Laser-Ablated Gold Nanoparticles: A Novel Approach to Antimicrobial Artificial Eyes

Laser-Ablated Gold Nanoparticles: A Novel Approach to Antimicrobial Artificial Eyes | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Laser-Ablated Gold Nanoparticles: A Novel Approach to Antimicrobial Artificial Eyes Alaa Mohamed, Ahmed Elgendy, Khaled Salah Eldin Abdelkader, Esraa Ahmed, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-8296941/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Patients with artificial eyes often suffer from severe, persistent microbial infections, particularly due to antibiotic-resistant strains such as methicillin-resistant Staphylococcus aureus (MRSA). Nowadays, gold nanoparticles (AuNPs) exhibit strong antimicrobial properties, offering a promising solution to this challenge. In this study, laser ablation was employed to produce AuNPs, which were incorporated into artificial eye materials during fabrication. The antimicrobial efficacy of laser-ablated AuNPs against resistant bacteria was systematically evaluated using multiple microbiological assays. A colony forming unit and growth kinetic analysis were determined using an ANOVA test. Moreover, Logistic and Gompertz models were performed to visualize the MRSA growth kinetic rate. Our results demonstrate that AuNPs significantly inhibit bacterial growth, suggesting their potential as an effective antimicrobial component in ocular prostheses. Theoretically, the Gompertz model was the best-fit model for experimental kinetic data. We have produced a foundational framework for integrating AuNPs into artificial eyes to combat infections, paving the way for the development of novel antimicrobial prostheses. Artificial eye Antimicrobial Activity Laser ablation gold nanoparticles MRSA Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. Our growing team is made up of researchers and industry professionals working together to solve the most critical problems facing scientific publishing. Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-8296941","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":558498519,"identity":"29167af0-4dcd-423b-b9d3-19a9728902e8","order_by":0,"name":"Alaa Mohamed","email":"","orcid":"","institution":"Beni-Suef University","correspondingAuthor":false,"prefix":"","firstName":"Alaa","middleName":"","lastName":"Mohamed","suffix":""},{"id":558498528,"identity":"b670e72a-c778-4e90-9c09-1b7fe02e09d6","order_by":1,"name":"Ahmed Elgendy","email":"","orcid":"","institution":"Beni-Suef 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Nowadays, gold nanoparticles (AuNPs) exhibit strong antimicrobial properties, offering a promising solution to this challenge. In this study, laser ablation was employed to produce AuNPs, which were incorporated into artificial eye materials during fabrication. The antimicrobial efficacy of laser-ablated AuNPs against resistant bacteria was systematically evaluated using multiple microbiological assays. A colony forming unit and growth kinetic analysis were determined using an ANOVA test. Moreover, Logistic and Gompertz models were performed to visualize the MRSA growth kinetic rate. Our results demonstrate that AuNPs significantly inhibit bacterial growth, suggesting their potential as an effective antimicrobial component in ocular prostheses. Theoretically, the Gompertz model was the best-fit model for experimental kinetic data. We have produced a foundational framework for integrating AuNPs into artificial eyes to combat infections, paving the way for the development of novel antimicrobial prostheses.\u003c/p\u003e","manuscriptTitle":"Laser-Ablated Gold Nanoparticles: A Novel Approach to Antimicrobial Artificial Eyes","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2025-12-15 15:12:03","doi":"10.21203/rs.3.rs-8296941/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"d1ba5e15-10a9-4f16-86d0-f0bd7a0097b2","owner":[],"postedDate":"December 15th, 2025","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[],"tags":[],"updatedAt":"2026-03-13T13:54:32+00:00","versionOfRecord":[],"versionCreatedAt":"2025-12-15 15:12:03","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-8296941","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-8296941","identity":"rs-8296941","version":["v1"]},"buildId":"8U1c8b4HqxoKbykW_rLl7","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}