Bacteroides rhinocerotis sp. nov., isolated from the fresh feces of rhinoceros in Beijing Zoo

A Gram˗stain˗negative, anaerobic, non˗motile, non˗spore˗forming, rod˗shaped bacterial strain named as NGMCC 1.200684 T was isolated from the fresh feces of rhinoceros in Beijing Zoo. Based on 16S rRNA gene sequences, phylogenetic analysis indicated that strain NGMCC 1.200684 T belonged to the genus Bacteroides and was most strongly related to the type strain of Bacteroides uniformis ATCC 8492 T (96.88%). The G+C content of the genomic DNA was determined to be 46.62 mol%. Between strains NGMCC 1.200684 T and Bacteroides uniformis ATCC 8492 T , the average nucleotide identity (ANI) and digital DNA˗DNA hybridization (dDDH) were 93.89 and 67.60%, respectively. Strain NGMCC 1.200684 T can produce acid from fermentation of several substrates, including glucose, mannitol, lactose, saccharose, maltose, salicin, xylose, cellobiose, mannose, raffinose, sorbitol, trehalose, D˗galactose，maltotriose. The major cellular fatty acids (>10 %) were identified as anteiso˗C 15:0 , iso˗C 15:0 , iso˗C 14:0 , iso˗C 17:0 3˗OH. The polar lipid profile of strain NGMCC 1.200684 T was determined to contain diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, two unknown aminophospholipids. Based on phenotypic, phylogenetic and chemotaxonomic characteristics, a novel species of the genus Bacteroides , Bacteroides rhinocerotis sp. nov. is proposed. The type strain is NGMCC 1.200684 T (=CGMCC 1.18013 T =JCM 35702 T ).