Disinfectant/Antiseptic Resistance Genes of Staphylococci and Serotyping of Clinical Staphylococcus aureus Isolates in Hatay Region.

Aim: This study was performed for the aim of determination of the resistance against antiseptics and disinfectants of staphylococcii and detection of dominant Staphylococcus aureus serotype in Hatay Region. Method: The total of 429 staphylococcal isolates which were isolated from the clinical specimens in the Central Laboratory of the Hatay Mustafa Kemal University Health Application and Research Hospital between 2017-2018, included in this study. The antiseptic and disinfectant susceptibility of the identified strains was determined genotypically. Staphylococcus aureus strains were isolated and subjected to Pulse Field Gel Electrophoresis and spa sequence typing regarding to clonal and phylogenetical relationships. Findings: While the 43.1% of the isolates were S. aureus , the others identified as coagulase negative staphylococcii. The number of metisilline resistant S. aureus isolates was 104. It was determined that 81 (77.88%) of these isolates were carried qacA/B and/or smr genes. t223 were found to be the dominant spa serotype. Conclusion: It was observed that a decreased susceptibility in clinical staphylococcal isolates against the most commonly used antiseptic and disinfectant agents, quaterner ammonium compounds and chlorhexidine, in healthcare facilities. While the spa serotype t030 was dominant in our country general, in Hatay region the spa serotype t223 was came forward the dominant clon. Since there are many Syrian immigrants live in the Hatay region due to its geographical location, being the dominant clone of a different serotype has an epidemiologically significant importance. It is thought that this finding may affect the antibiotic, antiseptic/disinfectant resistance profiles of staphylococci in this region.