Peculiarities of proteome profile of eutopic endometrium in women with adenomyosis depending on fertility
Observational
OA: green
CC0
AI-generated summary
In women with adenomyosis, infertility correlates with significant eutopic endometrial proteomic alterations in HOXA10, LIF, sgp130, and IL-6 expression compared to fertile patients and healthy controls.
One-sentence paraphrase of the abstract; not a substitute for reading it. No clinical advice. How this works
AI-generated deep summary
This study analyzed the proteomic profile of eutopic endometrium in women with adenomyosis to determine how these molecular changes correlate with fertility status. Researchers conducted comparative immunohistochemical analysis on specimens from infertile patients, fertile patients with a history of successful pregnancies, and healthy controls during the implantation window. The results indicated that infertile women with adenomyosis exhibited significantly decreased expression of HOXA10 and LIF, increased sgp130, and elevated IL-6 secretion compared to both fertile patients and the control group. This paper is centrally about adenomyosis — specifically examining how altered endometrial proteomics associated with the condition contribute to reproductive disorders and infertility.
Read from the paper's body, not the abstract. Not a substitute for reading the paper. No clinical advice. How this works
Abstract
Nosenko O. M., Kosyuga O. M. Peculiarities of proteome profile of eutopic endometrium in women with adenomyosis depending on fertility. Journal of Education, Health and Sport. 2019;9(3):652-662. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.3600575 http://ojs.ukw.edu.pl/index.php/johs/article/view/7666 The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26/01/2017). 1223 Journal of Education, Health and Sport eISSN 2391-8306 7 © The Authors 2019; This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike. (http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. The authors declare that there is no conflict of interests regarding the publication of this paper. Received: 11.03.2019. Revised: 22.03.2019. Accepted: 29.03.2019. PECULIARITIES OF PROTEOME PROFILE OF EUTOPIC ENDOMETRIUM IN WOMEN WITH ADENOMYOSIS DEPENDING ON FERTILITY O. M. Nosenko, O. M. Kosyuga Odessa National Medical University Abstract In recent years, several studies have reported a correlation between adenomyosis and major obstetric adverse effects. Implantation failures can be caused by changes in the expression of some molecules, referred to as "implantation markers", which are expressed by the endometrium and are required for successful interaction between the embryo and endometrium. The purpose of the study was to determine the features of the proteomic profile of the endometrium in women with adenomyosis, depending on their fertility. Material and methods. A comparative immunohistochemical (IGC) analysis of 102 eutopic endometrial specimens from women of reproductive age with adenomyosis and infertility (group I) was performed; from 20 nonpregnant women with adenomyosis who had a history of desired pregnancy and ended in childbirth and did not record episodes of miscarriages (group II), as well as from 30 conditionally gynecologically healthy women who applied for the introduction of an intrauterine system for contraception (control group C). A biopsy of the eutopic endometrium was performed on P+6 day of the menstrual cycle, during the period of the expected implantation window, with the help of aspiration pipel-curette. In immunohistochemical (IGC) determination of the proteomic profile of the endometrium HOXA10 was stained with monoclonal antibodies (MAB) to HOXA10 (sc-17159, Santa Cruz Biotechnology, USA); LIF - murine MAB against LIF (J-14F: SC-80159, Santa Cruz Biotechnology Inc., USA); sgp130 - goat polyclonal antibodies (PAB) anti-gp130 (BAF 228, supplied 50 μg / ml, R&D Systems); IL-6 - primary rabbit antibodies (code No CXH1-066LS, supplied as rabbit IgG 20 µg / ml; Cambridge Bioscience, UK). Results. It was found that important factors of reproductive disorders in women with adenomyosis are: changes in the proteomic profile of the eutopic endometrium on the day of the expected implant window, such as a decrease in HOXA 10 expression in the stroma by 1.52 (p<0.01) and in the glands by 1.46 times (p<0.01), LIF in glands - in 1.12 (p<0.01) and sgp130 - in 2.52 (p<0.01), as well as increased secretion in glands of IL-6 in 1,99 (p<0.01) times. In fertile women with adenomyosis, less pronounced changes in the proteomic profile of the eutopic endometrium are observed, which creates the conditions for successful realization of reproductive intentions, including a decrease in expression of HOXA 10 in the stroma by 1.48 times (p0.05); secretion in glands of LIF - in 1.06 (p>0.05) and gp130 - in 1.98 (p<0.01); increased IL-6 1.08 times (p<0.05). Conclusions. Changes in the proteomic profile of the endometrium play an important role in the occurrence of reproductive disorders in adenomyosis. Key words: adenomyosis; endometrium; infertility; proteomic profile; HOXA10; Leukemia inhibitory factor; glycoprotein 130; interleukin-6.
Full text
4,712 characters
· extracted from
oa-doi-fallback
· click to expand
Peculiarities of proteome profile of eutopic endometrium in women with adenomyosis depending on fertility
Description
Nosenko O. M., Kosyuga O. M. Peculiarities of proteome profile of eutopic endometrium in women with adenomyosis depending on fertility. Journal of Education, Health and Sport. 2019;9(3):652-662. eISSN 2391-8306. DOI http://dx.doi.org/10.5281/zenodo.3600575
http://ojs.ukw.edu.pl/index.php/johs/article/view/7666
The journal has had 7 points in Ministry of Science and Higher Education parametric evaluation. Part B item 1223 (26/01/2017).
1223 Journal of Education, Health and Sport eISSN 2391-8306 7
© The Authors 2019;
This article is published with open access at Licensee Open Journal Systems of Kazimierz Wielki University in Bydgoszcz, Poland
Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author (s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non commercial license Share alike.
(http://creativecommons.org/licenses/by-nc-sa/4.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited.
The authors declare that there is no conflict of interests regarding the publication of this paper.
Received: 11.03.2019. Revised: 22.03.2019. Accepted: 29.03.2019.
PECULIARITIES OF PROTEOME PROFILE OF EUTOPIC ENDOMETRIUM IN WOMEN WITH ADENOMYOSIS
DEPENDING ON FERTILITY
O. M. Nosenko, O. M. Kosyuga
Odessa National Medical University
Abstract
In recent years, several studies have reported a correlation between adenomyosis and major obstetric adverse effects. Implantation failures can be caused by changes in the expression of some molecules, referred to as "implantation markers", which are expressed by the endometrium and are required for successful interaction between the embryo and endometrium. The purpose of the study was to determine the features of the proteomic profile of the endometrium in women with adenomyosis, depending on their fertility. Material and methods. A comparative immunohistochemical (IGC) analysis of 102 eutopic endometrial specimens from women of reproductive age with adenomyosis and infertility (group I) was performed; from 20 nonpregnant women with adenomyosis who had a history of desired pregnancy and ended in childbirth and did not record episodes of miscarriages (group II), as well as from 30 conditionally gynecologically healthy women who applied for the introduction of an intrauterine system for contraception (control group C). A biopsy of the eutopic endometrium was performed on P+6 day of the menstrual cycle, during the period of the expected implantation window, with the help of aspiration pipel-curette. In immunohistochemical (IGC) determination of the proteomic profile of the endometrium HOXA10 was stained with monoclonal antibodies (MAB) to HOXA10 (sc-17159, Santa Cruz Biotechnology, USA); LIF - murine MAB against LIF (J-14F: SC-80159, Santa Cruz Biotechnology Inc., USA); sgp130 - goat polyclonal antibodies (PAB) anti-gp130 (BAF 228, supplied 50 μg / ml, R&D Systems); IL-6 - primary rabbit antibodies (code No CXH1-066LS, supplied as rabbit IgG 20 µg / ml; Cambridge Bioscience, UK). Results. It was found that important factors of reproductive disorders in women with adenomyosis are: changes in the proteomic profile of the eutopic endometrium on the day of the expected implant window, such as a decrease in HOXA 10 expression in the stroma by 1.52 (p<0.01) and in the glands by 1.46 times (p<0.01), LIF in glands - in 1.12 (p<0.01) and sgp130 - in 2.52 (p<0.01), as well as increased secretion in glands of IL-6 in 1,99 (p<0.01) times. In fertile women with adenomyosis, less pronounced changes in the proteomic profile of the eutopic endometrium are observed, which creates the conditions for successful realization of reproductive intentions, including a decrease in expression of HOXA 10 in the stroma by 1.48 times (p0.05); secretion in glands of LIF - in 1.06 (p>0.05) and gp130 - in 1.98 (p<0.01); increased IL-6 1.08 times (p<0.05). Conclusions. Changes in the proteomic profile of the endometrium play an important role in the occurrence of reproductive disorders in adenomyosis.
Key words: adenomyosis; endometrium; infertility; proteomic profile; HOXA10; Leukemia inhibitory factor; glycoprotein 130; interleukin-6.
Files
7666.pdf
Files
(878.3 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:39cd0f6b6dd625ee7875b974e6951fe3
|
878.3 kB | Preview Download |
Text is read by the "Ask this paper" AI Q&A widget below. Extraction quality varies by source — PMC NXML preserves structure cleanly, OA-HTML may include some navigation residue, and OA-PDF can have broken hyphenation. The publisher copy (via DOI) is the canonical version.
My notes (saved in your browser only)
Ask this paper
Answers must be backed by verbatim quotes from this paper's full text. Hallucinated quotes are dropped automatically; if no verbatim passage answers the question, we say so. How this works
Condition tags
Citation neighborhood (no data yet)
We don't have any in-corpus citations linked to this paper yet. The paper's references may be in our DB but unresolved to ``paper_id`` (resolution happens at ingest when the cited DOI matches a row we already have). Run the cross-source citation reconcile pass to retry.
Source provenance
- openalex
- last seen: 2026-05-10T10:38:40.447481+00:00
License: CC0
· commercial use OK