Transcriptomic and Lipidomic Profiling Reveals Rapid Molecular Responses Associated with Thermotolerance in Chia (Salvia hispanica L.) Leaves

Transcriptomic and Lipidomic Profiling Reveals Rapid Molecular Responses Associated with Thermotolerance in Chia (Salvia hispanica L.) Leaves | Authorea try { document.documentElement.classList.add('js'); } catch (e) { } var _gaq = _gaq || []; _gaq.push(['_setAccount', 'G-8VDV14Y67G']); _gaq.push(['_trackPageview']); (function() { var ga = document.createElement('script'); ga.type = 'text/javascript'; ga.async = true; ga.src = ('https:' == document.location.protocol ? 'https://ssl' : 'http://www') + '.google-analytics.com/ga.js'; var s = document.getElementsByTagName('script')[0]; s.parentNode.insertBefore(ga, s); })(); Skip to main content Preprints Collections Wiley Open Research IET Open Research Ecological Society of Japan All Collections About About Authorea FAQs Contact Us Quick Search anywhere Search for preprint articles, keywords, etc. Search Search ADVANCED SEARCH SCROLL This is a preprint and has not been peer reviewed. Data may be preliminary. 18 June 2025 V1 Latest version Share on Transcriptomic and Lipidomic Profiling Reveals Rapid Molecular Responses Associated with Thermotolerance in Chia (Salvia hispanica L.) Leaves Authors : Tannaz Zare 0000-0002-7194-6800 [email protected] , Cheka Kehelpannala , Atul Bhatnagar , Thusitha W. Rupasinghe , Berit Ebert , Alexandre Fournier-Level , and Ute Roessner Authors Info & Affiliations https://doi.org/10.22541/au.175022478.84603687/v1 205 views 136 downloads Contents Abstract Supplementary Material Information & Authors Metrics & Citations View Options References Figures Tables Media Share Abstract Heat stress poses a significant threat to crop productivity; however, the thermotolerance mechanisms in underutilised oilseed crops, such as chia ( Salvia hispanica L.), remain poorly understood. Despite the growing interest in chia as a rich source of ω-3 fatty acids, its molecular response to heat stress, particularly in vegetative tissues, has not been explored. We conducted transcriptomic and lipidomic profiling to examine how chia leaves respond to short-term (3 h) and prolonged (27 h) heat stress (38 °C/20 °C day/night) followed by recovery. Over 20% of transcripts showed differential expression, revealing pathways involving Ca 2+ signalling, heat shock factors, and other stress-related regulators. Almost all heat-responsive genes (>99%) returned to normal levels within 24 hr of recovery, showing a robust restoration of homeostasis. Among 287 annotated lipid species, triacylglycerols exhibited the most significant and reversible changes (>2-fold), suggesting a role in membrane remodelling. These findings highlight the significance of Ca 2+ -mediated signalling pathways and molecular chaperones, such as heat shock proteins and heat shock factors, in heat stress adaptation, providing foundational molecular data on chia leaf thermotolerance. This resource enhances our understanding of stress tolerance mechanisms in chia and contributes to broader efforts in developing heat-resilient oilseed crops. Supplementary Material File (manuscript_zare et al._pcej_2025.docx) Download 4.34 MB Information & Authors Information Version history V1 Version 1 18 June 2025 Copyright This work is licensed under a Non Exclusive No Reuse License. Keywords salvia hispanica calcium signalling heat stress metabalome transcriptome triacylglycerols Authors Affiliations Tannaz Zare 0000-0002-7194-6800 [email protected] The University of Melbourne School of BioSciences View all articles by this author Cheka Kehelpannala Racing Analytical Services Ltd View all articles by this author Atul Bhatnagar The University of Sydney School of Medicine View all articles by this author Thusitha W. Rupasinghe SCIEX View all articles by this author Berit Ebert The University of Melbourne School of BioSciences View all articles by this author Alexandre Fournier-Level The University of Melbourne School of BioSciences View all articles by this author Ute Roessner Australian National University Research School of Biology View all articles by this author Metrics & Citations Metrics Article Usage 205 views 136 downloads .FvxKWukQNSOunydq8rnd { width: 100px; } Citations Download citation Tannaz Zare, Cheka Kehelpannala, Atul Bhatnagar, et al. Transcriptomic and Lipidomic Profiling Reveals Rapid Molecular Responses Associated with Thermotolerance in Chia (Salvia hispanica L.) Leaves. Authorea . 18 June 2025. DOI: https://doi.org/10.22541/au.175022478.84603687/v1 If you have the appropriate software installed, you can download article citation data to the citation manager of your choice. Simply select your manager software from the list below and click Download. For more information or tips please see 'Downloading to a citation manager' in the Help menu . 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