Design and initial characterization of fabricated composite scaffold dHPCTM-HPE (decellularized human placental connective tissue matrix - human placental extract)

Abstract Purpose The placenta, a prominent source of scaffolding material, has primarily been utilized in wound care. Enriched with numerous growth factors and extracellular matrix components, it holds the potential to create an exceptionally favorable environment for wound healing. The purpose of this study is to develop a combination product from placenta and evaluate the impact of processing method over bioactive molecules. Methods We developed a decellularized human placental connective tissue matrix (dHPCTM) and placental extract (HPE). These were combined in a 1:1 ratio to create a composite product known as dHPCTM-HPE. The fabricated products were obtained through processes involving homogenization, mechanical and chemical treatments, and freeze-drying. To ascertain the retention of structural and bioactive molecules within these products, we conducted quantification tests for amino acids, collagen, glycosamninoglycans, histological evaluation, porosity, and swelling ratio. Results The combined product, dHPCTM-HPE, exhibited superior biochemical and mechanical properties when compared to dHPCTM or HPE alone. Conclusion This combination shows promise for the treatment of chronic and deeper wounds where a single intervention is less likely to yield optimal results.