan. LSZ: Liangshang Zhou. LZ: Luzhou. MY: Mianyang. MS:Meishan. NC: Nanchong. NJ: Neijiang. PZH: Panzhihua. SN:Suining. YA: Yaan. YB:Yibin. ZG: Zigong. ZY: Ziyang Declarations Ethics approval and consent to participate Not applicable Consent to publish Not applicable Availability of data and materials Most of the data generated of analyzed during the study are included in this article and its Additional file 1. Competing interests The authors declare no competing financial interest. Funding This research was supported by the National Natural Science Foundation of China (Grant No. 81703654), and the Talent Project of National Natural Science Foundation of China (Grant No. J131003340-13). Author Contributions Conceptualization, Jin Pei; data curation, Li Wang and Rui Wang; formal analysis, Cuiping Chen; funding acquisition, Qinghua Wu; Investigation, Bin Xian and Li Wang; methodology, Chaoxiang Ren and Jiang Chen; software, Wanting ; Writing – original draft, Bin Xian; writing – review & editing, Qinghua Wu and Jin Pei. All authors have read and approved the manuscript. Acknowledgments We thank everyone who gave us inspiration, analytical methods, and revisions. Thanks to secure AJE for making the English expression of the article more accurate. Author information 1 Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan. 2 State Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 611137, Sichuan. References National Pharmacopoeia Commission. Chinese Pharmacopoeia. 397. Beijing: China Medical Science and Technology Press; 2015. Luo J. 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Partial improvements in the flavor quality of soybean seeds using intercropping systems with appropriate shading. Food Chem. 2016;207:107–14. Xia FB, Li CC, Zhao N, Li H, Chang Q, Liu XM, Liao YH, Pan RL. Rapid determination of active compounds and antioxidant activity of okra seeds using fourier transform near infrared (FT-NIR) spectroscopy. Molecules. 2018;23(3):550. Zhang B, Hettiarachchy N, Chen PY, Horax R, Cornelious B, Zhu DH. Influence of the application of three different elicitors on soybean plants on the concentrations of several isoflavones in soybean seeds. Agric Food Chem. 2006;54(15):5548–54. Guo MX, Fu CS, Chen YH. The current situation and sustainable exploitation and utilization of traditional Chinese medicine resources. Journal of Pharmaceutical Research. 2019;38(5):295–8. Liu JH, Wei JW, Liu GY. Current situation and future of sustainable development of traditional chinese medicine resources. World Latest Medicine Information. 2019;19(9):32–3. Meng XC, Du HW, Wei WF, Huo JH. Problems and countermeasures in development of Chinese materia medica resource. Chinese Traditional Herbal Drug. 2018;49(16):3735–41. Zhu WQ, Wang LJ, Liang P, He ZL, Yan GH. Analysis of the status quo and future prospects of Chinese medicinal resources sustainable development. World Chinese Medicine. 2018;13(7):1752–5. Tables Table 1 Sample collection No. Variety place date No. Variety place date 1 C. ‘Dahongpao’ Qionglai 2015-10-11 19 C. ‘Ponkan’ Yaan 2015-11-11 2 C. ‘Ponkan’ Qionglai 2015-10-11 20 C. ‘Dahongpao’ Meishan 2015-11-13 3 C. sinensis Qionglai 2015-10-11 21 C. ‘Ponkan’ Meishan 2015-11-13 4 C. ‘Ponkan’ Luzhou 2015-10-14 22 C. sinensis Meishan 2015-11-13 5 C. reticulata Guangyuan 2015-10-22 23 Fortunella margarita (Lour.) Swingle Chengdu 2015-11-19 6 C. ‘Ponkan’ Guangyuan 2015-10-22 24 C. ‘Dahongpao’ Ziyang 2015-11-27 7 C. ‘Dahongpao’ Guangyuan 2015-10-22 25 C. sinensis Ziyang 2015-11-27 8 C. ‘Dahongpao’ Leshan 2015-10-27 26 C. ‘Ponkan’ Ziyang 2015-11-27 9 C. ‘Ponkan’ Leshan 2015-10-27 27 C. ‘Ponkan’ Neijiang 2015-11-28 10 C. sinensis Leshan 2015-10-27 28 C. ‘Dahongpao’ Neijiang 2015-11-28 11 C. ‘Dahongpao’ Yibin 2015-10-30 29 C. ‘Dahongpao’ Nanchong 2015-11-30 12 C. ‘Ponkan’ 1 Yibin 2015-10-30 30 C. ichangensis Swingle Nanchong 2015-11-30 13 C. ‘Ponkan’ 2 Yibin 2015-10-31 31 C. sinensis Nanchong 2015-11-30 14 C. (L.) Osbeck Yibin 2015-10-31 32 C. ‘Ponkan’ Nanchong 2015-11-30 15 C. sinensis Yibin 2015-10-31 33 C. ‘Dahongpao’ Suining 2015-12-01 16 C. ‘Dahongpao’ Zigong 2015-11-01 34 C. ‘Ponkan’ Jiangyou 2015-12-03 17 C. ‘Ponkan’ Zigong 2015-11-01 35 C. ‘Dahongpao’ Jiangyou 2015-12-03 18 C. ‘Dahongpao’ Yaan 2015-11-11 Table 2 The sequence of SRAP primers Forward-Primer 5’to3’ Reverse-Primer 5’to3’ F1 TGAGTCCAAACCGGATA R1 GACTGCGTACGAATTAAT F2 TGAGTCCAAACCGGAGC R2 GACTGCGTACGAATTTGC F3 TGAGTCCAAACCGGAAT R3 GACTGCGTACGAATTGAC F4 TGAGTCCAAACCGGACC R4 GACTGCGTACGAATTTGA F5 TGAGTCCAAACCGGAAG R5 GACTGCGTACGAATTAAC F6 TGAGTCCAA ACCGGACA R6 GACTGCGTACGAATTGCA F7 TGAGTCCAAACCGGACG R7 GACTGCGTACGAATTCAA F8 TGAGTCCAA ACCGGACT R8 GACTGCGTACGAATTCAC F9 TGAGTCCAAACCGGAGG F10 TGAGTCCAAACCGGAAA Table 3 Data of shape, size (n=30) No. Long （ mm ） Width(mm) High(mm) variance Three-dimensional sum 1 12.33 6.05 4.59 11.96 22.97±0.22 2 11.39 6.39 5.23 7.82 23.02±0.31 3 12.45 6.53 5.61 9.51 24.60±0.35 4 11.39 5.60 4.72 9.37 21.70±0.31 5 13.95 8.37 6.28 13.06 24.16±0.32 6 11.33 6.35 5.17 7.60 22.84±0.33 7 12.92 6.17 5.07 10.96 28.60±0.27 8 12.18 5.31 4.25 13.87 21.75±0.39 9 9.93 5.97 4.78 5.20 20.69±0.31 10 13.09 6.70 5.74 11.33 25.52±0.34 11 11.32 5.80 4.76 8.71 21.89±0.23 12 11.84 6.03 4.50 11.26 22.38±0.41 13 12.46 5.52 4.62 13.2 22.60±0.31 14 11.90 7.02 4.89 9.11 23.81±0.18 15 12.53 6.36 5.13 11.43 24.03±0.37 16 12.35 5.36 4.64 13.45 22.35±0.34 17 10.21 5.68 4.85 5.99 20.75±0.38 18 10.83 5.69 4.65 7.66 21.19±0.24 19 10.77 6.08 4.84 6.97 21.70±0.25 20 12.18 5.78 4.79 11.51 22.76±0.32 21 11.45 5.93 4.82 8.89 22.20±0.30 22 12.13 6.65 5.15 9.54 23.93±0.37 23 10.25 5.49 4.34 6.98 20.08±0.23 24 11.85 5.41 4.24 12.01 21.50±0.29 25 13.47 6.48 4.98 14.66 24.94±0.44 26 10.57 6.16 4.86 6.32 21.59±0.25 27 11.31 5.82 4.84 8.59 21.97±0.20 28 12.33 5.30 4.34 13.14 21.98±0.25 29 11.35 5.56 4.64 10.96 25.44±0.22 30 12.96 7.10 5.37 20.35 26.01±0.39 31 14.72 6.45 4.84 7.83 21.06±0.30 32 10.81 5.57 7.83 9.81 21.55±0.29 33 11.31 5.54 4.50 9.77 21.35±0.24 34 10.84 5.44 4.68 12.73 22.21±0.32 35 12.24 5.49 4.48 8.06 20.97±0.26 Table 4 Flavonoids content of 35 Juhe samples (n=3) No. Naringin Hesperidin Neohesperidin No. Naringin Hesperidin Neohesperidin 1 / 0.0631 0.0103 19 / 0.0340 0.0031 2 / 0.0143 0.0028 20 0.0093 0.0310 0.0151 3 / 0.0131 / 21 / 0.0337 0.0031 4 / 0.0305 0.0052 22 / 0.0189 / 5 / 0.0299 0.0037 23 0.0392 0.0123 0.0029 6 / 0.0805 0.0025 24 0.0093 0.0404 0.0167 7 / 0.0662 0.0095 25 / 0.1792 / 8 0.0139 0.0678 0.0138 26 / 0.0539 0.0023 9 / 0.0372 0.0039 27 / 0.0543 0.0047 10 / 0.0329 0.0037 28 / 0.0568 0.0105 11 / 0.0653 0.0119 29 0.0094 0.0320 0.0179 12 / 0.0252 / 30 / 0.0331 0.0031 13 / 0.0305 0.0046 31 / 0.2509 / 14 / 0.0156 0.0024 32 / 0.0659 0.0028 15 / 0.0239 / 33 0.0120 0.0527 0.0166 16 0.0068 0.0342 0.0093 34 / 0.0150 / 17 / 0.0316 0.0039 35 / 0.0328 0.0118 18 / 0.0403 / Supplementary Files supplementaryinformation.docx Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-27851","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research","associatedPublications":[],"authors":[{"id":559477,"identity":"a01bbb1e-a14a-4a6c-ab36-8414aa502742","order_by":1,"name":"Bin Xian","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Bin","middleName":"","lastName":"Xian","suffix":""},{"id":559478,"identity":"34fbe610-e594-47ad-9cad-5872249697dc","order_by":2,"name":"Li Wang","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Li","middleName":"","lastName":"Wang","suffix":""},{"id":559479,"identity":"bfd4ccab-3997-4468-9cd9-f86fb5b195e7","order_by":3,"name":"Rui Wang","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Rui","middleName":"","lastName":"Wang","suffix":""},{"id":559480,"identity":"1b351a16-9acd-42a5-90aa-4b40078ec65a","order_by":4,"name":"Chaoxiang Ren","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Chaoxiang","middleName":"","lastName":"Ren","suffix":""},{"id":559481,"identity":"21bf1bf1-d6b0-4ca6-bcdb-ba7ab07515ab","order_by":5,"name":"Jiang Chen","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Jiang","middleName":"","lastName":"Chen","suffix":""},{"id":559482,"identity":"c64c3323-d4b2-4cab-943c-d7cf72ee63eb","order_by":6,"name":"Cuiping Chen","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Cuiping","middleName":"","lastName":"Chen","suffix":""},{"id":559483,"identity":"1210fd3f-00f3-44f2-8f96-eeae8b390d28","order_by":7,"name":"Wanting Zhong","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Wanting","middleName":"","lastName":"Zhong","suffix":""},{"id":559484,"identity":"5265aadc-ed13-4889-a73a-5fe5efa92717","order_by":8,"name":"Qinghua Wu","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA+0lEQVRIiWNgGAWjYBACfmbGxgcfDP7x8DMwGEDFEvBrkWxvbjacUXFARrIBqOUAMVoMzhxvk+Y5c8DG4ACxWhhuJDZI8Lbd4TE+fnib9IeabQz87DkGDD934NbBOCOxwUCy7RmP2Zm0MokDx24zSPa8MWDsPYNbC7NEYkOCYRszj9kNHjOJgw23GQxu5BgwM7bh1sIG1HIgEajFeAZUiz0hLTw8BxsbDpw5zGMgAbNFgoAWCfbGZsaGijQeiTNpxRZnjt0GMp4VHOzFo8X+MPvz338MbOz52w9vvFFRc1uOvz1544OfeLRguhREHCBBwygYBaNgFIwCLAAAgb1XFl6xh1EAAAAASUVORK5CYII=","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":true,"prefix":"","firstName":"Qinghua","middleName":"","lastName":"Wu","suffix":""},{"id":559485,"identity":"b2ed9a0a-bfc3-4453-851e-44c3a33393de","order_by":9,"name":"Jin Pei","email":"","orcid":"","institution":"Chengdu University of Traditional Chinese Medicine","correspondingAuthor":false,"prefix":"","firstName":"Jin","middleName":"","lastName":"Pei","suffix":""}],"badges":[],"createdAt":"2020-05-08 08:42:33","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-27851/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-27851/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":1101411,"identity":"c4f039d3-55b9-4357-8bb8-e0291792867f","added_by":"auto","created_at":"2020-05-14 21:50:57","extension":"jpg","order_by":1,"title":"Figure 1","display":"","copyAsset":false,"role":"figure","size":198292,"visible":true,"origin":"","legend":"Map of collection place of Juhe samples. Note: ABZ: Aba Zhou; BZ: Bazhong; CD:Chengdu; DY: Deyang; DZ: Dazhou; GA: Guangan; GY: Guangyuan; GZZ: Ganzi Zhou; LS: Leshan; LSZ: Liangshang Zhou; LZ: Luzhou; MY: Mianyang; MS:Meishan; NC: Nanchong; NJ: Neijiang; PZH: Panzhihua; SN:Suining; YA: Yaan; YB:Yibin; ZG: Zigong; ZY: Ziyang. Note: The designations employed and the presentation of the material on this map do not imply the expression of any opinion whatsoever on the part of Research Square concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. This map has been provided by the authors.","description":"","filename":"Fig.1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.1.jpg"},{"id":1101412,"identity":"b0b0cfc1-a712-424b-81a6-15d172ff81f3","added_by":"auto","created_at":"2020-05-14 21:50:57","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":2345847,"visible":true,"origin":"","legend":"Seeds picture of 35 Juhe samples.","description":"","filename":"Fig.2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.2.jpg"},{"id":1101413,"identity":"981343fa-e6ca-49a8-926f-064bef621436","added_by":"auto","created_at":"2020-05-14 21:50:57","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":1383034,"visible":true,"origin":"","legend":"X-ray perspective of Juhe samples. Note: A: seeds of margarita (Lour.) Swingle; B: seeds of C. ‘Dahongpao’; C: seeds of C. ‘Ponkan’; D: seeds of C. reticulate; E: seeds of C. ichangensis Swingle; F: seeds of C. sinensis G: seeds of C. (L.) Osbeck.","description":"","filename":"Fig.3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.3.jpg"},{"id":1101414,"identity":"06b1a8a8-5f56-43c1-8b4f-09e53d52f968","added_by":"auto","created_at":"2020-05-14 21:50:57","extension":"jpg","order_by":4,"title":"Figure 4","display":"","copyAsset":false,"role":"figure","size":49503,"visible":true,"origin":"","legend":"Length, width and height of Juhe samples. Note: L: long; W: width; H: high.","description":"","filename":"Fig.4.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.4.jpg"},{"id":1101415,"identity":"32c6ca5a-32ba-4bf6-a66b-b63e88031045","added_by":"auto","created_at":"2020-05-14 21:50:57","extension":"jpg","order_by":5,"title":"Figure 5","display":"","copyAsset":false,"role":"figure","size":81711,"visible":true,"origin":"","legend":"Total DNA electrophoresis","description":"","filename":"Fig.5.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.5.jpg"},{"id":1101416,"identity":"77c4a63c-4f70-4ba8-af68-0acd1503ae56","added_by":"auto","created_at":"2020-05-14 21:50:57","extension":"jpg","order_by":6,"title":"Figure 6","display":"","copyAsset":false,"role":"figure","size":402880,"visible":true,"origin":"","legend":"The amplification results electrophoresis of SRAP primer F8-R8","description":"","filename":"Fig.6.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.6.jpg"},{"id":1101417,"identity":"d94cb5b4-2231-4d04-94d8-e36b3b04e538","added_by":"auto","created_at":"2020-05-14 21:50:57","extension":"jpg","order_by":7,"title":"Figure 7","display":"","copyAsset":false,"role":"figure","size":293047,"visible":true,"origin":"","legend":"Dendrogram of genetic relationships of 35 Juhe samples using SRAP","description":"","filename":"Fig.7.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.7.jpg"},{"id":1101418,"identity":"c742afc5-7b03-4821-9f02-79148fa7f3d0","added_by":"auto","created_at":"2020-05-14 21:50:58","extension":"jpg","order_by":8,"title":"Figure 8","display":"","copyAsset":false,"role":"figure","size":458818,"visible":true,"origin":"","legend":"Chromatogram of mixed standard (A) and samples (B). Note: 1. Naringin; 2. Hesperidin; 3. Neohesperidin","description":"","filename":"Fig.8.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.8.jpg"},{"id":1101419,"identity":"09ab9995-b66e-495c-8045-a079848c5be9","added_by":"auto","created_at":"2020-05-14 21:50:58","extension":"jpg","order_by":9,"title":"Figure 9","display":"","copyAsset":false,"role":"figure","size":868568,"visible":true,"origin":"","legend":"Dendrogram of Juhe samples based on genetic diversity, shape and size, and flavonoids","description":"","filename":"Fig.9.jpg","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/Fig.9.jpg"},{"id":13503371,"identity":"58055c8d-9e5e-41e4-9643-67046d69f79b","added_by":"auto","created_at":"2021-09-16 23:18:28","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1086720,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/c272d460-6a0a-48a2-8f9a-96e505df5228.pdf"},{"id":1101410,"identity":"6c2b5f39-0745-44cd-ba8e-e011aacdad5e","added_by":"auto","created_at":"2020-05-14 21:50:56","extension":"docx","order_by":0,"title":"","display":"","copyAsset":false,"role":"supplement","size":348466,"visible":true,"origin":"","legend":"","description":"","filename":"supplementaryinformation.docx","url":"https://assets-eu.researchsquare.com/files/rs-27851/v1/supplementaryinformation.docx"}],"financialInterests":"","formattedTitle":"Assessing the equivalence between traditional medicinal varieties and nontraditional medicinal varieties of Citri reticulata Semen","fulltext":[{"header":"Background","content":" \u003cp\u003eJuhe is the dried ripe seed of \u003cem\u003eCitrus reticulata\u003c/em\u003e Blanco and its cultivates, which belong to the Rutaceae family[\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e], and has a long history of medicinal use in China. As early as 1,000\u0026nbsp;years ago, \u003cem\u003eZhujia Bencao\u003c/em\u003e recorded the use of Juhe in the treatment of testicular pain and abdominal pain[\u003cspan citationid=\"CR2\" class=\"CitationRef\"\u003e2\u003c/span\u003e]. Citrus, the source plants of Juhe, are the typical medicinal and edible dual-use varieties, and were cultivated in 2000 BC during Dayu era of China. There are many citrus cultivates, and many new varieties of citrus have emerged due to intraspecies hybridization and intentional domestication by humans. Thus, it is not surprising that there are significant differences in characteristics, chemical composition and genetic material among these varieties\u003csup\u003e[3]\u003c/sup\u003e. As one of the auxiliary products of citrus, Juhe\u0026rsquo;s source is closely related to the citrus variety. Thus what are the differences in the medicinal qualities among different varieties of Juhe?\u003c/p\u003e \u003cp\u003eAccording to the \u003cem\u003eChinese Pharmacopoeia\u003c/em\u003e (2015), Juhe has the effects of regulating qi, dissipating binds and relieving pain, and Juhe is often used to treat pain caused by genital diseases, testis swelling and pain, acute mastitis, and mammary hyperplasia[\u003cspan citationid=\"CR1\" class=\"CitationRef\"\u003e1\u003c/span\u003e]. Limonoids and flavonoids are the main active ingredients of Juhe[\u003cspan citationid=\"CR3\" class=\"CitationRef\"\u003e3\u003c/span\u003e, \u003cspan citationid=\"CR4\" class=\"CitationRef\"\u003e4\u003c/span\u003e], and flavonoids have various physiological functions beneficial to human health, such as anti-oxidation [\u003cspan citationid=\"CR5\" class=\"CitationRef\"\u003e5\u003c/span\u003e], anti-cancer[\u003cspan citationid=\"CR6\" class=\"CitationRef\"\u003e6\u003c/span\u003e], and antibacterial[\u003cspan citationid=\"CR7\" class=\"CitationRef\"\u003e7\u003c/span\u003e]. Juhe is currently widely used in formulas and the Chinese patent medicines, i.e., Huixiang Juhe Pill[\u003cspan citationid=\"CR8\" class=\"CitationRef\"\u003e8\u003c/span\u003e], Juhe Ezhu Granules[\u003cspan citationid=\"CR9\" class=\"CitationRef\"\u003e9\u003c/span\u003e], and Lujiao Juhe soup[\u003cspan citationid=\"CR10\" class=\"CitationRef\"\u003e10\u003c/span\u003e]. Juhe exhibits obvious effects when treating breast hyperplasia[\u003cspan citationid=\"CR11\" class=\"CitationRef\"\u003e11\u003c/span\u003e]. Breast hyperplasia mainly occurs in middle-aged women, accounting for 70% of breast diseases[\u003cspan citationid=\"CR12\" class=\"CitationRef\"\u003e12\u003c/span\u003e], and greatly harming women\u0026rsquo;s physical and mental health. In recent years, Juhe has been used to treat acute mastitis and breast hyperplasia, demonstrating significant effects[\u003cspan citationid=\"CR13\" class=\"CitationRef\"\u003e13\u003c/span\u003e, \u003cspan citationid=\"CR14\" class=\"CitationRef\"\u003e14\u003c/span\u003e]; thus, significant attention has been given to its medicinal value.\u003c/p\u003e \u003cp\u003eCitrus is the largest fruit in the world, and China is the second largest citrus producer in the world. In total, 19 provinces of China cultivate citrus, such as Jiangxi, Sichuan, and Guangxi[\u003cspan citationid=\"CR15\" class=\"CitationRef\"\u003e15\u003c/span\u003e], and the average annual output in recent years has reached 37.052\u0026nbsp;million tons[\u003cspan citationid=\"CR16\" class=\"CitationRef\"\u003e16\u003c/span\u003e]. At present, dozens of citrus varieties, such as \u003cem\u003eC. Tangerina\u003c/em\u003e Hort. Et Tanaka, \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Tangerina\u0026rsquo;, \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo;, \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;, \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Chachi\u0026rsquo;, \u003cem\u003eC. Reticulata\u003c/em\u003e Blanco cv. Zhangshuensi, and \u003cem\u003eC. reticulata\u003c/em\u003e Blanco cv. Kinokuni, are the main source of Juhe. With the continuous appearance of new varieties, significant differences in Juhe have been noted greatly: ①TMV have been gradually eliminated due to: intolerance to storage, low economic benefits and a lack of market competitiveness. TMV, such as \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; and \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Tangerina\u0026rsquo;, are gradually replaced by new citrus varieties, such as \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Tangerina\u0026rsquo; and \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo;, which have high economic benefits, less and tending to no seeds; only a few fruit farmers plant \u003cem\u003eC. retic\u0026micro;lata\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;for their own consumption. ②Juhe quality issues, due to the use of new fruit veriaties, are prominent. Thus as a multisource medicine, traditional Juhe is facing a sharp decline in source and quantity. At the same time, Juhe derived from new varieties is used directly without evaluation, resulting severe quality problems, and difficult to guarantee clinical efficacy.\u003c/p\u003e \u003cp\u003eCitrus is a versatile species. The peel can be used as \u003cem\u003eC. reticulatae\u003c/em\u003e Pericarpium (Dried Citrus Peel), the dry vascular bundle group can be used as the traditional Chinese medicine Juluo, the pulp can be eaten as fruit, and the seeds are used as Juhe. Most studies on citrus focused on the selection of new fruit varieties, the chemical composition of \u003cem\u003eC. reticulatae\u003c/em\u003e Pericarpium[\u003cspan citationid=\"CR17\" class=\"CitationRef\"\u003e17\u003c/span\u003e] and its pharmacological effects[\u003cspan citationid=\"CR18\" class=\"CitationRef\"\u003e18\u003c/span\u003e]. Furthermore, research on Juhe has exclusively focused on one type of certain chemical component, such as limonoids[\u003cspan citationid=\"CR19\" class=\"CitationRef\"\u003e19\u003c/span\u003e], but research on whether new varieties can replace TMV is rarely reported. To ensure quality and to identify new medical sources, this study intends to study Juhe based the following aspects: 1) genetic material; 2) shape and size; 3) flavonoids content.\u003c/p\u003e \u003cp\u003eTherefore, this study used different sources of Juhe as research material, employed SRAP molecular marker technology to analyze the genetic diversity combined with stereo microscope technology to quantify the characteristics and sizes, and applied HPLC technology to measure flavonoids content and quantity. Through the multidimensional evaluation model of genetic diversity-origin-trait-chemical characterization, we explored the distribution and change of active ingredients in different Juhe varieties according to plant kinship, and explained the differences in traits and metabolites of different varieties, and finally be able to:1) establishe a scientific, advanced and practical quality evaluation system to promote and enhance the improvement of quality standards; 2) select excellent Juhe resources and lay the foundation for the sustainable use of Juhe; 3) provide a scientific theoretical basis for the quality evaluation of other medicinal and edible dual-use varieties.\u003c/p\u003e "},{"header":"Materials And Methods","content":"\u003cdiv id=\"Sec3\" class=\"Section2\"\u003e\n\u003ch2\u003eSample collection\u003c/h2\u003e\n\u003cp\u003eFrom October to December 2015, samples were collected from 14 regions in Sichuan\u0026rsquo;s main citrus producing regions, including Luzhou, Guangyuan, Yibin, Zigong and so on (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e). Seeds (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e), picked out from the fruit, and leaves, which were cleaned with absolute ethanol, were stored in a refrigerator at -80\u0026nbsp;\u0026deg;C until use. The details of sample collection are shown in Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e1\u003c/span\u003e.\u0026nbsp;\u003c/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAnalysis of genetic diversity of Juhe\u003c/strong\u003e\u003c/h2\u003e\n\u003cp\u003eTotal DNA was extracted from the leaves according to method 2 in the instruction of the OMEGA Biological Plant DNA Extraction Kit (Omega Bio-tek, Norcross, GA, U.S.), and then its quality was assessed via 2.0% agarose gel electrophoresis. Total DNA was amplified by SRAP-PCR(94\u0026nbsp;\u0026deg;C for 5\u0026nbsp;min; 5 cycles of 94\u0026nbsp;\u0026deg;C for 5\u0026nbsp;min, 35\u0026nbsp;\u0026deg;C for 1\u0026nbsp;min, 72\u0026nbsp;\u0026deg;C for 1.5\u0026nbsp;min; 35 cycles of 94\u0026nbsp;\u0026deg;C for 5\u0026nbsp;min, 51.2\u0026nbsp;\u0026deg;C for 1\u0026nbsp;min, 72\u0026nbsp;\u0026deg;C for 1.5\u0026nbsp;min; and 72\u0026nbsp;\u0026deg;C extension for 10\u0026nbsp;min) 80 primer pairs (Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e2\u003c/span\u003e)[\u003cspan class=\"CitationRef\"\u003e20\u003c/span\u003e]. The 20 \u0026micro;L of PCR, mixture contains 10 \u0026micro;L of 2\u0026thinsp;\u0026times;\u0026thinsp;Taq PCR MasterMix, 2 \u0026micro;L of primers, 2 \u0026micro;L of DNA template, and 6 \u0026micro;L of dd H\u003csub\u003e2\u003c/sub\u003eO. After amplification, electrophoresis was performed on a 2% agarose gel, and products were detected with UV. In total, 22 pairs of primers with good amplification were selected from 80 primers.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec4\" class=\"Section2\"\u003e\n\u003ch2\u003eAnalysis of Juhe shape and size\u003c/h2\u003e\n\u003cp\u003eAn X-ray imaging system was used to detect Juhe embryos (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e), and samples with intact seed embryos were used for subsequent experiments.\u003c/p\u003e\n\u003cp\u003eThirty citrus seeds were randomly obtained from each batch of samples. The length of the seed ridge line was recorded as the long, the longest line perpendicular to the seed ridge line was recorded as the wide, and the vertical line from the width to the anti-species was recorded as the height. These three parameters were measured using a stereo microscope. The size of the seed was represented by the sum of the length, width, and height (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003e). The seed\u0026rsquo;s overall variance was used to measure the shape, the smaller the overall variance, the closer the shape is to a sphere (overall variance\u0026thinsp;\u0026gt;\u0026thinsp;0.05)[\u003cspan class=\"CitationRef\"\u003e21\u003c/span\u003e\u0026ndash;\u003cspan class=\"CitationRef\"\u003e22\u003c/span\u003e]. Otherwise the shape are considered to an ovoid. The following formula was used to calculate the population variance:\u003c/p\u003e\n\u003cp\u003e[3(x\u0026nbsp;\u003csub\u003elength\u003c/sub\u003e \u003csup\u003e2\u003c/sup\u003e+ x\u0026nbsp;\u003csub\u003ewidth\u003c/sub\u003e \u003csup\u003e2\u003c/sup\u003e+ x\u0026nbsp;\u003csub\u003eheight\u003c/sub\u003e \u003csup\u003e2\u003c/sup\u003e)-(x\u0026nbsp;\u003csub\u003elength\u003c/sub\u003e+ x\u0026nbsp;\u003csub\u003ewidth\u003c/sub\u003e +x\u0026nbsp;\u003csub\u003eheight\u003c/sub\u003e)\u003csup\u003e2\u003c/sup\u003e]/3\u003csup\u003e2\u003c/sup\u003e\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec5\" class=\"Section2\"\u003e\n\u003ch2\u003eDetermination of flavonoids\u003c/h2\u003e\n\u003cp\u003eNaringin, hesperidin and neohesperidin levels were assessed. Methanol was used to make standard solutions of 0.14\u0026nbsp;mg/mL, 0.10\u0026nbsp;mg/mL, and 0.12\u0026nbsp;mg/mL. Briefly, 0.5000\u0026nbsp;g of Juhe powder was mixed with 25\u0026nbsp;mL methanol. The mixture was shaken well, and then extracted at reflux for 1.5\u0026nbsp;h in a 70\u0026nbsp;\u0026deg;C water bath. Then, the solution was cooled and filtered through a 0.45\u0026nbsp;\u0026micro;m microporous filter to obtain the test solution.\u003c/p\u003e\n\u003cp\u003eFlavonoids determination was performed on Dikma C18 reversed-phase column (250 mm\u0026thinsp;\u0026times;\u0026thinsp;4.6\u0026nbsp;mm, 5\u0026nbsp;\u0026micro;m). The solvent system was acetonitrile/0.1% phosphoric acid\u0026thinsp;=\u0026thinsp;at a 20:80 ratio. The following parameters were employed: flow rate, 1.0\u0026nbsp;mL/min; detection wavelength, 283\u0026nbsp;nm ; column temperature, 30\u0026nbsp;\u0026deg;C and sample amount, 10 \u0026micro;L[\u003cspan class=\"CitationRef\"\u003e23\u003c/span\u003e].\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec6\" class=\"Section2\"\u003e\n\u003ch2\u003eStatistical analysis\u003c/h2\u003e\n\u003cp\u003eNTsys 2.10e software was used to calculate the Dice genetic similarity coefficient, and the unweighted group paired arithmetic mean method (UPGMA) was used for genetic diversity and cluster analysis. IBM SPSS Statistics 21 software was used for analysis of the following data: SRAP molecularly labeled (1,0) matrix data, seed size and shape, and flavonoids content.\u003c/p\u003e\n\u003c/div\u003e"},{"header":"Results","content":"\u003cdiv id=\"Sec8\" class=\"Section2\"\u003e\n\u003ch2\u003eAnalysis of Juhe genetic diversity\u003c/h2\u003e\n\u003cp\u003eDNA bands were single, clear, bright, and nontailing, indicating that intact DNA was obtainted at high yields (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e5\u003c/span\u003e). In this study, 22 pairs of primers with good amplification efficiency were selected, and genetic polymorphisms of 35 Juhe samples were studied. Obvious differences in SRAP marker polymorphisms were noted. There are 159 clear bands were obtained from this experiment (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e6\u003c/span\u003e).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eGenetic diversity analysis was performed on the (1,0) matrix data obtained by SRAP labeling of 35 Juhe samples (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e7\u003c/span\u003e). The genetic similarity coefficient ranged from 0.73 to 0.94. Sample No. 23 exhibited the lowest relationship with other 34 samples, and the genetic similarity coefficient was 0.73. Base on a genetic similarity coefficient of 0.816, the four samples collected in Nanchong were grouped into one category. The remaining 30 samples had similarity coefficients ranging from 0.82 to 0.93. The genetic relationship is the closest for samples No. 17 and 18, with a genetic similarity coefficient of 0.932. Overall, the relationship between \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;ponkan\u0026rsquo; and \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; is the closest, and \u003cem\u003eFortunella margarita\u003c/em\u003e (Lour.) Swingle exhibits the lowest relationship with \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec9\" class=\"Section2\"\u003e\n\u003ch2\u003eAnalysis of Juhe shape and size\u003c/h2\u003e\n\u003cp\u003eThe total variance of the 35 batches of orange core samples is greater than 0.05, and the sum of the three dimensions is generally the same (Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e3\u003c/span\u003e). The shapes of samples of different varieties from different regions exhibit great differences, and samples of the same variety from different regions also exhibit great differences. The shapes of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo;, \u003cem\u003eCitrus reticulata\u003c/em\u003e and \u003cem\u003eC. ichangensis\u003c/em\u003e Swingle seeds are similar to those of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;.\u003c/p\u003e\n\u003cp\u003eSeeds from different varieties and origins exhibit no significant differences in the size. The size of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; seeds is similar to \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; seeds. However, the seed sizes of \u003cem\u003eC. sinensis\u003c/em\u003e, \u003cem\u003eC. reticulata\u003c/em\u003e, \u003cem\u003eC.\u003c/em\u003e (L.) Osbeck, and \u003cem\u003eC. ichangensis\u003c/em\u003e Swingle are less similer to that of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec10\" class=\"Section2\"\u003e\n\u003ch2\u003eDetermination of flavonoids content\u003c/h2\u003e\n\u003cp\u003eNaringin, hesperidin and neohesperidin levels in 35 Juhe samples were determined (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e8\u003c/span\u003e). The content is shown in Table\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e4\u003c/span\u003e, and the chromatogram is shown in Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e8\u003c/span\u003e. Naringin and neohesperidin levels were relatively low in all samples, and only a limited number of some \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; samples and \u003cem\u003eFortunella margarita\u003c/em\u003e (Lour.) Swingle samples contain naringin. The percentage content of hesperidin exhibits the following trend: \u003cem\u003eC. sinensis\u003c/em\u003e\u0026thinsp;\u0026gt;\u0026thinsp;\u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;\u0026gt; \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo;\u0026gt; \u003cem\u003eFortunella margarita\u003c/em\u003e (Lour.) Swingle. Five batches of \u003cem\u003eC. sinensis\u003c/em\u003e samples (No. 3, No. 22, No. 15, No. 25, and No. 31), 2 batches of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; (No.34 and No.12) and 1 batch of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; (No. 18) contain no neohesperidin.\u003c/p\u003e\n\u003c/div\u003e\n\u003cdiv id=\"Sec11\" class=\"Section2\"\u003e\n\u003ch2\u003eCorrelation analysis of genetic diversity, trait size and flavonoid content in Juhe\u003c/h2\u003e\n\u003cp\u003eSRAP molecular marker clustering analysis showed that the relationship between \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; from different origins and \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; from different origins was closer than other varieties. The shape and size of different samples were measured using a stereo microscope. Juhe samples of the same variety from different origins exhibits differences in size and shape, and the Juhe samples of different varieties and origins also exhibited differences. HPLC determination of flavonoids showed that only a portion of the \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; samples exhibited composition similar to \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;.\u003c/p\u003e\n\u003cp\u003eCluster analysis was performed on these three sets of data (Fig.\u0026nbsp;\u003cspan class=\"InternalRef\"\u003e9\u003c/span\u003e). The results showed that sample No. 30 had diverged the most from other samples. Among the 12 samples of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;, except for No. 7, No. 8, and No. 9, had close relationships, and the relationships among samples of No. 14, No. 20, No. 24, and No. 28 were closer. \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; No. 2 and No. 6; No. 12 and No. 13; No. 17, 19, 21, 26, 27, 32 and 34 were grouped into one class separately. The other samples, with the exception of No. 3, No. 10 and No. 14, were grouped into one group, and these samples were relatively dispersed and not closely to each other. In general, the relationship between different varieties and \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; exhibited the following trend: \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; \u0026gt; \u003cem\u003eC. sinensis\u003c/em\u003e, \u003cem\u003eC.\u003c/em\u003e (L.) Osbeck\u0026thinsp;\u0026gt;\u0026thinsp;\u003cem\u003eC. ichangensis\u003c/em\u003e Swingle, \u003cem\u003eC. reticulata\u003c/em\u003e and \u003cem\u003eFortunella margarita\u003c/em\u003e (Lour.) Swingle.\u003c/p\u003e\n\u003c/div\u003e"},{"header":"Discussion","content":" \u003cp\u003eIn total, 35 batches of Juhe samples were collected in this study, including 12 TMV, \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;, and 23\u0026nbsp;N-TMV. The results showed that among 35 samples, \u003cem\u003eC. sinensis\u003c/em\u003e, \u003cem\u003eC.\u003c/em\u003e (L.) Osbeck, \u003cem\u003eC. ichangensis\u003c/em\u003e Swingle, \u003cem\u003eC. reticulata\u003c/em\u003e and \u003cem\u003eFortunella margarita\u003c/em\u003e (Lour.) Swingle significantly differe from the traditional medicinal variety \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; based on relationship,seed shape and size, and flavonoids content. \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; and \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; exhibit the highest similarity based on these three aspects, thus, suggesting the need for further researched on this variety as an emerging variety for medical use.\u003c/p\u003e \u003cp\u003eRFLP, RAPD, SSR and SCAR are molecular marker methods usually used to study citrus kinship, but the research found that these research methods have limitations[\u003cspan citationid=\"CR24\" class=\"CitationRef\"\u003e24\u003c/span\u003e], while the SRAP (sequence-related amplified polymorphism)[\u003cspan citationid=\"CR25\" class=\"CitationRef\"\u003e25\u003c/span\u003e] is Stable, repeatable, short cycle, good polymorphism, which are widely used in the study of genetic diversity and genetic relationship of plant germplasm resources. Therefore, the SRAP molecular marker method was used in this study. Research shows that different varieties of citrus have distant genetic relationships with \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;. This finding may be attributed to the fact that \u003cem\u003eFortunella margarita\u003c/em\u003e (Lour.) Swingle belongs to Fortunella Swingle family, whereas the remaining samples belong to citrus L.. \u003cem\u003eC. reticulata\u003c/em\u003e is an artificial hybrids that is gererated from Valencia orange as the female parent and Jiangnan orange or zhusha orange as the male parent. \u003cem\u003eC. sinensis\u003c/em\u003e is a type of \u003cem\u003eC.\u003c/em\u003e (L.) Osbeck[\u003cspan citationid=\"CR26\" class=\"CitationRef\"\u003e26\u003c/span\u003e], that was originally produced in the United States and were introduced from Morocco and other countries[\u003cspan citationid=\"CR27\" class=\"CitationRef\"\u003e27\u003c/span\u003e] to China in 1965 and later. \u003cem\u003eC. ichangensis\u003c/em\u003e Swingle is a primitive variety in Sichuan, that potantially originated in the Quaternary Ice Age and is distantly related other citrus varieties[\u003cspan citationid=\"CR28\" class=\"CitationRef\"\u003e28\u003c/span\u003e, \u003cspan citationid=\"CR29\" class=\"CitationRef\"\u003e29\u003c/span\u003e]. The r close relationship between \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; and \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; is potentially explained by the fact that both belong to the \u003cem\u003eC. reticulata\u003c/em\u003e Blanco. Furthermore, \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; was grafted on \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;, and genetic material exchange potentially occurred\u003csup\u003e[30]\u003c/sup\u003e. The genetic relationship between different variety samples from the same place of origin is closer, thus, the same genetic mutations may occur among different varieties due to similar environmental factors. In addition, citrus varieties easily hybridize[\u003cspan citationid=\"CR31\" class=\"CitationRef\"\u003e31\u003c/span\u003e], thus increasing the similarity in genetic material. Given the long planting history and different genetic mutations, the relationship between \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; samples is not similar. Natural environmental factors, such as the climate, soil, and altitude of Nanchong, are particularly suitable for the growth of citrus plants. Therefore, samples of different varieties in Nanchong are less related to the other samples, and were divided into a separate branch.\u003c/p\u003e \u003cp\u003eSince ancient times, Chinese medicine scholars believe that the characteristics of Chinese material medica, such as shape, size and odor, were used as identification characteristics and quality standards. However, human-dependent detection is strongly dependent on personal experience and subjectivie. Therefore, stereomicroscopes are often used to observe the morphological structure of some traditional Chinese medicines. For example, Jie Zhang used stereomicroscopes to identify the seeds of \u003cem\u003eAtractylodes macrocephala\u003c/em\u003e and \u003cem\u003eAtractylodes lancea\u003c/em\u003e[\u003cspan citationid=\"CR32\" class=\"CitationRef\"\u003e32\u003c/span\u003e]; Xiaolin Li studied the morphological structure of \u003cem\u003eSalvia miltiorrhiza\u003c/em\u003e seeds using a stereomicroscopes[\u003cspan citationid=\"CR33\" class=\"CitationRef\"\u003e33\u003c/span\u003e]. Each version of the \u003cem\u003eChinese Pharmacopoeia\u003c/em\u003e has records regulations on the size and shape of Juhe. Therefore, in this study we use a stereomicroscope to measure the size and shape. This technique presents Juhe\u0026rsquo;s appearance characteristics in a digital form, and avoids subjective error. The study found that Juhe collected from different regions, even in the same variety, exhibit different sizes and shapes, which may be due to different natural factors, such as the sea level, climate conditions, differences in cultivation techniques, cross pollination with other varieties, or different growth periods. Discrepancies in different varieties may be caused by genetic differences.\u003c/p\u003e \u003cp\u003eThe main active components of tangerine kernels are limonoids and flavonoids. information on limonoids can be found in our previous reports. Our experiments demonstrated that \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; limonin compounds are closest to \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;. In this experiment, we first determined hesperidin, naringin, and neohesperidin levels in Juhe. Naringin only exists in some \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; samples and \u003cem\u003eFortunella margarita\u003c/em\u003e (Lour.) Swingle sample. Most \u003cem\u003eC. sinensis\u003c/em\u003e samples do not contain neohesperidin, This finding may be due to inherent factors such as genetic material and seed maturity[\u003cspan citationid=\"CR34\" class=\"CitationRef\"\u003e34\u003c/span\u003e\u0026ndash;\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e]. Some \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; samples lack naringin and a portion of \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; samples lack of neohesperidin. These finding may be attributed to their growth environment and studies have shown that some environmental factors, such as light[\u003cspan citationid=\"CR36\" class=\"CitationRef\"\u003e36\u003c/span\u003e], temperature, irrigation[\u003cspan citationid=\"CR37\" class=\"CitationRef\"\u003e37\u003c/span\u003e], and fertilization[\u003cspan citationid=\"CR38\" class=\"CitationRef\"\u003e38\u003c/span\u003e], can affect flavonoids content in plant seeds. We also found that flavonoids content in \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; and \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; samples from some places was similar, which was consistent with the research results base on shape and size. This funding may indicate that the seeds\u0026rsquo; chemical composition was closely related to their physicochemical properties[\u003cspan citationid=\"CR35\" class=\"CitationRef\"\u003e35\u003c/span\u003e], which is consistent with previous research and further illustrates that \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; is the most equivalent to \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;, which proves that \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; is a qualified emerging medical variety.\u003c/p\u003e \u003cp\u003eCluster analysis revealed close relationships between \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; samples from Neijiang, Ziyang, Zigong, and Meishan, and the relationships among the \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; samples obtained from these four places are also close. These four cities are geographically adjacent, indicating that mutual introduction may exist. Given similar natural conditions and cultivation techniques, the relationship remains close after many years of cultivation.\u003c/p\u003e \u003cp\u003eIn general, most \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; samples are closely related to the \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; samples, indicating that \u003cem\u003eC\u003c/em\u003e. \u0026lsquo;Ponkan\u0026rsquo; has the potential to become a new Chinese medicinal material.\u003c/p\u003e "},{"header":"Conclusions","content":"\u003cp\u003eIn recent years, many new citrus varieties emerged, but new varieties of Juhe were used for medicinal purposes without scientific research. The 2015 edition of \u003cem\u003eChinese Pharmacopoeia\u003c/em\u003e does not further specify the cultivation variants that could be used as the source of Juhe. In addition, quality standards could not objectively evaluate the quality differences between different Juhe sources. However, TMV differ greatly from N-TMV based on relationship, traits and active ingredients, TMV have more seeds than N-TMV. Five \u003cem\u003eC\u003c/em\u003e. \u0026lsquo;Ponkan\u0026rsquo; samples, including Zigong No. 17, Meishan No. 21, Neijiang No. 27, Nanchong No. 32, and JiangyouNo.34 were similar to \u003cem\u003eC.\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;, can be used as original Juhe varieties for further medicinal research.\u003c/p\u003e\n\u003cp\u003eTo ensure the quality and efficacy of Juhe, this study first explored the equivalence between TMV and N-TMV, and established an evaluation model of \u0026ldquo;genetic diversity-origin-trait-chemical characterization\u0026rdquo; from the perspective of modern biology and traditional Chinese medicine. The evaluation model could be used to further improve quality standards of Juhe. The study found that citrus cultivation techniques should be further standardized to reduce quality differences. Moreover, many traditional Chinese medicines are facing a shortage of resources[\u003cspan class=\"CitationRef\"\u003e39\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e40\u003c/span\u003e], so cultivating new varieties has become a new trend[\u003cspan class=\"CitationRef\"\u003e41\u003c/span\u003e, \u003cspan class=\"CitationRef\"\u003e42\u003c/span\u003e], and this study provides idea for the studies of other traditional Chinese medicines.\u003c/p\u003e"},{"header":"Additional file","content":"\u003cp\u003e\u003cstrong\u003eAdditional file 1: Table S1.\u003c/strong\u003e 35 batches of samples\u0026rsquo; numbers, varieties and collection places. \u003cstrong\u003eTable S2.\u003c/strong\u003e Calibration curve of three tested compounds. \u003cstrong\u003eTable S3.\u003c/strong\u003e Stability, repeatability, precision and recovery rate of four tested compounds. \u003cstrong\u003eFig. S1.\u003c/strong\u003e The X-ray images of 35 batches of samples.\u003c/p\u003e"},{"header":"Abbreviations","content":"\u003cp\u003eTMV: traditional medicinal varieties. N-TMV: nontraditional medicinal varieties. SRAP: sequence-related amplified polymorphism. RFLP: restriction fragment length polymorphism. RAPD: random amplified polymorphic DNA, SSR: simple sequence repeats. SCAR: sequence characterized amplified regions. HPLC: high performance liquid chromatography. \u003cem\u003eC\u003c/em\u003e. \u0026lsquo;Dahongpao\u0026rsquo;: \u003cem\u003eCitrus reticulat \u0026lsquo;\u003c/em\u003eDahongpao\u0026rsquo;. \u003cem\u003eC\u003c/em\u003e. reticulate: \u003cem\u003eCitrus reticulate. C\u003c/em\u003e. sinensis: \u003cem\u003eCitrus sinensis.\u003c/em\u003e \u003cem\u003eC\u003c/em\u003e. \u0026lsquo;Ponkan\u0026rsquo;: \u003cem\u003eCitrus \u003c/em\u003e\u0026lsquo;Ponkan\u0026rsquo;. \u003cem\u003eC. \u003c/em\u003e(L.) Osbeck: \u003cem\u003eCitrus \u003c/em\u003e(L.) Osbeck. \u003cem\u003eC. ichangensis\u003c/em\u003e Swingle: \u003cem\u003eCitrus ichangensis\u003c/em\u003e Swingle. \u003cem\u003eC. Tangerina\u003c/em\u003e Hort. Et Tanaka: \u003cem\u003eCitrus Tangerina\u003c/em\u003e Hort. Et Tanaka. \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Tangerina\u0026rsquo;: \u003cem\u003eCitrus reticulata\u003c/em\u003e \u0026lsquo;Tangerina\u0026rsquo;. \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Chachi\u0026rsquo;: \u003cem\u003eCitrus reticulata\u003c/em\u003e \u0026lsquo;Chachi\u0026rsquo;. \u003cem\u003eC. Reticulata\u003c/em\u003e Blanco cv. Zhangshuensi: \u003cem\u003eCitrus Reticulata\u003c/em\u003e Blanco cv. Zhangshuensi. \u003cem\u003eC. reticulata\u003c/em\u003e Blanco cv. Kinokuni: \u003cem\u003eCitrus reticulata\u003c/em\u003e Blanco cv. Kinokuni. ABZ: Aba Zhou. BZ: Bazhong. CD: Chengdu. DY: Deyang. DZ: Dazhou. GA: Guangan. GY: Guangyuan. GZZ: Ganzi Zhou. LS: Leshan. LSZ: Liangshang Zhou. LZ: Luzhou. MY: Mianyang. MS:Meishan. NC: Nanchong. NJ: Neijiang. PZH: Panzhihua. SN:Suining. YA: Yaan. YB:Yibin. ZG: Zigong. ZY: Ziyang\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eEthics approval and consent to participate\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConsent to publish\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNot applicable\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAvailability of data and materials\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eMost of the data generated of analyzed during the study are included in this article and its Additional file 1.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors declare no competing financial interest.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThis research was supported by the National Natural Science Foundation of China (Grant No. 81703654), and the Talent Project of National Natural Science Foundation of China (Grant No. J131003340-13).\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor Contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eConceptualization, Jin Pei; data curation, Li Wang and Rui Wang; formal analysis, Cuiping Chen; funding acquisition, Qinghua Wu; Investigation, Bin Xian and Li Wang; methodology, Chaoxiang Ren and Jiang Chen; software, Wanting ; Writing \u0026ndash; original draft, Bin Xian; writing \u0026ndash; review \u0026amp; editing, Qinghua Wu and Jin Pei. All authors have read and approved the manuscript.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAcknowledgments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eWe thank everyone who gave us inspiration, analytical methods, and revisions. Thanks to secure AJE for making the English expression of the article more accurate.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthor information\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003csup\u003e1 \u003c/sup\u003ePharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, Sichuan.\u003c/p\u003e\n\u003cp\u003e\u003csup\u003e2 \u003c/sup\u003eState Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu 611137, Sichuan.\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\u003cli\u003e \u003cspan\u003eNational Pharmacopoeia Commission. Chinese Pharmacopoeia. 397. 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Journal of Pharmaceutical Research. 2019;38(5):295\u0026ndash;8.\u003c/span\u003e \u003c/li\u003e \u003cli\u003e \u003cspan\u003eLiu JH, Wei JW, Liu GY. Current situation and future of sustainable development of traditional chinese medicine resources. World Latest Medicine Information. 2019;19(9):32\u0026ndash;3.\u003c/span\u003e \u003c/li\u003e \u003cli\u003e \u003cspan\u003eMeng XC, Du HW, Wei WF, Huo JH. Problems and countermeasures in development of Chinese materia medica resource. Chinese Traditional Herbal Drug. 2018;49(16):3735\u0026ndash;41.\u003c/span\u003e \u003c/li\u003e \u003cli\u003e \u003cspan\u003eZhu WQ, Wang LJ, Liang P, He ZL, Yan GH. Analysis of the status quo and future prospects of Chinese medicinal resources sustainable development. World Chinese Medicine. 2018;13(7):1752\u0026ndash;5.\u003c/span\u003e \u003c/li\u003e\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-indent: 8.75pt;line-height: 32px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cstrong\u003e\u003cspan style=\"font-size: 10px; line-height: 28px; font-family: Verdana, Geneva, sans-serif; color: rgb(0, 0, 0);\"\u003eTable 1 Sample collection\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"margin-left: -30.05pt;border-collapse: collapse;border: none;\" width=\"671\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: justify;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eNo.\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eVariety\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eplace\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-top-width: 1pt;border-right-width: 1pt;border-bottom-width: 1pt;border-style: solid solid solid none;border-top-color: windowtext;border-right-color: windowtext;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003edate\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: justify;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eNo.\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eVariety\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eplace\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003edate\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e1\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 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width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-11\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e19\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eYaan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-11\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e2\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan 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0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eMeishan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-13\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e3\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. sinensis\u003c/span\u003e\u003c/em\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eQionglai\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-11\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e21\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eMeishan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-13\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e4\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eLuzhou\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-14\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e22\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. sinensis\u003c/span\u003e\u003c/em\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eMeishan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-13\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e5\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. reticulata\u003c/span\u003e\u003c/em\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGuangyuan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-22\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e23\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eFortunella margarita\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;(Lour.) Swingle\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eChengdu\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-19\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e6\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGuangyuan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-22\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e24\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eZiyang\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-27\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e7\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGuangyuan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-22\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e25\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. sinensis\u003c/span\u003e\u003c/em\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eZiyang\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-27\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e8\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eLeshan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-27\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e26\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eZiyang\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-27\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e9\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eLeshan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-27\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e27\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eNeijiang\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: 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style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. sinensis\u003c/span\u003e\u003c/em\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eLeshan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-27\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e28\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eNeijiang\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-28\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e11\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eYibin\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, 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32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eNanchong\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-30\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e12\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e1\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eYibin\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-30\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e30\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. ichangensis\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px;\"\u003eSwingle\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eNanchong\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-30\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e13\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e2\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eYibin\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-31\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e31\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. sinensis\u003c/span\u003e\u003c/em\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eNanchong\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-30\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e14\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC.\u0026nbsp;\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px;\"\u003e(L.) Osbeck\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eYibin\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-31\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e32\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eNanchong\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-30\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e15\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px;\"\u003eC. sinensis\u003c/span\u003e\u003c/em\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eYibin\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-10-31\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e33\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border: none;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eSuining\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-12-01\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n 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sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eZigong\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-01\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border: none;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: 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padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eJiangyou\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-12-03\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border: none;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e17\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border: none;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u0026lsquo;Ponkan\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border: none;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eZigong\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-01\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n 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style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border: none;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eJiangyou\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border: none;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-12-03\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 27.7pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"5.514157973174367%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e18\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 92.8pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"18.47988077496274%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cem\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003eC.\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"line-height: 28px; border: 1pt none windowtext; padding: 0in;\"\u003e\u0026nbsp;\u003c/span\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026lsquo;Dahongpao\u0026rsquo;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"13.114754098360656%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eYaan\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.8pt;border-style: none solid solid none;border-bottom-width: 1pt;border-bottom-color: windowtext;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"13.263785394932937%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e2015-11-11\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 26pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"5.216095380029806%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 96.35pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"19.076005961251862%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.15pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"11.326378539493293%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.45pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"14.008941877794337%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style='margin: 0in 0in 0.0001pt;text-align: justify;text-indent: 24pt;line-height: 24px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin: 0in 0in 0.0001pt;text-indent: 24.1pt;line-height: 32px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cbr\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin: 0in 0in 0.0001pt;text-indent: 24.1pt;line-height: 32px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003eTable 2 The sequence of SRAP primers\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"border-collapse: collapse;border: none;\" width=\"625\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21.1pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eForward-Primer\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 160.5pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"34.24%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21.1pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e5\u0026rsquo;to3\u0026rsquo;\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 73.4pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"15.68%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21.1pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eReverse-Primer\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 163pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21.1pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e5\u0026rsquo;to3\u0026rsquo;\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eF1\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 160.5pt;border: none;padding: 0in 5.4pt;\" width=\"34.24%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eTGAGTCCAAACCGGATA\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 73.4pt;border: none;padding: 0in 5.4pt;\" width=\"15.68%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eR1\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 163pt;border: none;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTAAT\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eF2\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 160.5pt;border: none;padding: 0in 5.4pt;\" width=\"34.24%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eTGAGTCCAAACCGGAGC\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 73.4pt;border: none;padding: 0in 5.4pt;\" width=\"15.68%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eR2\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 163pt;border: none;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTTGC\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eF3\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 160.5pt;border: none;padding: 0in 5.4pt;\" width=\"34.24%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eTGAGTCCAAACCGGAAT\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 73.4pt;border: none;padding: 0in 5.4pt;\" width=\"15.68%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eR3\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 163pt;border: none;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTGAC\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n 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style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTTGA\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eF5\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 160.5pt;border: none;padding: 0in 5.4pt;\" width=\"34.24%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: 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style=\"width: 163pt;border: none;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTAAC\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 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style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eR6\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 163pt;border: none;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTGCA\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n 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style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTCAA\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eF8\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 160.5pt;border: none;padding: 0in 5.4pt;\" width=\"34.24%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: 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style=\"width: 163pt;border: none;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eGACTGCGTACGAATTCAC\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border: none;padding: 0in 5.4pt;\" width=\"15.36%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 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style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 163pt;border: none;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 71.7pt;border-style: none none solid;border-bottom-width: 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style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003eTGAGTCCAAACCGGAAA\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 73.4pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"15.68%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 21pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 163pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"34.72%\"\u003e\n 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21px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin: 0in 0in 0.0001pt;text-indent: 21.1pt;line-height: 24px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cbr\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin: 0in 0in 0.0001pt;text-indent: 21.1pt;line-height: 24px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 21px;\"\u003eTable 3\u0026nbsp;Data of shape, size (n=30)\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003ctable border=\"0\" cellpadding=\"0\" cellspacing=\"0\" style=\"width: 418.25pt;border-collapse: collapse;\" width=\"558\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 43.95pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"10.57347670250896%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eNo.\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.7pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.232974910394265%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eLong\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e（\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003emm\u003c/span\u003e\u003c/strong\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e）\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.8pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"16.845878136200717%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eWidth(mm)\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.5pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"13.799283154121865%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eHigh(mm)\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003evariance\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 119.05pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"28.49462365591398%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt -54.6pt;text-align: center;text-indent: 47.75pt;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eThree-dimensional sum\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 43.95pt;border: none;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"10.57347670250896%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e1\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.7pt;border: none;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.232974910394265%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e12.33\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.8pt;border: none;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"16.845878136200717%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e6.05\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.5pt;border: none;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"13.799283154121865%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e4.59\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;border: none;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e11.96\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 119.05pt;border: none;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"28.49462365591398%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e22.97\u0026plusmn;0.22\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 43.95pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"10.57347670250896%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e2\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.7pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.232974910394265%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e11.39\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.8pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"16.845878136200717%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: bottom;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e6.39\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.5pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"13.799283154121865%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e5.23\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e7.82\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 119.05pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"28.49462365591398%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: bottom;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e23.02\u0026plusmn;0.31\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 43.95pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"10.57347670250896%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e3\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.7pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.232974910394265%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e12.45\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.8pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"16.845878136200717%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e6.53\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.5pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"13.799283154121865%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e5.61\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 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28px;\"\u003e7.83\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e9.81\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 119.05pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"28.49462365591398%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e21.55\u0026plusmn;0.29\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 43.95pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"10.57347670250896%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e33\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.7pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.232974910394265%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e11.31\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.8pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"16.845878136200717%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e5.54\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.5pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"13.799283154121865%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e4.50\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e9.77\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 119.05pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"28.49462365591398%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e21.35\u0026plusmn;0.24\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 43.95pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"10.57347670250896%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e34\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.7pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.232974910394265%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e10.84\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.8pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"16.845878136200717%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e5.44\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.5pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"13.799283154121865%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e4.68\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e12.73\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 119.05pt;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"28.49462365591398%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e22.21\u0026plusmn;0.32\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 43.95pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"10.57347670250896%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e35\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.7pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.232974910394265%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e12.24\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.8pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"16.845878136200717%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e5.49\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 57.5pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"13.799283154121865%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e4.48\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.25pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"15.053763440860216%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e8.06\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 119.05pt;border-style: none none solid;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;height: 14.15pt;vertical-align: top;\" valign=\"top\" width=\"28.49462365591398%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e20.97\u0026plusmn;0.26\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 24pt;line-height: 24px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u0026nbsp;\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin: 0in 16.1pt 0.0001pt 0in;text-indent: 0in;line-height: 32px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cbr\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003cp style='margin: 0in 16.1pt 0.0001pt 0in;text-indent: 0in;line-height: 32px;font-family: \"Times New Roman\", serif;text-align: left;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003eTable 4 Flavonoids content of 35 Juhe samples (n=3)\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n\u003ctable border=\"1\" cellpadding=\"0\" cellspacing=\"0\" style=\"margin-left: -30.05pt;border-collapse: collapse;border: none;\" width=\"681\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 35.45pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"6.911764705882353%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003eNo.\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.8pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"12.5%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003eNaringin\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.9pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"13.970588235294118%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003eHesperidin\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.05pt;border-top-width: 1pt;border-right-width: 1pt;border-bottom-width: 1pt;border-style: solid solid solid none;border-top-color: windowtext;border-right-color: windowtext;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"16.61764705882353%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: justify;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003eNeohesperidin\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 35.4pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"6.911764705882353%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003eNo.\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.8pt;border-top-width: 1pt;border-style: solid none;border-top-color: windowtext;border-bottom-width: 1pt;border-bottom-color: windowtext;padding: 0in 5.4pt;\" width=\"12.5%\"\u003e\n \u003cp style='margin: 0in -16.2pt 0.0001pt 0in;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 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style=\"line-height: 28px;\"\u003e1\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.8pt;border: none;padding: 0in 5.4pt;\" width=\"12.5%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: middle;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e/\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.9pt;border: none;padding: 0in 5.4pt;\" width=\"13.970588235294118%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: middle;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e0.0631\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.05pt;border-style: none solid none none;border-right-width: 1pt;border-right-color: windowtext;padding: 0in 5.4pt;\" width=\"16.61764705882353%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: middle;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e0.0103\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 35.4pt;border: none;padding: 0in 5.4pt;\" width=\"6.911764705882353%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cstrong\u003e\u003cspan style=\"line-height: 28px;\"\u003e19\u003c/span\u003e\u003c/strong\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 63.8pt;border: none;padding: 0in 5.4pt;\" width=\"12.5%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: middle;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e/\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 70.9pt;border: none;padding: 0in 5.4pt;\" width=\"13.970588235294118%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: middle;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e0.0340\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 85.1pt;border: none;padding: 0in 5.4pt;\" width=\"16.61764705882353%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;vertical-align: middle;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 10px;\"\u003e\u003cspan style=\"line-height: 28px;\"\u003e0.0031\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 35.45pt;border: none;padding: 0in 5.4pt;\" width=\"6.911764705882353%\"\u003e\n \u003cp style='margin: 0in 0in 0.0001pt;text-align: center;text-indent: 0in;line-height: 32px;font-size:16px;font-family: \"Times New Roman\", serif;'\u003e\u003cspan style=\"color: rgb(0, 0, 0);\"\u003e\u003cspan style=\"font-family: Verdana, Geneva, sans-serif;\"\u003e\u003cspan style=\"font-size: 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\u003c/span\u003e\u003c/p\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"Citri reticulatae Semen, SRAP, shape and sizes, Flavonoids, nontraditional medicinal varieties","lastPublishedDoi":"10.21203/rs.3.rs-27851/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-27851/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003ch2\u003eBackground\u003c/h2\u003e \u003cp\u003eJuhe, which is derived from seeds of citrus (\u003cem\u003eCitri reticulata\u003c/em\u003e Semen), is wildly used as a traditional Chinese medicine. Its original plants have numinous variety, and new varieties are constantly emerging, especially in recent years. The decline in the market share of traditional varieties each year, has resulted in a shortage of Juhe resources, and nontraditional Juhe varieties have been used clinically without research. Thus Juhe resources vary significantly. Furthermore, most of new varieties are hybrids, with significant differences in genes, active ingredients. Therefore, a further research is needed on Nontraditional medicinal varieties.\u003c/p\u003e\u003ch2\u003eMethods\u003c/h2\u003e \u003cp\u003eIn this study, the genetic diversity, shape and size, and flavonoids content of 35 different batches of Juhe samples, from different source, were analyzed base on SRAP molecular markers, a stereo microscope, and HPLC. Then correlation analysis was performed using these data.\u003c/p\u003e\u003ch2\u003eResults\u003c/h2\u003e \u003cp\u003eThe genetic backgrounds of traditional medicinal varieties (TMV) and nontraditional medicinal varieties (N-TMV) differed significantly, and TMV were closely related to some \u003cem\u003eCitrus\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; samples. Seeds of TMV and N-TMV differ based on shape, size and number. In addition, flavonoid compounds and level in the seeds of TMV and N-TMV also showed a significant difference. Five batches of \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; samples collected from Zigong, Meishan, Neijiang, Nanchong and Jiangyou were closely related to the traditional medicinal variety \u003cem\u003eCitrus reticulata\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo;,based on genetic relationship, shape and sizes, as well as flavonoids content.\u003c/p\u003e\u003ch2\u003eConclusins:\u003c/h2\u003e \u003cp\u003eA total of 5\u0026nbsp;\u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Ponkan\u0026rsquo; samples, including Zigong No. 7, Meishan No. 21, Neijiang No. 27, Nanchong No. 32, and Jiangyou No. 34, were closely related to the traditional medicinal variety \u003cem\u003eC. reticulata\u003c/em\u003e \u0026lsquo;Dahongpao\u0026rsquo; in genetic relationship, seeds\u0026rsquo; characteristics and sizes, and flavonoids content. These varieties could serve as a new medicinal variety.\u003c/p\u003e","manuscriptTitle":"Assessing the equivalence between traditional medicinal varieties and nontraditional medicinal varieties of Citri reticulata Semen","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2020-05-14 21:50:56","doi":"10.21203/rs.3.rs-27851/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"f88537bb-f48f-43d4-ba6b-7c7e537a96a2","owner":[],"postedDate":"May 14th, 2020","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":100678,"name":"Plant Molecular Biology and Genetics"}],"tags":[],"updatedAt":"2020-09-07T14:49:26+00:00","versionOfRecord":[],"versionCreatedAt":"2020-05-14 21:50:56","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-27851","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-27851","identity":"rs-27851","version":["v1"]},"buildId":"_2-kVJe1T_tPrBINL-cwx","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}