Morphometric and molecular identification of Unionicola chelata (Acari: Hydrachnida: Unionicolidae) from Bellamya bengalensis, a gastropod mollusc in South 24 Parganas district, West Bengal, India

Morphometric and molecular identification of Unionicola chelata (Acari: Hydrachnida: Unionicolidae) from Bellamya bengalensis, a gastropod mollusc in South 24 Parganas district, West Bengal, India | Research Square window.SnipcartSettings = { analytics: { enabled: false } }; (function() { var accessVector = localStorage.getItem('access_vector') || ''; window.dataLayer = window.dataLayer || []; if (accessVector) { window.dataLayer.push({ user: { profile: { profileInfo: { snid: accessVector } } } }); } })(); (function(w,d,s,l,i){w[l]=w[l]||[];w[l].push({'gtm.start':new Date().getTime(),event:'gtm.js'});var f=d.getElementsByTagName(s)[0],j=d.createElement(s),dl=l!='dataLayer'?'&l='+l:'';j.async=true;j.src='https://www.googletagmanager.com/gtm.js?id='+i+dl;f.parentNode.insertBefore(j,f);})(window,document,'script','dataLayer','GTM-K279D39R'); Browse Preprints In Review Journals COVID-19 Preprints AJE Video Bytes Research Tools Research Promotion AJE Professional Editing AJE Rubriq About Preprint Platform In Review Editorial Policies Our Team Advisory Board Help Center Sign In Submit a Preprint Cite Share Download PDF Research Article Morphometric and molecular identification of Unionicola chelata (Acari: Hydrachnida: Unionicolidae) from Bellamya bengalensis, a gastropod mollusc in South 24 Parganas district, West Bengal, India Biplab Bhowmik, Bipasa Dey, Shantanabha Das, Gyan Deb Barman, and 2 more This is a preprint; it has not been peer reviewed by a journal. https://doi.org/ 10.21203/rs.3.rs-3945341/v1 This work is licensed under a CC BY 4.0 License Status: Published Journal Publication published 26 Jul, 2024 Read the published version in Journal of Parasitic Diseases → Version 1 posted 5 You are reading this latest preprint version Abstract Unionicola sp. is a parasitic aquatic mite known to infect freshwater aquatic organisms, especially the malacofauna species and few species of sponges. Unionicola chelata (Acari: Hydrachnida: Unionicolidae) generally infect the freshwater bivalves of the Genus Unio sp. They are usually facultative in nature and can be parasitic at any stage of their life cycle. They cause damage to the gills of the host which harms their normal respiration process. The present work portraits the morphological characters, morphometric details, Scanning Electron Microscopy (SEM) images and sequence data of its mitochondrial gene “Cytochrome Oxidase I (COI)” and its taxonomic position was justified by obtaining a phylogenetic tree. The description regarding its morphological, morphometric characters and sequence studies has been presented here. This paper holds the report of a parasitic aquatic mite Unionicola chelata for the first time from a Gastropod molluscan host Bellamya bengalensis (Lamarck, 1882), from Diamond Harbour, South 24 Parganas, West Bengal, India. Bellamya bengalensis Unionicola chelata Morphological characters SEM Gene Sequence Figures Figure 1 Figure 2 Figure 3 Introduction Mollusca, the second largest invertebrate Phylum of the animal kingdom after Phylum Arthropoda that holds more than 1,00,000 species (Rosenberg, 1996) including terrestrial, freshwater and marine forms. Malacofauna is mainly characterized by the presence of mantle that encloses its internal organs. ‘Radula’, a scraping organ is used for feeding purposes. They consist of seven classes namely: Aplacophora, Monoplacophora, Polyplacophora, Cephalopoda, Gastropoda, Scaphopoda and Bivalvia. The freshwater species of malacofauna generally belong to the classes of Gastropoda and Bivalvia. Many freshwater molluscan species are economically beneficial to human beings. They are a rich source of protein, carbohydrates and several other nutrients while low in cholesterol and fats (Moniruzzaman et al, 2021) and are therefore consumed in many nations like India (Rao-Subba and Dey, 1989); Mexico (Gracia- Cubas 1963); Taiwan, Formosa and Thailand (Baby et al, 2010). They can also help in detecting the quality of their environment, especially in detecting the heavy metals present in water (Naimo TJ 1995). Many malacofauna species are often reported to be infected with different parasites, which include protozoa, ciliates, helminthes, mites etc. Two Genus of aquatic mites: Najadicola sp. and Unionicola sp. are known to infect different species of freshwater malacofauna (Chapurina et al, 2022a Chapurina et al; 2022b). The Genus of Unionicola have been reported with 58 subgenera and above 260 species (Chapurina 2021, Edwards and Vidrine 2013, Savatenalinton 2017); while more species are still being recorded from different geographical area with minute differences in morphological features and genotype (Chapurina 2021, Lewisch 2021, Smith 2017, Ding 2019). They are facultative parasites and infect the molluscan host during a particular stage of their life. Their life cycle consists of egg, larvae, nymphocrysalis, nymph, teleocrysalis and adult. They can be parasitic either during one or few or all stages of their life cycle (Mitchell 1995, Bottger 1997, Simmons and Smith1948, Edwards and Virdine 2013). They cause the gills of their host to get covered by slimy threads which disturb their proper functioning. In some cases, they also cause mild lesions in the infected areas (Guo and Ford 2016).Therefore, proper identification and classifications of these mites are important in order to mitigate their effects. The mites belonging to the Unionicolidae family mostly infect the freshwater mussels belonging to the Genus Unio sp. They are also found to infect many other species of freshwater bivalves and sponges (Davis 1973 and Wen et al., 2006). The mites of the Genus Unionicola sp. infect different tissues of the host, such as gills, foot and mantle (Edwards and Virdine 2006). Some genera belonging to this family are also ectoparasitic and prefer to remain at the surface of the host. They lay their eggs on the gill flaps, mantle or body wall (Wen et al., 2006). Some species also encyst into the foot and mantle of their host. U. abnormipens can also get burrowed beneath the epidermis (Faust 1924; Mitchell and Gunter 2006). U. ypsilophora embeds into the gills of the unionid bivalves and cause them to get covered with slimy mucus threads reducing their firmness (Davis 1973).Baker and Mann 1997 noticed lesions caused by mites of the Genus Unionicola sp. there was swelling in the gills and increase in the amount of localized haemocytes (Baker and Mann 1977). At present, there are 209 species of Unionid mites distributed around the globe belonging to eight subgenera (Virdine 2008). The different subgenera or genus of these mites differ from only one or two morphological features. Moreover, they have high productivity, fast developmental process and also short life cycle. This helps them to have a better host-parasite relation and also promotes co-evolution along with their host. This leads to rapid morphological modifications in the mites (Ernsting 2008). Therefore, sequence analysis can prove to be the best and most appropriate method to identify the parasitic aquatic mites. The identification of the mentioned aquatic parasitic mite in this paper has been justified by morphometric data, SEM images and DNA bar-coding results using the COI gene based molecular methods. And this present work signifies it with morphological, morphometric, Scanning Electron Microscopy and Gene sequencing methods. Materials and methods In the following research paper, freshwater gastropod mollusk belonging to the Genus Bellamya sp. was studied for the presence of parasitic aquatic mites. Collection of species and light microscopic study Adult specimens of Bellamya sp. was collected from different freshwater bodies in Diamond Harbour, South 24 Parganas from August 2021- August 2023. They were brought to the lab and dissected. The gill of the host was seen to be infected with the aquatic mites. Some mites were directly put in slide and studied under Olympus BX43 compound microscope for morphology. The measurement was done using CARL ZEISS trinocular stereo zoom microscope MODEL: Stemi 508, SN: 3951006519. Scanning Electron Microscopic Studies The mites were fixed in 2.5% glutaraldehyde solution for overnight. Then they were washed in cacodylate buffer and dehydrated in a graded series of alcohol. Then the samples were infiltrated with amyl acetate and dried to critical point and coated with gold and observed for Scanning Electron Microscopy (SEM) using Carl Zeiss Scanning Electron Microscope MODEL: Gemini; Sigma 300 at 500 (Kilo Volt) KV. Sequencing Analysis For molecular identification, the DNA of the sample was isolated. The quality of the DNA was evaluated with 1.0% agarose gel. The COI gene was then isolated and amplified using HCO and LCO primers. Resolution was done on agarose gel where a single discrete amplicon band of 700 bp was obtained. Any contamination was removed by purification of the band. The PCR amplicon was subjected to forward and reverse sequencing studies on an ABI 3730xl Genetic Analyzer using a BDT v3.1 Cycle sequencing kit. The consensus sequence was generated with the help of aligner software. BLAST was carried out using the COI gene sequence with the ‘nr’ database of the NCBI gene bank database. First ten sequence based on their maximum identity scores were selected and then aligned by using the multiple alignment software program Clustal W. RPD database was used to generate the distance matrix. A phylogenetic tree was constructed using MEGA 11. Results Morphological description Dorsoventrally flattened, oval shaped body. Body divided into head, thorax and abdomen. Greenish-black in colour. The carapace is sclerotised and covers the idiostoma. Feeding appendages consist of chelicerae and pedipalp which are attached to the capitulum. Opening of the genital pore is highly sclerotised which is also known as the genital plate. Setae cover the genital plate. Presence of four pairs of jointed appendages. Location of the genital field at the post-ventor part of the body. Morphometric measurements were – (DP) -±443µm, Idiostoma (IS) - ±467µm, Capitulum (CA)- ± , Pedipalp (PP) - ±95µm, 1 st pair of leg (I-L) - ± 380 µm, 2 nd pair of leg (II-L)- ± 437 µm, 3 rd pair of leg (III-L) - ± 490 µm, 4 th pair of leg (IV-L) - ± 631 µm, Carapace (C) - ± 457 µm Taxonomic Summary Type Material: Unionicola chelata Host : Bellamya bengalensis Study Site: Diamond Harbour, South 24 Parganas district, West Bengal Locality: Nabasan, West Bengal, India 754R+377, Nabasan, Sarisha, West Bengal 743368 Latitude: 22.255164 o North Longitude: 88.19123 o East Site of Infection: Gill Total number of samples observed: 37 Number of infected samples: 23 Prevalence: 23/37 (62.162%) Symbiotype: BDW/03 is deposited in the museum of Department of Zoology, Diamond Harbour Women’s University, Sarisha, Diamond Harbour 743368, West Bengal, India Holotype: UC/08 is deposited in the museum of Department of Zoology, Diamond Harbour Women’s University, Sarisha, Diamond Harbour 743368, West Bengal, India Paratype: UC/04, UC/05 and other slides are deposited in the collection of the Parasitology Laboratory, Department of Zoology, Diamond Harbour Women’s University, Sarisha, Diamond Harbour 743368, West Bengal, India The scanning electron microscopic images revealed the dorsal side of the mite, showing the Capitulum (CA), Walking Legs (WL), and the Dorsal Plate (DP), a pair of Pedipalp (PP) and a prominent Genital Ridge (GR). The walking legs showed the presence of bristles. The following figures 2(a-c) are obtained from the Scanning Electron Microscopic study of the mite. Sequencing results Distance matrix The distance matrix was calculated in order to find out the base substitution between the sequences per site. The “Maximum Composite Likelihood Model” was conducted for analysis (Kimura et al., 1980). This involved about 11 nucleotide sequences. 1 st , 2 nd , 3 rd and non-coding positions were included. A total of 775 positions were placed in the final database. Evolutionary analysis was done using MEGA-11(Kumar et al., 2018). The following two tables Table I and Table II were obtained from the sequence studies of the mite. Table I depicts the evolutionary divergence between the sequences and Table II lists the sequences producing similar alignments. Table I: Estimates of the Evolutionary Divergence between sequence Table II: Sequences producing similar alignments Phylogenetic Tree The evolutionary relationship was derived by using the Tamura-Nei model and Maximum likelihood method (Kimura et al., 1980). Initial phylogenetic trees were obtained for the heuristic search by the application of Bio NJ algorithm Neighbour – Join to a parirwise distance matrix and then by selecting the topology of the superior log. Total 11 nucleotide sequences were analyzed including the codon positions of 1 st +2 nd +3 rd non-coding regions. A total of 775 positions were there in the final dataset. The evolutionary relationship was analysed by the MEGA 11 software (Kumar et al., 2018). The following table presents a comparison of morphological features between Unionicola chelata , described in this paper and the previously discovered similar species of parasitic aquatic mites. Table III: Comparison of the morphological features of U.chelata and similar species Discussion The present paper deals with three different methods for identification of an aquatic mite collected from freshwater gastropod mollusc Bellamya bengalensis. These identification methods included morphological studies, Scanning Electron Microscopic studies and Sequencing. The morphological characters observed using compound microscopy showed differences in some features from the previously identified similar species of mites belonging to the same Genus which is presented in a comparative table. The SEM images shows a clear view of the dorsal side of the mite, its walking leg and a prominent genital ridge. These features also indicate that the species is quite different than the other mentioned species of the Genus Unionicola while its features match to that of Unionicola chelata. The gene sequencing studies are done using the MT COI gene. It has been used to generate a distance matrix and a phylogenetic tree which has revealed its taxonomic position. Unionicola chelata has been reported from unionid bivalves so far. In this paper, new geographical area and new host species of the parasitic arthropod has been reported. The morphometric, microscopic and sequencing results have confirmed the species to be Unionicola chelata . This mite has been reported for the first time from a new host Bellamya bengalensis from South 24 Parganas district, West Bengal. Before, Unionicola savadiensis was reported from India in freshwater bivalve mussels but Unionicola chelata have been reported Bellamya sp. for the first time from West Bengal, India. Declarations Acknowledgement The authors are grateful to the Department of Zoology, Diamond Harbour Women’s University for granting us the permission to carry out this research work. The authors are grateful to the Department of Zoology, Diamond Harbour Women’s University for granting us the permission to carry out this research work. We extend our thanks to Burdhwan University, for letting us perform the Scanning Electron Microscopic studies in their lab. We are also thankful to Barcode Biosciences, Bangalore for the sequence analysis. Conflict of Interest The authors of this paper have no conflict of interest regarding the commercial, academic, political, financial and personal aspects. Authors Contributions All the authors have equally contributed to the successful writing of this paper and the works associated with it. Funding No amount of fund was received during for carrying out any field or lab based work. Competing Interests The authors have no competing interests References Baby RL, Hasan I, Kabir KA, Naser MN (2010) Nutrient analysis of some commercially important mollusks of Bangladesh. J. Sci. Res. 2(@): 390-396 Baker P, Mann R (1997). 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Tables Table I: Estimates of the Evolutionary Divergence between sequence SAMPLE_B 4.655 4.658 4.653 0.864 0.862 0.862 0.862 0.862 0.857 0.787 FJ218009.1 1.037 0.004 0.006 4.910 3.837 3.837 3.837 3.837 3.729 4.214 FJ218006.1 1.051 0.008 0.005 4.912 3.840 3.840 3.840 3.840 3.732 4.216 FJ218018.1 1.047 0.015 0.014 4.919 3.850 3.850 3.850 3.850 3.743 4.215 MT724648.1 0.245 1.121 1.135 1.130 0.809 0.809 0.809 0.809 0.794 0.990 MN360249.1 0.255 0.840 0.853 0.853 0.219 0.000 0.000 0.000 0.000 0.752 MN358857.1 0.255 0.840 0.853 0.853 0.219 0.000 0.000 0.000 0.752 MN358526.1 0.255 0.840 0.853 0.853 0.219 0.000 0.000 0.000 0.000 0.000 0.752 MN362553.1 0.255 0.840 0.853 0.853 0.219 0.000 0.000 0.000 0.000 0.752 KR099587.1 0.252 0.828 0.841 0.841 0.215 0.000 0.000 0.000 0.000 0.746 MG317695.1 0.265 0.960 0.973 0.966 0.265 0.227 0.227 0.227 0.227 0.226 Table II: Sequences producing similar alignments Description Max Score Total Score Query Cover E value Per. Ident Accession Unionicola chelata isolate 17 627 627 100% 1e-174 83.87% FJ218009.1 Unionicola chelata isolate 33 614 614 99% 1e-170 83.64% FJ218006.1 Unionicola chelata isolate 78 610 610 100% 1e-169 83.41% FJ218018.1 Unionicola ( Iridinicola ) sp. Hyd_541_1 534 534 99% 8e-147 81.53% MT724648.1 Unionicola sp. BOLD:ACG4692 BIOUG33431-A08 505 505 99% 7e-138 80.67% MN360249.1 Unionicola sp. BOLD:ACG4692 BIOUG33431-A11 505 505 99% 7e-138 80.67% MN358857.1 Unionicola sp. BOLD:ACG4692 BIOUG33431-A09 505 505 99% 7e-138 80.67% MN358526.1 Unionicola sp. BOLD:ACG4692 BIOUG33431-A05 505 505 99% 7e-138 80.67% MN362553.1 Unionicola sp. BOLD:ACG4692 BIOUG11770-E10 497 497 96% 1e-135 80.82% KR099587.1 Neumania sp. BIOUG27038-D03 486 486 98% 2e-132 80.37% MG317695.1 Table III: Comparison of the morphological features of U.chelata and similar species S. No. Infecting Species Host Site of Infection Morphological features 1. U. haungthaya Lamellidens generous Gill, Mantle Dorsum lacking dorsal patelets Presence of distinct margins in the coxal plate Female genital field (31-50) acetabula Male genital plate(22-33)acetabula Stocky and well sclerotised pedipalp 2. U. savadiensis L.savadienss and L.generous Gill, Mantle Without dorsal platelets Without apperent posterior margin (13-20) both male and female 3. U. generosa Lamellidens generous Gill, Mantle Lacks dorsal platelets 15-18 acetabula in males 4. U.chelata B. bengalensis Gill, Mantle carapace is sclerotised pedipalp attached to the capitulum Opening of genital pore highly sclerotised Cite Share Download PDF Status: Published Journal Publication published 26 Jul, 2024 Read the published version in Journal of Parasitic Diseases → Version 1 posted Editorial decision: Major revisions needed 02 Jun, 2024 Reviewers agreed at journal 25 Apr, 2024 Reviewers invited by journal 22 Apr, 2024 Editor assigned by journal 09 Feb, 2024 First submitted to journal 08 Feb, 2024 You are reading this latest preprint version Research Square lets you share your work early, gain feedback from the community, and start making changes to your manuscript prior to peer review in a journal. As a division of Research Square Company, we’re committed to making research communication faster, fairer, and more useful. We do this by developing innovative software and high quality services for the global research community. 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Also discoverable on Platform About Our Team In Review Editorial Policies Advisory Board Help Center Resources Author Services Accessibility API Access RSS feed Manage Cookie Preferences © Research Square 2026 | ISSN 2693-5015 (online) Privacy Policy Terms of Service Do Not Sell My Personal Information {"props":{"pageProps":{"initialData":{"identity":"rs-3945341","acceptedTermsAndConditions":true,"allowDirectSubmit":false,"archivedVersions":[],"articleType":"Research Article","associatedPublications":[],"authors":[{"id":293844750,"identity":"d42e6b65-9e69-45f4-b3d8-1193d800e046","order_by":0,"name":"Biplab 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1","display":"","copyAsset":false,"role":"figure","size":383491,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003e(a-d): Compound Microscopic Images of \u003c/strong\u003e\u003cem\u003e\u003cstrong\u003eUnionicola chelata\u003c/strong\u003e\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e(a) Site of Collection\u003c/p\u003e\n\u003cp\u003e(b) Collected Host Specimen\u003c/p\u003e\n\u003cp\u003e(c) Compound Microscopic Image of \u003cem\u003eUnionicola chelata\u003c/em\u003e\u003c/p\u003e","description":"","filename":"1.jpg","url":"https://assets-eu.researchsquare.com/files/rs-3945341/v1/128a55089507e56378c315c0.jpg"},{"id":55405449,"identity":"eb25ae3b-6ac9-4517-9a63-e087470914e6","added_by":"auto","created_at":"2024-04-26 20:41:36","extension":"jpg","order_by":2,"title":"Figure 2","display":"","copyAsset":false,"role":"figure","size":306881,"visible":true,"origin":"","legend":"\u003cp\u003e\u003cstrong\u003e(a-c): Scanning Electron\u003c/strong\u003e \u003cstrong\u003eMicroscopic Images of \u003c/strong\u003e\u003cem\u003e\u003cstrong\u003eUnionicola chelata\u003c/strong\u003e\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e(a): SEM images of \u003cem\u003eU.chelata\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e(b): Walking Leg of the Arthropod\u003c/p\u003e\n\u003cp\u003e(c): Genital Ridge\u003c/p\u003e","description":"","filename":"2.jpg","url":"https://assets-eu.researchsquare.com/files/rs-3945341/v1/b53e15e2e2592408f461a435.jpg"},{"id":55405012,"identity":"1b06f359-210e-4af5-82c9-a48d7798ff57","added_by":"auto","created_at":"2024-04-26 20:33:36","extension":"jpg","order_by":3,"title":"Figure 3","display":"","copyAsset":false,"role":"figure","size":110099,"visible":true,"origin":"","legend":"\u003cp\u003ePhylogenetic analysis of the studied aquatic arthropod \u003cem\u003eUnionicola chelata\u003c/em\u003e based on MT COI gene with maximum likelihood algorithm.\u003c/p\u003e","description":"","filename":"3.jpg","url":"https://assets-eu.researchsquare.com/files/rs-3945341/v1/c5424003cc1241a69faf41ba.jpg"},{"id":61596156,"identity":"67024930-ab7d-41ef-a44a-3e40b0f054dd","added_by":"auto","created_at":"2024-08-01 17:25:12","extension":"pdf","order_by":0,"title":"","display":"","copyAsset":false,"role":"manuscript-pdf","size":1584304,"visible":true,"origin":"","legend":"","description":"","filename":"manuscript.pdf","url":"https://assets-eu.researchsquare.com/files/rs-3945341/v1/11fbca6c-d11d-4d0d-8158-813661bebcb0.pdf"}],"financialInterests":"","formattedTitle":"Morphometric and molecular identification of Unionicola chelata (Acari: Hydrachnida: Unionicolidae) from Bellamya bengalensis, a gastropod mollusc in South 24 Parganas district, West Bengal, India","fulltext":[{"header":"Introduction","content":"\u003cp\u003eMollusca, the second largest invertebrate Phylum of the animal kingdom after Phylum Arthropoda that holds more than 1,00,000 species (Rosenberg, 1996) including terrestrial, freshwater and marine forms. Malacofauna is mainly characterized by the presence of mantle that encloses its internal organs. \u0026lsquo;Radula\u0026rsquo;, a scraping organ is used for feeding purposes. They consist of seven classes namely: Aplacophora, Monoplacophora, Polyplacophora, Cephalopoda, Gastropoda, Scaphopoda and Bivalvia. The freshwater species of malacofauna generally belong to the classes of Gastropoda and Bivalvia. Many freshwater molluscan species are economically beneficial to human beings. They are a rich source of protein, carbohydrates and several other nutrients while low in cholesterol and fats (Moniruzzaman et al, 2021) and are therefore consumed in many nations like India (Rao-Subba and Dey, 1989); Mexico (Gracia- Cubas 1963); Taiwan, Formosa and Thailand (Baby et al, 2010). They can also help in detecting the quality of their environment, especially in detecting the heavy metals present in water (Naimo TJ 1995).\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eMany malacofauna species are often reported to be infected with different parasites, which include protozoa, ciliates, helminthes, mites etc. Two Genus of aquatic mites: \u003cem\u003eNajadicola\u0026nbsp;\u003c/em\u003esp. and \u003cem\u003eUnionicola\u0026nbsp;\u003c/em\u003esp. are known to infect different species of freshwater malacofauna (Chapurina et al, 2022a Chapurina et al; 2022b). The Genus of Unionicola have been reported with 58 subgenera and above 260 species (Chapurina 2021, Edwards and Vidrine 2013, Savatenalinton\u0026nbsp;2017); while more species are still being recorded from different geographical area with minute differences in morphological features and genotype (Chapurina 2021, Lewisch 2021, Smith 2017, Ding 2019). They are facultative parasites and infect the molluscan host during a particular stage of their life. Their life cycle consists of egg, larvae, nymphocrysalis, nymph, teleocrysalis and adult. They can be parasitic either during one or few or all stages of their life cycle (Mitchell 1995, Bottger 1997, Simmons and Smith1948, Edwards and Virdine 2013). They cause the gills of their host to get covered by slimy threads which disturb their proper functioning. In some cases, they also cause mild lesions in the infected areas (Guo and Ford 2016).Therefore, proper identification and classifications of these mites are important in order to mitigate their effects.\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe mites belonging to the Unionicolidae family mostly infect the freshwater mussels belonging to the Genus \u003cem\u003eUnio\u0026nbsp;\u003c/em\u003esp. They are also found to infect many other species of freshwater bivalves and sponges (Davis 1973 and Wen et al., 2006). The mites of the Genus \u003cem\u003eUnionicola\u0026nbsp;\u003c/em\u003esp. infect different tissues of the host, such as gills, foot and mantle (Edwards and Virdine 2006). Some genera belonging to this family are also ectoparasitic and prefer to remain at the surface of the host. They lay their eggs on the gill flaps, mantle or body wall (Wen et al., 2006). Some species also encyst into the foot and mantle of their host. \u003cem\u003eU. abnormipens\u0026nbsp;\u003c/em\u003ecan also get burrowed beneath the epidermis (Faust 1924; Mitchell and Gunter 2006). \u003cem\u003eU. ypsilophora\u003c/em\u003e embeds into the gills of the unionid bivalves and cause them to get covered with slimy mucus threads reducing their firmness (Davis 1973).Baker and Mann 1997 noticed lesions caused by mites of the Genus \u003cem\u003eUnionicola\u0026nbsp;\u003c/em\u003esp. there was swelling in the gills and increase in the amount of localized haemocytes (Baker and Mann 1977).\u003c/p\u003e\n\u003cp\u003eAt present, there are 209 species of Unionid mites distributed around the globe belonging to eight subgenera (Virdine 2008). The different subgenera or genus of these mites differ from only one or two morphological features. Moreover, they have high productivity, fast developmental process and also short life cycle. This helps them to have a better host-parasite relation and also promotes co-evolution along with their host. This leads to rapid morphological modifications in the mites (Ernsting 2008). Therefore, sequence analysis can prove to be the best and most appropriate method to identify the parasitic aquatic mites. \u0026nbsp;\u0026nbsp;\u003c/p\u003e\n\u003cp\u003eThe identification of the mentioned aquatic parasitic mite in this paper has been justified by morphometric data, SEM images and DNA bar-coding results using the COI gene based molecular methods. And this present work signifies it with morphological, morphometric, Scanning Electron Microscopy and Gene sequencing methods.\u003c/p\u003e"},{"header":"Materials and methods","content":"\u003cp\u003eIn the following research paper, freshwater gastropod mollusk belonging to the Genus \u003cem\u003eBellamya\u003c/em\u003e sp. was studied for the presence of parasitic aquatic mites.\u003c/p\u003e \u003cdiv id=\"Sec2\" class=\"Section2\"\u003e \u003ch2\u003eCollection of species and light microscopic study\u003c/h2\u003e \u003cp\u003eAdult specimens of \u003cem\u003eBellamya\u003c/em\u003e sp. was collected from different freshwater bodies in Diamond Harbour, South 24 Parganas from August 2021- August 2023. They were brought to the lab and dissected. The gill of the host was seen to be infected with the aquatic mites. Some mites were directly put in slide and studied under Olympus BX43 compound microscope for morphology. The measurement was done using CARL ZEISS trinocular stereo zoom microscope MODEL: Stemi 508, SN: 3951006519.\u003c/p\u003e \u003c/div\u003e \u003cdiv id=\"Sec3\" class=\"Section2\"\u003e \u003ch2\u003eScanning Electron Microscopic Studies\u003c/h2\u003e \u003cp\u003eThe mites were fixed in 2.5% glutaraldehyde solution for overnight. Then they were washed in cacodylate buffer and dehydrated in a graded series of alcohol. Then the samples were infiltrated with amyl acetate and dried to critical point and coated with gold and observed for Scanning Electron Microscopy (SEM) using Carl Zeiss Scanning Electron Microscope MODEL: Gemini; Sigma 300 at 500 (Kilo Volt) KV.\u003c/p\u003e \u003cdiv id=\"Sec4\" class=\"Section3\"\u003e \u003ch2\u003eSequencing Analysis\u003c/h2\u003e \u003cp\u003eFor molecular identification, the DNA of the sample was isolated. The quality of the DNA was evaluated with 1.0% agarose gel. The COI gene was then isolated and amplified using HCO and LCO primers. Resolution was done on agarose gel where a single discrete amplicon band of 700 bp was obtained. Any contamination was removed by purification of the band. The PCR amplicon was subjected to forward and reverse sequencing studies on an ABI 3730xl Genetic Analyzer using a BDT v3.1 Cycle sequencing kit. The consensus sequence was generated with the help of aligner software. BLAST was carried out using the COI gene sequence with the \u0026lsquo;nr\u0026rsquo; database of the NCBI gene bank database. First ten sequence based on their maximum identity scores were selected and then aligned by using the multiple alignment software program Clustal W. RPD database was used to generate the distance matrix. A phylogenetic tree was constructed using MEGA 11.\u003c/p\u003e \u003c/div\u003e \u003c/div\u003e"},{"header":"Results","content":"\u003cp\u003e\u003cstrong\u003eMorphological description\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eDorsoventrally flattened, oval shaped body. Body divided into head, thorax and abdomen. Greenish-black in colour. The carapace is sclerotised and covers the idiostoma. Feeding appendages consist of chelicerae and pedipalp which are attached to the capitulum. Opening of the genital pore is highly sclerotised which is also known as the genital plate. Setae cover the genital plate. Presence of four pairs of jointed appendages. Location of the genital field at the post-ventor part of the body. Morphometric measurements were \u0026ndash; (DP) -\u0026plusmn;443\u0026micro;m, Idiostoma (IS) - \u0026plusmn;467\u0026micro;m, Capitulum (CA)- \u0026plusmn; , Pedipalp (PP) - \u0026plusmn;95\u0026micro;m, 1\u003csup\u003est\u0026nbsp;\u003c/sup\u003epair of leg (I-L) - \u0026plusmn; 380 \u0026micro;m, 2\u003csup\u003end\u003c/sup\u003epair of leg (II-L)- \u0026plusmn; 437 \u0026micro;m, 3\u003csup\u003erd\u0026nbsp;\u003c/sup\u003epair of leg (III-L) - \u0026plusmn; 490 \u0026micro;m, 4\u003csup\u003eth\u0026nbsp;\u003c/sup\u003epair of leg (IV-L) - \u0026plusmn; 631 \u0026micro;m, Carapace (C) - \u0026plusmn; 457 \u0026micro;m\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTaxonomic Summary\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eType Material: \u003cem\u003eUnionicola chelata\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eHost\u003c/strong\u003e: \u003cem\u003eBellamya bengalensis\u003c/em\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eStudy Site:\u003c/strong\u003e\u0026nbsp; Diamond Harbour, South 24 Parganas district, West Bengal\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eLocality:\u003c/strong\u003e\u0026nbsp; Nabasan, West Bengal, India\u003c/p\u003e\n\u003cp\u003e754R+377, Nabasan, Sarisha, West Bengal 743368\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eLatitude:\u003c/strong\u003e 22.255164\u003csup\u003eo\u003c/sup\u003e North\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eLongitude:\u0026nbsp;\u003c/strong\u003e88.19123\u003csup\u003eo\u0026nbsp;\u003c/sup\u003eEast\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSite of Infection:\u003c/strong\u003e Gill\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTotal number of samples observed:\u0026nbsp;\u003c/strong\u003e37\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eNumber of infected samples:\u003c/strong\u003e 23\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePrevalence:\u003c/strong\u003e 23/37 (62.162%)\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSymbiotype:\u003c/strong\u003e BDW/03 is deposited in the museum of Department of Zoology, Diamond Harbour Women\u0026rsquo;s University, Sarisha, Diamond Harbour 743368, West Bengal, India\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eHolotype:\u003c/strong\u003e UC/08 is deposited in the museum of Department of Zoology, Diamond Harbour Women\u0026rsquo;s University, Sarisha, Diamond Harbour 743368, West Bengal, India\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eParatype:\u003c/strong\u003e UC/04, UC/05 and other slides are deposited in the collection of the Parasitology Laboratory, Department of Zoology, Diamond Harbour Women\u0026rsquo;s University, Sarisha, Diamond Harbour 743368, West Bengal, India\u003c/p\u003e\n\u003cp\u003eThe scanning electron microscopic images revealed the dorsal side of the mite, showing the Capitulum (CA), Walking Legs (WL), and the Dorsal Plate (DP), a pair of Pedipalp (PP) and a prominent Genital Ridge (GR). The walking legs showed the presence of bristles. The following figures 2(a-c) are obtained from the Scanning Electron Microscopic study of the mite.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eSequencing results\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eDistance matrix\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe distance matrix was calculated in order to find out the base substitution between the sequences per site. The \u0026ldquo;Maximum Composite Likelihood Model\u0026rdquo; was conducted for analysis (Kimura et al., 1980). This involved about 11 nucleotide sequences. 1\u003csup\u003est\u003c/sup\u003e, 2\u003csup\u003end\u003c/sup\u003e, 3\u003csup\u003erd\u0026nbsp;\u003c/sup\u003eand non-coding positions were included. A total of 775 positions were placed in the final database. Evolutionary analysis was done using MEGA-11(Kumar et al., 2018). The following two tables Table I and Table II were obtained from the sequence studies of the mite. Table I depicts the evolutionary divergence between the sequences and Table II lists the sequences producing similar alignments.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable I: Estimates of the Evolutionary Divergence between sequence\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable II: Sequences producing similar alignments\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003ePhylogenetic Tree\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe evolutionary relationship was derived by using the Tamura-Nei model and Maximum likelihood method (Kimura et al., 1980). Initial phylogenetic trees were obtained for the heuristic search by the application of Bio NJ algorithm Neighbour \u0026ndash; Join to a parirwise distance matrix and then by selecting the topology of the superior log. Total 11 nucleotide sequences were analyzed including the codon positions of 1\u003csup\u003est\u003c/sup\u003e+2\u003csup\u003end\u003c/sup\u003e+3\u003csup\u003erd\u0026nbsp;\u003c/sup\u003enon-coding regions. \u0026nbsp;A total of 775 positions were there in the final dataset. The evolutionary relationship was analysed by the MEGA 11 software (Kumar et al., 2018).\u003c/p\u003e\n\u003cp\u003eThe following table presents a comparison of morphological features between \u003cstrong\u003e\u003cem\u003eUnionicola chelata\u003c/em\u003e\u003c/strong\u003e, described in this paper and the previously discovered similar species of parasitic aquatic mites.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable III: Comparison of the morphological features of \u003cem\u003eU.chelata\u0026nbsp;\u003c/em\u003eand similar species\u003c/strong\u003e\u003c/p\u003e"},{"header":"Discussion","content":"\u003cp\u003eThe present paper deals with three different methods for identification of an aquatic mite collected from freshwater gastropod mollusc \u003cem\u003eBellamya bengalensis.\u003c/em\u003e These identification methods included morphological studies, Scanning Electron Microscopic studies and Sequencing.\u003c/p\u003e \u003cp\u003eThe morphological characters observed using compound microscopy showed differences in some features from the previously identified similar species of mites belonging to the same Genus which is presented in a comparative table.\u003c/p\u003e \u003cp\u003eThe SEM images shows a clear view of the dorsal side of the mite, its walking leg and a prominent genital ridge. These features also indicate that the species is quite different than the other mentioned species of the Genus \u003cem\u003eUnionicola\u003c/em\u003e while its features match to that of \u003cem\u003eUnionicola chelata.\u003c/em\u003e\u003c/p\u003e \u003cp\u003eThe gene sequencing studies are done using the MT COI gene. It has been used to generate a distance matrix and a phylogenetic tree which has revealed its taxonomic position.\u003c/p\u003e \u003cp\u003e \u003cem\u003eUnionicola chelata\u003c/em\u003e has been reported from unionid bivalves so far. In this paper, new geographical area and new host species of the parasitic arthropod has been reported. The morphometric, microscopic and sequencing results have confirmed the species to be \u003cem\u003eUnionicola chelata\u003c/em\u003e. This mite has been reported for the first time from a new host \u003cem\u003eBellamya bengalensis\u003c/em\u003e from South 24 Parganas district, West Bengal. Before, \u003cem\u003eUnionicola savadiensis\u003c/em\u003e was reported from India in freshwater bivalve mussels but \u003cem\u003eUnionicola chelata\u003c/em\u003e have been reported \u003cem\u003eBellamya\u003c/em\u003e sp. for the first time from West Bengal, India.\u003c/p\u003e"},{"header":"Declarations","content":"\u003cp\u003e\u003cstrong\u003eAcknowledgement\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors are grateful to the Department of Zoology, Diamond Harbour Women’s University for granting us the permission to carry out this research work. The authors are grateful to the Department of Zoology, Diamond Harbour Women’s University for granting us the permission to carry out this research work. We extend our thanks to Burdhwan University, for letting us perform the Scanning Electron Microscopic studies in their lab. We are also thankful to Barcode Biosciences, Bangalore for the sequence analysis.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eConflict of Interest\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors of this paper have no conflict of interest regarding the commercial, academic, political, financial and personal aspects.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eAuthors Contributions\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eAll the authors have equally contributed to the successful writing of this paper and the works associated with it.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eFunding\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eNo amount of fund was received during for carrying out any field or lab based work.\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eCompeting Interests\u003c/strong\u003e\u003c/p\u003e\n\u003cp\u003eThe authors have no competing interests \u0026nbsp;\u003c/p\u003e"},{"header":"References","content":"\u003col\u003e\n\u003cli\u003eBaby RL, Hasan I, Kabir KA, Naser MN (2010) Nutrient analysis of some commercially important mollusks of Bangladesh. J. Sci. Res. 2(@): 390-396\u003c/li\u003e\n\u003cli\u003eBaker P, Mann R (1997). The post larval phase of bivalve mollusks: a review of functional ecology and new records of post larval drifting of Chesapeake Bay bivalves. Bull. Mar. Sci\u003cem\u003e.\u003c/em\u003e, \u003cem\u003e61\u003c/em\u003e(2), 409-430.\u003c/li\u003e\n\u003cli\u003eBottger K (1977) The general life cycle of fresh water mites (Hydrachnellae, Acari). Archael. 18: 496\u0026ndash;502. \u003c/li\u003e\n\u003cli\u003eChapurina YE, Bolotov IN, Vidrine MF, Vikhrev IV, Lunn Z, Chan N, Win T, Bespalaya YV, Aksenova OV, Gofarov MY (2021) Taxonomic richness and host range of tropical Asian mussel-associated mite assemblage (Acari: Unionicolidae) with description of a new subgenus and species of parasitic mite from freshwater pearl mussels (Unionida: Margaritiferidae). J. Zool. Syst. Evol. Res\u003cem\u003e.\u003c/em\u003e \u003cem\u003e59\u003c/em\u003e, 613\u0026ndash;634. \u003c/li\u003e\n\u003cli\u003eChapurina Y E, Kondakov A V, Chan N, Vikhrev I V, Bolotov I N, Konopleva E S, Win T, Lunn Z (2022). A new species Unionicola Dimockatax stat. rev.) Haungthayawensis sp. Nov. (Trombidiformes: Unionicolidae) from the freshwater mussel \u003cem\u003eLamellidens generosus\u003c/em\u003e (Gould, 1847) in Myanmar. Ecologica Montenegrina, \u003cem\u003e56\u003c/em\u003e, 28\u0026ndash;39. https://doi.org/10.37828/em.2022.56.4 \u003c/li\u003e\n\u003cli\u003eChapurina YE, Konopleva ES, Vidrine MF, Vikhrev IV, Lunn Z, Chan N, Win T, Kondakov AV, Zubrii NA, Bespalaya YV, (2022). New Molecular-Based Phylogeny of Mussel-Associated Mites Reveals a New Subgenus and Three New Species Representing an Example of a Host-Driven Radiation in Indochina and Confirms the Concept of Division of the Genus \u003cem\u003eUnionicola\u003c/em\u003e Haldeman, 1842 (Acari: Unionicolidae) into Numerous Subgenera. \u003cem\u003eDiversity\u003c/em\u003e. 14(10):848. https://doi.org/10.3390/d14100848 \u003c/li\u003e\n\u003cli\u003eChapurina YE, Kondakov AV, Chan N, Vikhrev IV, Bolotov IN, Konopleva ES, Win T, Lunn Z (2022). A new species \u003cem\u003eUnionicola\u003c/em\u003e (\u003cem\u003eDimockatax\u003c/em\u003e stat. rev.) \u003cem\u003ehaungthayawensis\u003c/em\u003e sp. nov. (Trombidiformes: Unionicolidae) from the freshwater mussel \u003cem\u003eLamellidens generosus\u003c/em\u003e (Gould, 1847) in Myanmar. Ecol. Monten\u003cem\u003e.\u003c/em\u003e \u003cem\u003e56\u003c/em\u003e, 28\u0026ndash;39.\u003c/li\u003e\n\u003cli\u003eDavids C (1973) Hydrobiologia.\u003c/li\u003e\n\u003cli\u003eDing Z, Jin D, Guo J, Yi T (2019). Three new species of the subgenus \u003cem\u003eUnionicola\u003c/em\u003e Haldeman, 1842 (Acari, Hydrachnida, Unionicolidae) from Guizhou, China. Zootaxa \u003cem\u003e4658\u003c/em\u003e, 141\u0026ndash;154.\u003c/li\u003e\n\u003cli\u003eErnsting BR, Edwards DD, Vidrine MF, Cun H (2008) Int J Acarol. \u003cstrong\u003e4\u003c/strong\u003e, 403-407\u003c/li\u003e\n\u003cli\u003eEdwards DD, Vidrine MF (2006) Host specificity among \u003cstrong\u003e\u003cem\u003eUtiionicoln \u003c/em\u003e\u003c/strong\u003espp. (Acari: Unionicolidae) parasitizing freshwater mussels. J. Parasitol. 92(5): Imamura, T. 1953. Water-mites from Gifb Prefecture. J.Fac. Sci., Hokkaido Univ., Ser. VI, Zool. \u003cstrong\u003e11: \u003c/strong\u003e411-471.\u003c/li\u003e\n\u003cli\u003eEdwards DD and Vidrine MF (2013) Mites of freshwater mollusks. Malcolm F. Vidrine, Eunice, Louisiana, 336 p.\u003c/li\u003e\n\u003cli\u003eFaust E C (1924) Schistosomiasis in China: biological and practical aspects.\u003c/li\u003e\n\u003cli\u003eGracia- Cubas A (1963) Sistemica y distribution de los micromole scosrecientes de la; aguna de Terminos, Campeche, Maxico. Boletin mde la Instituto de Geologia, Universidad National Autonomic de Maxcico. 67:1-55\u003c/li\u003e\n\u003cli\u003eGuo X, Ford S E (2016) Infectious diseases of marine molluscs and host responses as revealed by genomic tools. \u003cem\u003ePhilosophical Transactions of the Royal Society B: Biological Sciences\u003c/em\u003e, \u003cem\u003e371\u003c/em\u003e(1689), 20150206.\u003c/li\u003e\n\u003cli\u003eLewisch E, Arnold F, Fuehrer HP, Harl J, Reichart U, Handschuh S, Thielen F, Matbouli EI (2021). 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Org. 70: 123-127.\u003c/li\u003e\n\u003c/ol\u003e"},{"header":"Tables","content":"\u003cp\u003e\u003cstrong\u003eTable I: Estimates of the Evolutionary Divergence between sequence\u003c/strong\u003e\u003c/p\u003e\n\u003ctable\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 68.65pt;border: 1pt solid black;padding: 0in 5.4pt;height: 15.15pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;color:#C10000;'\u003eSAMPLE_B\u0026nbsp;\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.15pt;border-top: 1pt solid black;border-right: 1pt solid black;border-bottom: 1pt solid black;border-image: initial;border-left: none;padding: 0in 5.4pt;height: 15.15pt;vertical-align: top;\"\u003e\n \u003cp 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none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.265\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.960\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.973\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.966\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.265\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.227\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.227\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.227\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.227\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:13px;font-family:\"Arial\",sans-serif;'\u003e0.226\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 37.25pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;height: 15.9pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style='font-size:16px;font-family:\"Times New Roman\",serif;'\u003e\u0026nbsp;\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c/p\u003e\n\u003cp\u003e\u003cstrong\u003eTable II: Sequences producing similar alignments\u003c/strong\u003e\u003c/p\u003e\n\u003ctable\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 79.9pt;border: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eDescription\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.9pt;border-top: 1pt solid black;border-right: 1pt solid black;border-bottom: 1pt solid black;border-image: initial;border-left: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eMax\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eScore\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.95pt;border-top: 1pt solid black;border-right: 1pt solid black;border-bottom: 1pt solid black;border-image: initial;border-left: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eTotal\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eScore\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.05pt;border-top: 1pt solid black;border-right: 1pt solid black;border-bottom: 1pt solid black;border-image: initial;border-left: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eQuery\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eCover\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.7pt;border-top: 1pt solid black;border-right: 1pt solid black;border-bottom: 1pt solid black;border-image: initial;border-left: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eE\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003evalue\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.15pt;border-top: 1pt solid black;border-right: 1pt solid black;border-bottom: 1pt solid black;border-image: initial;border-left: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003ePer.\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eIdent\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 69.15pt;border-top: 1pt solid black;border-right: 1pt solid black;border-bottom: 1pt solid black;border-image: initial;border-left: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cstrong\u003e\u003cspan style='font-family:\"Calibri,Bold\",sans-serif;color:#C10000;'\u003eAccession\u003c/span\u003e\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 79.9pt;border-right: 1pt solid black;border-bottom: 1pt solid black;border-left: 1pt solid black;border-image: initial;border-top: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cem\u003e\u003cspan style=\"color:#0070C1;\"\u003eUnionicola chelata\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"color:#0070C1;\"\u003e\u0026nbsp;isolate 17\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.9pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e627\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.95pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e627\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.05pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e100%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.7pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e1e-174\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e83.87%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 69.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:blue;\"\u003eFJ218009.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 79.9pt;border-right: 1pt solid black;border-bottom: 1pt solid black;border-left: 1pt solid black;border-image: initial;border-top: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cem\u003e\u003cspan style=\"color:#0070C1;\"\u003eUnionicola chelata\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"color:#0070C1;\"\u003e\u0026nbsp;isolate 33\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.9pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e614\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.95pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e614\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.05pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e99%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.7pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e1e-170\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp 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BOLD:ACG4692 BIOUG33431-A05\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.9pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e505\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.95pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e505\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.05pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e99%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.7pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e7e-138\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e80.67%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 69.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:blue;\"\u003eMN362553.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd style=\"width: 79.9pt;border-right: 1pt solid black;border-bottom: 1pt solid black;border-left: 1pt solid black;border-image: initial;border-top: none;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cem\u003e\u003cspan style=\"color:#0070C1;\"\u003eUnionicola\u003c/span\u003e\u003c/em\u003e\u003cspan style=\"color:#0070C1;\"\u003e\u0026nbsp;sp. 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BIOUG27038-D03\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.9pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e486\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.95pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e486\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.05pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e98%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 65.7pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e2e-132\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 66.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:#222222;\"\u003e80.37%\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd style=\"width: 69.15pt;border-top: none;border-left: none;border-bottom: 1pt solid black;border-right: 1pt solid black;padding: 0in 5.4pt;vertical-align: top;\"\u003e\n \u003cp style='margin-top:0in;margin-right:0in;margin-bottom:0in;margin-left:0in;line-height:normal;font-size:15px;font-family:\"Calibri\",sans-serif;text-align:justify;'\u003e\u003cspan style=\"color:blue;\"\u003eMG317695.1\u003c/span\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e\n\u003cp\u003e\u003cstrong\u003eTable III: Comparison of the morphological features of \u003cem\u003eU.chelata\u0026nbsp;\u003c/em\u003eand similar species\u003c/strong\u003e\u003c/p\u003e\n\u003ctable border=\"1\" cellspacing=\"0\" cellpadding=\"0\" width=\"646\"\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.2015503875969%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eS.\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003eNo.\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.65891472868217%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eInfecting Species\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.7984496124031%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eHost\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.333333333333334%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eSite of Infection\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"51.007751937984494%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003eMorphological features\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.2015503875969%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e1.\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.65891472868217%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eU. haungthaya\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.7984496124031%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eLamellidens generous\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.333333333333334%\" valign=\"top\"\u003e\n \u003cp\u003eGill, Mantle\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"51.007751937984494%\" valign=\"top\"\u003e\n \u003cp\u003eDorsum lacking \u0026nbsp; \u0026nbsp; dorsal patelets\u0026nbsp;\u003c/p\u003e\n \u003cp\u003ePresence of distinct margins in the coxal plate\u003c/p\u003e\n \u003cp\u003eFemale genital field (31-50) acetabula\u003c/p\u003e\n \u003cp\u003eMale genital plate(22-33)acetabula\u003c/p\u003e\n \u003cp\u003eStocky and well sclerotised pedipalp\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.2015503875969%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e2.\u003c/strong\u003e\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.65891472868217%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eU. savadiensis\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.7984496124031%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eL.savadienss\u0026nbsp;\u003c/em\u003eand \u003cem\u003eL.generous\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.333333333333334%\" valign=\"top\"\u003e\n \u003cp\u003eGill, Mantle\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"51.007751937984494%\" valign=\"top\"\u003e\n \u003cp\u003eWithout dorsal platelets\u003c/p\u003e\n \u003cp\u003eWithout apperent posterior margin\u003c/p\u003e\n \u003cp\u003e(13-20) both male and female\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.2015503875969%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e3.\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.65891472868217%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eU. generosa\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.7984496124031%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eLamellidens generous\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.333333333333334%\" valign=\"top\"\u003e\n \u003cp\u003eGill, Mantle\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"51.007751937984494%\" valign=\"top\"\u003e\n \u003cp\u003eLacks dorsal platelets\u003c/p\u003e\n \u003cp\u003e15-18 acetabula in males\u003c/p\u003e\n \u003cp\u003e\u003cstrong\u003e\u0026nbsp;\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003ctr\u003e\n \u003ctd width=\"6.2015503875969%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cstrong\u003e4.\u003c/strong\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"15.65891472868217%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eU.chelata\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.7984496124031%\" valign=\"top\"\u003e\n \u003cp\u003e\u003cem\u003eB. bengalensis\u003c/em\u003e\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"13.333333333333334%\" valign=\"top\"\u003e\n \u003cp\u003eGill, Mantle\u003c/p\u003e\n \u003c/td\u003e\n \u003ctd width=\"51.007751937984494%\" valign=\"top\"\u003e\n \u003cp\u003ecarapace is sclerotised\u003c/p\u003e\n \u003cp\u003epedipalp attached to the capitulum\u003c/p\u003e\n \u003cp\u003eOpening of genital pore highly sclerotised\u003c/p\u003e\n \u003c/td\u003e\n \u003c/tr\u003e\n \u003c/tbody\u003e\n\u003c/table\u003e"}],"fulltextSource":"","fullText":"","funders":[],"hasAdminPriorityOnWorkflow":false,"hasManuscriptDocX":true,"hasOptedInToPreprint":true,"hasPassedJournalQc":"","hasAnyPriority":false,"hideJournal":false,"highlight":"","institution":"","isAcceptedByJournal":true,"isAuthorSuppliedPdf":false,"isDeskRejected":"","isHiddenFromSearch":false,"isInQc":false,"isInWorkflow":true,"isPdf":false,"isPdfUpToDate":true,"isWithdrawnOrRetracted":false,"journal":{"display":true,"email":"[email protected]","identity":"journal-of-parasitic-diseases","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"jopd","sideBox":"Learn more about [Journal of Parasitic Diseases](https://www.springer.com/journal/12639)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/jopd/default.aspx","title":"Journal of Parasitic Diseases","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false},"keywords":"Bellamya bengalensis, Unionicola chelata, Morphological characters, SEM, Gene Sequence","lastPublishedDoi":"10.21203/rs.3.rs-3945341/v1","lastPublishedDoiUrl":"https://doi.org/10.21203/rs.3.rs-3945341/v1","license":{"name":"CC BY 4.0","url":"https://creativecommons.org/licenses/by/4.0/"},"manuscriptAbstract":"\u003cp\u003e\u003cem\u003eUnionicola\u003c/em\u003e sp. is a parasitic aquatic mite known to infect freshwater aquatic organisms, especially the malacofauna species and few species of sponges. \u003cem\u003eUnionicola chelata \u003c/em\u003e(Acari: Hydrachnida: Unionicolidae) generally infect the freshwater bivalves of the Genus \u003cem\u003eUnio\u003c/em\u003e sp. \u0026nbsp;They are usually facultative in nature and can be parasitic at any stage of their life cycle. They cause damage to the gills of the host which harms their normal respiration process. The present work portraits the morphological characters, morphometric details, Scanning Electron Microscopy (SEM) images and sequence data of its mitochondrial gene “Cytochrome Oxidase I (COI)” and its taxonomic position was justified by obtaining a phylogenetic tree. The description regarding its morphological, morphometric characters and sequence studies has been presented here. This paper holds the report of a parasitic aquatic mite \u003cem\u003eUnionicola chelata\u003c/em\u003e for the first time from a Gastropod molluscan host \u003cem\u003eBellamya bengalensis \u003c/em\u003e(Lamarck, 1882), from Diamond Harbour, South 24 Parganas, West Bengal, India.\u003c/p\u003e","manuscriptTitle":"Morphometric and molecular identification of Unionicola chelata (Acari: Hydrachnida: Unionicolidae) from Bellamya bengalensis, a gastropod mollusc in South 24 Parganas district, West Bengal, India","msid":"","msnumber":"","nonDraftVersions":[{"code":1,"date":"2024-04-26 20:33:31","doi":"10.21203/rs.3.rs-3945341/v1","editorialEvents":[{"type":"communityComments","content":0},{"type":"decision","content":"Major revisions needed","date":"2024-06-03T00:25:25+00:00","index":"","fulltext":""},{"type":"reviewerAgreed","content":"","date":"2024-04-25T11:45:28+00:00","index":0,"fulltext":""},{"type":"reviewersInvited","content":"","date":"2024-04-22T04:08:03+00:00","index":"","fulltext":""},{"type":"editorAssigned","content":"","date":"2024-02-09T07:10:29+00:00","index":"","fulltext":""},{"type":"submitted","content":"Journal of Parasitic Diseases","date":"2024-02-08T08:10:18+00:00","index":"","fulltext":""}],"status":"published","journal":{"display":true,"email":"[email protected]","identity":"journal-of-parasitic-diseases","isNatureJournal":false,"hasQc":true,"allowDirectSubmit":false,"externalIdentity":"jopd","sideBox":"Learn more about [Journal of Parasitic Diseases](https://www.springer.com/journal/12639)","snPcode":"","submissionUrl":"https://www.editorialmanager.com/jopd/default.aspx","title":"Journal of Parasitic Diseases","twitterHandle":"","acdcEnabled":true,"dfaEnabled":true,"editorialSystem":"em","reportingPortfolio":"Springer Hybrid","inReviewEnabled":true,"inReviewRevisionsEnabled":false}}],"origin":"","ownerIdentity":"6047aa40-05a8-4bb9-b1bc-213664814203","owner":[],"postedDate":"April 26th, 2024","published":true,"recentEditorialEvents":[],"rejectedJournal":[],"revision":"","amendment":"","status":"published-in-journal","subjectAreas":[],"tags":[],"updatedAt":"2024-08-01T17:08:07+00:00","versionOfRecord":{"articleIdentity":"rs-3945341","link":"https://doi.org/10.1007/s12639-024-01704-z","journal":{"identity":"journal-of-parasitic-diseases","isVorOnly":false,"title":"Journal of Parasitic Diseases"},"publishedOn":"2024-07-26 16:15:53","publishedOnDateReadable":"July 26th, 2024"},"versionCreatedAt":"2024-04-26 20:33:31","video":"","vorDoi":"10.1007/s12639-024-01704-z","vorDoiUrl":"https://doi.org/10.1007/s12639-024-01704-z","workflowStages":[]},"version":"v1","identity":"rs-3945341","journalConfig":"researchsquare"},"__N_SSP":true},"page":"/article/[identity]/[[...version]]","query":{"redirect":"/article/rs-3945341","identity":"rs-3945341","version":["v1"]},"buildId":"qtupq5eGEP_6zYnWcrvyt","isFallback":false,"isExperimentalCompile":false,"dynamicIds":[84888],"gssp":true,"scriptLoader":[]}