Discovery of the first Tn630 member and the closest homolog of IS630 from viruses

Discovery of the first Tn630 member and the closest homolog of IS630 from viruses | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Discovery of the first Tn630 member and the closest homolog of IS630 from viruses Guangyou Duan, Dongsheng Wei, Yutong Guo, Cihan Ruan, Jia Chang, and 5 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3890757/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Background: IS630/Tc1/ mariner (ITm) represents the most widely distributed superfamily of DNA transposons in nature. Currently, bioinformatics research on ITm membersprimarily involves collecting data of existing and emerging members and organizing them into new groups or families. Results: In the present study, our survey revealed that Tc1 and IS630 members have a broad host range, spanning across all six biological kingdoms (bacteria, fungi, plantae, animalia, archaea and protista) and viruses. The primary discoveries include the first Tn630 member — Tn630-NC1 and the closest homolog of IS630 from viruses— Tc1-C#1. By incorporating our discovery into existing knowledge, we proposed a model to elucidate the formation of composite transposons in bacteria. Conclusions: Organization of Tc1 and IS630 members into groups across biological kingdoms facilitates data collection for future research, particularly on their horizontal transfer between different kingdoms. The formation of composite transposons may result from the asymmetric degeneration of terminal inverted repeats. IS630 should be combined with Tc1 into a family IS630/Tc1. Furthermore, IS630 and its homologs could serve as a valuable resource to investigate horizontal gene transfer between gut bacteria and phages, opening up new avenues for research in this field. Bioinformatics Tn630 ITm mariner pogo HGT Full Text Additional Declarations The authors declare no competing interests. Supplementary Information is not available with this version. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3890757","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":268753277,"identity":"1d257343-abb6-4e94-9244-c32ea171faa0","order_by":0,"name":"Guangyou Duan","email":"","orcid":"","institution":"Qilu Normal University","correspondingAuthor":false,"prefix":"","firstName":"Guangyou","middleName":"","lastName":"Duan","suffix":""},{"id":268753278,"identity":"ed026ada-40d9-4de0-a263-8c9c4746db9d","order_by":1,"name":"Dongsheng Wei","email":"","orcid":"","institution":"Nankai 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10:19:59","currentVersionCode":1,"declarations":{"humanSubjects":false,"vertebrateSubjects":false,"conflictsOfInterestStatement":false,"humanSubjectEthicalGuidelines":false,"humanSubjectConsent":false,"humanSubjectClinicalTrial":false,"humanSubjectCaseReport":false,"vertebrateSubjectEthicalGuidelines":false},"doi":"10.21203/rs.3.rs-3890757/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-3890757/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":50101680,"identity":"9a9e9390-6a7e-4692-8f33-7b8caa807f4b","added_by":"auto","created_at":"2024-01-24 14:55:22","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":572642,"visible":true,"origin":"","legend":"","description":"","filename":"paper2.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3890757/v1_covered_3c03d73d-ff96-49b4-92ec-e0185118af81.pdf"}],"financialInterests":"\u003cp\u003eThe authors declare no competing interests.\u003c/p\u003e\n\u003cp\u003eSupplementary Information is not available with this version.\u003c/p\u003e","formattedTitle":"\u003cp\u003e\u003cstrong\u003eDiscovery of the first Tn630 member and the closest homolog of IS630 from viruses\u003c/strong\u003e\u003c/p\u003e","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":true,"hideJournal":true,"highlight":"","institution":"Nankai University","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research 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Currently, bioinformatics research on ITm membersprimarily involves collecting data of existing and emerging members and organizing them into new groups or families.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eResults:\u003c/strong\u003e In the present study, our survey revealed that Tc1 and IS630 members have a broad host range, spanning across all six biological kingdoms (bacteria, fungi, plantae, animalia, archaea and protista) and viruses. The primary discoveries include the first Tn630 member — Tn630-NC1 and the closest homolog of IS630 from viruses— Tc1-C#1. By incorporating our discovery into existing knowledge, we proposed a model to elucidate the formation of composite transposons in bacteria.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConclusions:\u003c/strong\u003e Organization of Tc1 and IS630 members into groups across biological kingdoms facilitates data collection for future research, particularly on their horizontal transfer between different kingdoms. The formation of composite transposons may result from the asymmetric degeneration of terminal inverted repeats. IS630 should be combined with Tc1 into a family IS630/Tc1. Furthermore, IS630 and its homologs could serve as a valuable resource to investigate horizontal gene transfer between gut bacteria and phages, opening up new avenues for research in this field.\u003c/p\u003e","manuscriptTitle":"Discovery of the first Tn630 member and the closest homolog of IS630 from viruses","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-01-24 14:47:11","doi":"10.21203/rs.3.rs-3890757/v1","editorialEvents":[{"type":"communityComments","content":0}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true}}],"origin":"","ownerIdentity":"896862cb-feac-48dc-82e5-db229a996855","owner":[],"postedDate":"January 24th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"posted","subjectAreas":[{"id":28311011,"name":"Bioinformatics"}],"tags":[],"updatedAt":"2024-01-24T14:47:11+00:00","versionOfRecord":[],"versionCreatedAt":"2024-01-24 14:47:11","video":"","vorDoi":"","vorDoiUrl":"","workflowStages":[]},"version":"v1","identity":"rs-3890757","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3890757","identity":"rs-3890757","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}