Novel peptide design and evaluation against actin and α5β1integrin

Novel peptide design and evaluation against actin and α5β1integrin | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Short Report Novel peptide design and evaluation against actin and α5β1integrin Sasidhar Reddy Eda, Sivaranjani Pakala, Ramesh Ummanni, Umamaheswari Amineni, and 1 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-4003076/v1 This work is licensed under a CC BY 4.0 License Status: Posted Version 1 posted You are reading this latest preprint version Abstract Adhesion-dependent cells rely on actin and α5β1 integrin interactions to establish the cytoskeleton, signaling their survival and growth. We investigated the persistence of the complex in early transformed rat fibroblast cells, which opens the possibility of halting tumor growth by inhibiting the formation of the actin and α5β1 integrin complex. Hence, actin and α5β1 integrin were targeted for developing novel peptides to disrupt their association and inhibit tumor growth. Seventeen heptapeptides were designed based on actin- α5β1 integrin complex by using in silico techniques such as peptide designing, homology modeling, docking, molecular dynamics simulations, and in vitro methods using a cellular tumor model system to study their viability using MTT assay. Docking was performed for actin, α5β1 integrin with existing peptide inhibitor ATN 161, designed peptides and small molecule inhibitor Cytochalasin D using Schrodinger. Docking revealed that actin and α5β1 integrin had higher affinity for sixteen and five peptides respectively. TRFKKGY, the top ranked peptide of actin and α5β1 integrin was cytotoxic to transformed rat fibroblast cells grown in non-adherent conditions. This data suggests that the peptide designed has a minimal inhibitory effect on cell growth. However, it showed maximum cell death in transformed rat fibroblast F111 cells. Full Text Additional Declarations No competing interests reported. Cite Share Download PDF Status: Posted Version 1 posted You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-4003076","acceptedTermsAndConditions":true,"allowDirectSubmit":true,"archivedVersions":[],"articleType":"Short Report","associatedPublications":[],"authors":[{"id":278613482,"identity":"aa7c8a32-7d6c-4ea2-a419-298ce5ae06fc","order_by":0,"name":"Sasidhar Reddy Eda","email":"","orcid":"","institution":"Acharya Nagarjuna University","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Sasidhar","middleName":"Reddy","lastName":"Eda","suffix":""},{"id":278613483,"identity":"10b59269-715c-45b9-b378-58c24f0d30f1","order_by":1,"name":"Sivaranjani Pakala","email":"","orcid":"","institution":"Sri Venkateswara Institute of Medical Sciences","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Sivaranjani","middleName":"","lastName":"Pakala","suffix":""},{"id":278613484,"identity":"1c6e94b2-eede-422c-a5a4-8d99cedd1018","order_by":2,"name":"Ramesh Ummanni","email":"","orcid":"","institution":"CSIR- IICT","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Ramesh","middleName":"","lastName":"Ummanni","suffix":""},{"id":278613485,"identity":"e9a6854e-1647-4b69-b98c-9d488b18ddd5","order_by":3,"name":"Umamaheswari Amineni","email":"","orcid":"","institution":"Sri Venkateswara Institute of Medical Sciences","correspondingAuthor":false,"submittingAuthor":false,"prefix":"","firstName":"Umamaheswari","middleName":"","lastName":"Amineni","suffix":""},{"id":278613487,"identity":"f5473d82-438c-41cc-9214-782aa7909711","order_by":4,"name":"Rajeswari Jinka","email":"data:image/png;base64,iVBORw0KGgoAAAANSUhEUgAAAZAAAAAyAQMAAABI0h/eAAAABlBMVEX///8AAABVwtN+AAAACXBIWXMAAA7EAAAOxAGVKw4bAAAA+klEQVRIiWNgGAWjYBACxmYGZhDNDOEa2IDEGg+QoiUNJNaAVwtCNQQcBpN4tTC38x42+LnHjp2f/4zh54qC83Zr2w8DbamxicbtML7kxJ5nycySM3KMJc8Y3E7ediYRqOVYWm4DTi08xgd4DjAzG9zg3SDZANRidgCohbHhMF4tB/8cqGe2P392888Gg3PJZucfEtaSzHPgMLMBQ+42oC0H7MxuEGGLscyB48wSN/K/WTYYJCeY3QDakoDHL4b9Z4wl3xyoTubvP5Z8s+GPnb3Z+fSHDz7U2ODWApVIhgkkggUScCgHAXkobQcTsMejeBSMglEwCkYoAAAP3WAN6ZYdOgAAAABJRU5ErkJggg==","orcid":"","institution":"Acharya Nagarjuna University","correspondingAuthor":true,"submittingAuthor":false,"prefix":"","firstName":"Rajeswari","middleName":"","lastName":"Jinka","suffix":""}],"badges":[],"createdAt":"2024-03-01 11:50:50","currentVersionCode":1,"declarations":"","doi":"10.21203/rs.3.rs-4003076/v1","doiUrl":"https://doi.org/10.21203/rs.3.rs-4003076/v1","draftVersion":[],"editorialEvents":[],"editorialNote":"","failedWorkflow":false,"files":[{"id":58727271,"identity":"ffaf5670-2375-42d7-90c7-6f5339d0d941","added_by":"auto","created_at":"2024-06-20 10:16:23","extension":"pdf","order_by":1,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1169056,"visible":true,"origin":"","legend":"","description":"","filename":"Manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-4003076/v1_covered_28bb781a-d45b-46bd-b146-d648a0f18a46.pdf"}],"financialInterests":"No competing interests reported.","formattedTitle":"Novel peptide design and evaluation against actin and α5β1integrin","fulltext":[],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":false,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":true,"highlight":"","institution":"","isAcceptedByJournal":false,"isAuthorSuppliedPdf":true,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":false,"isPdf":true,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"researchsquare","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":true,"externalIdentity":"","sideBox":"","snPcode":"","submissionUrl":"/submission","title":"Research Square","twitterHandle":"researchsquare","acdcEnabled":true,"dfaEnabled":false,"editorialSystem":"","reportingPortfolio":"","inReviewEnabled":false,"inReviewRevisionsEnabled":true},"keywords":"","lastPublishedDoi":"10.21203/rs.3.rs-4003076/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-4003076/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"Adhesion-dependent cells rely on actin and α5β1 integrin interactions to establish the cytoskeleton, signaling their survival and growth. 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