The role of Guizhifulingwan on angiogenesis of endometriosis model rat

Aim: To investigate the role of Guizhifulingwan(GFW) on angiogenesis factor and microvessel density(MVD) of endometriosis(EM) model rat. Methods: EM rats were treated with GFW, Danazol(D), and synergic treatment(S) of GFW and D respectively for 4 weeks. The histomorphology and volumes of ectopic endometrium were observed, The amount of macrophages in peritoneal fluid of EM model rats was counted, the concentration of interleukin-8(IL-8)and tumor necrosis factor-α(TNF-α)of EM model rats in serum, peritoneal fluid, and supernate of cultured macrophages were measured, vascular endothelial growth factor(VEGF) was detected in ectopic endometrium by immunohistochemical SABC technique, MVD was determined by immunostaining for CD34. Similar studies were performed in group of rats without treatment(U)and another group with sham operation(C). Results: Compared with U group, GFW group, D group, and S group displayed an significant atrophy of ectopic endometrium, reduced number of endometrial glands, decreased macrophage amounts and low concentrations of IL-8 and TNF-α in blood, peritoneal fluid, and supernate of cultured macrophages. The expression of VEGF and MVD of ectopic endometrium in U group were significantly higher than that of C group. After treatment, they all decreased significantly, especially in S group. Conclusion:Both GFW and danazol can affect angiogenesis of EM model rats, cause an obvious atrophy in ectopic endometrium. The effect could be enhanced when they are used synergically.